Detection of Shiga toxins, intimin and enterohemolysin in Escherichia coli strains isolated from children in Eastern Slovakia
Fifty Escherichia coli strains isolated from stool samples of 51 healthy children, 143 strains isolated from stool samples of 327 children with diarrhea and 24 strains isolated from stool samples of 21 children with suspected hemolytic uremic syndrome were examined for the presence of Shiga toxin-pr...
Gespeichert in:
| Veröffentlicht in: | Folia microbiologica 2002, Vol.47 (2), p.185-188 |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 188 |
|---|---|
| container_issue | 2 |
| container_start_page | 185 |
| container_title | Folia microbiologica |
| container_volume | 47 |
| creator | Liptákova, A Siegfried, L Podracká, L Sabol, M Sehnálková, H Bogyiová, E Rosocha, J Kmeťová, M Kerestešová, H Kotulová, D |
| description | Fifty Escherichia coli strains isolated from stool samples of 51 healthy children, 143 strains isolated from stool samples of 327 children with diarrhea and 24 strains isolated from stool samples of 21 children with suspected hemolytic uremic syndrome were examined for the presence of Shiga toxin-producing E. coli virulence factors (shiga toxin 1 and 2, intimin and enterohemolysin) and their genes. Vero-cell assay and latex agglutination were used for detection of Shiga toxin 1 and 2, TSB agar with washed erythrocytes was used for detection of enterohemolysin; genes encoding shiga toxin 1 and 2, intimin and enterohemolysin were detected using multiplex PCR. The presence ofE. coli strains harboring genes encoding shiga toxin 1 and 2 (12 strains), intimin (34 strains) and enterohemolysin (12 strains) was demonstrated. |
| doi_str_mv | 10.1007/BF02817680 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_71808755</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>71808755</sourcerecordid><originalsourceid>FETCH-LOGICAL-f261t-1668724e78c9d4cf66f9f28572aabc929204067dc93870210cb88a8aa82f5f7f3</originalsourceid><addsrcrecordid>eNpFkU1vFDEMhiMEotvChR8AudATU5zM5GOOpbRQqRKHpeeRN5N0A5mkJNmKHvjvBO2iSpYs2c_7HGxC3jA4YwDq46cr4JopqeEZWTGthm7shXxOVgBMdEL2_Igcl_IDQMLQ85fkiHEQegBYkT-fbbWm-hRpcnS99XdIa_rtY_lAfax-8ZFinKmN1ea0tUsKj6XNWl0Ws7XZm61HalLwtNSMLUh9SQGrnanLaaFtH-Zs9xEsTRPpOqQH_OnxFXnhMBT7-tBPyO3V5feLr93Nty_XF-c3neOS1Y5JqRUfrNJmnAfjpHSj41oojrgxIx85DCDVbMZeK-AMzEZr1IiaO-GU60_I6d57n9OvnS11WnwxNgSMNu3KpJgGrYRo4NsDuNssdp7us18wP07_D9aA9wcAi8HgMkbjyxM3MK1H9U_0bs85TBPe5cbcrjkwCe0LYz-I_i827oNa</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>71808755</pqid></control><display><type>article</type><title>Detection of Shiga toxins, intimin and enterohemolysin in Escherichia coli strains isolated from children in Eastern Slovakia</title><source>MEDLINE</source><source>SpringerNature Journals</source><creator>Liptákova, A ; Siegfried, L ; Podracká, L ; Sabol, M ; Sehnálková, H ; Bogyiová, E ; Rosocha, J ; Kmeťová, M ; Kerestešová, H ; Kotulová, D</creator><creatorcontrib>Liptákova, A ; Siegfried, L ; Podracká, L ; Sabol, M ; Sehnálková, H ; Bogyiová, E ; Rosocha, J ; Kmeťová, M ; Kerestešová, H ; Kotulová, D</creatorcontrib><description>Fifty Escherichia coli strains isolated from stool samples of 51 healthy children, 143 strains isolated from stool samples of 327 children with diarrhea and 24 strains isolated from stool samples of 21 children with suspected hemolytic uremic syndrome were examined for the presence of Shiga toxin-producing E. coli virulence factors (shiga toxin 1 and 2, intimin and enterohemolysin) and their genes. Vero-cell assay and latex agglutination were used for detection of Shiga toxin 1 and 2, TSB agar with washed erythrocytes was used for detection of enterohemolysin; genes encoding shiga toxin 1 and 2, intimin and enterohemolysin were detected using multiplex PCR. The presence ofE. coli strains harboring genes encoding shiga toxin 1 and 2 (12 strains), intimin (34 strains) and enterohemolysin (12 strains) was demonstrated.</description><identifier>ISSN: 0015-5632</identifier><identifier>EISSN: 1874-9356</identifier><identifier>DOI: 10.1007/BF02817680</identifier><identifier>PMID: 12058400</identifier><identifier>CODEN: FOMIAZ</identifier><language>eng</language><publisher>Praha: Springer Netherlands</publisher><subject>Adhesins, Bacterial - analysis ; agar ; agglutination ; Bacterial diseases ; Bacterial diseases of the urinary system ; Biological and medical sciences ; Carrier Proteins - analysis ; Child ; Child, Preschool ; children ; diarrhea ; erythrocytes ; Escherichia coli - chemistry ; Escherichia coli - isolation & purification ; Escherichia coli Infections - microbiology ; Escherichia coli Proteins ; feces ; Feces - microbiology ; genes ; Hemolysin Proteins - analysis ; hemolytic uremic syndrome ; Human bacterial diseases ; Humans ; Infectious diseases ; intimin ; latex ; Medical sciences ; Polymerase Chain Reaction ; Shiga Toxins - analysis ; Shiga-like toxin 1 ; Slovakia ; virulence</subject><ispartof>Folia microbiologica, 2002, Vol.47 (2), p.185-188</ispartof><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4024,27923,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14188975$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12058400$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liptákova, A</creatorcontrib><creatorcontrib>Siegfried, L</creatorcontrib><creatorcontrib>Podracká, L</creatorcontrib><creatorcontrib>Sabol, M</creatorcontrib><creatorcontrib>Sehnálková, H</creatorcontrib><creatorcontrib>Bogyiová, E</creatorcontrib><creatorcontrib>Rosocha, J</creatorcontrib><creatorcontrib>Kmeťová, M</creatorcontrib><creatorcontrib>Kerestešová, H</creatorcontrib><creatorcontrib>Kotulová, D</creatorcontrib><title>Detection of Shiga toxins, intimin and enterohemolysin in Escherichia coli strains isolated from children in Eastern Slovakia</title><title>Folia microbiologica</title><addtitle>Folia Microbiol (Praha)</addtitle><description>Fifty Escherichia coli strains isolated from stool samples of 51 healthy children, 143 strains isolated from stool samples of 327 children with diarrhea and 24 strains isolated from stool samples of 21 children with suspected hemolytic uremic syndrome were examined for the presence of Shiga toxin-producing E. coli virulence factors (shiga toxin 1 and 2, intimin and enterohemolysin) and their genes. Vero-cell assay and latex agglutination were used for detection of Shiga toxin 1 and 2, TSB agar with washed erythrocytes was used for detection of enterohemolysin; genes encoding shiga toxin 1 and 2, intimin and enterohemolysin were detected using multiplex PCR. The presence ofE. coli strains harboring genes encoding shiga toxin 1 and 2 (12 strains), intimin (34 strains) and enterohemolysin (12 strains) was demonstrated.</description><subject>Adhesins, Bacterial - analysis</subject><subject>agar</subject><subject>agglutination</subject><subject>Bacterial diseases</subject><subject>Bacterial diseases of the urinary system</subject><subject>Biological and medical sciences</subject><subject>Carrier Proteins - analysis</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>children</subject><subject>diarrhea</subject><subject>erythrocytes</subject><subject>Escherichia coli - chemistry</subject><subject>Escherichia coli - isolation & purification</subject><subject>Escherichia coli Infections - microbiology</subject><subject>Escherichia coli Proteins</subject><subject>feces</subject><subject>Feces - microbiology</subject><subject>genes</subject><subject>Hemolysin Proteins - analysis</subject><subject>hemolytic uremic syndrome</subject><subject>Human bacterial diseases</subject><subject>Humans</subject><subject>Infectious diseases</subject><subject>intimin</subject><subject>latex</subject><subject>Medical sciences</subject><subject>Polymerase Chain Reaction</subject><subject>Shiga Toxins - analysis</subject><subject>Shiga-like toxin 1</subject><subject>Slovakia</subject><subject>virulence</subject><issn>0015-5632</issn><issn>1874-9356</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkU1vFDEMhiMEotvChR8AudATU5zM5GOOpbRQqRKHpeeRN5N0A5mkJNmKHvjvBO2iSpYs2c_7HGxC3jA4YwDq46cr4JopqeEZWTGthm7shXxOVgBMdEL2_Igcl_IDQMLQ85fkiHEQegBYkT-fbbWm-hRpcnS99XdIa_rtY_lAfax-8ZFinKmN1ea0tUsKj6XNWl0Ws7XZm61HalLwtNSMLUh9SQGrnanLaaFtH-Zs9xEsTRPpOqQH_OnxFXnhMBT7-tBPyO3V5feLr93Nty_XF-c3neOS1Y5JqRUfrNJmnAfjpHSj41oojrgxIx85DCDVbMZeK-AMzEZr1IiaO-GU60_I6d57n9OvnS11WnwxNgSMNu3KpJgGrYRo4NsDuNssdp7us18wP07_D9aA9wcAi8HgMkbjyxM3MK1H9U_0bs85TBPe5cbcrjkwCe0LYz-I_i827oNa</recordid><startdate>2002</startdate><enddate>2002</enddate><creator>Liptákova, A</creator><creator>Siegfried, L</creator><creator>Podracká, L</creator><creator>Sabol, M</creator><creator>Sehnálková, H</creator><creator>Bogyiová, E</creator><creator>Rosocha, J</creator><creator>Kmeťová, M</creator><creator>Kerestešová, H</creator><creator>Kotulová, D</creator><general>Springer Netherlands</general><general>Academia</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>2002</creationdate><title>Detection of Shiga toxins, intimin and enterohemolysin in Escherichia coli strains isolated from children in Eastern Slovakia</title><author>Liptákova, A ; Siegfried, L ; Podracká, L ; Sabol, M ; Sehnálková, H ; Bogyiová, E ; Rosocha, J ; Kmeťová, M ; Kerestešová, H ; Kotulová, D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-f261t-1668724e78c9d4cf66f9f28572aabc929204067dc93870210cb88a8aa82f5f7f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Adhesins, Bacterial - analysis</topic><topic>agar</topic><topic>agglutination</topic><topic>Bacterial diseases</topic><topic>Bacterial diseases of the urinary system</topic><topic>Biological and medical sciences</topic><topic>Carrier Proteins - analysis</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>children</topic><topic>diarrhea</topic><topic>erythrocytes</topic><topic>Escherichia coli - chemistry</topic><topic>Escherichia coli - isolation & purification</topic><topic>Escherichia coli Infections - microbiology</topic><topic>Escherichia coli Proteins</topic><topic>feces</topic><topic>Feces - microbiology</topic><topic>genes</topic><topic>Hemolysin Proteins - analysis</topic><topic>hemolytic uremic syndrome</topic><topic>Human bacterial diseases</topic><topic>Humans</topic><topic>Infectious diseases</topic><topic>intimin</topic><topic>latex</topic><topic>Medical sciences</topic><topic>Polymerase Chain Reaction</topic><topic>Shiga Toxins - analysis</topic><topic>Shiga-like toxin 1</topic><topic>Slovakia</topic><topic>virulence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liptákova, A</creatorcontrib><creatorcontrib>Siegfried, L</creatorcontrib><creatorcontrib>Podracká, L</creatorcontrib><creatorcontrib>Sabol, M</creatorcontrib><creatorcontrib>Sehnálková, H</creatorcontrib><creatorcontrib>Bogyiová, E</creatorcontrib><creatorcontrib>Rosocha, J</creatorcontrib><creatorcontrib>Kmeťová, M</creatorcontrib><creatorcontrib>Kerestešová, H</creatorcontrib><creatorcontrib>Kotulová, D</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Folia microbiologica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liptákova, A</au><au>Siegfried, L</au><au>Podracká, L</au><au>Sabol, M</au><au>Sehnálková, H</au><au>Bogyiová, E</au><au>Rosocha, J</au><au>Kmeťová, M</au><au>Kerestešová, H</au><au>Kotulová, D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of Shiga toxins, intimin and enterohemolysin in Escherichia coli strains isolated from children in Eastern Slovakia</atitle><jtitle>Folia microbiologica</jtitle><addtitle>Folia Microbiol (Praha)</addtitle><date>2002</date><risdate>2002</risdate><volume>47</volume><issue>2</issue><spage>185</spage><epage>188</epage><pages>185-188</pages><issn>0015-5632</issn><eissn>1874-9356</eissn><coden>FOMIAZ</coden><abstract>Fifty Escherichia coli strains isolated from stool samples of 51 healthy children, 143 strains isolated from stool samples of 327 children with diarrhea and 24 strains isolated from stool samples of 21 children with suspected hemolytic uremic syndrome were examined for the presence of Shiga toxin-producing E. coli virulence factors (shiga toxin 1 and 2, intimin and enterohemolysin) and their genes. Vero-cell assay and latex agglutination were used for detection of Shiga toxin 1 and 2, TSB agar with washed erythrocytes was used for detection of enterohemolysin; genes encoding shiga toxin 1 and 2, intimin and enterohemolysin were detected using multiplex PCR. The presence ofE. coli strains harboring genes encoding shiga toxin 1 and 2 (12 strains), intimin (34 strains) and enterohemolysin (12 strains) was demonstrated.</abstract><cop>Praha</cop><pub>Springer Netherlands</pub><pmid>12058400</pmid><doi>10.1007/BF02817680</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0015-5632 |
| ispartof | Folia microbiologica, 2002, Vol.47 (2), p.185-188 |
| issn | 0015-5632 1874-9356 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_71808755 |
| source | MEDLINE; SpringerNature Journals |
| subjects | Adhesins, Bacterial - analysis agar agglutination Bacterial diseases Bacterial diseases of the urinary system Biological and medical sciences Carrier Proteins - analysis Child Child, Preschool children diarrhea erythrocytes Escherichia coli - chemistry Escherichia coli - isolation & purification Escherichia coli Infections - microbiology Escherichia coli Proteins feces Feces - microbiology genes Hemolysin Proteins - analysis hemolytic uremic syndrome Human bacterial diseases Humans Infectious diseases intimin latex Medical sciences Polymerase Chain Reaction Shiga Toxins - analysis Shiga-like toxin 1 Slovakia virulence |
| title | Detection of Shiga toxins, intimin and enterohemolysin in Escherichia coli strains isolated from children in Eastern Slovakia |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T18%3A21%3A51IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Detection%20of%20Shiga%20toxins,%20intimin%20and%20enterohemolysin%20in%20Escherichia%20coli%20strains%20isolated%20from%20children%20in%20Eastern%20Slovakia&rft.jtitle=Folia%20microbiologica&rft.au=Lipt%C3%A1kova,%20A&rft.date=2002&rft.volume=47&rft.issue=2&rft.spage=185&rft.epage=188&rft.pages=185-188&rft.issn=0015-5632&rft.eissn=1874-9356&rft.coden=FOMIAZ&rft_id=info:doi/10.1007/BF02817680&rft_dat=%3Cproquest_pubme%3E71808755%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=71808755&rft_id=info:pmid/12058400&rfr_iscdi=true |