High-throughput protein arrays: prospects for molecular diagnostics
High-throughput protein arrays allow the miniaturized and parallel analysis of large numbers of diagnostic markers in complex samples. Using automated colony picking and gridding, cDNA or antibody libraries can be expressed and screened as clone arrays. Protein microarrays are constructed from recom...
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| Veröffentlicht in: | Trends in molecular medicine 2002-06, Vol.8 (6), p.250-253 |
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| container_title | Trends in molecular medicine |
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| creator | Walter, Gerald Büssow, Konrad Lueking, Angelika Glökler, Jörn |
| description | High-throughput protein arrays allow the miniaturized and parallel analysis of large numbers of diagnostic markers in complex samples. Using automated colony picking and gridding, cDNA or antibody libraries can be expressed and screened as clone arrays. Protein microarrays are constructed from recombinantly expressed, purified, and yet functional proteins, entailing a range of optimized expression systems. Antibody microarrays are becoming a robust format for expression profiling of whole genomes. Alternative systems, such as aptamer, PROfusion™, nano- and microfluidic arrays are all at proof-of-concept stage. Differential protein profiles have been used as molecular diagnostics for cancer and autoimmune diseases and might ultimately be applied to screening of high-risk and general populations.
High-throughput protein arrays are still largely experimental but have taken the first steps towards becoming diagnostic tools, which will eventually arrive at the doctor's practice and as over-the-counter devices. |
| doi_str_mv | 10.1016/S1471-4914(02)02352-3 |
| format | Article |
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High-throughput protein arrays are still largely experimental but have taken the first steps towards becoming diagnostic tools, which will eventually arrive at the doctor's practice and as over-the-counter devices.</description><subject>Animals</subject><subject>Antibodies</subject><subject>antibody array</subject><subject>aptamer</subject><subject>Gene Expression Profiling</subject><subject>high-throughput protein array</subject><subject>Humans</subject><subject>microarray</subject><subject>microfluidic array</subject><subject>Molecular Diagnostic Techniques - methods</subject><subject>nanofluidic array</subject><subject>Nanotechnology - methods</subject><subject>Oligonucleotide Array Sequence Analysis - methods</subject><subject>Peptide Library</subject><subject>PROfusion</subject><subject>Proteomics - methods</subject><issn>1471-4914</issn><issn>1471-499X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMtKAzEUhoMotlYfQZmV6GI0t0kybooUtULBhQruQprJtJG5mWSEvr1pO-jSzbnxn9sHwDmCNwgidvuKKEcpzRG9gvgaYpLhlByA8VDOPw5_Y0RH4MT7TwhRxrk4BiOEIeMM0jGYze1qnYa1a_vVuutD0rk2GNskyjm18Xfb3HdGB5-UrUvqtjK6r5RLCqtWTeuD1f4UHJWq8uZs8BPw_vjwNpuni5en59n9ItUZFCFlNKd4iVTOiNC8iIZirgrBKOFlkaNSM5ZzwrhgmSKZMJSVvFxmGmOCERNkAi73c-NNX73xQdbWa1NVqjFt7yVHAjPKeBRme6GOx3tnStk5Wyu3kQjKLT25oye3aCTEckdPkth3MSzol7Up_roGXFEw3QtMfPPbGie9tqbRprAuMpJFa_9Z8QMgmn5e</recordid><startdate>20020601</startdate><enddate>20020601</enddate><creator>Walter, Gerald</creator><creator>Büssow, Konrad</creator><creator>Lueking, Angelika</creator><creator>Glökler, Jörn</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20020601</creationdate><title>High-throughput protein arrays: prospects for molecular diagnostics</title><author>Walter, Gerald ; Büssow, Konrad ; Lueking, Angelika ; Glökler, Jörn</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c508t-64942b1a9638c7d38c427ad86437fd91fc6697367865a358e46f7fb5c22321683</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Animals</topic><topic>Antibodies</topic><topic>antibody array</topic><topic>aptamer</topic><topic>Gene Expression Profiling</topic><topic>high-throughput protein array</topic><topic>Humans</topic><topic>microarray</topic><topic>microfluidic array</topic><topic>Molecular Diagnostic Techniques - methods</topic><topic>nanofluidic array</topic><topic>Nanotechnology - methods</topic><topic>Oligonucleotide Array Sequence Analysis - methods</topic><topic>Peptide Library</topic><topic>PROfusion</topic><topic>Proteomics - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Walter, Gerald</creatorcontrib><creatorcontrib>Büssow, Konrad</creatorcontrib><creatorcontrib>Lueking, Angelika</creatorcontrib><creatorcontrib>Glökler, Jörn</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Trends in molecular medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Walter, Gerald</au><au>Büssow, Konrad</au><au>Lueking, Angelika</au><au>Glökler, Jörn</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>High-throughput protein arrays: prospects for molecular diagnostics</atitle><jtitle>Trends in molecular medicine</jtitle><addtitle>Trends Mol Med</addtitle><date>2002-06-01</date><risdate>2002</risdate><volume>8</volume><issue>6</issue><spage>250</spage><epage>253</epage><pages>250-253</pages><issn>1471-4914</issn><eissn>1471-499X</eissn><abstract>High-throughput protein arrays allow the miniaturized and parallel analysis of large numbers of diagnostic markers in complex samples. Using automated colony picking and gridding, cDNA or antibody libraries can be expressed and screened as clone arrays. Protein microarrays are constructed from recombinantly expressed, purified, and yet functional proteins, entailing a range of optimized expression systems. Antibody microarrays are becoming a robust format for expression profiling of whole genomes. Alternative systems, such as aptamer, PROfusion™, nano- and microfluidic arrays are all at proof-of-concept stage. Differential protein profiles have been used as molecular diagnostics for cancer and autoimmune diseases and might ultimately be applied to screening of high-risk and general populations.
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| ispartof | Trends in molecular medicine, 2002-06, Vol.8 (6), p.250-253 |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Animals Antibodies antibody array aptamer Gene Expression Profiling high-throughput protein array Humans microarray microfluidic array Molecular Diagnostic Techniques - methods nanofluidic array Nanotechnology - methods Oligonucleotide Array Sequence Analysis - methods Peptide Library PROfusion Proteomics - methods |
| title | High-throughput protein arrays: prospects for molecular diagnostics |
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