Comparative Testis Morphometry and Seminiferous Epithelium Cycle Length in Donkeys and Mules
The mule ( Equus mulus mulus ) is a sterile hybrid domestic animal that results from the breeding of a male donkey ( Equus asinus ) to a female horse ( Equus caballus ). Usually, spermatogenesis in mules does not advance beyond spermatocytes. In the present study, we performed a comparative and more...
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| Veröffentlicht in: | Biology of reproduction 2002-07, Vol.67 (1), p.247-255 |
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| description | The mule ( Equus mulus mulus ) is a sterile hybrid domestic animal that results from the breeding of a male donkey ( Equus asinus ) to a female horse ( Equus caballus ). Usually, spermatogenesis in mules does not advance beyond spermatocytes. In the present study, we performed a comparative
and more accurate morphometric and functional investigation of the testis in donkeys and mules. Due to the smaller testis
size, lower seminiferous tubule volume density, and fewer germ cells, the total length of seminiferous tubules in mules was
significantly smaller than in donkeys. However, the percentage of seminiferous tubules containing germ cells (spermatogonia
and spermatocytes) in mules was approximately 95%. The total number of Sertoli cells per testis observed in donkeys and mules
was very similar. However, the total number of Leydig cells in mules was approximately 70% lower than in donkeys. At least
in part, this difference was probably related to the lower number of germ cells present in mule seminiferous tubules. Although
spermatogenesis in mules did not advance beyond secondary spermatocytes/newly formed round spermatids, germ cell associations
in the seminiferous epithelium and pachytene spermatocytes nuclear volume in donkeys and mules were similar. The duration
of spermatogenesis was estimated using intratesticular injections of tritiated thymidine. Each spermatogenic cycle in donkeys
lasted 10.5 days. A similar value was found in mules (â¼10.1 days). Considering that the entire spermatogenic process takes
approximately 4.5 cycles to be completed, its total duration in donkeys was estimated to last 47.2 days. The results found
for mules suggest that the mechanisms involved in the determination of testis structure and function are probably originated
from donkeys. Also, the data found for mules suggest that their seminiferous tubules are able to sustain complete spermatogenesis.
In this regard, this species is a potential model for transplants of germ cells originated from donkeys and horses or other
large animals. |
| doi_str_mv | 10.1095/biolreprod67.1.247 |
| format | Article |
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and more accurate morphometric and functional investigation of the testis in donkeys and mules. Due to the smaller testis
size, lower seminiferous tubule volume density, and fewer germ cells, the total length of seminiferous tubules in mules was
significantly smaller than in donkeys. However, the percentage of seminiferous tubules containing germ cells (spermatogonia
and spermatocytes) in mules was approximately 95%. The total number of Sertoli cells per testis observed in donkeys and mules
was very similar. However, the total number of Leydig cells in mules was approximately 70% lower than in donkeys. At least
in part, this difference was probably related to the lower number of germ cells present in mule seminiferous tubules. Although
spermatogenesis in mules did not advance beyond secondary spermatocytes/newly formed round spermatids, germ cell associations
in the seminiferous epithelium and pachytene spermatocytes nuclear volume in donkeys and mules were similar. The duration
of spermatogenesis was estimated using intratesticular injections of tritiated thymidine. Each spermatogenic cycle in donkeys
lasted 10.5 days. A similar value was found in mules (â¼10.1 days). Considering that the entire spermatogenic process takes
approximately 4.5 cycles to be completed, its total duration in donkeys was estimated to last 47.2 days. The results found
for mules suggest that the mechanisms involved in the determination of testis structure and function are probably originated
from donkeys. Also, the data found for mules suggest that their seminiferous tubules are able to sustain complete spermatogenesis.
In this regard, this species is a potential model for transplants of germ cells originated from donkeys and horses or other
large animals.</description><identifier>ISSN: 0006-3363</identifier><identifier>EISSN: 1529-7268</identifier><identifier>DOI: 10.1095/biolreprod67.1.247</identifier><identifier>PMID: 12080024</identifier><identifier>CODEN: BIREBV</identifier><language>eng</language><publisher>Madison, WI: Society for the Study of Reproduction</publisher><subject>Animals ; Apoptosis - physiology ; Biological and medical sciences ; Cell Count ; Cell Nucleus - physiology ; Cell Nucleus - ultrastructure ; Equidae - physiology ; Fundamental and applied biological sciences. Psychology ; Leydig Cells - physiology ; Male ; Mammalian male genital system ; Morphology. Physiology ; Seminiferous Epithelium - anatomy & histology ; Seminiferous Epithelium - drug effects ; Seminiferous Epithelium - physiology ; Seminiferous Tubules - anatomy & histology ; Seminiferous Tubules - drug effects ; Seminiferous Tubules - physiology ; Sertoli Cells - physiology ; Spermatocytes - physiology ; Spermatocytes - ultrastructure ; Spermatogenesis - physiology ; Testis - anatomy & histology ; Testis - drug effects ; Testis - physiology ; Thymidine - pharmacology ; Vertebrates: reproduction ; Y Chromosome - physiology</subject><ispartof>Biology of reproduction, 2002-07, Vol.67 (1), p.247-255</ispartof><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13757614$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12080024$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>NEVES, Elizabeth S</creatorcontrib><creatorcontrib>CHIARINI-GARCIA, Hélio</creatorcontrib><creatorcontrib>FRANCA, Luiz R</creatorcontrib><title>Comparative Testis Morphometry and Seminiferous Epithelium Cycle Length in Donkeys and Mules</title><title>Biology of reproduction</title><addtitle>Biol Reprod</addtitle><description>The mule ( Equus mulus mulus ) is a sterile hybrid domestic animal that results from the breeding of a male donkey ( Equus asinus ) to a female horse ( Equus caballus ). Usually, spermatogenesis in mules does not advance beyond spermatocytes. In the present study, we performed a comparative
and more accurate morphometric and functional investigation of the testis in donkeys and mules. Due to the smaller testis
size, lower seminiferous tubule volume density, and fewer germ cells, the total length of seminiferous tubules in mules was
significantly smaller than in donkeys. However, the percentage of seminiferous tubules containing germ cells (spermatogonia
and spermatocytes) in mules was approximately 95%. The total number of Sertoli cells per testis observed in donkeys and mules
was very similar. However, the total number of Leydig cells in mules was approximately 70% lower than in donkeys. At least
in part, this difference was probably related to the lower number of germ cells present in mule seminiferous tubules. Although
spermatogenesis in mules did not advance beyond secondary spermatocytes/newly formed round spermatids, germ cell associations
in the seminiferous epithelium and pachytene spermatocytes nuclear volume in donkeys and mules were similar. The duration
of spermatogenesis was estimated using intratesticular injections of tritiated thymidine. Each spermatogenic cycle in donkeys
lasted 10.5 days. A similar value was found in mules (â¼10.1 days). Considering that the entire spermatogenic process takes
approximately 4.5 cycles to be completed, its total duration in donkeys was estimated to last 47.2 days. The results found
for mules suggest that the mechanisms involved in the determination of testis structure and function are probably originated
from donkeys. Also, the data found for mules suggest that their seminiferous tubules are able to sustain complete spermatogenesis.
In this regard, this species is a potential model for transplants of germ cells originated from donkeys and horses or other
large animals.</description><subject>Animals</subject><subject>Apoptosis - physiology</subject><subject>Biological and medical sciences</subject><subject>Cell Count</subject><subject>Cell Nucleus - physiology</subject><subject>Cell Nucleus - ultrastructure</subject><subject>Equidae - physiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Leydig Cells - physiology</subject><subject>Male</subject><subject>Mammalian male genital system</subject><subject>Morphology. Physiology</subject><subject>Seminiferous Epithelium - anatomy & histology</subject><subject>Seminiferous Epithelium - drug effects</subject><subject>Seminiferous Epithelium - physiology</subject><subject>Seminiferous Tubules - anatomy & histology</subject><subject>Seminiferous Tubules - drug effects</subject><subject>Seminiferous Tubules - physiology</subject><subject>Sertoli Cells - physiology</subject><subject>Spermatocytes - physiology</subject><subject>Spermatocytes - ultrastructure</subject><subject>Spermatogenesis - physiology</subject><subject>Testis - anatomy & histology</subject><subject>Testis - drug effects</subject><subject>Testis - physiology</subject><subject>Thymidine - pharmacology</subject><subject>Vertebrates: reproduction</subject><subject>Y Chromosome - physiology</subject><issn>0006-3363</issn><issn>1529-7268</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpN0U1v2zAMBmBhWNFmbf_ADoMu680pJdmSfRyydCuQoIemtwKGLNOxNvljkr0g_35Cl6I98fKQIF8S8pnBkkGR3VZ2cB5HP9RSLdmSp-oDWbCMF4niMv9IFgAgEyGkuCCfQvgFwFLBxTm5YBxyAJ4uyPNq6Ebt9WT_It1hmGyg28GP7dDh5I9U9zV9xM72tkE_zIGuRzu16Ozc0dXROKQb7PdTS21Pvw_9bzyGl57t7DBckbNGu4DXp3pJnu7Wu9XPZPPw4371bZO0XKopKfIK8sZwlJkSrMqlrEA2AlPRAKQCMl6nxshasgpNjZEVRhgsFMvBcAbiktz8nxuz-DPHI8rOBoPO6R7jzmWEac5zHuGXE5yrDuty9LbT_li-5hHB1xPQwWjXeN0bG96cUJmS7J1r7b49WI9l6LRzcawoD4eDVCUr4z_EP1YFfrg</recordid><startdate>20020701</startdate><enddate>20020701</enddate><creator>NEVES, Elizabeth S</creator><creator>CHIARINI-GARCIA, Hélio</creator><creator>FRANCA, Luiz R</creator><general>Society for the Study of Reproduction</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20020701</creationdate><title>Comparative Testis Morphometry and Seminiferous Epithelium Cycle Length in Donkeys and Mules</title><author>NEVES, Elizabeth S ; CHIARINI-GARCIA, Hélio ; FRANCA, Luiz R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h267t-98b08fc2e65731b866b06f3e43f0043052d4cc6d61becdee659c3ce97180c2103</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Animals</topic><topic>Apoptosis - physiology</topic><topic>Biological and medical sciences</topic><topic>Cell Count</topic><topic>Cell Nucleus - physiology</topic><topic>Cell Nucleus - ultrastructure</topic><topic>Equidae - physiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Leydig Cells - physiology</topic><topic>Male</topic><topic>Mammalian male genital system</topic><topic>Morphology. Physiology</topic><topic>Seminiferous Epithelium - anatomy & histology</topic><topic>Seminiferous Epithelium - drug effects</topic><topic>Seminiferous Epithelium - physiology</topic><topic>Seminiferous Tubules - anatomy & histology</topic><topic>Seminiferous Tubules - drug effects</topic><topic>Seminiferous Tubules - physiology</topic><topic>Sertoli Cells - physiology</topic><topic>Spermatocytes - physiology</topic><topic>Spermatocytes - ultrastructure</topic><topic>Spermatogenesis - physiology</topic><topic>Testis - anatomy & histology</topic><topic>Testis - drug effects</topic><topic>Testis - physiology</topic><topic>Thymidine - pharmacology</topic><topic>Vertebrates: reproduction</topic><topic>Y Chromosome - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>NEVES, Elizabeth S</creatorcontrib><creatorcontrib>CHIARINI-GARCIA, Hélio</creatorcontrib><creatorcontrib>FRANCA, Luiz R</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Biology of reproduction</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>NEVES, Elizabeth S</au><au>CHIARINI-GARCIA, Hélio</au><au>FRANCA, Luiz R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparative Testis Morphometry and Seminiferous Epithelium Cycle Length in Donkeys and Mules</atitle><jtitle>Biology of reproduction</jtitle><addtitle>Biol Reprod</addtitle><date>2002-07-01</date><risdate>2002</risdate><volume>67</volume><issue>1</issue><spage>247</spage><epage>255</epage><pages>247-255</pages><issn>0006-3363</issn><eissn>1529-7268</eissn><coden>BIREBV</coden><abstract>The mule ( Equus mulus mulus ) is a sterile hybrid domestic animal that results from the breeding of a male donkey ( Equus asinus ) to a female horse ( Equus caballus ). Usually, spermatogenesis in mules does not advance beyond spermatocytes. In the present study, we performed a comparative
and more accurate morphometric and functional investigation of the testis in donkeys and mules. Due to the smaller testis
size, lower seminiferous tubule volume density, and fewer germ cells, the total length of seminiferous tubules in mules was
significantly smaller than in donkeys. However, the percentage of seminiferous tubules containing germ cells (spermatogonia
and spermatocytes) in mules was approximately 95%. The total number of Sertoli cells per testis observed in donkeys and mules
was very similar. However, the total number of Leydig cells in mules was approximately 70% lower than in donkeys. At least
in part, this difference was probably related to the lower number of germ cells present in mule seminiferous tubules. Although
spermatogenesis in mules did not advance beyond secondary spermatocytes/newly formed round spermatids, germ cell associations
in the seminiferous epithelium and pachytene spermatocytes nuclear volume in donkeys and mules were similar. The duration
of spermatogenesis was estimated using intratesticular injections of tritiated thymidine. Each spermatogenic cycle in donkeys
lasted 10.5 days. A similar value was found in mules (â¼10.1 days). Considering that the entire spermatogenic process takes
approximately 4.5 cycles to be completed, its total duration in donkeys was estimated to last 47.2 days. The results found
for mules suggest that the mechanisms involved in the determination of testis structure and function are probably originated
from donkeys. Also, the data found for mules suggest that their seminiferous tubules are able to sustain complete spermatogenesis.
In this regard, this species is a potential model for transplants of germ cells originated from donkeys and horses or other
large animals.</abstract><cop>Madison, WI</cop><pub>Society for the Study of Reproduction</pub><pmid>12080024</pmid><doi>10.1095/biolreprod67.1.247</doi><tpages>9</tpages></addata></record> |
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| ispartof | Biology of reproduction, 2002-07, Vol.67 (1), p.247-255 |
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| source | MEDLINE; BioOne Complete; Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals |
| subjects | Animals Apoptosis - physiology Biological and medical sciences Cell Count Cell Nucleus - physiology Cell Nucleus - ultrastructure Equidae - physiology Fundamental and applied biological sciences. Psychology Leydig Cells - physiology Male Mammalian male genital system Morphology. Physiology Seminiferous Epithelium - anatomy & histology Seminiferous Epithelium - drug effects Seminiferous Epithelium - physiology Seminiferous Tubules - anatomy & histology Seminiferous Tubules - drug effects Seminiferous Tubules - physiology Sertoli Cells - physiology Spermatocytes - physiology Spermatocytes - ultrastructure Spermatogenesis - physiology Testis - anatomy & histology Testis - drug effects Testis - physiology Thymidine - pharmacology Vertebrates: reproduction Y Chromosome - physiology |
| title | Comparative Testis Morphometry and Seminiferous Epithelium Cycle Length in Donkeys and Mules |
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