Phosphatidylserine Efflux and Intercellular Fusion in a BeWo Model of Human Villous Cytotrophoblast

Phosphatidylserine (PS) efflux characterizes cytotrophoblast apoptosis and differentiation. To evaluate whether PS externalization and intercellular fusion were secondary to apoptosis, BeWo cells were induced to differentiate by forskolin or undergo apoptosis by staurosporine. PS externalization was...

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Veröffentlicht in:	Placenta (Eastbourne) 2004-05, Vol.25 (5), p.396-407
Hauptverfasser:	Das, M., Xu, B., Lin, L., Chakrabarti, S., Shivaswamy, V., Rote, N.S.
Format:	Artikel
Sprache:	eng
Schlagworte:	Ageing, cell death Amino Acid Chloromethyl Ketones - pharmacology Apoptosis - drug effects Apoptosis - physiology Apoptosis Inducing Factor Biological and medical sciences Blotting, Western Caspase 3 Caspase Inhibitors Caspases - metabolism Cell Differentiation - drug effects Cell Differentiation - physiology Cell Fusion Cell Line, Tumor Cell Membrane - drug effects Cell Membrane - metabolism Cell physiology Colforsin - pharmacology Cysteine Proteinase Inhibitors - pharmacology Cytochromes c - antagonists & inhibitors Cytochromes c - metabolism Cytosol - chemistry DNA Fragmentation - drug effects Flavoproteins - metabolism Fundamental and applied biological sciences. Psychology Humans Lamin Type B - metabolism Membrane Lipids - metabolism Membrane Potentials - drug effects Membrane Proteins - metabolism Microscopy, Fluorescence Mitochondria - chemistry Mitochondria - drug effects Mitochondria - metabolism Models, Biological Molecular and cellular biology Phosphatidylserines - metabolism Staurosporine - pharmacology Trophoblasts - cytology Trophoblasts - drug effects Trophoblasts - physiology Vanadates - pharmacology
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Zusammenfassung:	Phosphatidylserine (PS) efflux characterizes cytotrophoblast apoptosis and differentiation. To evaluate whether PS externalization and intercellular fusion were secondary to apoptosis, BeWo cells were induced to differentiate by forskolin or undergo apoptosis by staurosporine. PS externalization was measured by FITC–annexin V binding, and intercellular fusion was quantified by counting nuclei in syncytial cells. During forskolin treatment, vanadate decreased PS efflux by 78.0 per cent from 68.0 [5.3] (mean [SD]) to 15.0 [8.8] Lum (×10 3) ( P
ISSN:	0143-4004 1532-3102
DOI:	10.1016/j.placenta.2003.11.004