Mediator Factor Med8p Interacts with the Hexokinase 2: Implication in the Glucose Signalling Pathway of Saccharomyces cerevisiae
In the presence of glucose the protein hexokinase 2 (Hxk2p), normally resident in the cytosol, is translocated to the nucleus where it impairs the activation of transcription of the glucose-repressed genes HXK1, GLK1 and SUC2, and promotes the activation of transcription of the glucose-induced genes...
Gespeichert in:
| Veröffentlicht in: | Journal of molecular biology 2002-06, Vol.319 (3), p.703-714 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 714 |
|---|---|
| container_issue | 3 |
| container_start_page | 703 |
| container_title | Journal of molecular biology |
| container_volume | 319 |
| creator | de la Cera, T Herrero, P Moreno-Herrero, F Chaves, R.S Moreno, F |
| description | In the presence of glucose the protein hexokinase 2 (Hxk2p), normally resident in the cytosol, is translocated to the nucleus where it impairs the activation of transcription of the glucose-repressed genes
HXK1,
GLK1 and
SUC2, and promotes the activation of transcription of the glucose-induced genes
HXK2 and
HXT1. Here, we demonstrate the involvement of an heptameric motif, named the MED8 site, in the direct binding of the mediator protein Med8p, either as a monomer or as a homodimer. Because this site was previously involved in the Hxk2p-dependent glucose-induced regulation of gene transcription, we tested whether Hxk2p interacts with Med8p. Our results show that Hxk2 and Med8 proteins are physically associated and that this Hxk2p–Med8p interaction is of physiological significance because both proteins have been found interacting together in a cluster with DNA fragments containing the MED8 site. We conclude that Hxk2p operates through the MED8 site, by interacting with Med8p, in the glucose signal transduction pathway of
Saccharomyces cerevisiae. |
| doi_str_mv | 10.1016/S0022-2836(02)00377-7 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_71804398</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0022283602003777</els_id><sourcerecordid>71804398</sourcerecordid><originalsourceid>FETCH-LOGICAL-c392t-78f34dc65748daa2ec25795f9775c7ed2e32bfc5d9f51a35fd6b3245a5581ad33</originalsourceid><addsrcrecordid>eNqFkUtvFDEMgCMEokvhJ4ByQvQwkMdkkuGCUNXHSkUgLZwjb-LpBmYm2yTbsjd-OrMPwbEny_ZnW_JHyGvO3nPGmw8LxoSohJHNOybOGJNaV_oJmXFm2so00jwls3_ICXmR80_GmJK1eU5OuGCqNk09I3--oA9QYqKX4HZhys2azseCaSpk-hDKipYV0mv8HX-FETJS8ZHOh3UfHJQQRxrGPXDVb1ycuotwO0Lfh_GWfoOyeoAtjR1dgHMrSHHYOszUYcL7kAPgS_Ksgz7jq2M8JT8uL76fX1c3X6_m559vKidbUSptOll71yhdGw8g0AmlW9W1Wiun0QuUYtk55dtOcZCq881SilqBUoaDl_KUvD3sXad4t8Fc7BCyw76HEeMmW80Nq2VrHgW5qVnbCj2B6gC6FHNO2Nl1CgOkreXM7hzZvSO7E2CZsHtHdjf35nhgsxzQ_586SpmATwcAp3_cB0w2u4Cjm0wldMX6GB458RdAVaIY</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>18409927</pqid></control><display><type>article</type><title>Mediator Factor Med8p Interacts with the Hexokinase 2: Implication in the Glucose Signalling Pathway of Saccharomyces cerevisiae</title><source>MEDLINE</source><source>ScienceDirect Journals (5 years ago - present)</source><creator>de la Cera, T ; Herrero, P ; Moreno-Herrero, F ; Chaves, R.S ; Moreno, F</creator><creatorcontrib>de la Cera, T ; Herrero, P ; Moreno-Herrero, F ; Chaves, R.S ; Moreno, F</creatorcontrib><description>In the presence of glucose the protein hexokinase 2 (Hxk2p), normally resident in the cytosol, is translocated to the nucleus where it impairs the activation of transcription of the glucose-repressed genes
HXK1,
GLK1 and
SUC2, and promotes the activation of transcription of the glucose-induced genes
HXK2 and
HXT1. Here, we demonstrate the involvement of an heptameric motif, named the MED8 site, in the direct binding of the mediator protein Med8p, either as a monomer or as a homodimer. Because this site was previously involved in the Hxk2p-dependent glucose-induced regulation of gene transcription, we tested whether Hxk2p interacts with Med8p. Our results show that Hxk2 and Med8 proteins are physically associated and that this Hxk2p–Med8p interaction is of physiological significance because both proteins have been found interacting together in a cluster with DNA fragments containing the MED8 site. We conclude that Hxk2p operates through the MED8 site, by interacting with Med8p, in the glucose signal transduction pathway of
Saccharomyces cerevisiae.</description><identifier>ISSN: 0022-2836</identifier><identifier>EISSN: 1089-8638</identifier><identifier>DOI: 10.1016/S0022-2836(02)00377-7</identifier><identifier>PMID: 12054864</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Dimerization ; DNA-Binding Proteins - chemistry ; DNA-Binding Proteins - metabolism ; Fungal Proteins - chemistry ; Fungal Proteins - metabolism ; Gene Expression Regulation, Fungal - drug effects ; Genes, Fungal - genetics ; Glucose - pharmacology ; glucose repression ; Hexokinase - chemistry ; Hexokinase - genetics ; Hexokinase - metabolism ; Hxk2p ; Med8p ; Mediator Complex ; Microscopy, Atomic Force ; Precipitin Tests ; Protein Binding ; protein interactions ; Protein Structure, Quaternary ; Protein Structure, Tertiary ; Saccharomyces cerevisiae ; Saccharomyces cerevisiae - drug effects ; Saccharomyces cerevisiae - enzymology ; Saccharomyces cerevisiae - genetics ; Saccharomyces cerevisiae - metabolism ; Saccharomyces cerevisiae Proteins ; Signal Transduction - drug effects ; Transcription Factors - chemistry ; Transcription Factors - genetics ; Transcription Factors - metabolism ; Two-Hybrid System Techniques</subject><ispartof>Journal of molecular biology, 2002-06, Vol.319 (3), p.703-714</ispartof><rights>2002 Elsevier Science Ltd</rights><rights>(c) 2002 Elsevier Science Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c392t-78f34dc65748daa2ec25795f9775c7ed2e32bfc5d9f51a35fd6b3245a5581ad33</citedby><cites>FETCH-LOGICAL-c392t-78f34dc65748daa2ec25795f9775c7ed2e32bfc5d9f51a35fd6b3245a5581ad33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0022-2836(02)00377-7$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12054864$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>de la Cera, T</creatorcontrib><creatorcontrib>Herrero, P</creatorcontrib><creatorcontrib>Moreno-Herrero, F</creatorcontrib><creatorcontrib>Chaves, R.S</creatorcontrib><creatorcontrib>Moreno, F</creatorcontrib><title>Mediator Factor Med8p Interacts with the Hexokinase 2: Implication in the Glucose Signalling Pathway of Saccharomyces cerevisiae</title><title>Journal of molecular biology</title><addtitle>J Mol Biol</addtitle><description>In the presence of glucose the protein hexokinase 2 (Hxk2p), normally resident in the cytosol, is translocated to the nucleus where it impairs the activation of transcription of the glucose-repressed genes
HXK1,
GLK1 and
SUC2, and promotes the activation of transcription of the glucose-induced genes
HXK2 and
HXT1. Here, we demonstrate the involvement of an heptameric motif, named the MED8 site, in the direct binding of the mediator protein Med8p, either as a monomer or as a homodimer. Because this site was previously involved in the Hxk2p-dependent glucose-induced regulation of gene transcription, we tested whether Hxk2p interacts with Med8p. Our results show that Hxk2 and Med8 proteins are physically associated and that this Hxk2p–Med8p interaction is of physiological significance because both proteins have been found interacting together in a cluster with DNA fragments containing the MED8 site. We conclude that Hxk2p operates through the MED8 site, by interacting with Med8p, in the glucose signal transduction pathway of
Saccharomyces cerevisiae.</description><subject>Dimerization</subject><subject>DNA-Binding Proteins - chemistry</subject><subject>DNA-Binding Proteins - metabolism</subject><subject>Fungal Proteins - chemistry</subject><subject>Fungal Proteins - metabolism</subject><subject>Gene Expression Regulation, Fungal - drug effects</subject><subject>Genes, Fungal - genetics</subject><subject>Glucose - pharmacology</subject><subject>glucose repression</subject><subject>Hexokinase - chemistry</subject><subject>Hexokinase - genetics</subject><subject>Hexokinase - metabolism</subject><subject>Hxk2p</subject><subject>Med8p</subject><subject>Mediator Complex</subject><subject>Microscopy, Atomic Force</subject><subject>Precipitin Tests</subject><subject>Protein Binding</subject><subject>protein interactions</subject><subject>Protein Structure, Quaternary</subject><subject>Protein Structure, Tertiary</subject><subject>Saccharomyces cerevisiae</subject><subject>Saccharomyces cerevisiae - drug effects</subject><subject>Saccharomyces cerevisiae - enzymology</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Saccharomyces cerevisiae - metabolism</subject><subject>Saccharomyces cerevisiae Proteins</subject><subject>Signal Transduction - drug effects</subject><subject>Transcription Factors - chemistry</subject><subject>Transcription Factors - genetics</subject><subject>Transcription Factors - metabolism</subject><subject>Two-Hybrid System Techniques</subject><issn>0022-2836</issn><issn>1089-8638</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtvFDEMgCMEokvhJ4ByQvQwkMdkkuGCUNXHSkUgLZwjb-LpBmYm2yTbsjd-OrMPwbEny_ZnW_JHyGvO3nPGmw8LxoSohJHNOybOGJNaV_oJmXFm2so00jwls3_ICXmR80_GmJK1eU5OuGCqNk09I3--oA9QYqKX4HZhys2azseCaSpk-hDKipYV0mv8HX-FETJS8ZHOh3UfHJQQRxrGPXDVb1ycuotwO0Lfh_GWfoOyeoAtjR1dgHMrSHHYOszUYcL7kAPgS_Ksgz7jq2M8JT8uL76fX1c3X6_m559vKidbUSptOll71yhdGw8g0AmlW9W1Wiun0QuUYtk55dtOcZCq881SilqBUoaDl_KUvD3sXad4t8Fc7BCyw76HEeMmW80Nq2VrHgW5qVnbCj2B6gC6FHNO2Nl1CgOkreXM7hzZvSO7E2CZsHtHdjf35nhgsxzQ_586SpmATwcAp3_cB0w2u4Cjm0wldMX6GB458RdAVaIY</recordid><startdate>20020607</startdate><enddate>20020607</enddate><creator>de la Cera, T</creator><creator>Herrero, P</creator><creator>Moreno-Herrero, F</creator><creator>Chaves, R.S</creator><creator>Moreno, F</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>20020607</creationdate><title>Mediator Factor Med8p Interacts with the Hexokinase 2: Implication in the Glucose Signalling Pathway of Saccharomyces cerevisiae</title><author>de la Cera, T ; Herrero, P ; Moreno-Herrero, F ; Chaves, R.S ; Moreno, F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c392t-78f34dc65748daa2ec25795f9775c7ed2e32bfc5d9f51a35fd6b3245a5581ad33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Dimerization</topic><topic>DNA-Binding Proteins - chemistry</topic><topic>DNA-Binding Proteins - metabolism</topic><topic>Fungal Proteins - chemistry</topic><topic>Fungal Proteins - metabolism</topic><topic>Gene Expression Regulation, Fungal - drug effects</topic><topic>Genes, Fungal - genetics</topic><topic>Glucose - pharmacology</topic><topic>glucose repression</topic><topic>Hexokinase - chemistry</topic><topic>Hexokinase - genetics</topic><topic>Hexokinase - metabolism</topic><topic>Hxk2p</topic><topic>Med8p</topic><topic>Mediator Complex</topic><topic>Microscopy, Atomic Force</topic><topic>Precipitin Tests</topic><topic>Protein Binding</topic><topic>protein interactions</topic><topic>Protein Structure, Quaternary</topic><topic>Protein Structure, Tertiary</topic><topic>Saccharomyces cerevisiae</topic><topic>Saccharomyces cerevisiae - drug effects</topic><topic>Saccharomyces cerevisiae - enzymology</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>Saccharomyces cerevisiae - metabolism</topic><topic>Saccharomyces cerevisiae Proteins</topic><topic>Signal Transduction - drug effects</topic><topic>Transcription Factors - chemistry</topic><topic>Transcription Factors - genetics</topic><topic>Transcription Factors - metabolism</topic><topic>Two-Hybrid System Techniques</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>de la Cera, T</creatorcontrib><creatorcontrib>Herrero, P</creatorcontrib><creatorcontrib>Moreno-Herrero, F</creatorcontrib><creatorcontrib>Chaves, R.S</creatorcontrib><creatorcontrib>Moreno, F</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>de la Cera, T</au><au>Herrero, P</au><au>Moreno-Herrero, F</au><au>Chaves, R.S</au><au>Moreno, F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mediator Factor Med8p Interacts with the Hexokinase 2: Implication in the Glucose Signalling Pathway of Saccharomyces cerevisiae</atitle><jtitle>Journal of molecular biology</jtitle><addtitle>J Mol Biol</addtitle><date>2002-06-07</date><risdate>2002</risdate><volume>319</volume><issue>3</issue><spage>703</spage><epage>714</epage><pages>703-714</pages><issn>0022-2836</issn><eissn>1089-8638</eissn><abstract>In the presence of glucose the protein hexokinase 2 (Hxk2p), normally resident in the cytosol, is translocated to the nucleus where it impairs the activation of transcription of the glucose-repressed genes
HXK1,
GLK1 and
SUC2, and promotes the activation of transcription of the glucose-induced genes
HXK2 and
HXT1. Here, we demonstrate the involvement of an heptameric motif, named the MED8 site, in the direct binding of the mediator protein Med8p, either as a monomer or as a homodimer. Because this site was previously involved in the Hxk2p-dependent glucose-induced regulation of gene transcription, we tested whether Hxk2p interacts with Med8p. Our results show that Hxk2 and Med8 proteins are physically associated and that this Hxk2p–Med8p interaction is of physiological significance because both proteins have been found interacting together in a cluster with DNA fragments containing the MED8 site. We conclude that Hxk2p operates through the MED8 site, by interacting with Med8p, in the glucose signal transduction pathway of
Saccharomyces cerevisiae.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>12054864</pmid><doi>10.1016/S0022-2836(02)00377-7</doi><tpages>12</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-2836 |
| ispartof | Journal of molecular biology, 2002-06, Vol.319 (3), p.703-714 |
| issn | 0022-2836 1089-8638 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_71804398 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Dimerization DNA-Binding Proteins - chemistry DNA-Binding Proteins - metabolism Fungal Proteins - chemistry Fungal Proteins - metabolism Gene Expression Regulation, Fungal - drug effects Genes, Fungal - genetics Glucose - pharmacology glucose repression Hexokinase - chemistry Hexokinase - genetics Hexokinase - metabolism Hxk2p Med8p Mediator Complex Microscopy, Atomic Force Precipitin Tests Protein Binding protein interactions Protein Structure, Quaternary Protein Structure, Tertiary Saccharomyces cerevisiae Saccharomyces cerevisiae - drug effects Saccharomyces cerevisiae - enzymology Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - metabolism Saccharomyces cerevisiae Proteins Signal Transduction - drug effects Transcription Factors - chemistry Transcription Factors - genetics Transcription Factors - metabolism Two-Hybrid System Techniques |
| title | Mediator Factor Med8p Interacts with the Hexokinase 2: Implication in the Glucose Signalling Pathway of Saccharomyces cerevisiae |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-12T06%3A26%3A51IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Mediator%20Factor%20Med8p%20Interacts%20with%20the%20Hexokinase%202:%20Implication%20in%20the%20Glucose%20Signalling%20Pathway%20of%20Saccharomyces%20cerevisiae&rft.jtitle=Journal%20of%20molecular%20biology&rft.au=de%20la%20Cera,%20T&rft.date=2002-06-07&rft.volume=319&rft.issue=3&rft.spage=703&rft.epage=714&rft.pages=703-714&rft.issn=0022-2836&rft.eissn=1089-8638&rft_id=info:doi/10.1016/S0022-2836(02)00377-7&rft_dat=%3Cproquest_cross%3E71804398%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=18409927&rft_id=info:pmid/12054864&rft_els_id=S0022283602003777&rfr_iscdi=true |