The Influence of Lactobacillus brevis on Ornithine Decarboxylase Activity and Polyamine Profiles in Helicobacter pylori-Infected Gastric Mucosa
ABSTRACT Background. Functional probiotics may prevent Helicobacter pylori infection, and some evidence suggests that they also possess antitumor properties. Lactobacillus brevis (CD2) is a functional Lactobacillus strain with peculiar biochemical features, essentially related to the activity of ar...
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| Veröffentlicht in: | Helicobacter (Cambridge, Mass.) Mass.), 2004-04, Vol.9 (2), p.165-172 |
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| creator | Linsalata, Michele Russo, Francesco Berloco, Pasquale Caruso, Maria Lucia Matteo, Giovanni D.I. Cifone, Maria Grazia Simone, Claudio D.E. Ierardi, Enzo Leo, Alfredo Di |
| description | ABSTRACT
Background. Functional probiotics may prevent Helicobacter pylori infection, and some evidence suggests that they also possess antitumor properties. Lactobacillus brevis (CD2) is a functional Lactobacillus strain with peculiar biochemical features, essentially related to the activity of arginine deiminase. This enzyme catalyzes the catabolism of arginine and affects the biosynthesis of polyamines (putrescine, spermidine, and spermine). Polyamines are polycations found in high concentrations in both normal and neoplastic cells. Our aims were: 1, to assess whether oral administration of L. brevis (CD2) affects H. pylori survival in the human gastric mucosa; 2, to evaluate the effects of L. brevis (CD2) on polyamine biosynthesis in gastric biopsies from H. pylori‐positive patients.
Materials and Methods. For 3 weeks before endoscopy, 22 H. pylori‐positive dyspeptic patients randomly received (ratio 1 : 1) high oral doses of L. brevis (CD2) or placebo. Before and after treatment, H. pylori infection was determined by urea breath test (UBT). In gastric biopsies, ornithine decarboxylase activity and polyamine levels were, respectively, evaluated by a radiometric technique and high‐pressure liquid chromatography (HPLC).
Results. L. brevis (CD2) treatment did not eradicate H. pylori. However, a reduction in the UBT delta values occurred, suggesting a decrease in intragastric bacterial load. Significantly, L. brevis (CD2) induced a decrease in gastric ornithine decarboxylase activity and polyamine levels.
Conclusions. Our data support the hypothesis that L. brevis (CD2) treatment decreases H. pylori colonization, thus reducing polyamine biosynthesis. Alternatively, the arginine deiminase activity following L. brevis (CD2) administration might cause arginine deficiency, preventing polyamine generation from gastric cells. |
| doi_str_mv | 10.1111/j.1083-4389.2004.00214.x |
| format | Article |
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Background. Functional probiotics may prevent Helicobacter pylori infection, and some evidence suggests that they also possess antitumor properties. Lactobacillus brevis (CD2) is a functional Lactobacillus strain with peculiar biochemical features, essentially related to the activity of arginine deiminase. This enzyme catalyzes the catabolism of arginine and affects the biosynthesis of polyamines (putrescine, spermidine, and spermine). Polyamines are polycations found in high concentrations in both normal and neoplastic cells. Our aims were: 1, to assess whether oral administration of L. brevis (CD2) affects H. pylori survival in the human gastric mucosa; 2, to evaluate the effects of L. brevis (CD2) on polyamine biosynthesis in gastric biopsies from H. pylori‐positive patients.
Materials and Methods. For 3 weeks before endoscopy, 22 H. pylori‐positive dyspeptic patients randomly received (ratio 1 : 1) high oral doses of L. brevis (CD2) or placebo. Before and after treatment, H. pylori infection was determined by urea breath test (UBT). In gastric biopsies, ornithine decarboxylase activity and polyamine levels were, respectively, evaluated by a radiometric technique and high‐pressure liquid chromatography (HPLC).
Results. L. brevis (CD2) treatment did not eradicate H. pylori. However, a reduction in the UBT delta values occurred, suggesting a decrease in intragastric bacterial load. Significantly, L. brevis (CD2) induced a decrease in gastric ornithine decarboxylase activity and polyamine levels.
Conclusions. Our data support the hypothesis that L. brevis (CD2) treatment decreases H. pylori colonization, thus reducing polyamine biosynthesis. Alternatively, the arginine deiminase activity following L. brevis (CD2) administration might cause arginine deficiency, preventing polyamine generation from gastric cells.</description><identifier>ISSN: 1083-4389</identifier><identifier>EISSN: 1523-5378</identifier><identifier>DOI: 10.1111/j.1083-4389.2004.00214.x</identifier><identifier>PMID: 15068419</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Ltd</publisher><subject>Adult ; Aged ; Biogenic Polyamines - metabolism ; Biopsy ; Breath Tests ; Double-Blind Method ; Female ; Gastric Mucosa - enzymology ; Gastric Mucosa - metabolism ; Gastric Mucosa - microbiology ; Gastroscopy ; Helicobacter Infections - metabolism ; Helicobacter Infections - microbiology ; Helicobacter pylori ; Helicobacter pylori - physiology ; Humans ; Lactobacillus - physiology ; Lactobacillus brevis ; Lactobacillus brevis (CD2) ; Male ; Middle Aged ; ornithine decarboxylase ; Ornithine Decarboxylase - metabolism ; polyamines ; Probiotics ; Urea - analysis</subject><ispartof>Helicobacter (Cambridge, Mass.), 2004-04, Vol.9 (2), p.165-172</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4994-5165d8a8debb627cb5c43fd39b964a49480fd48abe150988948e944d89d645143</citedby><cites>FETCH-LOGICAL-c4994-5165d8a8debb627cb5c43fd39b964a49480fd48abe150988948e944d89d645143</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1083-4389.2004.00214.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1083-4389.2004.00214.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15068419$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Linsalata, Michele</creatorcontrib><creatorcontrib>Russo, Francesco</creatorcontrib><creatorcontrib>Berloco, Pasquale</creatorcontrib><creatorcontrib>Caruso, Maria Lucia</creatorcontrib><creatorcontrib>Matteo, Giovanni D.I.</creatorcontrib><creatorcontrib>Cifone, Maria Grazia</creatorcontrib><creatorcontrib>Simone, Claudio D.E.</creatorcontrib><creatorcontrib>Ierardi, Enzo</creatorcontrib><creatorcontrib>Leo, Alfredo Di</creatorcontrib><title>The Influence of Lactobacillus brevis on Ornithine Decarboxylase Activity and Polyamine Profiles in Helicobacter pylori-Infected Gastric Mucosa</title><title>Helicobacter (Cambridge, Mass.)</title><addtitle>Helicobacter</addtitle><description>ABSTRACT
Background. Functional probiotics may prevent Helicobacter pylori infection, and some evidence suggests that they also possess antitumor properties. Lactobacillus brevis (CD2) is a functional Lactobacillus strain with peculiar biochemical features, essentially related to the activity of arginine deiminase. This enzyme catalyzes the catabolism of arginine and affects the biosynthesis of polyamines (putrescine, spermidine, and spermine). Polyamines are polycations found in high concentrations in both normal and neoplastic cells. Our aims were: 1, to assess whether oral administration of L. brevis (CD2) affects H. pylori survival in the human gastric mucosa; 2, to evaluate the effects of L. brevis (CD2) on polyamine biosynthesis in gastric biopsies from H. pylori‐positive patients.
Materials and Methods. For 3 weeks before endoscopy, 22 H. pylori‐positive dyspeptic patients randomly received (ratio 1 : 1) high oral doses of L. brevis (CD2) or placebo. Before and after treatment, H. pylori infection was determined by urea breath test (UBT). In gastric biopsies, ornithine decarboxylase activity and polyamine levels were, respectively, evaluated by a radiometric technique and high‐pressure liquid chromatography (HPLC).
Results. L. brevis (CD2) treatment did not eradicate H. pylori. However, a reduction in the UBT delta values occurred, suggesting a decrease in intragastric bacterial load. Significantly, L. brevis (CD2) induced a decrease in gastric ornithine decarboxylase activity and polyamine levels.
Conclusions. Our data support the hypothesis that L. brevis (CD2) treatment decreases H. pylori colonization, thus reducing polyamine biosynthesis. Alternatively, the arginine deiminase activity following L. brevis (CD2) administration might cause arginine deficiency, preventing polyamine generation from gastric cells.</description><subject>Adult</subject><subject>Aged</subject><subject>Biogenic Polyamines - metabolism</subject><subject>Biopsy</subject><subject>Breath Tests</subject><subject>Double-Blind Method</subject><subject>Female</subject><subject>Gastric Mucosa - enzymology</subject><subject>Gastric Mucosa - metabolism</subject><subject>Gastric Mucosa - microbiology</subject><subject>Gastroscopy</subject><subject>Helicobacter Infections - metabolism</subject><subject>Helicobacter Infections - microbiology</subject><subject>Helicobacter pylori</subject><subject>Helicobacter pylori - physiology</subject><subject>Humans</subject><subject>Lactobacillus - physiology</subject><subject>Lactobacillus brevis</subject><subject>Lactobacillus brevis (CD2)</subject><subject>Male</subject><subject>Middle Aged</subject><subject>ornithine decarboxylase</subject><subject>Ornithine Decarboxylase - metabolism</subject><subject>polyamines</subject><subject>Probiotics</subject><subject>Urea - analysis</subject><issn>1083-4389</issn><issn>1523-5378</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkcFu1DAQhiMEoqXwCsgnblns2E5siUsp7aZooSso4mg5zkT14o0XOymbp-CVcboLHMEXe6zv_0czf5YhghckndebBcGC5owKuSgwZguMC8IW-0fZKeEFzTmtxOP0_g2dZM9i3GCMOWXyaXZCOC4FI_I0-3l7B-i679wIvQHkO7TSZvCNNta5MaImwL2NyPfoJvR2uLM9oHdgdGj8fnI6Ajo3g723w4R036K1d5PeztA6-M46iMj2qAZnzew5QEC7yflg89QTUt2ipY5DsAZ9GI2P-nn2pNMuwovjfZZ9ubq8vajz1c3y-uJ8lRsmJcs5KXkrtGihacqiMg03jHYtlY0smWaSCdy1TOgG0qhSiPQBkrFWyLZknDB6lr06-O6C_z5CHNTWRgPO6R78GFVFBKYs7etfIKkkZwWpEigOoAk-xgCd2gW71WFSBKs5NbVRcyBqDkTNqamH1NQ-SV8ee4zNFtq_wmNMCXhzAH6klU7_bazqy1XxMGx-kNs4wP6PXIdvqqxoxdXXj0tV48_r91dvP6ma_gIADbaq</recordid><startdate>200404</startdate><enddate>200404</enddate><creator>Linsalata, Michele</creator><creator>Russo, Francesco</creator><creator>Berloco, Pasquale</creator><creator>Caruso, Maria Lucia</creator><creator>Matteo, Giovanni D.I.</creator><creator>Cifone, Maria Grazia</creator><creator>Simone, Claudio D.E.</creator><creator>Ierardi, Enzo</creator><creator>Leo, Alfredo Di</creator><general>Blackwell Science Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>200404</creationdate><title>The Influence of Lactobacillus brevis on Ornithine Decarboxylase Activity and Polyamine Profiles in Helicobacter pylori-Infected Gastric Mucosa</title><author>Linsalata, Michele ; Russo, Francesco ; Berloco, Pasquale ; Caruso, Maria Lucia ; Matteo, Giovanni D.I. ; Cifone, Maria Grazia ; Simone, Claudio D.E. ; Ierardi, Enzo ; Leo, Alfredo Di</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4994-5165d8a8debb627cb5c43fd39b964a49480fd48abe150988948e944d89d645143</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Biogenic Polyamines - metabolism</topic><topic>Biopsy</topic><topic>Breath Tests</topic><topic>Double-Blind Method</topic><topic>Female</topic><topic>Gastric Mucosa - enzymology</topic><topic>Gastric Mucosa - metabolism</topic><topic>Gastric Mucosa - microbiology</topic><topic>Gastroscopy</topic><topic>Helicobacter Infections - metabolism</topic><topic>Helicobacter Infections - microbiology</topic><topic>Helicobacter pylori</topic><topic>Helicobacter pylori - physiology</topic><topic>Humans</topic><topic>Lactobacillus - physiology</topic><topic>Lactobacillus brevis</topic><topic>Lactobacillus brevis (CD2)</topic><topic>Male</topic><topic>Middle Aged</topic><topic>ornithine decarboxylase</topic><topic>Ornithine Decarboxylase - metabolism</topic><topic>polyamines</topic><topic>Probiotics</topic><topic>Urea - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Linsalata, Michele</creatorcontrib><creatorcontrib>Russo, Francesco</creatorcontrib><creatorcontrib>Berloco, Pasquale</creatorcontrib><creatorcontrib>Caruso, Maria Lucia</creatorcontrib><creatorcontrib>Matteo, Giovanni D.I.</creatorcontrib><creatorcontrib>Cifone, Maria Grazia</creatorcontrib><creatorcontrib>Simone, Claudio D.E.</creatorcontrib><creatorcontrib>Ierardi, Enzo</creatorcontrib><creatorcontrib>Leo, Alfredo Di</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Helicobacter (Cambridge, Mass.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Linsalata, Michele</au><au>Russo, Francesco</au><au>Berloco, Pasquale</au><au>Caruso, Maria Lucia</au><au>Matteo, Giovanni D.I.</au><au>Cifone, Maria Grazia</au><au>Simone, Claudio D.E.</au><au>Ierardi, Enzo</au><au>Leo, Alfredo Di</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Influence of Lactobacillus brevis on Ornithine Decarboxylase Activity and Polyamine Profiles in Helicobacter pylori-Infected Gastric Mucosa</atitle><jtitle>Helicobacter (Cambridge, Mass.)</jtitle><addtitle>Helicobacter</addtitle><date>2004-04</date><risdate>2004</risdate><volume>9</volume><issue>2</issue><spage>165</spage><epage>172</epage><pages>165-172</pages><issn>1083-4389</issn><eissn>1523-5378</eissn><abstract>ABSTRACT
Background. Functional probiotics may prevent Helicobacter pylori infection, and some evidence suggests that they also possess antitumor properties. Lactobacillus brevis (CD2) is a functional Lactobacillus strain with peculiar biochemical features, essentially related to the activity of arginine deiminase. This enzyme catalyzes the catabolism of arginine and affects the biosynthesis of polyamines (putrescine, spermidine, and spermine). Polyamines are polycations found in high concentrations in both normal and neoplastic cells. Our aims were: 1, to assess whether oral administration of L. brevis (CD2) affects H. pylori survival in the human gastric mucosa; 2, to evaluate the effects of L. brevis (CD2) on polyamine biosynthesis in gastric biopsies from H. pylori‐positive patients.
Materials and Methods. For 3 weeks before endoscopy, 22 H. pylori‐positive dyspeptic patients randomly received (ratio 1 : 1) high oral doses of L. brevis (CD2) or placebo. Before and after treatment, H. pylori infection was determined by urea breath test (UBT). In gastric biopsies, ornithine decarboxylase activity and polyamine levels were, respectively, evaluated by a radiometric technique and high‐pressure liquid chromatography (HPLC).
Results. L. brevis (CD2) treatment did not eradicate H. pylori. However, a reduction in the UBT delta values occurred, suggesting a decrease in intragastric bacterial load. Significantly, L. brevis (CD2) induced a decrease in gastric ornithine decarboxylase activity and polyamine levels.
Conclusions. Our data support the hypothesis that L. brevis (CD2) treatment decreases H. pylori colonization, thus reducing polyamine biosynthesis. Alternatively, the arginine deiminase activity following L. brevis (CD2) administration might cause arginine deficiency, preventing polyamine generation from gastric cells.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>15068419</pmid><doi>10.1111/j.1083-4389.2004.00214.x</doi><tpages>8</tpages></addata></record> |
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| subjects | Adult Aged Biogenic Polyamines - metabolism Biopsy Breath Tests Double-Blind Method Female Gastric Mucosa - enzymology Gastric Mucosa - metabolism Gastric Mucosa - microbiology Gastroscopy Helicobacter Infections - metabolism Helicobacter Infections - microbiology Helicobacter pylori Helicobacter pylori - physiology Humans Lactobacillus - physiology Lactobacillus brevis Lactobacillus brevis (CD2) Male Middle Aged ornithine decarboxylase Ornithine Decarboxylase - metabolism polyamines Probiotics Urea - analysis |
| title | The Influence of Lactobacillus brevis on Ornithine Decarboxylase Activity and Polyamine Profiles in Helicobacter pylori-Infected Gastric Mucosa |
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