Application of 3′-mismatched reverse primer PCR compared with real-time PCR and PCR-RFLP for the rapid detection of 23S rDNA mutations associated with clarithromycin resistance in Helicobacter pylori

Helicobacter pylori clarithromycin (Cla) resistance dramatically reduces efficacy of eradication therapy. In this study, 3′-mismatched reverse primer PCR (3M-PCR), real-time PCR (LightCycler), and PCR-RFLP assays were investigated to determine their sensitivity for detecting clarithromycin resistanc...

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Veröffentlicht in:	International journal of antimicrobial agents 2004-04, Vol.23 (4), p.349-355
Hauptverfasser:	Elviss, Nicola C, Lawson, Andrew J, Owen, Robert J
Format:	Artikel
Sprache:	eng
Schlagworte:	23S rDNA mutations Anti-Bacterial Agents - pharmacology Antibacterial agents Antibiotics. Antiinfectious agents. Antiparasitic agents Biological and medical sciences Clarithromycin - pharmacology Clarithromycin resistance DNA Primers DNA, Ribosomal - genetics Drug Resistance, Bacterial - genetics Helicobacter pylori Helicobacter pylori - drug effects Helicobacter pylori - genetics Humans Medical sciences Microbial Sensitivity Tests Mutation Pharmacology. Drug treatments Polymerase Chain Reaction - methods Polymorphism, Restriction Fragment Length RNA, Ribosomal, 23S - genetics
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