Rhythmic expression of DEC1 and DEC2 in peripheral tissues: DEC2 is a potent suppressor for hepatic cytochrome P450s opposing DBP
The mammalian master molecular clock consisting of several clock gene products in the suprachiasmatic nucleus (SCN) drives circadian rhythms in behaviour and physiology. Molecular clocks consisting of the same components also exist in various peripheral organs. DEC1 and DEC2, basic helix-loop-helix...
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Veröffentlicht in: | Genes to cells : devoted to molecular & cellular mechanisms 2004-04, Vol.9 (4), p.317-329 |
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creator | Noshiro, Mitsuhide Kawamoto, Takeshi Furukawa, Masae Fujimoto, Katsumi Yoshida, Yuzo Sasabe, Eri Tsutsumi, Shinichi Hamada, Taizo Honma, Sato Honma, Ken‐ichi Kato, Yukio |
description | The mammalian master molecular clock consisting of several clock gene products in the suprachiasmatic nucleus (SCN) drives circadian rhythms in behaviour and physiology. Molecular clocks consisting of the same components also exist in various peripheral organs. DEC1 and DEC2, basic helix-loop-helix transcription factors, were recently reported to be involved in the central clock in the SCN. We examined the expression profile of DEC1 and DEC2 in the periphery and their roles in the regulation of oscillating target genes in the liver. Levels of DEC1 and DEC2 mRNA exhibited a day-night variation in various peripheral tissues of rats. In the liver, their expression was high during the subjective night. Transfection assays showed that DEC2, but not DEC1, suppressed the transcription of the cholesterol 7alpha-hydroxylase gene (CYP7A), overwhelming the potent enhancement by D-site binding protein (DBP). Electrophoretic mobility shift assays indicated that DEC2 binds to the E-box (CACATG) at the -219/-214 region of CYP7A. The transcriptional activities of the other sterol metabolizing cytochrome P450s (Cyps), CYP8B and CYP51, were also suppressed by DEC2 but not DEC1. DEC2, but not DEC1, works as a direct output mediator that transmits the circadian signals to the hepatic functions, including the CYP7A, CYP8B, and CYP51 expression. |
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Molecular clocks consisting of the same components also exist in various peripheral organs. DEC1 and DEC2, basic helix-loop-helix transcription factors, were recently reported to be involved in the central clock in the SCN. We examined the expression profile of DEC1 and DEC2 in the periphery and their roles in the regulation of oscillating target genes in the liver. Levels of DEC1 and DEC2 mRNA exhibited a day-night variation in various peripheral tissues of rats. In the liver, their expression was high during the subjective night. Transfection assays showed that DEC2, but not DEC1, suppressed the transcription of the cholesterol 7alpha-hydroxylase gene (CYP7A), overwhelming the potent enhancement by D-site binding protein (DBP). Electrophoretic mobility shift assays indicated that DEC2 binds to the E-box (CACATG) at the -219/-214 region of CYP7A. The transcriptional activities of the other sterol metabolizing cytochrome P450s (Cyps), CYP8B and CYP51, were also suppressed by DEC2 but not DEC1. DEC2, but not DEC1, works as a direct output mediator that transmits the circadian signals to the hepatic functions, including the CYP7A, CYP8B, and CYP51 expression.</description><identifier>ISSN: 1356-9597</identifier><identifier>EISSN: 1365-2443</identifier><identifier>DOI: 10.1111/j.1356-9597.2004.00722.x</identifier><identifier>PMID: 15066123</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing, Ltd</publisher><subject>Animals ; Basic Helix-Loop-Helix Transcription Factors ; Cholesterol 7-alpha-Hydroxylase - metabolism ; Circadian Rhythm - physiology ; Cytochrome P-450 Enzyme System - metabolism ; DNA-Binding Proteins - metabolism ; Liver - metabolism ; Rats ; RNA, Messenger - metabolism ; Steroid 12-alpha-Hydroxylase - metabolism ; Suprachiasmatic Nucleus - metabolism ; Transcription Factors - genetics ; Transcription Factors - metabolism ; Tumor Suppressor Proteins - genetics ; Tumor Suppressor Proteins - metabolism</subject><ispartof>Genes to cells : devoted to molecular & cellular mechanisms, 2004-04, Vol.9 (4), p.317-329</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4632-647b2b4166fd21b722d0a0c9b942ea67d5c6bc58d7a8acb26b443a33e2eaafdb3</citedby><cites>FETCH-LOGICAL-c4632-647b2b4166fd21b722d0a0c9b942ea67d5c6bc58d7a8acb26b443a33e2eaafdb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1356-9597.2004.00722.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1356-9597.2004.00722.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,1433,27924,27925,45574,45575,46409,46833</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15066123$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Noshiro, Mitsuhide</creatorcontrib><creatorcontrib>Kawamoto, Takeshi</creatorcontrib><creatorcontrib>Furukawa, Masae</creatorcontrib><creatorcontrib>Fujimoto, Katsumi</creatorcontrib><creatorcontrib>Yoshida, Yuzo</creatorcontrib><creatorcontrib>Sasabe, Eri</creatorcontrib><creatorcontrib>Tsutsumi, Shinichi</creatorcontrib><creatorcontrib>Hamada, Taizo</creatorcontrib><creatorcontrib>Honma, Sato</creatorcontrib><creatorcontrib>Honma, Ken‐ichi</creatorcontrib><creatorcontrib>Kato, Yukio</creatorcontrib><title>Rhythmic expression of DEC1 and DEC2 in peripheral tissues: DEC2 is a potent suppressor for hepatic cytochrome P450s opposing DBP</title><title>Genes to cells : devoted to molecular & cellular mechanisms</title><addtitle>Genes Cells</addtitle><description>The mammalian master molecular clock consisting of several clock gene products in the suprachiasmatic nucleus (SCN) drives circadian rhythms in behaviour and physiology. Molecular clocks consisting of the same components also exist in various peripheral organs. DEC1 and DEC2, basic helix-loop-helix transcription factors, were recently reported to be involved in the central clock in the SCN. We examined the expression profile of DEC1 and DEC2 in the periphery and their roles in the regulation of oscillating target genes in the liver. Levels of DEC1 and DEC2 mRNA exhibited a day-night variation in various peripheral tissues of rats. In the liver, their expression was high during the subjective night. Transfection assays showed that DEC2, but not DEC1, suppressed the transcription of the cholesterol 7alpha-hydroxylase gene (CYP7A), overwhelming the potent enhancement by D-site binding protein (DBP). Electrophoretic mobility shift assays indicated that DEC2 binds to the E-box (CACATG) at the -219/-214 region of CYP7A. The transcriptional activities of the other sterol metabolizing cytochrome P450s (Cyps), CYP8B and CYP51, were also suppressed by DEC2 but not DEC1. DEC2, but not DEC1, works as a direct output mediator that transmits the circadian signals to the hepatic functions, including the CYP7A, CYP8B, and CYP51 expression.</description><subject>Animals</subject><subject>Basic Helix-Loop-Helix Transcription Factors</subject><subject>Cholesterol 7-alpha-Hydroxylase - metabolism</subject><subject>Circadian Rhythm - physiology</subject><subject>Cytochrome P-450 Enzyme System - metabolism</subject><subject>DNA-Binding Proteins - metabolism</subject><subject>Liver - metabolism</subject><subject>Rats</subject><subject>RNA, Messenger - metabolism</subject><subject>Steroid 12-alpha-Hydroxylase - metabolism</subject><subject>Suprachiasmatic Nucleus - metabolism</subject><subject>Transcription Factors - genetics</subject><subject>Transcription Factors - metabolism</subject><subject>Tumor Suppressor Proteins - genetics</subject><subject>Tumor Suppressor Proteins - metabolism</subject><issn>1356-9597</issn><issn>1365-2443</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkV9v2yAUxdG0amm7fYWJp77Z44_B8bSXLenSSpVaTd0zAoxnItswcNTksd-8OLHUxxYJcaT7u-cCBwCIUY7T-rbNMWU8q1hV5gShIkeoJCTffwDnmHKWkaKgHyc9QwtwEeMWIUwJYp_AAjPEOSb0HDz_aQ9j21sNzd4HE6N1A3QNXF-vMJRDPQkC7QC9Cda3JsgOjjbGnYnf51qEEno3mmGEceePJi7AJu3WeDkma30YnW6D6w18KBiK0Hnvoh3-wfWvh8_grJFdNF_m8xL8_X39uLrJ7u43t6ufd5kuOCUZL0pFVIE5b2qCVXpujSTSlaoKYiQva6a50mxZl3IptSJcpT-QlJpUlU2t6CW4Ovn64P6n64-it1GbrpODcbsoSrzEiJTsTRCXFeOU4gQuT6AOLsZgGuGD7WU4CIzElJPYiikCMUUgppzEMSexT61f5xk71Zv6tXEOJgE_TsCT7czh3cZi87hKgr4AZK-g3w</recordid><startdate>200404</startdate><enddate>200404</enddate><creator>Noshiro, Mitsuhide</creator><creator>Kawamoto, Takeshi</creator><creator>Furukawa, Masae</creator><creator>Fujimoto, Katsumi</creator><creator>Yoshida, Yuzo</creator><creator>Sasabe, Eri</creator><creator>Tsutsumi, Shinichi</creator><creator>Hamada, Taizo</creator><creator>Honma, Sato</creator><creator>Honma, Ken‐ichi</creator><creator>Kato, Yukio</creator><general>Blackwell Publishing, Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>200404</creationdate><title>Rhythmic expression of DEC1 and DEC2 in peripheral tissues: DEC2 is a potent suppressor for hepatic cytochrome P450s opposing DBP</title><author>Noshiro, Mitsuhide ; Kawamoto, Takeshi ; Furukawa, Masae ; Fujimoto, Katsumi ; Yoshida, Yuzo ; Sasabe, Eri ; Tsutsumi, Shinichi ; Hamada, Taizo ; Honma, Sato ; Honma, Ken‐ichi ; Kato, Yukio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4632-647b2b4166fd21b722d0a0c9b942ea67d5c6bc58d7a8acb26b443a33e2eaafdb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Animals</topic><topic>Basic Helix-Loop-Helix Transcription Factors</topic><topic>Cholesterol 7-alpha-Hydroxylase - metabolism</topic><topic>Circadian Rhythm - physiology</topic><topic>Cytochrome P-450 Enzyme System - metabolism</topic><topic>DNA-Binding Proteins - metabolism</topic><topic>Liver - metabolism</topic><topic>Rats</topic><topic>RNA, Messenger - metabolism</topic><topic>Steroid 12-alpha-Hydroxylase - metabolism</topic><topic>Suprachiasmatic Nucleus - metabolism</topic><topic>Transcription Factors - genetics</topic><topic>Transcription Factors - metabolism</topic><topic>Tumor Suppressor Proteins - genetics</topic><topic>Tumor Suppressor Proteins - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Noshiro, Mitsuhide</creatorcontrib><creatorcontrib>Kawamoto, Takeshi</creatorcontrib><creatorcontrib>Furukawa, Masae</creatorcontrib><creatorcontrib>Fujimoto, Katsumi</creatorcontrib><creatorcontrib>Yoshida, Yuzo</creatorcontrib><creatorcontrib>Sasabe, Eri</creatorcontrib><creatorcontrib>Tsutsumi, Shinichi</creatorcontrib><creatorcontrib>Hamada, Taizo</creatorcontrib><creatorcontrib>Honma, Sato</creatorcontrib><creatorcontrib>Honma, Ken‐ichi</creatorcontrib><creatorcontrib>Kato, Yukio</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Genes to cells : devoted to molecular & cellular mechanisms</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Noshiro, Mitsuhide</au><au>Kawamoto, Takeshi</au><au>Furukawa, Masae</au><au>Fujimoto, Katsumi</au><au>Yoshida, Yuzo</au><au>Sasabe, Eri</au><au>Tsutsumi, Shinichi</au><au>Hamada, Taizo</au><au>Honma, Sato</au><au>Honma, Ken‐ichi</au><au>Kato, Yukio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rhythmic expression of DEC1 and DEC2 in peripheral tissues: DEC2 is a potent suppressor for hepatic cytochrome P450s opposing DBP</atitle><jtitle>Genes to cells : devoted to molecular & cellular mechanisms</jtitle><addtitle>Genes Cells</addtitle><date>2004-04</date><risdate>2004</risdate><volume>9</volume><issue>4</issue><spage>317</spage><epage>329</epage><pages>317-329</pages><issn>1356-9597</issn><eissn>1365-2443</eissn><abstract>The mammalian master molecular clock consisting of several clock gene products in the suprachiasmatic nucleus (SCN) drives circadian rhythms in behaviour and physiology. Molecular clocks consisting of the same components also exist in various peripheral organs. DEC1 and DEC2, basic helix-loop-helix transcription factors, were recently reported to be involved in the central clock in the SCN. We examined the expression profile of DEC1 and DEC2 in the periphery and their roles in the regulation of oscillating target genes in the liver. Levels of DEC1 and DEC2 mRNA exhibited a day-night variation in various peripheral tissues of rats. In the liver, their expression was high during the subjective night. Transfection assays showed that DEC2, but not DEC1, suppressed the transcription of the cholesterol 7alpha-hydroxylase gene (CYP7A), overwhelming the potent enhancement by D-site binding protein (DBP). Electrophoretic mobility shift assays indicated that DEC2 binds to the E-box (CACATG) at the -219/-214 region of CYP7A. The transcriptional activities of the other sterol metabolizing cytochrome P450s (Cyps), CYP8B and CYP51, were also suppressed by DEC2 but not DEC1. DEC2, but not DEC1, works as a direct output mediator that transmits the circadian signals to the hepatic functions, including the CYP7A, CYP8B, and CYP51 expression.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing, Ltd</pub><pmid>15066123</pmid><doi>10.1111/j.1356-9597.2004.00722.x</doi><tpages>13</tpages></addata></record> |
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subjects | Animals Basic Helix-Loop-Helix Transcription Factors Cholesterol 7-alpha-Hydroxylase - metabolism Circadian Rhythm - physiology Cytochrome P-450 Enzyme System - metabolism DNA-Binding Proteins - metabolism Liver - metabolism Rats RNA, Messenger - metabolism Steroid 12-alpha-Hydroxylase - metabolism Suprachiasmatic Nucleus - metabolism Transcription Factors - genetics Transcription Factors - metabolism Tumor Suppressor Proteins - genetics Tumor Suppressor Proteins - metabolism |
title | Rhythmic expression of DEC1 and DEC2 in peripheral tissues: DEC2 is a potent suppressor for hepatic cytochrome P450s opposing DBP |
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