Flow-Injection Assay of Catalase Activity
A novel flow-injection assay (FIA) system with a double line for catalase activity was constructed in which an oxidase is immobilized and the substrate is continuously pumped to reduce the dissolved oxygen and to generate a given level of hydrogen peroxide. The catalase in a sample decomposed the hy...
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| Veröffentlicht in: | Analytical Sciences 2004, Vol.20(3), pp.471-474 |
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| container_title | Analytical Sciences |
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| creator | UKEDA, Hiroyuki ADACHI, Yukiko SAWAMURA, Masayoshi |
| description | A novel flow-injection assay (FIA) system with a double line for catalase activity was constructed in which an oxidase is immobilized and the substrate is continuously pumped to reduce the dissolved oxygen and to generate a given level of hydrogen peroxide. The catalase in a sample decomposed the hydrogen peroxide, and thus the increase in dissolved oxygen dependent on the activity was amperometrically monitored using a Clark-type oxygen electrode. Among the examined several oxidases, uricase was most suitable for the continuous formation of hydrogen peroxide from a consideration of the stability and the conversion efficiency. Under the optimum conditions, a linear calibration curve was obtained in the range from 21 to 210 units/mg and the reproducibility (CV) was better than 2% by 35 successive determinations of 210 units/ml catalase preparation. The sampling frequency was about 15 samples/h. The present FIA system was applicable to monitor the inactivation of catalase by glycation. |
| doi_str_mv | 10.2116/analsci.20.471 |
| format | Article |
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The catalase in a sample decomposed the hydrogen peroxide, and thus the increase in dissolved oxygen dependent on the activity was amperometrically monitored using a Clark-type oxygen electrode. Among the examined several oxidases, uricase was most suitable for the continuous formation of hydrogen peroxide from a consideration of the stability and the conversion efficiency. Under the optimum conditions, a linear calibration curve was obtained in the range from 21 to 210 units/mg and the reproducibility (CV) was better than 2% by 35 successive determinations of 210 units/ml catalase preparation. The sampling frequency was about 15 samples/h. The present FIA system was applicable to monitor the inactivation of catalase by glycation.</description><identifier>ISSN: 0910-6340</identifier><identifier>EISSN: 1348-2246</identifier><identifier>DOI: 10.2116/analsci.20.471</identifier><identifier>PMID: 15068290</identifier><language>eng</language><publisher>Japan: The Japan Society for Analytical Chemistry</publisher><subject>Calibration ; Catalase - analysis ; Enzymes, Immobilized ; Flow Injection Analysis ; Hydrogen Peroxide - chemistry ; Indicators and Reagents ; Oxygen - analysis</subject><ispartof>Analytical Sciences, 2004, Vol.20(3), pp.471-474</ispartof><rights>2004 by The Japan Society for Analytical Chemistry</rights><rights>Copyright Japan Science and Technology Agency 2004</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c571t-e6741f924a2e89d0b9b235f9c92dabcf19a22ff0fe825ff047ad4d37ca2587f83</citedby><cites>FETCH-LOGICAL-c571t-e6741f924a2e89d0b9b235f9c92dabcf19a22ff0fe825ff047ad4d37ca2587f83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1877,4010,27900,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15068290$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>UKEDA, Hiroyuki</creatorcontrib><creatorcontrib>ADACHI, Yukiko</creatorcontrib><creatorcontrib>SAWAMURA, Masayoshi</creatorcontrib><title>Flow-Injection Assay of Catalase Activity</title><title>Analytical Sciences</title><addtitle>Anal Sci</addtitle><description>A novel flow-injection assay (FIA) system with a double line for catalase activity was constructed in which an oxidase is immobilized and the substrate is continuously pumped to reduce the dissolved oxygen and to generate a given level of hydrogen peroxide. The catalase in a sample decomposed the hydrogen peroxide, and thus the increase in dissolved oxygen dependent on the activity was amperometrically monitored using a Clark-type oxygen electrode. Among the examined several oxidases, uricase was most suitable for the continuous formation of hydrogen peroxide from a consideration of the stability and the conversion efficiency. Under the optimum conditions, a linear calibration curve was obtained in the range from 21 to 210 units/mg and the reproducibility (CV) was better than 2% by 35 successive determinations of 210 units/ml catalase preparation. The sampling frequency was about 15 samples/h. The present FIA system was applicable to monitor the inactivation of catalase by glycation.</description><subject>Calibration</subject><subject>Catalase - analysis</subject><subject>Enzymes, Immobilized</subject><subject>Flow Injection Analysis</subject><subject>Hydrogen Peroxide - chemistry</subject><subject>Indicators and Reagents</subject><subject>Oxygen - analysis</subject><issn>0910-6340</issn><issn>1348-2246</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkMFLwzAUh4Mobk6vHqUgCB5ak9e0TY6jOB0MvOg5pGmiLV07k1bZf29GyxQv7x3e936P9yF0TXAEhKQPspWNU1UEOKIZOUFzElMWAtD0FM0xJzhMY4pn6MK5GmMCDOAczUiCUwYcz9H9qum-w3Vba9VXXRssnZP7oDNBLnvZSKeDpR98Vf3-Ep0Zf0tfTX2B3laPr_lzuHl5WufLTaiSjPShTjNKDAcqQTNe4oIXECeGKw6lLJQhXAIYg41mkPhOM1nSMs6UhIRlhsULdDfm7mz3OWjXi23llG4a2epucCIjjID_3YO3_8C6G-zBhyCUsoSyOMGeikZK2c45q43Y2Wor7V4QLA4KxaRQABZeoV-4mWKHYqvLX3xy5oF8BGrXy3d9BKTtK9Xov3nxWHzscao-pBW6jX8AhG-Elw</recordid><startdate>2004</startdate><enddate>2004</enddate><creator>UKEDA, Hiroyuki</creator><creator>ADACHI, Yukiko</creator><creator>SAWAMURA, Masayoshi</creator><general>The Japan Society for Analytical Chemistry</general><general>Japan Science and Technology Agency</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SE</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>FR3</scope><scope>H8G</scope><scope>JG9</scope><scope>L7M</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>2004</creationdate><title>Flow-Injection Assay of Catalase Activity</title><author>UKEDA, Hiroyuki ; ADACHI, Yukiko ; SAWAMURA, Masayoshi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c571t-e6741f924a2e89d0b9b235f9c92dabcf19a22ff0fe825ff047ad4d37ca2587f83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Calibration</topic><topic>Catalase - analysis</topic><topic>Enzymes, Immobilized</topic><topic>Flow Injection Analysis</topic><topic>Hydrogen Peroxide - chemistry</topic><topic>Indicators and Reagents</topic><topic>Oxygen - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>UKEDA, Hiroyuki</creatorcontrib><creatorcontrib>ADACHI, Yukiko</creatorcontrib><creatorcontrib>SAWAMURA, Masayoshi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Copper Technical Reference Library</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical Sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>UKEDA, Hiroyuki</au><au>ADACHI, Yukiko</au><au>SAWAMURA, Masayoshi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Flow-Injection Assay of Catalase Activity</atitle><jtitle>Analytical Sciences</jtitle><addtitle>Anal Sci</addtitle><date>2004</date><risdate>2004</risdate><volume>20</volume><issue>3</issue><spage>471</spage><epage>474</epage><pages>471-474</pages><issn>0910-6340</issn><eissn>1348-2246</eissn><abstract>A novel flow-injection assay (FIA) system with a double line for catalase activity was constructed in which an oxidase is immobilized and the substrate is continuously pumped to reduce the dissolved oxygen and to generate a given level of hydrogen peroxide. The catalase in a sample decomposed the hydrogen peroxide, and thus the increase in dissolved oxygen dependent on the activity was amperometrically monitored using a Clark-type oxygen electrode. Among the examined several oxidases, uricase was most suitable for the continuous formation of hydrogen peroxide from a consideration of the stability and the conversion efficiency. Under the optimum conditions, a linear calibration curve was obtained in the range from 21 to 210 units/mg and the reproducibility (CV) was better than 2% by 35 successive determinations of 210 units/ml catalase preparation. The sampling frequency was about 15 samples/h. The present FIA system was applicable to monitor the inactivation of catalase by glycation.</abstract><cop>Japan</cop><pub>The Japan Society for Analytical Chemistry</pub><pmid>15068290</pmid><doi>10.2116/analsci.20.471</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Analytical Sciences, 2004, Vol.20(3), pp.471-474 |
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| language | eng |
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| source | MEDLINE; J-STAGE (Japan Science & Technology Information Aggregator, Electronic) Freely Available Titles - Japanese; Open Access Titles of Japan; SpringerLink Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Free Full-Text Journals in Chemistry |
| subjects | Calibration Catalase - analysis Enzymes, Immobilized Flow Injection Analysis Hydrogen Peroxide - chemistry Indicators and Reagents Oxygen - analysis |
| title | Flow-Injection Assay of Catalase Activity |
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