Picrotoxin and bicuculline have different effects on lumbar spinal networks and motoneurons in the neonatal rat
Bicuculline is the most commonly used GABA A receptor antagonist to investigate the contribution of these receptors in motor control. However, this compound has been shown recently to potentiate the burst firing of neurons in various brain regions by blocking a calcium-activated potassium current un...
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| creator | Pflieger, Jean-François Clarac, François Vinay, Laurent |
| description | Bicuculline is the most commonly used GABA
A receptor antagonist to investigate the contribution of these receptors in motor control. However, this compound has been shown recently to potentiate the burst firing of neurons in various brain regions by blocking a calcium-activated potassium current underlying the spike after-hyperpolarization (AHP). This effect may distort our understanding of the role of GABA
A receptors at the network level. In vitro brainstem-spinal cord preparations isolated from neonatal rats were used to compare the effects of bicuculline methiodide (bicuculline-M) and picrotoxin (PTX), another GABA
A receptor antagonist, on the AHP of lumbar motoneurons as well as on spontaneous and locomotor-like motor activities. Intracellular recordings of lumbar motoneurons showed that bicuculline-M (20 μM) reduced the AHP to 57% of control whereas PTX (20–60 μM) had no significant effect. Bath-application of increasing concentrations of PTX caused an increase in spontaneous ventral root activity, which further increased significantly when bicuculline-M was added. The effects of both antagonists were tested on fictive locomotion. The left–right alternation was disrupted in the presence of bicuculline-M. A slow synchronous bursting activity of large amplitude also appeared in the presence of PTX. This slow rhythm was superimposed on a faster rhythm which still exhibited some degree of left–right alternation. These data demonstrate that bicuculline-M may not reveal accurately the contribution of GABA
A receptors in motor control and the intrinsic properties of disinhibited networks. |
| doi_str_mv | 10.1016/S0006-8993(02)02469-1 |
| format | Article |
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A receptors at the network level. In vitro brainstem-spinal cord preparations isolated from neonatal rats were used to compare the effects of bicuculline methiodide (bicuculline-M) and picrotoxin (PTX), another GABA
A receptor antagonist, on the AHP of lumbar motoneurons as well as on spontaneous and locomotor-like motor activities. Intracellular recordings of lumbar motoneurons showed that bicuculline-M (20 μM) reduced the AHP to 57% of control whereas PTX (20–60 μM) had no significant effect. Bath-application of increasing concentrations of PTX caused an increase in spontaneous ventral root activity, which further increased significantly when bicuculline-M was added. The effects of both antagonists were tested on fictive locomotion. The left–right alternation was disrupted in the presence of bicuculline-M. A slow synchronous bursting activity of large amplitude also appeared in the presence of PTX. This slow rhythm was superimposed on a faster rhythm which still exhibited some degree of left–right alternation. These data demonstrate that bicuculline-M may not reveal accurately the contribution of GABA
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A receptor antagonist to investigate the contribution of these receptors in motor control. However, this compound has been shown recently to potentiate the burst firing of neurons in various brain regions by blocking a calcium-activated potassium current underlying the spike after-hyperpolarization (AHP). This effect may distort our understanding of the role of GABA
A receptors at the network level. In vitro brainstem-spinal cord preparations isolated from neonatal rats were used to compare the effects of bicuculline methiodide (bicuculline-M) and picrotoxin (PTX), another GABA
A receptor antagonist, on the AHP of lumbar motoneurons as well as on spontaneous and locomotor-like motor activities. Intracellular recordings of lumbar motoneurons showed that bicuculline-M (20 μM) reduced the AHP to 57% of control whereas PTX (20–60 μM) had no significant effect. Bath-application of increasing concentrations of PTX caused an increase in spontaneous ventral root activity, which further increased significantly when bicuculline-M was added. The effects of both antagonists were tested on fictive locomotion. The left–right alternation was disrupted in the presence of bicuculline-M. A slow synchronous bursting activity of large amplitude also appeared in the presence of PTX. This slow rhythm was superimposed on a faster rhythm which still exhibited some degree of left–right alternation. These data demonstrate that bicuculline-M may not reveal accurately the contribution of GABA
A receptors in motor control and the intrinsic properties of disinhibited networks.</description><subject>2-Amino-5-phosphonovalerate - pharmacology</subject><subject>6-Cyano-7-nitroquinoxaline-2,3-dione - pharmacology</subject><subject>Action Potentials - drug effects</subject><subject>Action Potentials - physiology</subject><subject>Animals</subject><subject>Animals, Newborn</subject><subject>Bicuculline</subject><subject>Bicuculline - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Central pattern generator</subject><subject>Disinhibition</subject><subject>Excitatory Amino Acid Antagonists</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GABA A receptor</subject><subject>GABA Antagonists - pharmacology</subject><subject>GABA-A Receptor Antagonists</subject><subject>gamma-Aminobutyric Acid - metabolism</subject><subject>Interneurons - cytology</subject><subject>Interneurons - drug effects</subject><subject>Interneurons - metabolism</subject><subject>Locomotion</subject><subject>Motor control and motor pathways. Reflexes. Control centers of vegetative functions. Vestibular system and equilibration</subject><subject>Motor Neurons - cytology</subject><subject>Motor Neurons - drug effects</subject><subject>Motor Neurons - metabolism</subject><subject>Nerve Net - cytology</subject><subject>Nerve Net - drug effects</subject><subject>Nerve Net - metabolism</subject><subject>Neural Inhibition - drug effects</subject><subject>Neural Inhibition - physiology</subject><subject>Neural Pathways - cytology</subject><subject>Neural Pathways - drug effects</subject><subject>Neural Pathways - metabolism</subject><subject>Picrotoxin - pharmacology</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Receptors, GABA-A - metabolism</subject><subject>Spinal Cord - cytology</subject><subject>Spinal Cord - drug effects</subject><subject>Spinal Cord - metabolism</subject><subject>Synaptic Transmission - drug effects</subject><subject>Synaptic Transmission - physiology</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0006-8993</issn><issn>1872-6240</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtv1TAQha0KRC-Fn9DKGypYBDy24ySrqqp4SZVAAtaW40xUt4l9azsF_j3OvVd02ZUf882Z0TmEnAJ7DwzUhx-MMVW1XSfeMv6Ocam6Co7IBtqGV4pL9oxs_iPH5GVKt-UpRMdekGPgrCCN2pDw3dkYcvjjPDV-oL2zi12myXmkN-YB6eDGESP6TLFcbE40eDotc28iTVvnzUQ95t8h3qWdwFzEPC4x-ESLZr7BUg_e5AJGk1-R56OZEr4-nCfk16ePP6--VNffPn-9uryurGzbXGHPbIdiHKGTEu1Q91LUDVd2sHItGIvQ10Y0UH5NI1a8x5qNqgbeciFOyPledxvD_YIp69kli9NkyjZL0g20oIC1T4LQik4yJQtY78HiV0oRR72Nbjbxrwam10j0LhK9-q0Z17tINJS-s8OApZ9xeOw6ZFCANwfAJGumMRpvXXrkJKimk6vQxZ7D4tuDw6iTdegtDi6WYPQQ3BOr_AMrxan8</recordid><startdate>20020510</startdate><enddate>20020510</enddate><creator>Pflieger, Jean-François</creator><creator>Clarac, François</creator><creator>Vinay, Laurent</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>20020510</creationdate><title>Picrotoxin and bicuculline have different effects on lumbar spinal networks and motoneurons in the neonatal rat</title><author>Pflieger, Jean-François ; Clarac, François ; Vinay, Laurent</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c488t-eb0c9e3ff1944ecd5b435726cdc4c9e3ace1b5a371572a73b0c9be50f65128233</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>2-Amino-5-phosphonovalerate - pharmacology</topic><topic>6-Cyano-7-nitroquinoxaline-2,3-dione - pharmacology</topic><topic>Action Potentials - drug effects</topic><topic>Action Potentials - physiology</topic><topic>Animals</topic><topic>Animals, Newborn</topic><topic>Bicuculline</topic><topic>Bicuculline - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Central pattern generator</topic><topic>Disinhibition</topic><topic>Excitatory Amino Acid Antagonists</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GABA A receptor</topic><topic>GABA Antagonists - pharmacology</topic><topic>GABA-A Receptor Antagonists</topic><topic>gamma-Aminobutyric Acid - metabolism</topic><topic>Interneurons - cytology</topic><topic>Interneurons - drug effects</topic><topic>Interneurons - metabolism</topic><topic>Locomotion</topic><topic>Motor control and motor pathways. Reflexes. Control centers of vegetative functions. Vestibular system and equilibration</topic><topic>Motor Neurons - cytology</topic><topic>Motor Neurons - drug effects</topic><topic>Motor Neurons - metabolism</topic><topic>Nerve Net - cytology</topic><topic>Nerve Net - drug effects</topic><topic>Nerve Net - metabolism</topic><topic>Neural Inhibition - drug effects</topic><topic>Neural Inhibition - physiology</topic><topic>Neural Pathways - cytology</topic><topic>Neural Pathways - drug effects</topic><topic>Neural Pathways - metabolism</topic><topic>Picrotoxin - pharmacology</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Receptors, GABA-A - metabolism</topic><topic>Spinal Cord - cytology</topic><topic>Spinal Cord - drug effects</topic><topic>Spinal Cord - metabolism</topic><topic>Synaptic Transmission - drug effects</topic><topic>Synaptic Transmission - physiology</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pflieger, Jean-François</creatorcontrib><creatorcontrib>Clarac, François</creatorcontrib><creatorcontrib>Vinay, Laurent</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Brain research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pflieger, Jean-François</au><au>Clarac, François</au><au>Vinay, Laurent</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Picrotoxin and bicuculline have different effects on lumbar spinal networks and motoneurons in the neonatal rat</atitle><jtitle>Brain research</jtitle><addtitle>Brain Res</addtitle><date>2002-05-10</date><risdate>2002</risdate><volume>935</volume><issue>1</issue><spage>81</spage><epage>86</epage><pages>81-86</pages><issn>0006-8993</issn><eissn>1872-6240</eissn><coden>BRREAP</coden><abstract>Bicuculline is the most commonly used GABA
A receptor antagonist to investigate the contribution of these receptors in motor control. However, this compound has been shown recently to potentiate the burst firing of neurons in various brain regions by blocking a calcium-activated potassium current underlying the spike after-hyperpolarization (AHP). This effect may distort our understanding of the role of GABA
A receptors at the network level. In vitro brainstem-spinal cord preparations isolated from neonatal rats were used to compare the effects of bicuculline methiodide (bicuculline-M) and picrotoxin (PTX), another GABA
A receptor antagonist, on the AHP of lumbar motoneurons as well as on spontaneous and locomotor-like motor activities. Intracellular recordings of lumbar motoneurons showed that bicuculline-M (20 μM) reduced the AHP to 57% of control whereas PTX (20–60 μM) had no significant effect. Bath-application of increasing concentrations of PTX caused an increase in spontaneous ventral root activity, which further increased significantly when bicuculline-M was added. The effects of both antagonists were tested on fictive locomotion. The left–right alternation was disrupted in the presence of bicuculline-M. A slow synchronous bursting activity of large amplitude also appeared in the presence of PTX. This slow rhythm was superimposed on a faster rhythm which still exhibited some degree of left–right alternation. These data demonstrate that bicuculline-M may not reveal accurately the contribution of GABA
A receptors in motor control and the intrinsic properties of disinhibited networks.</abstract><cop>London</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>12062476</pmid><doi>10.1016/S0006-8993(02)02469-1</doi><tpages>6</tpages></addata></record> |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | 2-Amino-5-phosphonovalerate - pharmacology 6-Cyano-7-nitroquinoxaline-2,3-dione - pharmacology Action Potentials - drug effects Action Potentials - physiology Animals Animals, Newborn Bicuculline Bicuculline - pharmacology Biological and medical sciences Central pattern generator Disinhibition Excitatory Amino Acid Antagonists Fundamental and applied biological sciences. Psychology GABA A receptor GABA Antagonists - pharmacology GABA-A Receptor Antagonists gamma-Aminobutyric Acid - metabolism Interneurons - cytology Interneurons - drug effects Interneurons - metabolism Locomotion Motor control and motor pathways. Reflexes. Control centers of vegetative functions. Vestibular system and equilibration Motor Neurons - cytology Motor Neurons - drug effects Motor Neurons - metabolism Nerve Net - cytology Nerve Net - drug effects Nerve Net - metabolism Neural Inhibition - drug effects Neural Inhibition - physiology Neural Pathways - cytology Neural Pathways - drug effects Neural Pathways - metabolism Picrotoxin - pharmacology Rats Rats, Wistar Receptors, GABA-A - metabolism Spinal Cord - cytology Spinal Cord - drug effects Spinal Cord - metabolism Synaptic Transmission - drug effects Synaptic Transmission - physiology Vertebrates: nervous system and sense organs |
| title | Picrotoxin and bicuculline have different effects on lumbar spinal networks and motoneurons in the neonatal rat |
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