Centrosome Maturation and Duplication in C. elegans Require the Coiled-Coil Protein SPD-2
Centrosomes are major determinants of mitotic spindle structure, but the mechanisms regulating their behavior remain poorly understood. The spd-2 gene of C. elegans is required for centrosome assembly or “maturation.” Here we show that spd-2 encodes a coiled-coil protein that localizes within perice...
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| Veröffentlicht in: | Developmental cell 2004-04, Vol.6 (4), p.511-523 |
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| creator | Kemp, Catherine A Kopish, Kevin R Zipperlen, Peder Ahringer, Julie O'Connell, Kevin F |
| description | Centrosomes are major determinants of mitotic spindle structure, but the mechanisms regulating their behavior remain poorly understood. The
spd-2 gene of
C. elegans is required for centrosome assembly or “maturation.” Here we show that
spd-2 encodes a coiled-coil protein that localizes within pericentriolar material (PCM) and in the immediate vicinity of centrioles. During maturation, SPD-2 gradually accumulates at the centrosome in a manner that is partially dependent on Aurora-A kinase and cytoplasmic dynein. Interestingly, SPD-2 interacts genetically with dynein heavy chain and SPD-5, another coiled-coil protein required for centrosome maturation. SPD-2 and SPD-5 are codependent for localization to the PCM, but SPD-2 localizes to centrioles independently of SPD-5. Surprisingly, we also find that SPD-2 is required for centrosome duplication and genetically interacts with ZYG-1, a kinase required for duplication. Thus, we have identified SPD-2 as a factor critical for the two basic functions of the centrosome—microtubule organization and duplication. |
| doi_str_mv | 10.1016/S1534-5807(04)00066-8 |
| format | Article |
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spd-2 gene of
C. elegans is required for centrosome assembly or “maturation.” Here we show that
spd-2 encodes a coiled-coil protein that localizes within pericentriolar material (PCM) and in the immediate vicinity of centrioles. During maturation, SPD-2 gradually accumulates at the centrosome in a manner that is partially dependent on Aurora-A kinase and cytoplasmic dynein. Interestingly, SPD-2 interacts genetically with dynein heavy chain and SPD-5, another coiled-coil protein required for centrosome maturation. SPD-2 and SPD-5 are codependent for localization to the PCM, but SPD-2 localizes to centrioles independently of SPD-5. Surprisingly, we also find that SPD-2 is required for centrosome duplication and genetically interacts with ZYG-1, a kinase required for duplication. Thus, we have identified SPD-2 as a factor critical for the two basic functions of the centrosome—microtubule organization and duplication.</description><identifier>ISSN: 1534-5807</identifier><identifier>EISSN: 1878-1551</identifier><identifier>DOI: 10.1016/S1534-5807(04)00066-8</identifier><identifier>PMID: 15068791</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Amino Acid Sequence - genetics ; Animals ; Aurora Kinases ; Base Sequence - genetics ; Caenorhabditis elegans ; Caenorhabditis elegans - genetics ; Caenorhabditis elegans - metabolism ; Caenorhabditis elegans Proteins - genetics ; Caenorhabditis elegans Proteins - isolation & purification ; Caenorhabditis elegans Proteins - metabolism ; Cell Cycle Proteins ; Cell Differentiation - genetics ; Centrioles - genetics ; Centrioles - metabolism ; Centrosome - metabolism ; DNA, Complementary - analysis ; DNA, Complementary - genetics ; Dyneins - genetics ; Dyneins - metabolism ; Microtubule-Organizing Center - metabolism ; Microtubules - genetics ; Microtubules - metabolism ; Mitosis - genetics ; Molecular Sequence Data ; Protein Kinases - genetics ; Protein Kinases - metabolism ; Protein Structure, Tertiary - genetics ; Protein-Serine-Threonine Kinases ; Sequence Homology, Amino Acid ; Spindle Apparatus - genetics ; Spindle Apparatus - metabolism ; Xenopus Proteins</subject><ispartof>Developmental cell, 2004-04, Vol.6 (4), p.511-523</ispartof><rights>2004 Cell Press</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c557t-d2bc8433b2fa7103c9fb6297903c238db33bfb1dd23836d2bbc4b7ff43d255f63</citedby><cites>FETCH-LOGICAL-c557t-d2bc8433b2fa7103c9fb6297903c238db33bfb1dd23836d2bbc4b7ff43d255f63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S1534-5807(04)00066-8$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3548,27922,27923,45993</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15068791$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kemp, Catherine A</creatorcontrib><creatorcontrib>Kopish, Kevin R</creatorcontrib><creatorcontrib>Zipperlen, Peder</creatorcontrib><creatorcontrib>Ahringer, Julie</creatorcontrib><creatorcontrib>O'Connell, Kevin F</creatorcontrib><title>Centrosome Maturation and Duplication in C. elegans Require the Coiled-Coil Protein SPD-2</title><title>Developmental cell</title><addtitle>Dev Cell</addtitle><description>Centrosomes are major determinants of mitotic spindle structure, but the mechanisms regulating their behavior remain poorly understood. The
spd-2 gene of
C. elegans is required for centrosome assembly or “maturation.” Here we show that
spd-2 encodes a coiled-coil protein that localizes within pericentriolar material (PCM) and in the immediate vicinity of centrioles. During maturation, SPD-2 gradually accumulates at the centrosome in a manner that is partially dependent on Aurora-A kinase and cytoplasmic dynein. Interestingly, SPD-2 interacts genetically with dynein heavy chain and SPD-5, another coiled-coil protein required for centrosome maturation. SPD-2 and SPD-5 are codependent for localization to the PCM, but SPD-2 localizes to centrioles independently of SPD-5. Surprisingly, we also find that SPD-2 is required for centrosome duplication and genetically interacts with ZYG-1, a kinase required for duplication. Thus, we have identified SPD-2 as a factor critical for the two basic functions of the centrosome—microtubule organization and duplication.</description><subject>Amino Acid Sequence - genetics</subject><subject>Animals</subject><subject>Aurora Kinases</subject><subject>Base Sequence - genetics</subject><subject>Caenorhabditis elegans</subject><subject>Caenorhabditis elegans - genetics</subject><subject>Caenorhabditis elegans - metabolism</subject><subject>Caenorhabditis elegans Proteins - genetics</subject><subject>Caenorhabditis elegans Proteins - isolation & purification</subject><subject>Caenorhabditis elegans Proteins - metabolism</subject><subject>Cell Cycle Proteins</subject><subject>Cell Differentiation - genetics</subject><subject>Centrioles - genetics</subject><subject>Centrioles - metabolism</subject><subject>Centrosome - metabolism</subject><subject>DNA, Complementary - analysis</subject><subject>DNA, Complementary - genetics</subject><subject>Dyneins - genetics</subject><subject>Dyneins - metabolism</subject><subject>Microtubule-Organizing Center - metabolism</subject><subject>Microtubules - genetics</subject><subject>Microtubules - metabolism</subject><subject>Mitosis - genetics</subject><subject>Molecular Sequence Data</subject><subject>Protein Kinases - genetics</subject><subject>Protein Kinases - metabolism</subject><subject>Protein Structure, Tertiary - genetics</subject><subject>Protein-Serine-Threonine Kinases</subject><subject>Sequence Homology, Amino Acid</subject><subject>Spindle Apparatus - genetics</subject><subject>Spindle Apparatus - metabolism</subject><subject>Xenopus Proteins</subject><issn>1534-5807</issn><issn>1878-1551</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkD1PwzAQhi0EoqXwE0CeEAwpdhLH9oRQ-JRAIAoDk5XYFzBKk9ZOkPj3uKSIsdPdyc_r0z0IHVIypYRmZzPKkjRigvATkp4SQrIsEltoTAUXEWWMbof-DxmhPe8_SchRQXbRiDKSCS7pGL3l0HSu9e0c8EPR9a7obNvgojH4sl_UVg-zbXA-xVDDe9F4_AzL3jrA3QfgvLU1mGhV8JNrOwjo7OkyivfRTlXUHg7WdYJer69e8tvo_vHmLr-4jzRjvItMXGqRJkkZVwWnJNGyKrNYchnaOBGmDE9VSY0JQ5IFutRpyasqTUzMWJUlE3Q8_Ltw7bIH36m59Rrqumig7b3i4WQu03gjSLmMpZQ0gGwAdRDjHVRq4ey8cN-KErWSr37lq5VZRVL1K1-JkDtaL-jLOZj_1Np2AM4HAIKPLwtOeW2h0WCCTd0p09oNK34AzS-S8w</recordid><startdate>20040401</startdate><enddate>20040401</enddate><creator>Kemp, Catherine A</creator><creator>Kopish, Kevin R</creator><creator>Zipperlen, Peder</creator><creator>Ahringer, Julie</creator><creator>O'Connell, Kevin F</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20040401</creationdate><title>Centrosome Maturation and Duplication in C. elegans Require the Coiled-Coil Protein SPD-2</title><author>Kemp, Catherine A ; Kopish, Kevin R ; Zipperlen, Peder ; Ahringer, Julie ; O'Connell, Kevin F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c557t-d2bc8433b2fa7103c9fb6297903c238db33bfb1dd23836d2bbc4b7ff43d255f63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Amino Acid Sequence - genetics</topic><topic>Animals</topic><topic>Aurora Kinases</topic><topic>Base Sequence - genetics</topic><topic>Caenorhabditis elegans</topic><topic>Caenorhabditis elegans - genetics</topic><topic>Caenorhabditis elegans - metabolism</topic><topic>Caenorhabditis elegans Proteins - genetics</topic><topic>Caenorhabditis elegans Proteins - isolation & purification</topic><topic>Caenorhabditis elegans Proteins - metabolism</topic><topic>Cell Cycle Proteins</topic><topic>Cell Differentiation - genetics</topic><topic>Centrioles - genetics</topic><topic>Centrioles - metabolism</topic><topic>Centrosome - metabolism</topic><topic>DNA, Complementary - analysis</topic><topic>DNA, Complementary - genetics</topic><topic>Dyneins - genetics</topic><topic>Dyneins - metabolism</topic><topic>Microtubule-Organizing Center - metabolism</topic><topic>Microtubules - genetics</topic><topic>Microtubules - metabolism</topic><topic>Mitosis - genetics</topic><topic>Molecular Sequence Data</topic><topic>Protein Kinases - genetics</topic><topic>Protein Kinases - metabolism</topic><topic>Protein Structure, Tertiary - genetics</topic><topic>Protein-Serine-Threonine Kinases</topic><topic>Sequence Homology, Amino Acid</topic><topic>Spindle Apparatus - genetics</topic><topic>Spindle Apparatus - metabolism</topic><topic>Xenopus Proteins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kemp, Catherine A</creatorcontrib><creatorcontrib>Kopish, Kevin R</creatorcontrib><creatorcontrib>Zipperlen, Peder</creatorcontrib><creatorcontrib>Ahringer, Julie</creatorcontrib><creatorcontrib>O'Connell, Kevin F</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Developmental cell</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kemp, Catherine A</au><au>Kopish, Kevin R</au><au>Zipperlen, Peder</au><au>Ahringer, Julie</au><au>O'Connell, Kevin F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Centrosome Maturation and Duplication in C. elegans Require the Coiled-Coil Protein SPD-2</atitle><jtitle>Developmental cell</jtitle><addtitle>Dev Cell</addtitle><date>2004-04-01</date><risdate>2004</risdate><volume>6</volume><issue>4</issue><spage>511</spage><epage>523</epage><pages>511-523</pages><issn>1534-5807</issn><eissn>1878-1551</eissn><abstract>Centrosomes are major determinants of mitotic spindle structure, but the mechanisms regulating their behavior remain poorly understood. The
spd-2 gene of
C. elegans is required for centrosome assembly or “maturation.” Here we show that
spd-2 encodes a coiled-coil protein that localizes within pericentriolar material (PCM) and in the immediate vicinity of centrioles. During maturation, SPD-2 gradually accumulates at the centrosome in a manner that is partially dependent on Aurora-A kinase and cytoplasmic dynein. Interestingly, SPD-2 interacts genetically with dynein heavy chain and SPD-5, another coiled-coil protein required for centrosome maturation. SPD-2 and SPD-5 are codependent for localization to the PCM, but SPD-2 localizes to centrioles independently of SPD-5. Surprisingly, we also find that SPD-2 is required for centrosome duplication and genetically interacts with ZYG-1, a kinase required for duplication. Thus, we have identified SPD-2 as a factor critical for the two basic functions of the centrosome—microtubule organization and duplication.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>15068791</pmid><doi>10.1016/S1534-5807(04)00066-8</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Amino Acid Sequence - genetics Animals Aurora Kinases Base Sequence - genetics Caenorhabditis elegans Caenorhabditis elegans - genetics Caenorhabditis elegans - metabolism Caenorhabditis elegans Proteins - genetics Caenorhabditis elegans Proteins - isolation & purification Caenorhabditis elegans Proteins - metabolism Cell Cycle Proteins Cell Differentiation - genetics Centrioles - genetics Centrioles - metabolism Centrosome - metabolism DNA, Complementary - analysis DNA, Complementary - genetics Dyneins - genetics Dyneins - metabolism Microtubule-Organizing Center - metabolism Microtubules - genetics Microtubules - metabolism Mitosis - genetics Molecular Sequence Data Protein Kinases - genetics Protein Kinases - metabolism Protein Structure, Tertiary - genetics Protein-Serine-Threonine Kinases Sequence Homology, Amino Acid Spindle Apparatus - genetics Spindle Apparatus - metabolism Xenopus Proteins |
| title | Centrosome Maturation and Duplication in C. elegans Require the Coiled-Coil Protein SPD-2 |
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