Molecular epidemiology of cutaneous leishmaniasis in Venezuela
This paper discusses the utility of a set of primers (3J1, 3J2) designed from a repetitive nuclear deoxyribonucleic acid sequence for the diagnosis of Leishmania braziliensis infection in samples obtained from humans, insect vectors and mammalian reservoir hosts from different endemic areas in Venez...
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| Veröffentlicht in: | Transactions of the Royal Society of Tropical Medicine and Hygiene 2002-04, Vol.96 (Supplement-1), p.S105-S109 |
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| container_issue | Supplement-1 |
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| container_title | Transactions of the Royal Society of Tropical Medicine and Hygiene |
| container_volume | 96 |
| creator | Rodriguez, Noris De Lima, Hector Aguilar, Cruz M. Rodriguez, Armando Barker, Douglas C. Convit, Jacinto |
| description | This paper discusses the utility of a set of primers (3J1, 3J2) designed from a repetitive nuclear deoxyribonucleic acid sequence for the diagnosis of
Leishmania braziliensis infection in samples obtained from humans, insect vectors and mammalian reservoir hosts from different endemic areas in Venezuela. A high incidence of
Leishmania (Viannia) braziliensis infection was found in the endemic areas studied. The sensitivity and specificity of the primers used were adequate for the identification of the natural vectors and reservoir hosts of
L. (V.) braziliensis. The polymerase chain reaction was more sensitive than culture and stained smear examination in the diagnosis of cutaneous leishmaniasis, detecting 80% of cases compared to 42% and 72%, respectively. |
| doi_str_mv | 10.1016/S0035-9203(02)90060-X |
| format | Article |
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Leishmania braziliensis infection in samples obtained from humans, insect vectors and mammalian reservoir hosts from different endemic areas in Venezuela. A high incidence of
Leishmania (Viannia) braziliensis infection was found in the endemic areas studied. The sensitivity and specificity of the primers used were adequate for the identification of the natural vectors and reservoir hosts of
L. (V.) braziliensis. The polymerase chain reaction was more sensitive than culture and stained smear examination in the diagnosis of cutaneous leishmaniasis, detecting 80% of cases compared to 42% and 72%, respectively.</description><identifier>ISSN: 0035-9203</identifier><identifier>EISSN: 1878-3503</identifier><identifier>DOI: 10.1016/S0035-9203(02)90060-X</identifier><identifier>PMID: 12055822</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Animals ; diagnosis ; DNA Primers ; DNA, Protozoan - analysis ; Endemic Diseases ; epidemiology ; Humans ; Insect Vectors - parasitology ; Leishmania braziliensis ; Leishmania braziliensis - classification ; Leishmania braziliensis - genetics ; leishmaniasis ; Leishmaniasis, Cutaneous - diagnosis ; Leishmaniasis, Cutaneous - epidemiology ; Leishmaniasis, Cutaneous - veterinary ; Lutzomyia gomezi ; Lutzomyia panamensis ; Mammals - parasitology ; Polymerase Chain Reaction - methods ; Psychodidae - parasitology ; Rattus rattus ; Sensitivity and Specificity ; Venezuela ; Venezuela - epidemiology</subject><ispartof>Transactions of the Royal Society of Tropical Medicine and Hygiene, 2002-04, Vol.96 (Supplement-1), p.S105-S109</ispartof><rights>2002</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c316t-8210ccce856f24bd30362d99a45a3a1b059faaf3084772a4e6ca3e08d9b63e383</citedby><cites>FETCH-LOGICAL-c316t-8210ccce856f24bd30362d99a45a3a1b059faaf3084772a4e6ca3e08d9b63e383</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12055822$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rodriguez, Noris</creatorcontrib><creatorcontrib>De Lima, Hector</creatorcontrib><creatorcontrib>Aguilar, Cruz M.</creatorcontrib><creatorcontrib>Rodriguez, Armando</creatorcontrib><creatorcontrib>Barker, Douglas C.</creatorcontrib><creatorcontrib>Convit, Jacinto</creatorcontrib><title>Molecular epidemiology of cutaneous leishmaniasis in Venezuela</title><title>Transactions of the Royal Society of Tropical Medicine and Hygiene</title><addtitle>Trans R Soc Trop Med Hyg</addtitle><description>This paper discusses the utility of a set of primers (3J1, 3J2) designed from a repetitive nuclear deoxyribonucleic acid sequence for the diagnosis of
Leishmania braziliensis infection in samples obtained from humans, insect vectors and mammalian reservoir hosts from different endemic areas in Venezuela. A high incidence of
Leishmania (Viannia) braziliensis infection was found in the endemic areas studied. The sensitivity and specificity of the primers used were adequate for the identification of the natural vectors and reservoir hosts of
L. (V.) braziliensis. The polymerase chain reaction was more sensitive than culture and stained smear examination in the diagnosis of cutaneous leishmaniasis, detecting 80% of cases compared to 42% and 72%, respectively.</description><subject>Animals</subject><subject>diagnosis</subject><subject>DNA Primers</subject><subject>DNA, Protozoan - analysis</subject><subject>Endemic Diseases</subject><subject>epidemiology</subject><subject>Humans</subject><subject>Insect Vectors - parasitology</subject><subject>Leishmania braziliensis</subject><subject>Leishmania braziliensis - classification</subject><subject>Leishmania braziliensis - genetics</subject><subject>leishmaniasis</subject><subject>Leishmaniasis, Cutaneous - diagnosis</subject><subject>Leishmaniasis, Cutaneous - epidemiology</subject><subject>Leishmaniasis, Cutaneous - veterinary</subject><subject>Lutzomyia gomezi</subject><subject>Lutzomyia panamensis</subject><subject>Mammals - parasitology</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Psychodidae - parasitology</subject><subject>Rattus rattus</subject><subject>Sensitivity and Specificity</subject><subject>Venezuela</subject><subject>Venezuela - epidemiology</subject><issn>0035-9203</issn><issn>1878-3503</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1P3DAQhq0KVJZtfwJVTggOgbEdO84FVK3oLtIikNhWKy6W40xal3xs7aRi--sbyAqOnOYwzzvz6iHkiMIZBSrP7wG4iDMG_ATYaQYgIV5_IBOqUhVzAXyPTF6RA3IYwm8AJqjIPpIDykAIxdiEXNy0Fdq-Mj7CjSuwdm3V_txGbRnZvjMNtn2IKnThV20aZ4ILkWuiH9jgvx4r84nsl6YK-Hk3p-T7t6vVbBEvb-fXs6_L2HIqu1gxCtZaVEKWLMkLDlyyIstMIgw3NAeRlcaUHFSSpswkKK3hCKrIcsmRKz4lx-PdjW__9Bg6XbtgsarGhjqlCpJEsgEUI2h9G4LHUm-8q43fagr6WZx-EaefrWhg-kWcXg-5L7sHfV5j8ZbamRqAeARc6PDpdW_8o5YpT4VerB_0an6v6GJ1p7OBvxx5HLT8deh1sA4bi4XzaDtdtO6dSv8B2IaMpA</recordid><startdate>200204</startdate><enddate>200204</enddate><creator>Rodriguez, Noris</creator><creator>De Lima, Hector</creator><creator>Aguilar, Cruz M.</creator><creator>Rodriguez, Armando</creator><creator>Barker, Douglas C.</creator><creator>Convit, Jacinto</creator><general>Elsevier Ltd</general><general>Royal Society of Tropical Medicine and Hygiene</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200204</creationdate><title>Molecular epidemiology of cutaneous leishmaniasis in Venezuela</title><author>Rodriguez, Noris ; De Lima, Hector ; Aguilar, Cruz M. ; Rodriguez, Armando ; Barker, Douglas C. ; Convit, Jacinto</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c316t-8210ccce856f24bd30362d99a45a3a1b059faaf3084772a4e6ca3e08d9b63e383</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Animals</topic><topic>diagnosis</topic><topic>DNA Primers</topic><topic>DNA, Protozoan - analysis</topic><topic>Endemic Diseases</topic><topic>epidemiology</topic><topic>Humans</topic><topic>Insect Vectors - parasitology</topic><topic>Leishmania braziliensis</topic><topic>Leishmania braziliensis - classification</topic><topic>Leishmania braziliensis - genetics</topic><topic>leishmaniasis</topic><topic>Leishmaniasis, Cutaneous - diagnosis</topic><topic>Leishmaniasis, Cutaneous - epidemiology</topic><topic>Leishmaniasis, Cutaneous - veterinary</topic><topic>Lutzomyia gomezi</topic><topic>Lutzomyia panamensis</topic><topic>Mammals - parasitology</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Psychodidae - parasitology</topic><topic>Rattus rattus</topic><topic>Sensitivity and Specificity</topic><topic>Venezuela</topic><topic>Venezuela - epidemiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rodriguez, Noris</creatorcontrib><creatorcontrib>De Lima, Hector</creatorcontrib><creatorcontrib>Aguilar, Cruz M.</creatorcontrib><creatorcontrib>Rodriguez, Armando</creatorcontrib><creatorcontrib>Barker, Douglas C.</creatorcontrib><creatorcontrib>Convit, Jacinto</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Transactions of the Royal Society of Tropical Medicine and Hygiene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rodriguez, Noris</au><au>De Lima, Hector</au><au>Aguilar, Cruz M.</au><au>Rodriguez, Armando</au><au>Barker, Douglas C.</au><au>Convit, Jacinto</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular epidemiology of cutaneous leishmaniasis in Venezuela</atitle><jtitle>Transactions of the Royal Society of Tropical Medicine and Hygiene</jtitle><addtitle>Trans R Soc Trop Med Hyg</addtitle><date>2002-04</date><risdate>2002</risdate><volume>96</volume><issue>Supplement-1</issue><spage>S105</spage><epage>S109</epage><pages>S105-S109</pages><issn>0035-9203</issn><eissn>1878-3503</eissn><abstract>This paper discusses the utility of a set of primers (3J1, 3J2) designed from a repetitive nuclear deoxyribonucleic acid sequence for the diagnosis of
Leishmania braziliensis infection in samples obtained from humans, insect vectors and mammalian reservoir hosts from different endemic areas in Venezuela. A high incidence of
Leishmania (Viannia) braziliensis infection was found in the endemic areas studied. The sensitivity and specificity of the primers used were adequate for the identification of the natural vectors and reservoir hosts of
L. (V.) braziliensis. The polymerase chain reaction was more sensitive than culture and stained smear examination in the diagnosis of cutaneous leishmaniasis, detecting 80% of cases compared to 42% and 72%, respectively.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>12055822</pmid><doi>10.1016/S0035-9203(02)90060-X</doi></addata></record> |
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| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current); Alma/SFX Local Collection |
| subjects | Animals diagnosis DNA Primers DNA, Protozoan - analysis Endemic Diseases epidemiology Humans Insect Vectors - parasitology Leishmania braziliensis Leishmania braziliensis - classification Leishmania braziliensis - genetics leishmaniasis Leishmaniasis, Cutaneous - diagnosis Leishmaniasis, Cutaneous - epidemiology Leishmaniasis, Cutaneous - veterinary Lutzomyia gomezi Lutzomyia panamensis Mammals - parasitology Polymerase Chain Reaction - methods Psychodidae - parasitology Rattus rattus Sensitivity and Specificity Venezuela Venezuela - epidemiology |
| title | Molecular epidemiology of cutaneous leishmaniasis in Venezuela |
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