Expression of Sialyltransferase Family Members in Cervix Squamous Cell Carcinoma Correlates with Lymph Node Metastasis

Objective. Altered messenger ribonucleic acid (mRNA) expression of the four sialyltransferases (STs including ST3Gal I, ST3Gal III, ST3Gal IV, and ST6Gal I) is important in squamous cell carcinoma of the cervix. This study further investigates their changes in mRNA expression of the four STs in FIGO...

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Veröffentlicht in:Gynecologic oncology 2002-07, Vol.86 (1), p.45-52
Hauptverfasser: Wang, Peng-Hui, Li, Ywan Feng, Juang, Chi-Mou, Lee, Yan-Ru, Chao, Hsiang-Tai, Ng, Heung-Tat, Tsai, Ying-Chieh, Yuan, Chiou-Chung
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container_end_page 52
container_issue 1
container_start_page 45
container_title Gynecologic oncology
container_volume 86
creator Wang, Peng-Hui
Li, Ywan Feng
Juang, Chi-Mou
Lee, Yan-Ru
Chao, Hsiang-Tai
Ng, Heung-Tat
Tsai, Ying-Chieh
Yuan, Chiou-Chung
description Objective. Altered messenger ribonucleic acid (mRNA) expression of the four sialyltransferases (STs including ST3Gal I, ST3Gal III, ST3Gal IV, and ST6Gal I) is important in squamous cell carcinoma of the cervix. This study further investigates their changes in mRNA expression of the four STs in FIGO stage IB1 squamous cell carcinoma to assess the extent of sialylation associated with lymph node metastases. Methods. Alterations in ST mRNA expression in FIGO IB1 cervical squamous cell carcinomas (n = 79) were examined by semiquantitative reverse transcription–polymerase chain reaction. Results. Both ST6Gal I mRNA and ST3Gal III mRNA expressions were significantly increased in patients with lymph node metastases compared to those without lymph node metastases (P = 0.002 and P = 0.001, respectively, Mann–Whitney U test). Using receiver operating characteristic curves of ST ratio index for accuracy comparison of lymph node metastases, ST3Gal III and ST6Gal I were observed to be fairly interchangeable (area under the curve (AUC) of 3Gal I = 0.810; AUC of 6Gal I = 0.786, significance of difference between AUC = 0.810). High ST6Gal I expression was associated with other invasive properties of cervical cancer, such as deep stromal invasion and presence of lymph–vascular space involvement. ST6Gal I expression seemed to be more enhanced in bigger tumors. Conclusions. Our results suggested that ST3Gal III and ST6Gal I were of importance for the lymph node metastases in FIGO IB1 cervical cancer patients; more specifically, overexpression of ST6Gal I was of crucial relevance for the presence of poor prognostic factors, such as deep stromal invasion and lymph–vascular space involvement and lymph node metastases.
doi_str_mv 10.1006/gyno.2002.6714
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Altered messenger ribonucleic acid (mRNA) expression of the four sialyltransferases (STs including ST3Gal I, ST3Gal III, ST3Gal IV, and ST6Gal I) is important in squamous cell carcinoma of the cervix. This study further investigates their changes in mRNA expression of the four STs in FIGO stage IB1 squamous cell carcinoma to assess the extent of sialylation associated with lymph node metastases. Methods. Alterations in ST mRNA expression in FIGO IB1 cervical squamous cell carcinomas (n = 79) were examined by semiquantitative reverse transcription–polymerase chain reaction. Results. Both ST6Gal I mRNA and ST3Gal III mRNA expressions were significantly increased in patients with lymph node metastases compared to those without lymph node metastases (P = 0.002 and P = 0.001, respectively, Mann–Whitney U test). Using receiver operating characteristic curves of ST ratio index for accuracy comparison of lymph node metastases, ST3Gal III and ST6Gal I were observed to be fairly interchangeable (area under the curve (AUC) of 3Gal I = 0.810; AUC of 6Gal I = 0.786, significance of difference between AUC = 0.810). High ST6Gal I expression was associated with other invasive properties of cervical cancer, such as deep stromal invasion and presence of lymph–vascular space involvement. ST6Gal I expression seemed to be more enhanced in bigger tumors. Conclusions. Our results suggested that ST3Gal III and ST6Gal I were of importance for the lymph node metastases in FIGO IB1 cervical cancer patients; more specifically, overexpression of ST6Gal I was of crucial relevance for the presence of poor prognostic factors, such as deep stromal invasion and lymph–vascular space involvement and lymph node metastases.</description><identifier>ISSN: 0090-8258</identifier><identifier>EISSN: 1095-6859</identifier><identifier>DOI: 10.1006/gyno.2002.6714</identifier><identifier>PMID: 12079299</identifier><identifier>CODEN: GYNOA3</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Biological and medical sciences ; Carcinoma, Squamous Cell - enzymology ; Carcinoma, Squamous Cell - pathology ; Female ; Female genital diseases ; Gynecology. Andrology. Obstetrics ; Humans ; Isoenzymes - biosynthesis ; Isoenzymes - genetics ; Lymph Nodes - pathology ; Lymphatic Metastasis ; Medical sciences ; Neoplasm Staging ; Prospective Studies ; Regression Analysis ; Reverse Transcriptase Polymerase Chain Reaction ; reverse transcription–polymerase chain reaction (RT-PCR) ; RNA, Messenger - biosynthesis ; RNA, Messenger - genetics ; ROC Curve ; sialyltransferase ; Sialyltransferases - biosynthesis ; Sialyltransferases - genetics ; squamous cell carcinoma of the cervix ; Tropical medicine ; Tumors ; Uterine Cervical Neoplasms - enzymology ; Uterine Cervical Neoplasms - pathology</subject><ispartof>Gynecologic oncology, 2002-07, Vol.86 (1), p.45-52</ispartof><rights>2002 Elsevier Science (USA)</rights><rights>2002 INIST-CNRS</rights><rights>(c) 2002 Elsevier Science (USA).</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c370t-dec2543c2a2c967443dc7fe09a928e300c47c425d789ad100b9969184fbdc2083</citedby><cites>FETCH-LOGICAL-c370t-dec2543c2a2c967443dc7fe09a928e300c47c425d789ad100b9969184fbdc2083</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/gyno.2002.6714$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=13823810$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12079299$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Peng-Hui</creatorcontrib><creatorcontrib>Li, Ywan Feng</creatorcontrib><creatorcontrib>Juang, Chi-Mou</creatorcontrib><creatorcontrib>Lee, Yan-Ru</creatorcontrib><creatorcontrib>Chao, Hsiang-Tai</creatorcontrib><creatorcontrib>Ng, Heung-Tat</creatorcontrib><creatorcontrib>Tsai, Ying-Chieh</creatorcontrib><creatorcontrib>Yuan, Chiou-Chung</creatorcontrib><title>Expression of Sialyltransferase Family Members in Cervix Squamous Cell Carcinoma Correlates with Lymph Node Metastasis</title><title>Gynecologic oncology</title><addtitle>Gynecol Oncol</addtitle><description>Objective. Altered messenger ribonucleic acid (mRNA) expression of the four sialyltransferases (STs including ST3Gal I, ST3Gal III, ST3Gal IV, and ST6Gal I) is important in squamous cell carcinoma of the cervix. This study further investigates their changes in mRNA expression of the four STs in FIGO stage IB1 squamous cell carcinoma to assess the extent of sialylation associated with lymph node metastases. Methods. Alterations in ST mRNA expression in FIGO IB1 cervical squamous cell carcinomas (n = 79) were examined by semiquantitative reverse transcription–polymerase chain reaction. Results. Both ST6Gal I mRNA and ST3Gal III mRNA expressions were significantly increased in patients with lymph node metastases compared to those without lymph node metastases (P = 0.002 and P = 0.001, respectively, Mann–Whitney U test). Using receiver operating characteristic curves of ST ratio index for accuracy comparison of lymph node metastases, ST3Gal III and ST6Gal I were observed to be fairly interchangeable (area under the curve (AUC) of 3Gal I = 0.810; AUC of 6Gal I = 0.786, significance of difference between AUC = 0.810). High ST6Gal I expression was associated with other invasive properties of cervical cancer, such as deep stromal invasion and presence of lymph–vascular space involvement. ST6Gal I expression seemed to be more enhanced in bigger tumors. Conclusions. Our results suggested that ST3Gal III and ST6Gal I were of importance for the lymph node metastases in FIGO IB1 cervical cancer patients; more specifically, overexpression of ST6Gal I was of crucial relevance for the presence of poor prognostic factors, such as deep stromal invasion and lymph–vascular space involvement and lymph node metastases.</description><subject>Biological and medical sciences</subject><subject>Carcinoma, Squamous Cell - enzymology</subject><subject>Carcinoma, Squamous Cell - pathology</subject><subject>Female</subject><subject>Female genital diseases</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Humans</subject><subject>Isoenzymes - biosynthesis</subject><subject>Isoenzymes - genetics</subject><subject>Lymph Nodes - pathology</subject><subject>Lymphatic Metastasis</subject><subject>Medical sciences</subject><subject>Neoplasm Staging</subject><subject>Prospective Studies</subject><subject>Regression Analysis</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>reverse transcription–polymerase chain reaction (RT-PCR)</subject><subject>RNA, Messenger - biosynthesis</subject><subject>RNA, Messenger - genetics</subject><subject>ROC Curve</subject><subject>sialyltransferase</subject><subject>Sialyltransferases - biosynthesis</subject><subject>Sialyltransferases - genetics</subject><subject>squamous cell carcinoma of the cervix</subject><subject>Tropical medicine</subject><subject>Tumors</subject><subject>Uterine Cervical Neoplasms - enzymology</subject><subject>Uterine Cervical Neoplasms - pathology</subject><issn>0090-8258</issn><issn>1095-6859</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kE2LFDEQhoMo7rh69Si56K3HSvorOUqzq8Koh9VzyCTVbqS7M5vqGbf_vWlmYE9CoAg89dbLw9hbAVsB0Hz8vUxxKwHktmlF9YxtBOi6aFStn7MNgIZCyVpdsVdEfwCgBCFfsishodVS6w073TweEhKFOPHY87tgh2WYk52ox2QJ-a0dw7DwbzjuMREPE-8wncIjv3s42jEeKf-HgXc2uTDF0fIupoSDnZH43zDf890yHu759-gxh8yW8gv0mr3o7UD45jKv2a_bm5_dl2L34_PX7tOucGULc-HRyboqnbTS6aatqtK7tkfQVkuFJYCrWlfJ2rdKW5-F7LVutFBVv_dOgiqv2Ydz7iHFhyPSbMZALhe2E-bups2srmrI4PYMuhSJEvbmkMJo02IEmNW0WU2b1bRZTeeFd5fk435E_4Rf1Gbg_QWw5OzQZ6cu0BNXKlkqsV5WZw6zh1PAZMgFnBz6kNDNxsfwvw7_ALeYm4s</recordid><startdate>20020701</startdate><enddate>20020701</enddate><creator>Wang, Peng-Hui</creator><creator>Li, Ywan Feng</creator><creator>Juang, Chi-Mou</creator><creator>Lee, Yan-Ru</creator><creator>Chao, Hsiang-Tai</creator><creator>Ng, Heung-Tat</creator><creator>Tsai, Ying-Chieh</creator><creator>Yuan, Chiou-Chung</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20020701</creationdate><title>Expression of Sialyltransferase Family Members in Cervix Squamous Cell Carcinoma Correlates with Lymph Node Metastasis</title><author>Wang, Peng-Hui ; Li, Ywan Feng ; Juang, Chi-Mou ; Lee, Yan-Ru ; Chao, Hsiang-Tai ; Ng, Heung-Tat ; Tsai, Ying-Chieh ; Yuan, Chiou-Chung</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c370t-dec2543c2a2c967443dc7fe09a928e300c47c425d789ad100b9969184fbdc2083</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Biological and medical sciences</topic><topic>Carcinoma, Squamous Cell - enzymology</topic><topic>Carcinoma, Squamous Cell - pathology</topic><topic>Female</topic><topic>Female genital diseases</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>Humans</topic><topic>Isoenzymes - biosynthesis</topic><topic>Isoenzymes - genetics</topic><topic>Lymph Nodes - pathology</topic><topic>Lymphatic Metastasis</topic><topic>Medical sciences</topic><topic>Neoplasm Staging</topic><topic>Prospective Studies</topic><topic>Regression Analysis</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>reverse transcription–polymerase chain reaction (RT-PCR)</topic><topic>RNA, Messenger - biosynthesis</topic><topic>RNA, Messenger - genetics</topic><topic>ROC Curve</topic><topic>sialyltransferase</topic><topic>Sialyltransferases - biosynthesis</topic><topic>Sialyltransferases - genetics</topic><topic>squamous cell carcinoma of the cervix</topic><topic>Tropical medicine</topic><topic>Tumors</topic><topic>Uterine Cervical Neoplasms - enzymology</topic><topic>Uterine Cervical Neoplasms - pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Peng-Hui</creatorcontrib><creatorcontrib>Li, Ywan Feng</creatorcontrib><creatorcontrib>Juang, Chi-Mou</creatorcontrib><creatorcontrib>Lee, Yan-Ru</creatorcontrib><creatorcontrib>Chao, Hsiang-Tai</creatorcontrib><creatorcontrib>Ng, Heung-Tat</creatorcontrib><creatorcontrib>Tsai, Ying-Chieh</creatorcontrib><creatorcontrib>Yuan, Chiou-Chung</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Gynecologic oncology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Peng-Hui</au><au>Li, Ywan Feng</au><au>Juang, Chi-Mou</au><au>Lee, Yan-Ru</au><au>Chao, Hsiang-Tai</au><au>Ng, Heung-Tat</au><au>Tsai, Ying-Chieh</au><au>Yuan, Chiou-Chung</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of Sialyltransferase Family Members in Cervix Squamous Cell Carcinoma Correlates with Lymph Node Metastasis</atitle><jtitle>Gynecologic oncology</jtitle><addtitle>Gynecol Oncol</addtitle><date>2002-07-01</date><risdate>2002</risdate><volume>86</volume><issue>1</issue><spage>45</spage><epage>52</epage><pages>45-52</pages><issn>0090-8258</issn><eissn>1095-6859</eissn><coden>GYNOA3</coden><abstract>Objective. Altered messenger ribonucleic acid (mRNA) expression of the four sialyltransferases (STs including ST3Gal I, ST3Gal III, ST3Gal IV, and ST6Gal I) is important in squamous cell carcinoma of the cervix. This study further investigates their changes in mRNA expression of the four STs in FIGO stage IB1 squamous cell carcinoma to assess the extent of sialylation associated with lymph node metastases. Methods. Alterations in ST mRNA expression in FIGO IB1 cervical squamous cell carcinomas (n = 79) were examined by semiquantitative reverse transcription–polymerase chain reaction. Results. Both ST6Gal I mRNA and ST3Gal III mRNA expressions were significantly increased in patients with lymph node metastases compared to those without lymph node metastases (P = 0.002 and P = 0.001, respectively, Mann–Whitney U test). Using receiver operating characteristic curves of ST ratio index for accuracy comparison of lymph node metastases, ST3Gal III and ST6Gal I were observed to be fairly interchangeable (area under the curve (AUC) of 3Gal I = 0.810; AUC of 6Gal I = 0.786, significance of difference between AUC = 0.810). High ST6Gal I expression was associated with other invasive properties of cervical cancer, such as deep stromal invasion and presence of lymph–vascular space involvement. ST6Gal I expression seemed to be more enhanced in bigger tumors. Conclusions. Our results suggested that ST3Gal III and ST6Gal I were of importance for the lymph node metastases in FIGO IB1 cervical cancer patients; more specifically, overexpression of ST6Gal I was of crucial relevance for the presence of poor prognostic factors, such as deep stromal invasion and lymph–vascular space involvement and lymph node metastases.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>12079299</pmid><doi>10.1006/gyno.2002.6714</doi><tpages>8</tpages></addata></record>
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subjects Biological and medical sciences
Carcinoma, Squamous Cell - enzymology
Carcinoma, Squamous Cell - pathology
Female
Female genital diseases
Gynecology. Andrology. Obstetrics
Humans
Isoenzymes - biosynthesis
Isoenzymes - genetics
Lymph Nodes - pathology
Lymphatic Metastasis
Medical sciences
Neoplasm Staging
Prospective Studies
Regression Analysis
Reverse Transcriptase Polymerase Chain Reaction
reverse transcription–polymerase chain reaction (RT-PCR)
RNA, Messenger - biosynthesis
RNA, Messenger - genetics
ROC Curve
sialyltransferase
Sialyltransferases - biosynthesis
Sialyltransferases - genetics
squamous cell carcinoma of the cervix
Tropical medicine
Tumors
Uterine Cervical Neoplasms - enzymology
Uterine Cervical Neoplasms - pathology
title Expression of Sialyltransferase Family Members in Cervix Squamous Cell Carcinoma Correlates with Lymph Node Metastasis
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