The tumor microenvironment: CXCR4 is associated with distinct protein expression patterns in neuroblastoma cells
In previous studies, we demonstrated that human neuroblastoma cells are equipped with the machinery to direct their homing to bone marrow. These tumor cells express the CXCR4 receptor for the bone marrow stroma-derived chemokine CXCL12 (SDF-1) and secrete the CXCL12 ligand. The present study was und...
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| Veröffentlicht in: | Immunology letters 2004-03, Vol.92 (1), p.163-169 |
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| creator | Nevo, Ido Sagi-Assif, Orit Meshel, Tsipi Geminder, Hila Goldberg-Bittman, Lilach Ben-Menachem, Shlomit Shalmon, Bruria Goldberg, Iris Ben-Baruch, Adit Witz, Isaac P. |
| description | In previous studies, we demonstrated that human neuroblastoma cells are equipped with the machinery to direct their homing to bone marrow. These tumor cells express the CXCR4 receptor for the bone marrow stroma-derived chemokine CXCL12 (SDF-1) and secrete the CXCL12 ligand. The present study was undertaken to explore possible differences in gene-expression patterns between neuroblastoma variants that over-express CXCR4 (designated STH cells) and those which express very little of this receptor (STL cells). The results of the study clearly indicate that these variants show a differential gene-expression profile. They differ in expression of some integrins such as VLA2, VLA3 and VLA6, of neuroendocrine-markers such as CD56 and synaptophysin, in the expression of c-kit and in the secretion of certain cytokines and growth factors such as TNFα, SDF-1, VEGF, IL-8, GM-CSF and IP-10. We hypothesize that these differences are due to an autocrine SDF-1α-CXCR4 axis. |
| doi_str_mv | 10.1016/j.imlet.2003.10.019 |
| format | Article |
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These tumor cells express the CXCR4 receptor for the bone marrow stroma-derived chemokine CXCL12 (SDF-1) and secrete the CXCL12 ligand. The present study was undertaken to explore possible differences in gene-expression patterns between neuroblastoma variants that over-express CXCR4 (designated STH cells) and those which express very little of this receptor (STL cells). The results of the study clearly indicate that these variants show a differential gene-expression profile. They differ in expression of some integrins such as VLA2, VLA3 and VLA6, of neuroendocrine-markers such as CD56 and synaptophysin, in the expression of c-kit and in the secretion of certain cytokines and growth factors such as TNFα, SDF-1, VEGF, IL-8, GM-CSF and IP-10. We hypothesize that these differences are due to an autocrine SDF-1α-CXCR4 axis.</description><identifier>ISSN: 0165-2478</identifier><identifier>EISSN: 1879-0542</identifier><identifier>DOI: 10.1016/j.imlet.2003.10.019</identifier><identifier>PMID: 15081541</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>CD56 Antigen - immunology ; Chemokine CXCL12 ; Chemokines, CXC - immunology ; Chemokines, CXC - metabolism ; CXCL12-SDF-1α ; CXCR4 ; Cytokines - immunology ; Cytokines - metabolism ; Gene expression ; Gene Expression - immunology ; Gene Expression - physiology ; Gene Expression Profiling ; Humans ; Integrins - immunology ; Integrins - metabolism ; Neuroblastoma ; Neuroblastoma - immunology ; Proto-Oncogene Proteins c-kit - immunology ; Proto-Oncogene Proteins c-kit - metabolism ; Receptors, CXCR4 - immunology ; Synaptophysin - immunology ; Synaptophysin - metabolism</subject><ispartof>Immunology letters, 2004-03, Vol.92 (1), p.163-169</ispartof><rights>2003 Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c388t-8a7cd489c8ed10eda85882ee72f8ad4e9578e0f9e5089d5b0a74f4483b52be293</citedby><cites>FETCH-LOGICAL-c388t-8a7cd489c8ed10eda85882ee72f8ad4e9578e0f9e5089d5b0a74f4483b52be293</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0165247803003146$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15081541$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nevo, Ido</creatorcontrib><creatorcontrib>Sagi-Assif, Orit</creatorcontrib><creatorcontrib>Meshel, Tsipi</creatorcontrib><creatorcontrib>Geminder, Hila</creatorcontrib><creatorcontrib>Goldberg-Bittman, Lilach</creatorcontrib><creatorcontrib>Ben-Menachem, Shlomit</creatorcontrib><creatorcontrib>Shalmon, Bruria</creatorcontrib><creatorcontrib>Goldberg, Iris</creatorcontrib><creatorcontrib>Ben-Baruch, Adit</creatorcontrib><creatorcontrib>Witz, Isaac P.</creatorcontrib><title>The tumor microenvironment: CXCR4 is associated with distinct protein expression patterns in neuroblastoma cells</title><title>Immunology letters</title><addtitle>Immunol Lett</addtitle><description>In previous studies, we demonstrated that human neuroblastoma cells are equipped with the machinery to direct their homing to bone marrow. These tumor cells express the CXCR4 receptor for the bone marrow stroma-derived chemokine CXCL12 (SDF-1) and secrete the CXCL12 ligand. The present study was undertaken to explore possible differences in gene-expression patterns between neuroblastoma variants that over-express CXCR4 (designated STH cells) and those which express very little of this receptor (STL cells). The results of the study clearly indicate that these variants show a differential gene-expression profile. They differ in expression of some integrins such as VLA2, VLA3 and VLA6, of neuroendocrine-markers such as CD56 and synaptophysin, in the expression of c-kit and in the secretion of certain cytokines and growth factors such as TNFα, SDF-1, VEGF, IL-8, GM-CSF and IP-10. We hypothesize that these differences are due to an autocrine SDF-1α-CXCR4 axis.</description><subject>CD56 Antigen - immunology</subject><subject>Chemokine CXCL12</subject><subject>Chemokines, CXC - immunology</subject><subject>Chemokines, CXC - metabolism</subject><subject>CXCL12-SDF-1α</subject><subject>CXCR4</subject><subject>Cytokines - immunology</subject><subject>Cytokines - metabolism</subject><subject>Gene expression</subject><subject>Gene Expression - immunology</subject><subject>Gene Expression - physiology</subject><subject>Gene Expression Profiling</subject><subject>Humans</subject><subject>Integrins - immunology</subject><subject>Integrins - metabolism</subject><subject>Neuroblastoma</subject><subject>Neuroblastoma - immunology</subject><subject>Proto-Oncogene Proteins c-kit - immunology</subject><subject>Proto-Oncogene Proteins c-kit - metabolism</subject><subject>Receptors, CXCR4 - immunology</subject><subject>Synaptophysin - immunology</subject><subject>Synaptophysin - metabolism</subject><issn>0165-2478</issn><issn>1879-0542</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkVFrFDEQx4Mo9lr9BILkybc9k93ETQQf5NAqFArSgm8hm8zSHLvJmsm29ds35x34pk-BP7-ZzMyPkDecbTnjH97vt2GeoGxbxrqabBnXz8iGq143TIr2OdlUSjat6NUZOUfcM8ZlJ7qX5IxLprgUfEOWmzugZZ1TpnNwOUG8DznFGWL5SHc_dz8EDUgtYnLBFvD0IZQ76gOWEF2hS04FQqTwuGRADCnSxZYCOSKtcYQ1p2GyWNJsqYNpwlfkxWgnhNen94Lcfv1ys_vWXF1fft99vmpcp1RplO2dF0o7BZ4z8FZJpVqAvh2V9QK07BWwUUPdRHs5MNuLUQjVDbIdoNXdBXl37FtH_LUCFjMHPExgI6QVTc9VyzXn_wV5r3UntahgdwTrmRAzjGbJYbb5t-HMHIyYvfljxByMHMJqpFa9PbVfhxn835qTggp8OgJQr3EfIBt0AaIDHzK4YnwK__zgCf0ooAo</recordid><startdate>20040329</startdate><enddate>20040329</enddate><creator>Nevo, Ido</creator><creator>Sagi-Assif, Orit</creator><creator>Meshel, Tsipi</creator><creator>Geminder, Hila</creator><creator>Goldberg-Bittman, Lilach</creator><creator>Ben-Menachem, Shlomit</creator><creator>Shalmon, Bruria</creator><creator>Goldberg, Iris</creator><creator>Ben-Baruch, Adit</creator><creator>Witz, Isaac P.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20040329</creationdate><title>The tumor microenvironment: CXCR4 is associated with distinct protein expression patterns in neuroblastoma cells</title><author>Nevo, Ido ; Sagi-Assif, Orit ; Meshel, Tsipi ; Geminder, Hila ; Goldberg-Bittman, Lilach ; Ben-Menachem, Shlomit ; Shalmon, Bruria ; Goldberg, Iris ; Ben-Baruch, Adit ; Witz, Isaac P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c388t-8a7cd489c8ed10eda85882ee72f8ad4e9578e0f9e5089d5b0a74f4483b52be293</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>CD56 Antigen - immunology</topic><topic>Chemokine CXCL12</topic><topic>Chemokines, CXC - immunology</topic><topic>Chemokines, CXC - metabolism</topic><topic>CXCL12-SDF-1α</topic><topic>CXCR4</topic><topic>Cytokines - immunology</topic><topic>Cytokines - metabolism</topic><topic>Gene expression</topic><topic>Gene Expression - immunology</topic><topic>Gene Expression - physiology</topic><topic>Gene Expression Profiling</topic><topic>Humans</topic><topic>Integrins - immunology</topic><topic>Integrins - metabolism</topic><topic>Neuroblastoma</topic><topic>Neuroblastoma - immunology</topic><topic>Proto-Oncogene Proteins c-kit - immunology</topic><topic>Proto-Oncogene Proteins c-kit - metabolism</topic><topic>Receptors, CXCR4 - immunology</topic><topic>Synaptophysin - immunology</topic><topic>Synaptophysin - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nevo, Ido</creatorcontrib><creatorcontrib>Sagi-Assif, Orit</creatorcontrib><creatorcontrib>Meshel, Tsipi</creatorcontrib><creatorcontrib>Geminder, Hila</creatorcontrib><creatorcontrib>Goldberg-Bittman, Lilach</creatorcontrib><creatorcontrib>Ben-Menachem, Shlomit</creatorcontrib><creatorcontrib>Shalmon, Bruria</creatorcontrib><creatorcontrib>Goldberg, Iris</creatorcontrib><creatorcontrib>Ben-Baruch, Adit</creatorcontrib><creatorcontrib>Witz, Isaac P.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Immunology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nevo, Ido</au><au>Sagi-Assif, Orit</au><au>Meshel, Tsipi</au><au>Geminder, Hila</au><au>Goldberg-Bittman, Lilach</au><au>Ben-Menachem, Shlomit</au><au>Shalmon, Bruria</au><au>Goldberg, Iris</au><au>Ben-Baruch, Adit</au><au>Witz, Isaac P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The tumor microenvironment: CXCR4 is associated with distinct protein expression patterns in neuroblastoma cells</atitle><jtitle>Immunology letters</jtitle><addtitle>Immunol Lett</addtitle><date>2004-03-29</date><risdate>2004</risdate><volume>92</volume><issue>1</issue><spage>163</spage><epage>169</epage><pages>163-169</pages><issn>0165-2478</issn><eissn>1879-0542</eissn><abstract>In previous studies, we demonstrated that human neuroblastoma cells are equipped with the machinery to direct their homing to bone marrow. These tumor cells express the CXCR4 receptor for the bone marrow stroma-derived chemokine CXCL12 (SDF-1) and secrete the CXCL12 ligand. The present study was undertaken to explore possible differences in gene-expression patterns between neuroblastoma variants that over-express CXCR4 (designated STH cells) and those which express very little of this receptor (STL cells). The results of the study clearly indicate that these variants show a differential gene-expression profile. They differ in expression of some integrins such as VLA2, VLA3 and VLA6, of neuroendocrine-markers such as CD56 and synaptophysin, in the expression of c-kit and in the secretion of certain cytokines and growth factors such as TNFα, SDF-1, VEGF, IL-8, GM-CSF and IP-10. We hypothesize that these differences are due to an autocrine SDF-1α-CXCR4 axis.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>15081541</pmid><doi>10.1016/j.imlet.2003.10.019</doi><tpages>7</tpages></addata></record> |
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| ispartof | Immunology letters, 2004-03, Vol.92 (1), p.163-169 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | CD56 Antigen - immunology Chemokine CXCL12 Chemokines, CXC - immunology Chemokines, CXC - metabolism CXCL12-SDF-1α CXCR4 Cytokines - immunology Cytokines - metabolism Gene expression Gene Expression - immunology Gene Expression - physiology Gene Expression Profiling Humans Integrins - immunology Integrins - metabolism Neuroblastoma Neuroblastoma - immunology Proto-Oncogene Proteins c-kit - immunology Proto-Oncogene Proteins c-kit - metabolism Receptors, CXCR4 - immunology Synaptophysin - immunology Synaptophysin - metabolism |
| title | The tumor microenvironment: CXCR4 is associated with distinct protein expression patterns in neuroblastoma cells |
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