Enzymatic assay for perfluoro-tagged metabolites of L-DOPA using crude lysate from E. coli transformed with pKKAADCII
Isomers of L-DOPA and dopamine with a nine-atom ¹⁹F atom tag were exposed to aromatic acid decarboxylase (AADC) in the lysate of Escherichia coli JM109 that had been transformed with the plasmid pKKAADCII. The resulting samples were analyzed with HPLC. The first study investigated the conversion of...
Gespeichert in:
Veröffentlicht in: | Applied microbiology and biotechnology 2004-05, Vol.64 (4), p.556-559 |
---|---|
Hauptverfasser: | , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Schreiben Sie den ersten Kommentar!