Effect of salicylic acid, methyl jasmonate, ethephon and cantharidin on anthraquinone production by Rubia cordifolia callus cultures transformed with the rolB and rolC genes
It has been suggested that the rol genes of Agrobacterium rhizogenes could play an essential role in the activation of secondary metabolite production in plant transformed cultures. This study investigated whether the content of anthraquinone phytoalexins was changed in callus cultures of Rubia cord...
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| Veröffentlicht in: | Journal of biotechnology 2002-08, Vol.97 (3), p.213-221 |
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| creator | Bulgakov, V.P Tchernoded, G.K Mischenko, N.P Khodakovskaya, M.V Glazunov, V.P Radchenko, S.V Zvereva, E.V Fedoreyev, S.A Zhuravlev, Yu.N |
| description | It has been suggested that the
rol genes of
Agrobacterium rhizogenes could play an essential role in the activation of secondary metabolite production in plant transformed cultures. This study investigated whether the content of anthraquinone phytoalexins was changed in callus cultures of
Rubia cordifolia transgenic for the 35S-
rolB and 35S-
rolC genes in comparison with a non-transformed callus culture. The anthraquinone content was shown to be significantly increased in transgenic cultures, thus providing further evidence that the
rol-gene transformation can be used for the activation of secondary metabolism in plant cells. Methyl jasmonate and salicylic acid strongly increased anthraquinone accumulation in both transgenic and non-transgenic
R. cordifolia calluses, whereas ethephon did not. A treatment of the cultures by cantharidin, the protein phosphatase 2A inhibitor, resulted in massive induction of anthraquinone accumulation in the transgenic cultures only. We suggest the involvement of a cantharidin-sensitive protein phosphorylation mechanism in anthraquinone biosynthesis in transgenic cultures. |
| doi_str_mv | 10.1016/S0168-1656(02)00067-6 |
| format | Article |
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rol genes of
Agrobacterium rhizogenes could play an essential role in the activation of secondary metabolite production in plant transformed cultures. This study investigated whether the content of anthraquinone phytoalexins was changed in callus cultures of
Rubia cordifolia transgenic for the 35S-
rolB and 35S-
rolC genes in comparison with a non-transformed callus culture. The anthraquinone content was shown to be significantly increased in transgenic cultures, thus providing further evidence that the
rol-gene transformation can be used for the activation of secondary metabolism in plant cells. Methyl jasmonate and salicylic acid strongly increased anthraquinone accumulation in both transgenic and non-transgenic
R. cordifolia calluses, whereas ethephon did not. A treatment of the cultures by cantharidin, the protein phosphatase 2A inhibitor, resulted in massive induction of anthraquinone accumulation in the transgenic cultures only. We suggest the involvement of a cantharidin-sensitive protein phosphorylation mechanism in anthraquinone biosynthesis in transgenic cultures.</description><identifier>ISSN: 0168-1656</identifier><identifier>EISSN: 1873-4863</identifier><identifier>DOI: 10.1016/S0168-1656(02)00067-6</identifier><identifier>PMID: 12084477</identifier><identifier>CODEN: JBITD4</identifier><language>eng</language><publisher>Lausanne: Elsevier B.V</publisher><subject>Acetates - metabolism ; Acetates - pharmacology ; Anthraquinones ; Anthraquinones - isolation & purification ; Anthraquinones - metabolism ; beta-Glucosidase - genetics ; Callus culture ; Cantharidin - metabolism ; Cantharidin - pharmacology ; Cyclopentanes - metabolism ; Cyclopentanes - pharmacology ; Enzyme Inhibitors - metabolism ; Enzyme Inhibitors - pharmacology ; Gene Expression ; Gene Expression Regulation ; Genes, Bacterial ; Oncogene Proteins - genetics ; Oncogenes ; Organophosphorus Compounds - metabolism ; Organophosphorus Compounds - pharmacology ; Oxylipins ; Plant Growth Regulators - metabolism ; Plant Growth Regulators - pharmacology ; Plants, Genetically Modified - drug effects ; Plants, Genetically Modified - metabolism ; Rhizobium - genetics ; rolB and rolC genes ; Rubia cordifolia ; Rubiaceae - drug effects ; Rubiaceae - genetics ; Rubiaceae - metabolism ; Salicylic Acid - metabolism ; Salicylic Acid - pharmacology ; Sensitivity and Specificity</subject><ispartof>Journal of biotechnology, 2002-08, Vol.97 (3), p.213-221</ispartof><rights>2002 Elsevier Science B.V.</rights><rights>2003 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c422t-17a2e490dc0507fd766214c7addc9cdd5d4755bcdb29ca0a7cb7048f1cfca0d23</citedby><cites>FETCH-LOGICAL-c422t-17a2e490dc0507fd766214c7addc9cdd5d4755bcdb29ca0a7cb7048f1cfca0d23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0168-1656(02)00067-6$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13781393$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12084477$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bulgakov, V.P</creatorcontrib><creatorcontrib>Tchernoded, G.K</creatorcontrib><creatorcontrib>Mischenko, N.P</creatorcontrib><creatorcontrib>Khodakovskaya, M.V</creatorcontrib><creatorcontrib>Glazunov, V.P</creatorcontrib><creatorcontrib>Radchenko, S.V</creatorcontrib><creatorcontrib>Zvereva, E.V</creatorcontrib><creatorcontrib>Fedoreyev, S.A</creatorcontrib><creatorcontrib>Zhuravlev, Yu.N</creatorcontrib><title>Effect of salicylic acid, methyl jasmonate, ethephon and cantharidin on anthraquinone production by Rubia cordifolia callus cultures transformed with the rolB and rolC genes</title><title>Journal of biotechnology</title><addtitle>J Biotechnol</addtitle><description>It has been suggested that the
rol genes of
Agrobacterium rhizogenes could play an essential role in the activation of secondary metabolite production in plant transformed cultures. This study investigated whether the content of anthraquinone phytoalexins was changed in callus cultures of
Rubia cordifolia transgenic for the 35S-
rolB and 35S-
rolC genes in comparison with a non-transformed callus culture. The anthraquinone content was shown to be significantly increased in transgenic cultures, thus providing further evidence that the
rol-gene transformation can be used for the activation of secondary metabolism in plant cells. Methyl jasmonate and salicylic acid strongly increased anthraquinone accumulation in both transgenic and non-transgenic
R. cordifolia calluses, whereas ethephon did not. A treatment of the cultures by cantharidin, the protein phosphatase 2A inhibitor, resulted in massive induction of anthraquinone accumulation in the transgenic cultures only. We suggest the involvement of a cantharidin-sensitive protein phosphorylation mechanism in anthraquinone biosynthesis in transgenic cultures.</description><subject>Acetates - metabolism</subject><subject>Acetates - pharmacology</subject><subject>Anthraquinones</subject><subject>Anthraquinones - isolation & purification</subject><subject>Anthraquinones - metabolism</subject><subject>beta-Glucosidase - genetics</subject><subject>Callus culture</subject><subject>Cantharidin - metabolism</subject><subject>Cantharidin - pharmacology</subject><subject>Cyclopentanes - metabolism</subject><subject>Cyclopentanes - pharmacology</subject><subject>Enzyme Inhibitors - metabolism</subject><subject>Enzyme Inhibitors - pharmacology</subject><subject>Gene Expression</subject><subject>Gene Expression Regulation</subject><subject>Genes, Bacterial</subject><subject>Oncogene Proteins - genetics</subject><subject>Oncogenes</subject><subject>Organophosphorus Compounds - metabolism</subject><subject>Organophosphorus Compounds - pharmacology</subject><subject>Oxylipins</subject><subject>Plant Growth Regulators - metabolism</subject><subject>Plant Growth Regulators - pharmacology</subject><subject>Plants, Genetically Modified - drug effects</subject><subject>Plants, Genetically Modified - metabolism</subject><subject>Rhizobium - genetics</subject><subject>rolB and rolC genes</subject><subject>Rubia cordifolia</subject><subject>Rubiaceae - drug effects</subject><subject>Rubiaceae - genetics</subject><subject>Rubiaceae - metabolism</subject><subject>Salicylic Acid - metabolism</subject><subject>Salicylic Acid - pharmacology</subject><subject>Sensitivity and Specificity</subject><issn>0168-1656</issn><issn>1873-4863</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFktuOFCEQhjtG446rj6DhRqPJtgJNQ_fVRifrIdnExMM1oQuw2TAwC7RmHsp3lDnEvdwLoCi-qoL6aZrnBL8lmPB33-s0tIT3_DWmbzDGXLT8QbMig-haNvDuYbP6j5w1T3K-qRAbe_K4OSMUD4wJsWr-XllroKBoUVbewa4OpMDpC7QxZd55dKPyJgZVzAWqDrOdY0AqaAQqlFklp11AB1eZk7pdXIjBoG2KeoHi6sG0Q9-WySkEMWlno9-byvslI1h8WZLJqCQVso1pYzT648qMaiGUov9wqFSNNfplgslPm0dW-Wyendbz5ufHqx_rz-31109f1u-vW2CUlpYIRQ0bsQbcY2G14JwSBkJpDSNo3Wsm-n4CPdERFFYCJoHZYAnYutW0O29eHfPWd9wuJhe5cRmM9yqYuGQpyNB3dBjuBcnABOV4n7E_gpBizslYuU1uo9JOEiz3gsqDoHKvlsRUHgSVvMa9OBVYptqdu6iTghV4eQJUrm21tZPg8h3XiYF0Y1e5yyNnat9-O5NkBmcCGO1S_QBSR3fPVf4BIdnBcQ</recordid><startdate>20020828</startdate><enddate>20020828</enddate><creator>Bulgakov, V.P</creator><creator>Tchernoded, G.K</creator><creator>Mischenko, N.P</creator><creator>Khodakovskaya, M.V</creator><creator>Glazunov, V.P</creator><creator>Radchenko, S.V</creator><creator>Zvereva, E.V</creator><creator>Fedoreyev, S.A</creator><creator>Zhuravlev, Yu.N</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20020828</creationdate><title>Effect of salicylic acid, methyl jasmonate, ethephon and cantharidin on anthraquinone production by Rubia cordifolia callus cultures transformed with the rolB and rolC genes</title><author>Bulgakov, V.P ; Tchernoded, G.K ; Mischenko, N.P ; Khodakovskaya, M.V ; Glazunov, V.P ; Radchenko, S.V ; Zvereva, E.V ; Fedoreyev, S.A ; Zhuravlev, Yu.N</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c422t-17a2e490dc0507fd766214c7addc9cdd5d4755bcdb29ca0a7cb7048f1cfca0d23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Acetates - metabolism</topic><topic>Acetates - pharmacology</topic><topic>Anthraquinones</topic><topic>Anthraquinones - isolation & purification</topic><topic>Anthraquinones - metabolism</topic><topic>beta-Glucosidase - genetics</topic><topic>Callus culture</topic><topic>Cantharidin - metabolism</topic><topic>Cantharidin - pharmacology</topic><topic>Cyclopentanes - metabolism</topic><topic>Cyclopentanes - pharmacology</topic><topic>Enzyme Inhibitors - metabolism</topic><topic>Enzyme Inhibitors - pharmacology</topic><topic>Gene Expression</topic><topic>Gene Expression Regulation</topic><topic>Genes, Bacterial</topic><topic>Oncogene Proteins - genetics</topic><topic>Oncogenes</topic><topic>Organophosphorus Compounds - metabolism</topic><topic>Organophosphorus Compounds - pharmacology</topic><topic>Oxylipins</topic><topic>Plant Growth Regulators - metabolism</topic><topic>Plant Growth Regulators - pharmacology</topic><topic>Plants, Genetically Modified - drug effects</topic><topic>Plants, Genetically Modified - metabolism</topic><topic>Rhizobium - genetics</topic><topic>rolB and rolC genes</topic><topic>Rubia cordifolia</topic><topic>Rubiaceae - drug effects</topic><topic>Rubiaceae - genetics</topic><topic>Rubiaceae - metabolism</topic><topic>Salicylic Acid - metabolism</topic><topic>Salicylic Acid - pharmacology</topic><topic>Sensitivity and Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bulgakov, V.P</creatorcontrib><creatorcontrib>Tchernoded, G.K</creatorcontrib><creatorcontrib>Mischenko, N.P</creatorcontrib><creatorcontrib>Khodakovskaya, M.V</creatorcontrib><creatorcontrib>Glazunov, V.P</creatorcontrib><creatorcontrib>Radchenko, S.V</creatorcontrib><creatorcontrib>Zvereva, E.V</creatorcontrib><creatorcontrib>Fedoreyev, S.A</creatorcontrib><creatorcontrib>Zhuravlev, Yu.N</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bulgakov, V.P</au><au>Tchernoded, G.K</au><au>Mischenko, N.P</au><au>Khodakovskaya, M.V</au><au>Glazunov, V.P</au><au>Radchenko, S.V</au><au>Zvereva, E.V</au><au>Fedoreyev, S.A</au><au>Zhuravlev, Yu.N</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of salicylic acid, methyl jasmonate, ethephon and cantharidin on anthraquinone production by Rubia cordifolia callus cultures transformed with the rolB and rolC genes</atitle><jtitle>Journal of biotechnology</jtitle><addtitle>J Biotechnol</addtitle><date>2002-08-28</date><risdate>2002</risdate><volume>97</volume><issue>3</issue><spage>213</spage><epage>221</epage><pages>213-221</pages><issn>0168-1656</issn><eissn>1873-4863</eissn><coden>JBITD4</coden><abstract>It has been suggested that the
rol genes of
Agrobacterium rhizogenes could play an essential role in the activation of secondary metabolite production in plant transformed cultures. This study investigated whether the content of anthraquinone phytoalexins was changed in callus cultures of
Rubia cordifolia transgenic for the 35S-
rolB and 35S-
rolC genes in comparison with a non-transformed callus culture. The anthraquinone content was shown to be significantly increased in transgenic cultures, thus providing further evidence that the
rol-gene transformation can be used for the activation of secondary metabolism in plant cells. Methyl jasmonate and salicylic acid strongly increased anthraquinone accumulation in both transgenic and non-transgenic
R. cordifolia calluses, whereas ethephon did not. A treatment of the cultures by cantharidin, the protein phosphatase 2A inhibitor, resulted in massive induction of anthraquinone accumulation in the transgenic cultures only. We suggest the involvement of a cantharidin-sensitive protein phosphorylation mechanism in anthraquinone biosynthesis in transgenic cultures.</abstract><cop>Lausanne</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>12084477</pmid><doi>10.1016/S0168-1656(02)00067-6</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Journal of biotechnology, 2002-08, Vol.97 (3), p.213-221 |
| issn | 0168-1656 1873-4863 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Acetates - metabolism Acetates - pharmacology Anthraquinones Anthraquinones - isolation & purification Anthraquinones - metabolism beta-Glucosidase - genetics Callus culture Cantharidin - metabolism Cantharidin - pharmacology Cyclopentanes - metabolism Cyclopentanes - pharmacology Enzyme Inhibitors - metabolism Enzyme Inhibitors - pharmacology Gene Expression Gene Expression Regulation Genes, Bacterial Oncogene Proteins - genetics Oncogenes Organophosphorus Compounds - metabolism Organophosphorus Compounds - pharmacology Oxylipins Plant Growth Regulators - metabolism Plant Growth Regulators - pharmacology Plants, Genetically Modified - drug effects Plants, Genetically Modified - metabolism Rhizobium - genetics rolB and rolC genes Rubia cordifolia Rubiaceae - drug effects Rubiaceae - genetics Rubiaceae - metabolism Salicylic Acid - metabolism Salicylic Acid - pharmacology Sensitivity and Specificity |
| title | Effect of salicylic acid, methyl jasmonate, ethephon and cantharidin on anthraquinone production by Rubia cordifolia callus cultures transformed with the rolB and rolC genes |
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