Direct modulation of the effective sensitivity of a CCD detector: a new approach to time‐resolved fluorescence imaging
Summary In this paper a novel approach to frequency‐domain fluorescence lifetime imaging (FLIM) is described. In a CCD camera a single pixel is defined by a charge pattern on a group of electrodes. By modulation of the pattern of voltages defining the pixel structure it is possible to modulate the s...
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| Veröffentlicht in: | Journal of microscopy (Oxford) 2002-06, Vol.206 (3), p.225-232 |
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| container_title | Journal of microscopy (Oxford) |
| container_volume | 206 |
| creator | Mitchell, A. C. Wall, J. E. Murray, J. G. Morgan, C. G. |
| description | Summary
In this paper a novel approach to frequency‐domain fluorescence lifetime imaging (FLIM) is described. In a CCD camera a single pixel is defined by a charge pattern on a group of electrodes. By modulation of the pattern of voltages defining the pixel structure it is possible to modulate the sensitivity of the CCD at radio frequency. The modulation enhances the noise performance of the CCD, in contrast to the deterioration in performance seen when an intensifier stage is similarly modulated. The new technology has potential applications to a wide range of assays as well as in conventional FLIM applications. Unlike intensifier‐based systems, the directly modulated CCD is physically small, inexpensive, robust and offers superior resolution and noise performance. |
| doi_str_mv | 10.1046/j.1365-2818.2002.01029.x |
| format | Article |
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In this paper a novel approach to frequency‐domain fluorescence lifetime imaging (FLIM) is described. In a CCD camera a single pixel is defined by a charge pattern on a group of electrodes. By modulation of the pattern of voltages defining the pixel structure it is possible to modulate the sensitivity of the CCD at radio frequency. The modulation enhances the noise performance of the CCD, in contrast to the deterioration in performance seen when an intensifier stage is similarly modulated. The new technology has potential applications to a wide range of assays as well as in conventional FLIM applications. Unlike intensifier‐based systems, the directly modulated CCD is physically small, inexpensive, robust and offers superior resolution and noise performance.</description><identifier>ISSN: 0022-2720</identifier><identifier>EISSN: 1365-2818</identifier><identifier>DOI: 10.1046/j.1365-2818.2002.01029.x</identifier><identifier>PMID: 12067367</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Ltd</publisher><subject>CCD ; FLIM ; fluorescence lifetime imaging ; modulation ; phase‐shift ; time‐resolved detection</subject><ispartof>Journal of microscopy (Oxford), 2002-06, Vol.206 (3), p.225-232</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3659-c51c57d58a370a38fe3567594779b64f21ce9ece2bbb20a6648aac2b681333d63</citedby><cites>FETCH-LOGICAL-c3659-c51c57d58a370a38fe3567594779b64f21ce9ece2bbb20a6648aac2b681333d63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1365-2818.2002.01029.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.1365-2818.2002.01029.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,1427,27901,27902,45550,45551,46384,46808</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12067367$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mitchell, A. C.</creatorcontrib><creatorcontrib>Wall, J. E.</creatorcontrib><creatorcontrib>Murray, J. G.</creatorcontrib><creatorcontrib>Morgan, C. G.</creatorcontrib><title>Direct modulation of the effective sensitivity of a CCD detector: a new approach to time‐resolved fluorescence imaging</title><title>Journal of microscopy (Oxford)</title><addtitle>J Microsc</addtitle><description>Summary
In this paper a novel approach to frequency‐domain fluorescence lifetime imaging (FLIM) is described. In a CCD camera a single pixel is defined by a charge pattern on a group of electrodes. By modulation of the pattern of voltages defining the pixel structure it is possible to modulate the sensitivity of the CCD at radio frequency. The modulation enhances the noise performance of the CCD, in contrast to the deterioration in performance seen when an intensifier stage is similarly modulated. The new technology has potential applications to a wide range of assays as well as in conventional FLIM applications. Unlike intensifier‐based systems, the directly modulated CCD is physically small, inexpensive, robust and offers superior resolution and noise performance.</description><subject>CCD</subject><subject>FLIM</subject><subject>fluorescence lifetime imaging</subject><subject>modulation</subject><subject>phase‐shift</subject><subject>time‐resolved detection</subject><issn>0022-2720</issn><issn>1365-2818</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><recordid>eNqNkMtO5DAQRS00I2gev4C8YpfgR2I7SCxGzWNAIDawthynAm4lcU-cQPeOT-Ab-ZJxpluwnZWrdG9VXR-EMCUpJZk4XaSUizxhiqqUEcJSQgkr0tUOmn0JP9AsKixhkpE9tB_CghCickV20R5lREgu5AytLlwPdsCtr8bGDM532Nd4eAEMdR0F9wo4QBdcrNywnkSD5_MLXMEQZd-fxb6DN2yWy94b-4IHjwfXwuf7Rw_BN69Q4boZfWwsdBawa82z654P0c_aNAGOtu8Berq6fJz_Tu4erm_mv-4SGz9SJDanNpdVrgyXxHBVA8-FzItMyqIUWc2ohQIssLIsGTFCZMoYy0qhKOe8EvwAnWz2xnh_RgiDbl1M0jSmAz8GLaliKuOTUW2Mtvch9FDrZR-z9mtNiZ6o64We4OoJrp6o63_U9SqOHm9vjGUL1ffgFnM0nG8Mb66B9X8v1rf3N1PF_wLhtZKr</recordid><startdate>200206</startdate><enddate>200206</enddate><creator>Mitchell, A. C.</creator><creator>Wall, J. E.</creator><creator>Murray, J. G.</creator><creator>Morgan, C. G.</creator><general>Blackwell Science Ltd</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200206</creationdate><title>Direct modulation of the effective sensitivity of a CCD detector: a new approach to time‐resolved fluorescence imaging</title><author>Mitchell, A. C. ; Wall, J. E. ; Murray, J. G. ; Morgan, C. G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3659-c51c57d58a370a38fe3567594779b64f21ce9ece2bbb20a6648aac2b681333d63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>CCD</topic><topic>FLIM</topic><topic>fluorescence lifetime imaging</topic><topic>modulation</topic><topic>phase‐shift</topic><topic>time‐resolved detection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mitchell, A. C.</creatorcontrib><creatorcontrib>Wall, J. E.</creatorcontrib><creatorcontrib>Murray, J. G.</creatorcontrib><creatorcontrib>Morgan, C. G.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of microscopy (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mitchell, A. C.</au><au>Wall, J. E.</au><au>Murray, J. G.</au><au>Morgan, C. G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Direct modulation of the effective sensitivity of a CCD detector: a new approach to time‐resolved fluorescence imaging</atitle><jtitle>Journal of microscopy (Oxford)</jtitle><addtitle>J Microsc</addtitle><date>2002-06</date><risdate>2002</risdate><volume>206</volume><issue>3</issue><spage>225</spage><epage>232</epage><pages>225-232</pages><issn>0022-2720</issn><eissn>1365-2818</eissn><abstract>Summary
In this paper a novel approach to frequency‐domain fluorescence lifetime imaging (FLIM) is described. In a CCD camera a single pixel is defined by a charge pattern on a group of electrodes. By modulation of the pattern of voltages defining the pixel structure it is possible to modulate the sensitivity of the CCD at radio frequency. The modulation enhances the noise performance of the CCD, in contrast to the deterioration in performance seen when an intensifier stage is similarly modulated. The new technology has potential applications to a wide range of assays as well as in conventional FLIM applications. Unlike intensifier‐based systems, the directly modulated CCD is physically small, inexpensive, robust and offers superior resolution and noise performance.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>12067367</pmid><doi>10.1046/j.1365-2818.2002.01029.x</doi><tpages>8</tpages></addata></record> |
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| language | eng |
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| source | Wiley Free Content; Wiley Online Library Journals Frontfile Complete |
| subjects | CCD FLIM fluorescence lifetime imaging modulation phase‐shift time‐resolved detection |
| title | Direct modulation of the effective sensitivity of a CCD detector: a new approach to time‐resolved fluorescence imaging |
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