Differential Expression of Microsomal Prostaglandin E Synthase at Implantation Sites and in Decidual Cells of Mouse Uterus
Prostaglandin E 2 (PGE 2 ) is considered important for blastocyst spacing, implantation, and decidualization in the rodent uterus. PGE synthase (PGES) catalyzes the isomerization of PGH 2 to PGE 2 . There are two isoforms of PGES, microsomal PGES (mPGES) and cytosolic PGES (cPGES). However, the expr...
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| Veröffentlicht in: | Biology of reproduction 2002-07, Vol.67 (1), p.351-358 |
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| creator | HUA NI TONG SUN DING, Nai-Zheng MA, Xing-Hong YANG, Zeng-Ming |
| description | Prostaglandin E 2 (PGE 2 ) is considered important for blastocyst spacing, implantation, and decidualization in the rodent uterus. PGE synthase (PGES)
catalyzes the isomerization of PGH 2 to PGE 2 . There are two isoforms of PGES, microsomal PGES (mPGES) and cytosolic PGES (cPGES). However, the expression and regulation
of mPGES in the mammalian uterus during early pregnancy are still unknown. The aim of this study was to investigate the differential
expression of mPGES in mouse uterus during early pregnancy and its regulation under different conditions by in situ hybridization
and immunohistochemistry. Microsomal PGES expression in the preimplantation mouse embryos was also performed by reverse transcription
polymerase chain reaction (RT-PCR). Expression of mPGES mRNA and protein was at a basal level in the luminal epithelium from
Day 1 to Day 4 of pregnancy. However, mPGES mRNA and protein were highly expressed in the stroma immediately surrounding the
blastocyst but not in the luminal epithelium on Day 5 of pregnancy. Microsomal PGES mRNA and protein were not detected in
the pseudopregnant uterus from Day 1 to Day 5. During delayed implantation, mPGES mRNA and protein were also not detected
in the uterus. Once delayed implantation was terminated by estrogen treatment and embryo implantation initiated, both mPGES
mRNA and protein were induced to express in the stroma immediately surrounding the blastocyst, which was similar to the expression
pattern on Day 5 of pregnancy. From Day 6 to Day 8 of pregnancy, the signals for mPGES mRNA and protein were strongly detected
in the decidualized cells. Microsomal PGES mRNA and protein were also highly expressed in the artificially decidualized cells
but not in the control horn. Microsomal PGES mRNA was detected in the oocytes and all the stages of preimplantation embryos.
The strong mPGES expression in the implantation site and decidual cells suggests that mPGES might play an important role during
implantation and more importantly in decidualization. |
| doi_str_mv | 10.1095/biolreprod67.1.351 |
| format | Article |
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catalyzes the isomerization of PGH 2 to PGE 2 . There are two isoforms of PGES, microsomal PGES (mPGES) and cytosolic PGES (cPGES). However, the expression and regulation
of mPGES in the mammalian uterus during early pregnancy are still unknown. The aim of this study was to investigate the differential
expression of mPGES in mouse uterus during early pregnancy and its regulation under different conditions by in situ hybridization
and immunohistochemistry. Microsomal PGES expression in the preimplantation mouse embryos was also performed by reverse transcription
polymerase chain reaction (RT-PCR). Expression of mPGES mRNA and protein was at a basal level in the luminal epithelium from
Day 1 to Day 4 of pregnancy. However, mPGES mRNA and protein were highly expressed in the stroma immediately surrounding the
blastocyst but not in the luminal epithelium on Day 5 of pregnancy. Microsomal PGES mRNA and protein were not detected in
the pseudopregnant uterus from Day 1 to Day 5. During delayed implantation, mPGES mRNA and protein were also not detected
in the uterus. Once delayed implantation was terminated by estrogen treatment and embryo implantation initiated, both mPGES
mRNA and protein were induced to express in the stroma immediately surrounding the blastocyst, which was similar to the expression
pattern on Day 5 of pregnancy. From Day 6 to Day 8 of pregnancy, the signals for mPGES mRNA and protein were strongly detected
in the decidualized cells. Microsomal PGES mRNA and protein were also highly expressed in the artificially decidualized cells
but not in the control horn. Microsomal PGES mRNA was detected in the oocytes and all the stages of preimplantation embryos.
The strong mPGES expression in the implantation site and decidual cells suggests that mPGES might play an important role during
implantation and more importantly in decidualization.</description><identifier>ISSN: 0006-3363</identifier><identifier>EISSN: 1529-7268</identifier><identifier>DOI: 10.1095/biolreprod67.1.351</identifier><identifier>PMID: 12080039</identifier><identifier>CODEN: BIREBV</identifier><language>eng</language><publisher>Madison, WI: Society for the Study of Reproduction</publisher><subject>Animals ; Biological and medical sciences ; Blastocyst - metabolism ; Decidua - cytology ; Decidua - enzymology ; Early stages. Segmentation. Gastrulation. Neurulation ; Embryo Implantation - physiology ; Embryology: invertebrates and vertebrates. Teratology ; Female ; Fundamental and applied biological sciences. Psychology ; Immunohistochemistry ; In Situ Hybridization ; Intramolecular Oxidoreductases - biosynthesis ; Mice ; Oocytes - metabolism ; Ovariectomy ; Pregnancy ; Pregnancy, Animal - physiology ; Prostaglandin-E Synthases ; Pseudopregnancy - enzymology ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - biosynthesis ; RNA, Messenger - genetics ; Uterus - cytology ; Uterus - enzymology</subject><ispartof>Biology of reproduction, 2002-07, Vol.67 (1), p.351-358</ispartof><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13757629$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12080039$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>HUA NI</creatorcontrib><creatorcontrib>TONG SUN</creatorcontrib><creatorcontrib>DING, Nai-Zheng</creatorcontrib><creatorcontrib>MA, Xing-Hong</creatorcontrib><creatorcontrib>YANG, Zeng-Ming</creatorcontrib><title>Differential Expression of Microsomal Prostaglandin E Synthase at Implantation Sites and in Decidual Cells of Mouse Uterus</title><title>Biology of reproduction</title><addtitle>Biol Reprod</addtitle><description>Prostaglandin E 2 (PGE 2 ) is considered important for blastocyst spacing, implantation, and decidualization in the rodent uterus. PGE synthase (PGES)
catalyzes the isomerization of PGH 2 to PGE 2 . There are two isoforms of PGES, microsomal PGES (mPGES) and cytosolic PGES (cPGES). However, the expression and regulation
of mPGES in the mammalian uterus during early pregnancy are still unknown. The aim of this study was to investigate the differential
expression of mPGES in mouse uterus during early pregnancy and its regulation under different conditions by in situ hybridization
and immunohistochemistry. Microsomal PGES expression in the preimplantation mouse embryos was also performed by reverse transcription
polymerase chain reaction (RT-PCR). Expression of mPGES mRNA and protein was at a basal level in the luminal epithelium from
Day 1 to Day 4 of pregnancy. However, mPGES mRNA and protein were highly expressed in the stroma immediately surrounding the
blastocyst but not in the luminal epithelium on Day 5 of pregnancy. Microsomal PGES mRNA and protein were not detected in
the pseudopregnant uterus from Day 1 to Day 5. During delayed implantation, mPGES mRNA and protein were also not detected
in the uterus. Once delayed implantation was terminated by estrogen treatment and embryo implantation initiated, both mPGES
mRNA and protein were induced to express in the stroma immediately surrounding the blastocyst, which was similar to the expression
pattern on Day 5 of pregnancy. From Day 6 to Day 8 of pregnancy, the signals for mPGES mRNA and protein were strongly detected
in the decidualized cells. Microsomal PGES mRNA and protein were also highly expressed in the artificially decidualized cells
but not in the control horn. Microsomal PGES mRNA was detected in the oocytes and all the stages of preimplantation embryos.
The strong mPGES expression in the implantation site and decidual cells suggests that mPGES might play an important role during
implantation and more importantly in decidualization.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Blastocyst - metabolism</subject><subject>Decidua - cytology</subject><subject>Decidua - enzymology</subject><subject>Early stages. Segmentation. Gastrulation. Neurulation</subject><subject>Embryo Implantation - physiology</subject><subject>Embryology: invertebrates and vertebrates. Teratology</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Immunohistochemistry</subject><subject>In Situ Hybridization</subject><subject>Intramolecular Oxidoreductases - biosynthesis</subject><subject>Mice</subject><subject>Oocytes - metabolism</subject><subject>Ovariectomy</subject><subject>Pregnancy</subject><subject>Pregnancy, Animal - physiology</subject><subject>Prostaglandin-E Synthases</subject><subject>Pseudopregnancy - enzymology</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - biosynthesis</subject><subject>RNA, Messenger - genetics</subject><subject>Uterus - cytology</subject><subject>Uterus - enzymology</subject><issn>0006-3363</issn><issn>1529-7268</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkU9v1DAQxS1ERbeFL8AB-QK3LGM7tuMj2i60UlErtT1Hk2TSNcqfxXa0LZ8eA4vgZMvze09vnhl7K2AtwOmPjZ-HQPswd8auxVpp8YKthJausNJUL9kKAEyhlFGn7CzGbwCiVFK9YqdCQgWg3Ir9uPB9T4Gm5HHg26d9oBj9PPG55199G-Y4j3lwmy8JHwecOj_xLb97ntIOI3FM_Grc5_eE6ZfszieKPGM8cxfU-m7J8g0NQ_xtOS9Z9JAoLPE1O-lxiPTmeJ6zh8_b-81lcX3z5Wrz6brYSWNToaHrHDYSGlm2VIEA0xtC7AwZ1ZR5C9nKEnXrNIG1lbZgHGjrSouu1L06Zx_--Oamvi8UUz362OZEOFGOU1tRlaV1LoPvjuDSjNTV--BHDM_137Yy8P4IYGxx6ANOrY__OGW1NfI_bucfdwcfqI65xCHbqvpwOBhbizr_lvoJcnaJAA</recordid><startdate>20020701</startdate><enddate>20020701</enddate><creator>HUA NI</creator><creator>TONG SUN</creator><creator>DING, Nai-Zheng</creator><creator>MA, Xing-Hong</creator><creator>YANG, Zeng-Ming</creator><general>Society for the Study of Reproduction</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20020701</creationdate><title>Differential Expression of Microsomal Prostaglandin E Synthase at Implantation Sites and in Decidual Cells of Mouse Uterus</title><author>HUA NI ; TONG SUN ; DING, Nai-Zheng ; MA, Xing-Hong ; YANG, Zeng-Ming</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h267t-50dd9ab20b24ce80106f6eaad6e63b40392c24a5c95e077857069057947a945f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Blastocyst - metabolism</topic><topic>Decidua - cytology</topic><topic>Decidua - enzymology</topic><topic>Early stages. Segmentation. Gastrulation. Neurulation</topic><topic>Embryo Implantation - physiology</topic><topic>Embryology: invertebrates and vertebrates. Teratology</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Immunohistochemistry</topic><topic>In Situ Hybridization</topic><topic>Intramolecular Oxidoreductases - biosynthesis</topic><topic>Mice</topic><topic>Oocytes - metabolism</topic><topic>Ovariectomy</topic><topic>Pregnancy</topic><topic>Pregnancy, Animal - physiology</topic><topic>Prostaglandin-E Synthases</topic><topic>Pseudopregnancy - enzymology</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - biosynthesis</topic><topic>RNA, Messenger - genetics</topic><topic>Uterus - cytology</topic><topic>Uterus - enzymology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>HUA NI</creatorcontrib><creatorcontrib>TONG SUN</creatorcontrib><creatorcontrib>DING, Nai-Zheng</creatorcontrib><creatorcontrib>MA, Xing-Hong</creatorcontrib><creatorcontrib>YANG, Zeng-Ming</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Biology of reproduction</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>HUA NI</au><au>TONG SUN</au><au>DING, Nai-Zheng</au><au>MA, Xing-Hong</au><au>YANG, Zeng-Ming</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differential Expression of Microsomal Prostaglandin E Synthase at Implantation Sites and in Decidual Cells of Mouse Uterus</atitle><jtitle>Biology of reproduction</jtitle><addtitle>Biol Reprod</addtitle><date>2002-07-01</date><risdate>2002</risdate><volume>67</volume><issue>1</issue><spage>351</spage><epage>358</epage><pages>351-358</pages><issn>0006-3363</issn><eissn>1529-7268</eissn><coden>BIREBV</coden><abstract>Prostaglandin E 2 (PGE 2 ) is considered important for blastocyst spacing, implantation, and decidualization in the rodent uterus. PGE synthase (PGES)
catalyzes the isomerization of PGH 2 to PGE 2 . There are two isoforms of PGES, microsomal PGES (mPGES) and cytosolic PGES (cPGES). However, the expression and regulation
of mPGES in the mammalian uterus during early pregnancy are still unknown. The aim of this study was to investigate the differential
expression of mPGES in mouse uterus during early pregnancy and its regulation under different conditions by in situ hybridization
and immunohistochemistry. Microsomal PGES expression in the preimplantation mouse embryos was also performed by reverse transcription
polymerase chain reaction (RT-PCR). Expression of mPGES mRNA and protein was at a basal level in the luminal epithelium from
Day 1 to Day 4 of pregnancy. However, mPGES mRNA and protein were highly expressed in the stroma immediately surrounding the
blastocyst but not in the luminal epithelium on Day 5 of pregnancy. Microsomal PGES mRNA and protein were not detected in
the pseudopregnant uterus from Day 1 to Day 5. During delayed implantation, mPGES mRNA and protein were also not detected
in the uterus. Once delayed implantation was terminated by estrogen treatment and embryo implantation initiated, both mPGES
mRNA and protein were induced to express in the stroma immediately surrounding the blastocyst, which was similar to the expression
pattern on Day 5 of pregnancy. From Day 6 to Day 8 of pregnancy, the signals for mPGES mRNA and protein were strongly detected
in the decidualized cells. Microsomal PGES mRNA and protein were also highly expressed in the artificially decidualized cells
but not in the control horn. Microsomal PGES mRNA was detected in the oocytes and all the stages of preimplantation embryos.
The strong mPGES expression in the implantation site and decidual cells suggests that mPGES might play an important role during
implantation and more importantly in decidualization.</abstract><cop>Madison, WI</cop><pub>Society for the Study of Reproduction</pub><pmid>12080039</pmid><doi>10.1095/biolreprod67.1.351</doi><tpages>8</tpages></addata></record> |
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| ispartof | Biology of reproduction, 2002-07, Vol.67 (1), p.351-358 |
| issn | 0006-3363 1529-7268 |
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| recordid | cdi_proquest_miscellaneous_71844799 |
| source | MEDLINE; BioOne Complete; Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals |
| subjects | Animals Biological and medical sciences Blastocyst - metabolism Decidua - cytology Decidua - enzymology Early stages. Segmentation. Gastrulation. Neurulation Embryo Implantation - physiology Embryology: invertebrates and vertebrates. Teratology Female Fundamental and applied biological sciences. Psychology Immunohistochemistry In Situ Hybridization Intramolecular Oxidoreductases - biosynthesis Mice Oocytes - metabolism Ovariectomy Pregnancy Pregnancy, Animal - physiology Prostaglandin-E Synthases Pseudopregnancy - enzymology Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - biosynthesis RNA, Messenger - genetics Uterus - cytology Uterus - enzymology |
| title | Differential Expression of Microsomal Prostaglandin E Synthase at Implantation Sites and in Decidual Cells of Mouse Uterus |
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