Cryptosporidium andersoni from a Danish cattle herd: identification and preliminary characterisation
In November 1997, Cryptosporidium andersoni, for the first time, was isolated from a Danish heifer. The isolate was characterised morphologically, molecularly, and furthermore inoculated into mice and one calf. Data on the distribution of cryptosporidia in the herd of origin were obtained at two sep...
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| Veröffentlicht in: | Veterinary parasitology 2002-07, Vol.107 (1), p.37-49 |
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| creator | Enemark, H.L Ahrens, P Lowery, C.J Thamsborg, S.M Enemark, J.M.D Bille-Hansen, V Lind, P |
| description | In November 1997,
Cryptosporidium andersoni, for the first time, was isolated from a Danish heifer. The isolate was characterised morphologically, molecularly, and furthermore inoculated into mice and one calf. Data on the distribution of cryptosporidia in the herd of origin were obtained at two separate visits in December 1997 and April 1998.
C. andersoni was detected in 27 (19.0%) of 142 cattle examined at the first visit, whereas
C. parvum was found in six (4.2%). At the following visit 42 (28.0%) of 150 cattle excreted
C. andersoni, while 25 (16.7%) were positive for
C. parvum. Oocysts of the Danish
C. andersoni isolate were ovoid,
7.3
(6.5–8.0)×5.7
(5.0–7.0)
μm
2 (
n=25), with smooth, colourless, single layer oocyst wall and distinct oocyst residuum. The length to width ratio was 1.27 (1.14–1.40,
n=25). The identification was verified by sequencing of a 246
bp fragment of the rDNA, which was identical to
Cryptosporidium muris, the calf genotype (AF093496). The Danish
C. andersoni isolate was not transmissible to mice, whereas oocysts were detected in the faeces of one experimentally infected calf from 25 days post-infection (DPI) and shed intermittently at low numbers until 165 DPI, the day of euthanasia. No macroscopic or microscopic changes that could be attributed to infection with
C. andersoni were seen in the gastro-intestinal tract of the experimentally infected calf following necropsy and histological examination. This is to our knowledge the first report of
C. andersoni in Scandinavia. |
| doi_str_mv | 10.1016/S0304-4017(02)00083-3 |
| format | Article |
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Cryptosporidium andersoni, for the first time, was isolated from a Danish heifer. The isolate was characterised morphologically, molecularly, and furthermore inoculated into mice and one calf. Data on the distribution of cryptosporidia in the herd of origin were obtained at two separate visits in December 1997 and April 1998.
C. andersoni was detected in 27 (19.0%) of 142 cattle examined at the first visit, whereas
C. parvum was found in six (4.2%). At the following visit 42 (28.0%) of 150 cattle excreted
C. andersoni, while 25 (16.7%) were positive for
C. parvum. Oocysts of the Danish
C. andersoni isolate were ovoid,
7.3
(6.5–8.0)×5.7
(5.0–7.0)
μm
2 (
n=25), with smooth, colourless, single layer oocyst wall and distinct oocyst residuum. The length to width ratio was 1.27 (1.14–1.40,
n=25). The identification was verified by sequencing of a 246
bp fragment of the rDNA, which was identical to
Cryptosporidium muris, the calf genotype (AF093496). The Danish
C. andersoni isolate was not transmissible to mice, whereas oocysts were detected in the faeces of one experimentally infected calf from 25 days post-infection (DPI) and shed intermittently at low numbers until 165 DPI, the day of euthanasia. No macroscopic or microscopic changes that could be attributed to infection with
C. andersoni were seen in the gastro-intestinal tract of the experimentally infected calf following necropsy and histological examination. This is to our knowledge the first report of
C. andersoni in Scandinavia.</description><identifier>ISSN: 0304-4017</identifier><identifier>EISSN: 1873-2550</identifier><identifier>DOI: 10.1016/S0304-4017(02)00083-3</identifier><identifier>PMID: 12072212</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Base Sequence ; Cattle ; Cattle Diseases - epidemiology ; Cattle Diseases - parasitology ; Cattle Diseases - transmission ; Cattle-protozoa ; Cryptosporidiosis - epidemiology ; Cryptosporidiosis - parasitology ; Cryptosporidiosis - transmission ; Cryptosporidiosis - veterinary ; Cryptosporidium - genetics ; Cryptosporidium - isolation & purification ; Cryptosporidium andersoni ; Cryptosporidium parvum ; Cryptosporidium parvum - genetics ; Cryptosporidium parvum - isolation & purification ; Denmark - epidemiology ; DNA, Ribosomal - chemistry ; DNA, Ribosomal - genetics ; Experimental infection ; Feces - parasitology ; Female ; Herd prevalence ; Male ; Mice ; Molecular characterisation ; Molecular Sequence Data ; Polymerase Chain Reaction - veterinary ; Polymorphism, Restriction Fragment Length ; Prevalence ; RNA, Ribosomal, 18S - genetics</subject><ispartof>Veterinary parasitology, 2002-07, Vol.107 (1), p.37-49</ispartof><rights>2002 Elsevier Science B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c392t-bbcdd12bdf489f0581c74e5441c07a5141fd3b76cc4d5c8d73aa5762a653646b3</citedby><cites>FETCH-LOGICAL-c392t-bbcdd12bdf489f0581c74e5441c07a5141fd3b76cc4d5c8d73aa5762a653646b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0304-4017(02)00083-3$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27922,27923,45993</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12072212$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Enemark, H.L</creatorcontrib><creatorcontrib>Ahrens, P</creatorcontrib><creatorcontrib>Lowery, C.J</creatorcontrib><creatorcontrib>Thamsborg, S.M</creatorcontrib><creatorcontrib>Enemark, J.M.D</creatorcontrib><creatorcontrib>Bille-Hansen, V</creatorcontrib><creatorcontrib>Lind, P</creatorcontrib><title>Cryptosporidium andersoni from a Danish cattle herd: identification and preliminary characterisation</title><title>Veterinary parasitology</title><addtitle>Vet Parasitol</addtitle><description>In November 1997,
Cryptosporidium andersoni, for the first time, was isolated from a Danish heifer. The isolate was characterised morphologically, molecularly, and furthermore inoculated into mice and one calf. Data on the distribution of cryptosporidia in the herd of origin were obtained at two separate visits in December 1997 and April 1998.
C. andersoni was detected in 27 (19.0%) of 142 cattle examined at the first visit, whereas
C. parvum was found in six (4.2%). At the following visit 42 (28.0%) of 150 cattle excreted
C. andersoni, while 25 (16.7%) were positive for
C. parvum. Oocysts of the Danish
C. andersoni isolate were ovoid,
7.3
(6.5–8.0)×5.7
(5.0–7.0)
μm
2 (
n=25), with smooth, colourless, single layer oocyst wall and distinct oocyst residuum. The length to width ratio was 1.27 (1.14–1.40,
n=25). The identification was verified by sequencing of a 246
bp fragment of the rDNA, which was identical to
Cryptosporidium muris, the calf genotype (AF093496). The Danish
C. andersoni isolate was not transmissible to mice, whereas oocysts were detected in the faeces of one experimentally infected calf from 25 days post-infection (DPI) and shed intermittently at low numbers until 165 DPI, the day of euthanasia. No macroscopic or microscopic changes that could be attributed to infection with
C. andersoni were seen in the gastro-intestinal tract of the experimentally infected calf following necropsy and histological examination. This is to our knowledge the first report of
C. andersoni in Scandinavia.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Cattle</subject><subject>Cattle Diseases - epidemiology</subject><subject>Cattle Diseases - parasitology</subject><subject>Cattle Diseases - transmission</subject><subject>Cattle-protozoa</subject><subject>Cryptosporidiosis - epidemiology</subject><subject>Cryptosporidiosis - parasitology</subject><subject>Cryptosporidiosis - transmission</subject><subject>Cryptosporidiosis - veterinary</subject><subject>Cryptosporidium - genetics</subject><subject>Cryptosporidium - isolation & purification</subject><subject>Cryptosporidium andersoni</subject><subject>Cryptosporidium parvum</subject><subject>Cryptosporidium parvum - genetics</subject><subject>Cryptosporidium parvum - isolation & purification</subject><subject>Denmark - epidemiology</subject><subject>DNA, Ribosomal - chemistry</subject><subject>DNA, Ribosomal - genetics</subject><subject>Experimental infection</subject><subject>Feces - parasitology</subject><subject>Female</subject><subject>Herd prevalence</subject><subject>Male</subject><subject>Mice</subject><subject>Molecular characterisation</subject><subject>Molecular Sequence Data</subject><subject>Polymerase Chain Reaction - veterinary</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>Prevalence</subject><subject>RNA, Ribosomal, 18S - genetics</subject><issn>0304-4017</issn><issn>1873-2550</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1P3DAQhq0KVBban0CVEyqHwPgrDr2gamkLEhIH2rPl2BPtoCRO7SzS_nuyH2qPnEYzet4Zzfsyds7higOvrp9BgioVcPMVxCUA1LKUH9iC10aWQms4Yot_yAk7zfllhhRU5iM74QKMEFwsWFimzTjFPMZEgdZ94YaAKceBijbFuS3u3EB5VXg3TR0WK0zhW0EBh4lamocUh62mGBN21NPg0qbwK5ecnzBR3gGf2HHruoyfD_WM_fn54_fyvnx8-vWw_P5YenkjprJpfAhcNKFV9U0LuubeKNRKcQ_Gaa54G2RjKu9V0L4ORjqnTSVcpWWlqkaesYv93jHFv2vMk-0pe-w6N2BcZ2t4rYSu4V1w5mqASs6g3oM-xZwTtnZM1M8_Wg52m4Pd5WC3JlsQdpeD3eq-HA6smx7Df9XB-Bm43QM4-_FKmGz2hIPHQAn9ZEOkd068AYflmPA</recordid><startdate>20020729</startdate><enddate>20020729</enddate><creator>Enemark, H.L</creator><creator>Ahrens, P</creator><creator>Lowery, C.J</creator><creator>Thamsborg, S.M</creator><creator>Enemark, J.M.D</creator><creator>Bille-Hansen, V</creator><creator>Lind, P</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>20020729</creationdate><title>Cryptosporidium andersoni from a Danish cattle herd: identification and preliminary characterisation</title><author>Enemark, H.L ; Ahrens, P ; Lowery, C.J ; Thamsborg, S.M ; Enemark, J.M.D ; Bille-Hansen, V ; Lind, P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c392t-bbcdd12bdf489f0581c74e5441c07a5141fd3b76cc4d5c8d73aa5762a653646b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Animals</topic><topic>Base Sequence</topic><topic>Cattle</topic><topic>Cattle Diseases - epidemiology</topic><topic>Cattle Diseases - parasitology</topic><topic>Cattle Diseases - transmission</topic><topic>Cattle-protozoa</topic><topic>Cryptosporidiosis - epidemiology</topic><topic>Cryptosporidiosis - parasitology</topic><topic>Cryptosporidiosis - transmission</topic><topic>Cryptosporidiosis - veterinary</topic><topic>Cryptosporidium - genetics</topic><topic>Cryptosporidium - isolation & purification</topic><topic>Cryptosporidium andersoni</topic><topic>Cryptosporidium parvum</topic><topic>Cryptosporidium parvum - genetics</topic><topic>Cryptosporidium parvum - isolation & purification</topic><topic>Denmark - epidemiology</topic><topic>DNA, Ribosomal - chemistry</topic><topic>DNA, Ribosomal - genetics</topic><topic>Experimental infection</topic><topic>Feces - parasitology</topic><topic>Female</topic><topic>Herd prevalence</topic><topic>Male</topic><topic>Mice</topic><topic>Molecular characterisation</topic><topic>Molecular Sequence Data</topic><topic>Polymerase Chain Reaction - veterinary</topic><topic>Polymorphism, Restriction Fragment Length</topic><topic>Prevalence</topic><topic>RNA, Ribosomal, 18S - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Enemark, H.L</creatorcontrib><creatorcontrib>Ahrens, P</creatorcontrib><creatorcontrib>Lowery, C.J</creatorcontrib><creatorcontrib>Thamsborg, S.M</creatorcontrib><creatorcontrib>Enemark, J.M.D</creatorcontrib><creatorcontrib>Bille-Hansen, V</creatorcontrib><creatorcontrib>Lind, P</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Enemark, H.L</au><au>Ahrens, P</au><au>Lowery, C.J</au><au>Thamsborg, S.M</au><au>Enemark, J.M.D</au><au>Bille-Hansen, V</au><au>Lind, P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cryptosporidium andersoni from a Danish cattle herd: identification and preliminary characterisation</atitle><jtitle>Veterinary parasitology</jtitle><addtitle>Vet Parasitol</addtitle><date>2002-07-29</date><risdate>2002</risdate><volume>107</volume><issue>1</issue><spage>37</spage><epage>49</epage><pages>37-49</pages><issn>0304-4017</issn><eissn>1873-2550</eissn><abstract>In November 1997,
Cryptosporidium andersoni, for the first time, was isolated from a Danish heifer. The isolate was characterised morphologically, molecularly, and furthermore inoculated into mice and one calf. Data on the distribution of cryptosporidia in the herd of origin were obtained at two separate visits in December 1997 and April 1998.
C. andersoni was detected in 27 (19.0%) of 142 cattle examined at the first visit, whereas
C. parvum was found in six (4.2%). At the following visit 42 (28.0%) of 150 cattle excreted
C. andersoni, while 25 (16.7%) were positive for
C. parvum. Oocysts of the Danish
C. andersoni isolate were ovoid,
7.3
(6.5–8.0)×5.7
(5.0–7.0)
μm
2 (
n=25), with smooth, colourless, single layer oocyst wall and distinct oocyst residuum. The length to width ratio was 1.27 (1.14–1.40,
n=25). The identification was verified by sequencing of a 246
bp fragment of the rDNA, which was identical to
Cryptosporidium muris, the calf genotype (AF093496). The Danish
C. andersoni isolate was not transmissible to mice, whereas oocysts were detected in the faeces of one experimentally infected calf from 25 days post-infection (DPI) and shed intermittently at low numbers until 165 DPI, the day of euthanasia. No macroscopic or microscopic changes that could be attributed to infection with
C. andersoni were seen in the gastro-intestinal tract of the experimentally infected calf following necropsy and histological examination. This is to our knowledge the first report of
C. andersoni in Scandinavia.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>12072212</pmid><doi>10.1016/S0304-4017(02)00083-3</doi><tpages>13</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0304-4017 |
| ispartof | Veterinary parasitology, 2002-07, Vol.107 (1), p.37-49 |
| issn | 0304-4017 1873-2550 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_71842580 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals Base Sequence Cattle Cattle Diseases - epidemiology Cattle Diseases - parasitology Cattle Diseases - transmission Cattle-protozoa Cryptosporidiosis - epidemiology Cryptosporidiosis - parasitology Cryptosporidiosis - transmission Cryptosporidiosis - veterinary Cryptosporidium - genetics Cryptosporidium - isolation & purification Cryptosporidium andersoni Cryptosporidium parvum Cryptosporidium parvum - genetics Cryptosporidium parvum - isolation & purification Denmark - epidemiology DNA, Ribosomal - chemistry DNA, Ribosomal - genetics Experimental infection Feces - parasitology Female Herd prevalence Male Mice Molecular characterisation Molecular Sequence Data Polymerase Chain Reaction - veterinary Polymorphism, Restriction Fragment Length Prevalence RNA, Ribosomal, 18S - genetics |
| title | Cryptosporidium andersoni from a Danish cattle herd: identification and preliminary characterisation |
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