Preliminary Study on Puffer Fish ProteomeSpecies Identification of Puffer Fish by Two-Dimensional Electrophoresis
The aims of this work were to determine the differential characterization of the urea soluble protein components of puffer fish species and to establish a preliminary proteomic database using an immobilized pH gradient two-dimensional electrophoresis (2DE) technique. The puffer fish muscle proteins...
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| Veröffentlicht in: | Journal of agricultural and food chemistry 2004-04, Vol.52 (8), p.2236-2241 |
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| creator | Chen, Tai-Yuan Shiau, Chyuan-Yuan Wei, Cheng-I Hwang, Deng-Fwu |
| description | The aims of this work were to determine the differential characterization of the urea soluble protein components of puffer fish species and to establish a preliminary proteomic database using an immobilized pH gradient two-dimensional electrophoresis (2DE) technique. The puffer fish muscle proteins resolved into 171−260 spots in the 2DE gels, with a pI range of 3−10 and molecular mass range of 7.4−205.0 kDa, following Comassie blue staining. Puffer fish muscle proteins fell in the region with pI values of 3.5−7.0, and molecular masses of 7.4−45.0 kDa were well-resolved and were good for species comparison. The more acidic proteins of lower molecular masses showed species specific characteristics. Therefore, the species of puffer fish can be differentiated from the comparison of the characteristic 2DE protein patterns. Keywords: Two-dimensional electrophoresis; puffer fish; species identification; proteome; protein |
| doi_str_mv | 10.1021/jf035033n |
| format | Article |
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Psychology ; gel electrophoresis ; Hydrogen-Ion Concentration ; Isoelectric Point ; Lagocephalus ; marine fish ; Molecular Weight ; Muscle Proteins - chemistry ; Muscle Proteins - isolation & purification ; product authenticity ; protein composition ; proteome ; Proteome - isolation & purification ; proteomics ; Solubility ; Species Specificity ; Tetraodontidae ; Tetraodontiformes - classification ; Urea ; urea soluble proteins</subject><ispartof>Journal of agricultural and food chemistry, 2004-04, Vol.52 (8), p.2236-2241</ispartof><rights>Copyright © 2004 American Chemical Society</rights><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/jf035033n$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/jf035033n$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>315,782,786,27083,27931,27932,56745,56795</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15916093$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15080627$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, Tai-Yuan</creatorcontrib><creatorcontrib>Shiau, Chyuan-Yuan</creatorcontrib><creatorcontrib>Wei, Cheng-I</creatorcontrib><creatorcontrib>Hwang, Deng-Fwu</creatorcontrib><title>Preliminary Study on Puffer Fish ProteomeSpecies Identification of Puffer Fish by Two-Dimensional Electrophoresis</title><title>Journal of agricultural and food chemistry</title><addtitle>J. Agric. Food Chem</addtitle><description>The aims of this work were to determine the differential characterization of the urea soluble protein components of puffer fish species and to establish a preliminary proteomic database using an immobilized pH gradient two-dimensional electrophoresis (2DE) technique. The puffer fish muscle proteins resolved into 171−260 spots in the 2DE gels, with a pI range of 3−10 and molecular mass range of 7.4−205.0 kDa, following Comassie blue staining. Puffer fish muscle proteins fell in the region with pI values of 3.5−7.0, and molecular masses of 7.4−45.0 kDa were well-resolved and were good for species comparison. The more acidic proteins of lower molecular masses showed species specific characteristics. Therefore, the species of puffer fish can be differentiated from the comparison of the characteristic 2DE protein patterns. 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Psychology</subject><subject>gel electrophoresis</subject><subject>Hydrogen-Ion Concentration</subject><subject>Isoelectric Point</subject><subject>Lagocephalus</subject><subject>marine fish</subject><subject>Molecular Weight</subject><subject>Muscle Proteins - chemistry</subject><subject>Muscle Proteins - isolation & purification</subject><subject>product authenticity</subject><subject>protein composition</subject><subject>proteome</subject><subject>Proteome - isolation & purification</subject><subject>proteomics</subject><subject>Solubility</subject><subject>Species Specificity</subject><subject>Tetraodontidae</subject><subject>Tetraodontiformes - classification</subject><subject>Urea</subject><subject>urea soluble proteins</subject><issn>0021-8561</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0s1u1DAQB3ALgehSOPACkAvc0o7t2E6OqB-0UiUi7VYcLdtxqJckXmxHsE_Cq_BUPANe7bbQE6c5_H8e26NB6DWGEwwEn657oAwonZ6gBWYESoZx_RQtIIdlzTg-Qi9iXANAzQQ8R0eYQQ2ciAVKbbCDG92kwrZYprnbFn4q2rnvbSguXbwr2uCT9aP9_fPXcmONs7G47uyUXO-MSi5r3z86oLfF6rsvz91op5hzNRQXgzUp-M2dDza6-BI969UQ7atDPUa3lxers6vy5tPH67MPN6WiHFJZGWoaKxR0jGmCK70rIKgGVRHDDBFaa645Ex0njDSqo6TW1PD8e0P7mh6j9_u-m-C_zTYmObpo7DCoyfo5SoFrKjBu_gtxU-GGsB18c4CzHm0nN8GNeXLyfqAZvDsAFY0a-qAm4-I_rsEcGppduXcuJvvjIVfhq-SCCiZX7VIyfkXgM6llm_3bve-Vl-pLyD1vlwQwhdytAkb-3qxMlGs_hzz4_HKQuxWRDytC_wBDyalQ</recordid><startdate>20040421</startdate><enddate>20040421</enddate><creator>Chen, Tai-Yuan</creator><creator>Shiau, Chyuan-Yuan</creator><creator>Wei, Cheng-I</creator><creator>Hwang, Deng-Fwu</creator><general>American Chemical Society</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7TN</scope><scope>8FD</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20040421</creationdate><title>Preliminary Study on Puffer Fish ProteomeSpecies Identification of Puffer Fish by Two-Dimensional Electrophoresis</title><author>Chen, Tai-Yuan ; Shiau, Chyuan-Yuan ; Wei, Cheng-I ; Hwang, Deng-Fwu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a360t-4c3c9e7a0d55b214b55b2073b0a42c5c27bbb6b657d62529ad328b3c6118c3f83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>animal proteins</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Databases as Topic</topic><topic>Databases, Protein</topic><topic>Electrophoresis, Gel, Two-Dimensional</topic><topic>Fish and seafood industries</topic><topic>fish products</topic><topic>food analysis</topic><topic>Food industries</topic><topic>Fugu</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gel electrophoresis</topic><topic>Hydrogen-Ion Concentration</topic><topic>Isoelectric Point</topic><topic>Lagocephalus</topic><topic>marine fish</topic><topic>Molecular Weight</topic><topic>Muscle Proteins - chemistry</topic><topic>Muscle Proteins - isolation & purification</topic><topic>product authenticity</topic><topic>protein composition</topic><topic>proteome</topic><topic>Proteome - isolation & purification</topic><topic>proteomics</topic><topic>Solubility</topic><topic>Species Specificity</topic><topic>Tetraodontidae</topic><topic>Tetraodontiformes - classification</topic><topic>Urea</topic><topic>urea soluble proteins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Tai-Yuan</creatorcontrib><creatorcontrib>Shiau, Chyuan-Yuan</creatorcontrib><creatorcontrib>Wei, Cheng-I</creatorcontrib><creatorcontrib>Hwang, Deng-Fwu</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Oceanic Abstracts</collection><collection>Technology Research Database</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of agricultural and food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Tai-Yuan</au><au>Shiau, Chyuan-Yuan</au><au>Wei, Cheng-I</au><au>Hwang, Deng-Fwu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Preliminary Study on Puffer Fish ProteomeSpecies Identification of Puffer Fish by Two-Dimensional Electrophoresis</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><addtitle>J. Agric. Food Chem</addtitle><date>2004-04-21</date><risdate>2004</risdate><volume>52</volume><issue>8</issue><spage>2236</spage><epage>2241</epage><pages>2236-2241</pages><issn>0021-8561</issn><eissn>1520-5118</eissn><coden>JAFCAU</coden><abstract>The aims of this work were to determine the differential characterization of the urea soluble protein components of puffer fish species and to establish a preliminary proteomic database using an immobilized pH gradient two-dimensional electrophoresis (2DE) technique. The puffer fish muscle proteins resolved into 171−260 spots in the 2DE gels, with a pI range of 3−10 and molecular mass range of 7.4−205.0 kDa, following Comassie blue staining. Puffer fish muscle proteins fell in the region with pI values of 3.5−7.0, and molecular masses of 7.4−45.0 kDa were well-resolved and were good for species comparison. The more acidic proteins of lower molecular masses showed species specific characteristics. Therefore, the species of puffer fish can be differentiated from the comparison of the characteristic 2DE protein patterns. Keywords: Two-dimensional electrophoresis; puffer fish; species identification; proteome; protein</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>15080627</pmid><doi>10.1021/jf035033n</doi><tpages>6</tpages></addata></record> |
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| subjects | animal proteins Animals Biological and medical sciences Databases as Topic Databases, Protein Electrophoresis, Gel, Two-Dimensional Fish and seafood industries fish products food analysis Food industries Fugu Fundamental and applied biological sciences. Psychology gel electrophoresis Hydrogen-Ion Concentration Isoelectric Point Lagocephalus marine fish Molecular Weight Muscle Proteins - chemistry Muscle Proteins - isolation & purification product authenticity protein composition proteome Proteome - isolation & purification proteomics Solubility Species Specificity Tetraodontidae Tetraodontiformes - classification Urea urea soluble proteins |
| title | Preliminary Study on Puffer Fish ProteomeSpecies Identification of Puffer Fish by Two-Dimensional Electrophoresis |
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