A homolog of Escherichia coli RecA in mitochondria of the cellular slime mold Dictyostelium discoideum
The cellular slime mold Dictyostelium discoideum expresses a gene encoding a 452-amino-acid polypeptide that is 47% identical to Escherichia coli RecA. A recA-deficient E. coli, JE6651, was transformed by pYSN1, which was designed to express the truncated form of the D. discoideum gene, and used in...
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| Veröffentlicht in: | DNA repair 2004-05, Vol.3 (5), p.515-525 |
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| creator | Hasegawa, Yasuna Wakabayashi, Masayuki Nakamura, Shogo Kodaira, Ken-ichi Shinohara, Hiroaki Yasukawa, Hiro |
| description | The cellular slime mold
Dictyostelium discoideum expresses a gene encoding a 452-amino-acid polypeptide that is 47% identical to
Escherichia coli RecA. A
recA-deficient
E. coli, JE6651, was transformed by pYSN1, which was designed to express the truncated form of the
D. discoideum gene, and used in suppression assays. The viability of the transformant, JE6651(pYSN1), increased following UV irradiation or mitomycin C treatment. Phage lambda (
red
−
gam
−), which required RecA activity for DNA packaging, formed plaques on a lawn of JE6651(pYSN1). These results indicate that the gene product has a DNA recombination activity. Fluorescence of
D. discoideum protein fused with GFP was detected in mitochondria. The gene disruption mutant was hypersensitive to UV-light (254
nm), mitomycin C and H
2O
2, indicating that
D. discoideum recA is important for survival following exposure to DNA damaging agents. |
| doi_str_mv | 10.1016/j.dnarep.2004.01.014 |
| format | Article |
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Dictyostelium discoideum expresses a gene encoding a 452-amino-acid polypeptide that is 47% identical to
Escherichia coli RecA. A
recA-deficient
E. coli, JE6651, was transformed by pYSN1, which was designed to express the truncated form of the
D. discoideum gene, and used in suppression assays. The viability of the transformant, JE6651(pYSN1), increased following UV irradiation or mitomycin C treatment. Phage lambda (
red
−
gam
−), which required RecA activity for DNA packaging, formed plaques on a lawn of JE6651(pYSN1). These results indicate that the gene product has a DNA recombination activity. Fluorescence of
D. discoideum protein fused with GFP was detected in mitochondria. The gene disruption mutant was hypersensitive to UV-light (254
nm), mitomycin C and H
2O
2, indicating that
D. discoideum recA is important for survival following exposure to DNA damaging agents.</description><identifier>ISSN: 1568-7864</identifier><identifier>EISSN: 1568-7856</identifier><identifier>DOI: 10.1016/j.dnarep.2004.01.014</identifier><identifier>PMID: 15084313</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Amino Acid Sequence ; Animals ; Bacteriology ; Base Sequence ; Biological and medical sciences ; Cloning, Molecular ; Dictyostelium - cytology ; Dictyostelium - drug effects ; Dictyostelium - enzymology ; Dictyostelium - genetics ; Dictyostelium discoideum ; DNA Damage - drug effects ; DNA Damage - radiation effects ; DNA repair ; Escherichia coli ; Escherichia coli - enzymology ; Escherichia coli - genetics ; Fundamental and applied biological sciences. Psychology ; Growth, nutrition, cell differenciation ; Hydrogen Peroxide - pharmacology ; Microbiology ; Mitochondria - metabolism ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Mutagenesis. Repair ; Mutation - genetics ; Protein Transport ; Rec A Recombinases - chemistry ; Rec A Recombinases - genetics ; Rec A Recombinases - metabolism ; recA ; recombination ; Ultraviolet Rays</subject><ispartof>DNA repair, 2004-05, Vol.3 (5), p.515-525</ispartof><rights>2004 Elsevier B.V.</rights><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c485t-aca5128c2efa30f69f9e6aaa43d7acca460eaef84e22e9a98d68a5df1204fe643</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.dnarep.2004.01.014$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15656467$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15084313$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hasegawa, Yasuna</creatorcontrib><creatorcontrib>Wakabayashi, Masayuki</creatorcontrib><creatorcontrib>Nakamura, Shogo</creatorcontrib><creatorcontrib>Kodaira, Ken-ichi</creatorcontrib><creatorcontrib>Shinohara, Hiroaki</creatorcontrib><creatorcontrib>Yasukawa, Hiro</creatorcontrib><title>A homolog of Escherichia coli RecA in mitochondria of the cellular slime mold Dictyostelium discoideum</title><title>DNA repair</title><addtitle>DNA Repair (Amst)</addtitle><description>The cellular slime mold
Dictyostelium discoideum expresses a gene encoding a 452-amino-acid polypeptide that is 47% identical to
Escherichia coli RecA. A
recA-deficient
E. coli, JE6651, was transformed by pYSN1, which was designed to express the truncated form of the
D. discoideum gene, and used in suppression assays. The viability of the transformant, JE6651(pYSN1), increased following UV irradiation or mitomycin C treatment. Phage lambda (
red
−
gam
−), which required RecA activity for DNA packaging, formed plaques on a lawn of JE6651(pYSN1). These results indicate that the gene product has a DNA recombination activity. Fluorescence of
D. discoideum protein fused with GFP was detected in mitochondria. The gene disruption mutant was hypersensitive to UV-light (254
nm), mitomycin C and H
2O
2, indicating that
D. discoideum recA is important for survival following exposure to DNA damaging agents.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Bacteriology</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cloning, Molecular</subject><subject>Dictyostelium - cytology</subject><subject>Dictyostelium - drug effects</subject><subject>Dictyostelium - enzymology</subject><subject>Dictyostelium - genetics</subject><subject>Dictyostelium discoideum</subject><subject>DNA Damage - drug effects</subject><subject>DNA Damage - radiation effects</subject><subject>DNA repair</subject><subject>Escherichia coli</subject><subject>Escherichia coli - enzymology</subject><subject>Escherichia coli - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Growth, nutrition, cell differenciation</subject><subject>Hydrogen Peroxide - pharmacology</subject><subject>Microbiology</subject><subject>Mitochondria - metabolism</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Mutagenesis. Repair</subject><subject>Mutation - genetics</subject><subject>Protein Transport</subject><subject>Rec A Recombinases - chemistry</subject><subject>Rec A Recombinases - genetics</subject><subject>Rec A Recombinases - metabolism</subject><subject>recA</subject><subject>recombination</subject><subject>Ultraviolet Rays</subject><issn>1568-7864</issn><issn>1568-7856</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUGLFDEQhYMo7rr6D0Ry0duMSXc6nb4Iw7q6woIgeg5lpWJnSHfGpFvYf2-GGdTTCgUJ1FePx3uMvZRiK4XUb_dbN0Omw7YRQm2FrKMesUvZabPpTacf__lrdcGelbIXQna91k_ZheyEUa1sL5nf8TFNKaYfPHl-U3CkHHAMwDHFwL8Q7niY-RSWhGOaXa6bCi4jcaQY1wiZlxgm4lXE8fcBl_tUFophnbgLBVNwtE7P2RMPsdCL83vFvn24-Xp9u7n7_PHT9e5ug8p0ywYQOtkYbMhDK7we_EAaAFTrekAEpQUBeaOoaWiAwThtoHNeNkJ50qq9Ym9Ouoecfq5UFjtVD9UozJTWYntpmmGQw39BaWpapj0qqhOIOZWSydtDDhPkeyuFPRZh9_ZUhD0WYYWsczx7ddZfv0_k_h6dk6_A6zMABSH6DDOG8g-nO610X7l3J45qbL8CZVsw0IzkQiZcrEvhYSe_Aa6Eqfc</recordid><startdate>20040504</startdate><enddate>20040504</enddate><creator>Hasegawa, Yasuna</creator><creator>Wakabayashi, Masayuki</creator><creator>Nakamura, Shogo</creator><creator>Kodaira, Ken-ichi</creator><creator>Shinohara, Hiroaki</creator><creator>Yasukawa, Hiro</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>20040504</creationdate><title>A homolog of Escherichia coli RecA in mitochondria of the cellular slime mold Dictyostelium discoideum</title><author>Hasegawa, Yasuna ; Wakabayashi, Masayuki ; Nakamura, Shogo ; Kodaira, Ken-ichi ; Shinohara, Hiroaki ; Yasukawa, Hiro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c485t-aca5128c2efa30f69f9e6aaa43d7acca460eaef84e22e9a98d68a5df1204fe643</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Bacteriology</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Cloning, Molecular</topic><topic>Dictyostelium - cytology</topic><topic>Dictyostelium - drug effects</topic><topic>Dictyostelium - enzymology</topic><topic>Dictyostelium - genetics</topic><topic>Dictyostelium discoideum</topic><topic>DNA Damage - drug effects</topic><topic>DNA Damage - radiation effects</topic><topic>DNA repair</topic><topic>Escherichia coli</topic><topic>Escherichia coli - enzymology</topic><topic>Escherichia coli - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Growth, nutrition, cell differenciation</topic><topic>Hydrogen Peroxide - pharmacology</topic><topic>Microbiology</topic><topic>Mitochondria - metabolism</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Mutagenesis. Repair</topic><topic>Mutation - genetics</topic><topic>Protein Transport</topic><topic>Rec A Recombinases - chemistry</topic><topic>Rec A Recombinases - genetics</topic><topic>Rec A Recombinases - metabolism</topic><topic>recA</topic><topic>recombination</topic><topic>Ultraviolet Rays</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hasegawa, Yasuna</creatorcontrib><creatorcontrib>Wakabayashi, Masayuki</creatorcontrib><creatorcontrib>Nakamura, Shogo</creatorcontrib><creatorcontrib>Kodaira, Ken-ichi</creatorcontrib><creatorcontrib>Shinohara, Hiroaki</creatorcontrib><creatorcontrib>Yasukawa, Hiro</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>DNA repair</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hasegawa, Yasuna</au><au>Wakabayashi, Masayuki</au><au>Nakamura, Shogo</au><au>Kodaira, Ken-ichi</au><au>Shinohara, Hiroaki</au><au>Yasukawa, Hiro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A homolog of Escherichia coli RecA in mitochondria of the cellular slime mold Dictyostelium discoideum</atitle><jtitle>DNA repair</jtitle><addtitle>DNA Repair (Amst)</addtitle><date>2004-05-04</date><risdate>2004</risdate><volume>3</volume><issue>5</issue><spage>515</spage><epage>525</epage><pages>515-525</pages><issn>1568-7864</issn><eissn>1568-7856</eissn><abstract>The cellular slime mold
Dictyostelium discoideum expresses a gene encoding a 452-amino-acid polypeptide that is 47% identical to
Escherichia coli RecA. A
recA-deficient
E. coli, JE6651, was transformed by pYSN1, which was designed to express the truncated form of the
D. discoideum gene, and used in suppression assays. The viability of the transformant, JE6651(pYSN1), increased following UV irradiation or mitomycin C treatment. Phage lambda (
red
−
gam
−), which required RecA activity for DNA packaging, formed plaques on a lawn of JE6651(pYSN1). These results indicate that the gene product has a DNA recombination activity. Fluorescence of
D. discoideum protein fused with GFP was detected in mitochondria. The gene disruption mutant was hypersensitive to UV-light (254
nm), mitomycin C and H
2O
2, indicating that
D. discoideum recA is important for survival following exposure to DNA damaging agents.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>15084313</pmid><doi>10.1016/j.dnarep.2004.01.014</doi><tpages>11</tpages></addata></record> |
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| subjects | Amino Acid Sequence Animals Bacteriology Base Sequence Biological and medical sciences Cloning, Molecular Dictyostelium - cytology Dictyostelium - drug effects Dictyostelium - enzymology Dictyostelium - genetics Dictyostelium discoideum DNA Damage - drug effects DNA Damage - radiation effects DNA repair Escherichia coli Escherichia coli - enzymology Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Growth, nutrition, cell differenciation Hydrogen Peroxide - pharmacology Microbiology Mitochondria - metabolism Molecular and cellular biology Molecular genetics Molecular Sequence Data Mutagenesis. Repair Mutation - genetics Protein Transport Rec A Recombinases - chemistry Rec A Recombinases - genetics Rec A Recombinases - metabolism recA recombination Ultraviolet Rays |
| title | A homolog of Escherichia coli RecA in mitochondria of the cellular slime mold Dictyostelium discoideum |
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