Separation of tocopherols by nano-liquid chromatography
Nanoliquid chromatography (nano-LC) was used for the separation of tocopherols (δ-, γ-, α-TOH), α-tocopherol acetate (α-TOH-Ac) and an antioxidant compound, namely butylated hydroxytoluene (BHT) used to prevent TOHs autoxidation. The separation was carried out in a fused silica capillary of 100 μm I...
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| Veröffentlicht in: | Journal of pharmaceutical and biomedical analysis 2004-04, Vol.35 (2), p.331-337 |
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| container_title | Journal of pharmaceutical and biomedical analysis |
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| creator | Fanali, Salvatore Camera, Emanuela Chankvetadze, Bezhan D’Orazio, Giovanni Quaglia, Maria Giovanna |
| description | Nanoliquid chromatography (nano-LC) was used for the separation of tocopherols (δ-, γ-, α-TOH), α-tocopherol acetate (α-TOH-Ac) and an antioxidant compound, namely butylated hydroxytoluene (BHT) used to prevent TOHs autoxidation. The separation was carried out in a fused silica capillary of 100
μm I.D. and 375
μm O.D. packed in our laboratory with RP
18 silica stationary phase of either 5- or 3-μm diameter (23-cm long). The mobile phase was composed by mixtures of methanol (MeOH), acetonitrile (MeCN) and water. Typical analyses time for the separation of all the five components of the mixture were 6–9
min depending on the composition of the mobile phase. Efficiency and resolution were strongly influenced by the particle diameter and the highest
R
s and
N/
m values were observed using 3-μm RP
18 particles. Experiments performed with capillaries packed with 3-μm RP
18 particles provided good limit of detection (LOD) and limit of quantification (LOQ) (for δ-, γ-TOH, α-TOH-Ac were 4 and 8
μg/ml, while for α-TOH were 6 and 10
μg/ml, respectively). The optimized method was applied to extracts of serum and pharmaceutical preparation containing α-TOH and α-TOH-Ac. |
| doi_str_mv | 10.1016/S0731-7085(03)00646-0 |
| format | Article |
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μm I.D. and 375
μm O.D. packed in our laboratory with RP
18 silica stationary phase of either 5- or 3-μm diameter (23-cm long). The mobile phase was composed by mixtures of methanol (MeOH), acetonitrile (MeCN) and water. Typical analyses time for the separation of all the five components of the mixture were 6–9
min depending on the composition of the mobile phase. Efficiency and resolution were strongly influenced by the particle diameter and the highest
R
s and
N/
m values were observed using 3-μm RP
18 particles. Experiments performed with capillaries packed with 3-μm RP
18 particles provided good limit of detection (LOD) and limit of quantification (LOQ) (for δ-, γ-TOH, α-TOH-Ac were 4 and 8
μg/ml, while for α-TOH were 6 and 10
μg/ml, respectively). The optimized method was applied to extracts of serum and pharmaceutical preparation containing α-TOH and α-TOH-Ac.</description><identifier>ISSN: 0731-7085</identifier><identifier>EISSN: 1873-264X</identifier><identifier>DOI: 10.1016/S0731-7085(03)00646-0</identifier><identifier>PMID: 15063466</identifier><identifier>CODEN: JPBADA</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Analysis ; Analytical, structural and metabolic biochemistry ; Biological and medical sciences ; Chromatography, Liquid - methods ; Drugs ; Fundamental and applied biological sciences. Psychology ; General pharmacology ; Medical sciences ; Nano-LC ; Nanotechnology - methods ; Pharmacology. Drug treatments ; Serum ; Tocopherols ; Tocopherols - chemistry ; Tocopherols - isolation & purification ; Vitamin E</subject><ispartof>Journal of pharmaceutical and biomedical analysis, 2004-04, Vol.35 (2), p.331-337</ispartof><rights>2003 Elsevier B.V.</rights><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c391t-fbc8a57e75df1cef5c106a0cdd7f205ab6c144ff1b12d4dca9cb44506f87f9fd3</citedby><cites>FETCH-LOGICAL-c391t-fbc8a57e75df1cef5c106a0cdd7f205ab6c144ff1b12d4dca9cb44506f87f9fd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0731-7085(03)00646-0$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>309,310,314,780,784,789,790,3550,23930,23931,25140,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15625598$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15063466$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fanali, Salvatore</creatorcontrib><creatorcontrib>Camera, Emanuela</creatorcontrib><creatorcontrib>Chankvetadze, Bezhan</creatorcontrib><creatorcontrib>D’Orazio, Giovanni</creatorcontrib><creatorcontrib>Quaglia, Maria Giovanna</creatorcontrib><title>Separation of tocopherols by nano-liquid chromatography</title><title>Journal of pharmaceutical and biomedical analysis</title><addtitle>J Pharm Biomed Anal</addtitle><description>Nanoliquid chromatography (nano-LC) was used for the separation of tocopherols (δ-, γ-, α-TOH), α-tocopherol acetate (α-TOH-Ac) and an antioxidant compound, namely butylated hydroxytoluene (BHT) used to prevent TOHs autoxidation. The separation was carried out in a fused silica capillary of 100
μm I.D. and 375
μm O.D. packed in our laboratory with RP
18 silica stationary phase of either 5- or 3-μm diameter (23-cm long). The mobile phase was composed by mixtures of methanol (MeOH), acetonitrile (MeCN) and water. Typical analyses time for the separation of all the five components of the mixture were 6–9
min depending on the composition of the mobile phase. Efficiency and resolution were strongly influenced by the particle diameter and the highest
R
s and
N/
m values were observed using 3-μm RP
18 particles. Experiments performed with capillaries packed with 3-μm RP
18 particles provided good limit of detection (LOD) and limit of quantification (LOQ) (for δ-, γ-TOH, α-TOH-Ac were 4 and 8
μg/ml, while for α-TOH were 6 and 10
μg/ml, respectively). The optimized method was applied to extracts of serum and pharmaceutical preparation containing α-TOH and α-TOH-Ac.</description><subject>Analysis</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>Chromatography, Liquid - methods</subject><subject>Drugs</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General pharmacology</subject><subject>Medical sciences</subject><subject>Nano-LC</subject><subject>Nanotechnology - methods</subject><subject>Pharmacology. Drug treatments</subject><subject>Serum</subject><subject>Tocopherols</subject><subject>Tocopherols - chemistry</subject><subject>Tocopherols - isolation & purification</subject><subject>Vitamin E</subject><issn>0731-7085</issn><issn>1873-264X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0M9P2zAUwHFrYhpdtz9hKBfQdgh7rn8lJ4SqbSAh7VAm7WY5zzY1SuNgJ0j97wltBdw42YfPs5--hHyjcE6Byp8rUIyWCirxHdgPAMllCR_IjFaKlQvJ_x-R2Qs5Jp9zvgcAQWv-iRxTAZJxKWdErVxvkhlC7IroiyFi7NcuxTYXzbboTBfLNjyMwRa4TnFjhniXTL_efiEfvWmz-3o45-Tf71-3y6vy5u-f6-XlTYmspkPpG6yMUE4J6yk6L5CCNIDWKr8AYRqJlHPvaUMXlls0NTacT9v5SvnaWzYnZ_t3-xQfRpcHvQkZXduazsUxa0UrqChjExR7iCnmnJzXfQobk7aagn4upnfF9HMODUzvik2XOTk5fDA2G2dfpw6JJnB6ACajaX0yHYb8xsmFEHU1uYu9c1OOx-CSzhhch86G5HDQNoZ3VnkCmWmI7g</recordid><startdate>20040416</startdate><enddate>20040416</enddate><creator>Fanali, Salvatore</creator><creator>Camera, Emanuela</creator><creator>Chankvetadze, Bezhan</creator><creator>D’Orazio, Giovanni</creator><creator>Quaglia, Maria Giovanna</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20040416</creationdate><title>Separation of tocopherols by nano-liquid chromatography</title><author>Fanali, Salvatore ; Camera, Emanuela ; Chankvetadze, Bezhan ; D’Orazio, Giovanni ; Quaglia, Maria Giovanna</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c391t-fbc8a57e75df1cef5c106a0cdd7f205ab6c144ff1b12d4dca9cb44506f87f9fd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Analysis</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biological and medical sciences</topic><topic>Chromatography, Liquid - methods</topic><topic>Drugs</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General pharmacology</topic><topic>Medical sciences</topic><topic>Nano-LC</topic><topic>Nanotechnology - methods</topic><topic>Pharmacology. Drug treatments</topic><topic>Serum</topic><topic>Tocopherols</topic><topic>Tocopherols - chemistry</topic><topic>Tocopherols - isolation & purification</topic><topic>Vitamin E</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fanali, Salvatore</creatorcontrib><creatorcontrib>Camera, Emanuela</creatorcontrib><creatorcontrib>Chankvetadze, Bezhan</creatorcontrib><creatorcontrib>D’Orazio, Giovanni</creatorcontrib><creatorcontrib>Quaglia, Maria Giovanna</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of pharmaceutical and biomedical analysis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fanali, Salvatore</au><au>Camera, Emanuela</au><au>Chankvetadze, Bezhan</au><au>D’Orazio, Giovanni</au><au>Quaglia, Maria Giovanna</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Separation of tocopherols by nano-liquid chromatography</atitle><jtitle>Journal of pharmaceutical and biomedical analysis</jtitle><addtitle>J Pharm Biomed Anal</addtitle><date>2004-04-16</date><risdate>2004</risdate><volume>35</volume><issue>2</issue><spage>331</spage><epage>337</epage><pages>331-337</pages><issn>0731-7085</issn><eissn>1873-264X</eissn><coden>JPBADA</coden><abstract>Nanoliquid chromatography (nano-LC) was used for the separation of tocopherols (δ-, γ-, α-TOH), α-tocopherol acetate (α-TOH-Ac) and an antioxidant compound, namely butylated hydroxytoluene (BHT) used to prevent TOHs autoxidation. The separation was carried out in a fused silica capillary of 100
μm I.D. and 375
μm O.D. packed in our laboratory with RP
18 silica stationary phase of either 5- or 3-μm diameter (23-cm long). The mobile phase was composed by mixtures of methanol (MeOH), acetonitrile (MeCN) and water. Typical analyses time for the separation of all the five components of the mixture were 6–9
min depending on the composition of the mobile phase. Efficiency and resolution were strongly influenced by the particle diameter and the highest
R
s and
N/
m values were observed using 3-μm RP
18 particles. Experiments performed with capillaries packed with 3-μm RP
18 particles provided good limit of detection (LOD) and limit of quantification (LOQ) (for δ-, γ-TOH, α-TOH-Ac were 4 and 8
μg/ml, while for α-TOH were 6 and 10
μg/ml, respectively). The optimized method was applied to extracts of serum and pharmaceutical preparation containing α-TOH and α-TOH-Ac.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>15063466</pmid><doi>10.1016/S0731-7085(03)00646-0</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0731-7085 |
| ispartof | Journal of pharmaceutical and biomedical analysis, 2004-04, Vol.35 (2), p.331-337 |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Analysis Analytical, structural and metabolic biochemistry Biological and medical sciences Chromatography, Liquid - methods Drugs Fundamental and applied biological sciences. Psychology General pharmacology Medical sciences Nano-LC Nanotechnology - methods Pharmacology. Drug treatments Serum Tocopherols Tocopherols - chemistry Tocopherols - isolation & purification Vitamin E |
| title | Separation of tocopherols by nano-liquid chromatography |
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