Enzymatic assay for perfluoro-tagged metabolites of L-DOPA using crude lysate from E. coli transformed with pKKAADCII

Isomers of L-DOPA and dopamine with a nine-atom ¹⁹F atom tag were exposed to aromatic acid decarboxylase (AADC) in the lysate of Escherichia coli JM109 that had been transformed with the plasmid pKKAADCII. The resulting samples were analyzed with HPLC. The first study investigated the conversion of...

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Veröffentlicht in:Applied microbiology and biotechnology 2004-05, Vol.64 (4), p.556-559
Hauptverfasser: Dingman, S, Snyder-Leiby, T, Mack, D. J, Thomas, R, Guo, C
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Sprache:eng
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