Development and validation of a liquid chromatography/tandem mass spectrometry (LC/MS/MS) method for the determination of ribavirin in human plasma and serum
A liquid chromatography/tandem mass spectrometry (LC/MS/MS) method was developed for the analysis of antiviral agent ribavirin in human plasma and serum. The samples (0.1 ml) were extracted from the matrix using a simple protein precipitation procedure. The supernatants were evaporated to dryness, r...
Gespeichert in:
| Veröffentlicht in: | Journal of pharmaceutical and biomedical analysis 2002-06, Vol.29 (1), p.83-94 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 94 |
|---|---|
| container_issue | 1 |
| container_start_page | 83 |
| container_title | Journal of pharmaceutical and biomedical analysis |
| container_volume | 29 |
| creator | Shou, Wilson Z Bu, Hai-Zhi Addison, Thomas Jiang, Xiangyu Naidong, Weng |
| description | A liquid chromatography/tandem mass spectrometry (LC/MS/MS) method was developed for the analysis of antiviral agent ribavirin in human plasma and serum. The samples (0.1 ml) were extracted from the matrix using a simple protein precipitation procedure. The supernatants were evaporated to dryness, reconstituted and injected onto the LC/MS/MS system. The chromatography separation was achieved on a silica column operated with an aqueous–organic mobile phase. The use of a silica column not only provided adequate retention for the extremely polar compound of ribavirin, but also enhanced electrospray ionization sensitivity with the use of high percentage organic solvent in the mobile phase. The method has been validated over the concentration range of 10–10 000 ng/ml ribavirin in human plasma and serum. Bamethan was used as the internal standard. The protein precipitation extraction has been automated based on 96-well format with the use of robotic liquid handlers to improve the overall throughput of the analysis. |
| doi_str_mv | 10.1016/S0731-7085(02)00003-1 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_71802347</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0731708502000031</els_id><sourcerecordid>71802347</sourcerecordid><originalsourceid>FETCH-LOGICAL-c457t-f8f1a8e8476b769651a911918386c2a3948884351d6f0960e14556da99dc63553</originalsourceid><addsrcrecordid>eNqFkc1uEzEQgC0EomngEUC-gNrDEnt_vN4TQil_UhCHgsTNmtizxGi93treSHkY3hU3idoj1kiW7G_G4_kIecXZO864WN2ytuJFy2RzxcprlldV8CdkwWVbFaWofz0liwfkglzG-CczDe_q5-SCl0yUQsgF-XuDexz85HBMFEZD9zBYA8n6kfqeAh3s3WwN1bvgHST_O8C0O6xSRtFRBzHSOKFO-RZTONCrzXr17TbHNc0HO29o7wNNO6QGEwZnx4fawW5hb4MdaY7d7GCk0wDRwbGPiGF2L8izHoaIL8_7kvz89PHH-kux-f756_rDptB106ailz0HibJuxbYVnWg4dJx3XFZS6BKqrpZS1lXDjehZJxjyummEga4zWlRNUy3J21PdKfi7GWNSzkaNwwAj-jmqlktWVnWbweYE6uBjDNirKVgH4aA4U_de1NGLuh-6YqU6elE8570-PzBvHZrHrLOIDLw5AxA1DH2AUdv4yFUtq7v8hyV5f-Iwj2NvMaioLY4ajQ1ZgzLe_qeVfxhDqsY</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>71802347</pqid></control><display><type>article</type><title>Development and validation of a liquid chromatography/tandem mass spectrometry (LC/MS/MS) method for the determination of ribavirin in human plasma and serum</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals Complete</source><creator>Shou, Wilson Z ; Bu, Hai-Zhi ; Addison, Thomas ; Jiang, Xiangyu ; Naidong, Weng</creator><creatorcontrib>Shou, Wilson Z ; Bu, Hai-Zhi ; Addison, Thomas ; Jiang, Xiangyu ; Naidong, Weng</creatorcontrib><description>A liquid chromatography/tandem mass spectrometry (LC/MS/MS) method was developed for the analysis of antiviral agent ribavirin in human plasma and serum. The samples (0.1 ml) were extracted from the matrix using a simple protein precipitation procedure. The supernatants were evaporated to dryness, reconstituted and injected onto the LC/MS/MS system. The chromatography separation was achieved on a silica column operated with an aqueous–organic mobile phase. The use of a silica column not only provided adequate retention for the extremely polar compound of ribavirin, but also enhanced electrospray ionization sensitivity with the use of high percentage organic solvent in the mobile phase. The method has been validated over the concentration range of 10–10 000 ng/ml ribavirin in human plasma and serum. Bamethan was used as the internal standard. The protein precipitation extraction has been automated based on 96-well format with the use of robotic liquid handlers to improve the overall throughput of the analysis.</description><identifier>ISSN: 0731-7085</identifier><identifier>EISSN: 1873-264X</identifier><identifier>DOI: 10.1016/S0731-7085(02)00003-1</identifier><identifier>PMID: 12062668</identifier><identifier>CODEN: JPBADA</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Analysis ; Antiviral Agents - blood ; Biological and medical sciences ; Chromatography, Liquid - methods ; General pharmacology ; Human plasma ; Human serum ; Humans ; Liquid chromatography ; Mass spectrometry ; Mass Spectrometry - methods ; Medical sciences ; Pharmacology. Drug treatments ; Reproducibility of Results ; Ribavirin ; Ribavirin - blood</subject><ispartof>Journal of pharmaceutical and biomedical analysis, 2002-06, Vol.29 (1), p.83-94</ispartof><rights>2002</rights><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c457t-f8f1a8e8476b769651a911918386c2a3948884351d6f0960e14556da99dc63553</citedby><cites>FETCH-LOGICAL-c457t-f8f1a8e8476b769651a911918386c2a3948884351d6f0960e14556da99dc63553</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0731-7085(02)00003-1$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13704984$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12062668$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shou, Wilson Z</creatorcontrib><creatorcontrib>Bu, Hai-Zhi</creatorcontrib><creatorcontrib>Addison, Thomas</creatorcontrib><creatorcontrib>Jiang, Xiangyu</creatorcontrib><creatorcontrib>Naidong, Weng</creatorcontrib><title>Development and validation of a liquid chromatography/tandem mass spectrometry (LC/MS/MS) method for the determination of ribavirin in human plasma and serum</title><title>Journal of pharmaceutical and biomedical analysis</title><addtitle>J Pharm Biomed Anal</addtitle><description>A liquid chromatography/tandem mass spectrometry (LC/MS/MS) method was developed for the analysis of antiviral agent ribavirin in human plasma and serum. The samples (0.1 ml) were extracted from the matrix using a simple protein precipitation procedure. The supernatants were evaporated to dryness, reconstituted and injected onto the LC/MS/MS system. The chromatography separation was achieved on a silica column operated with an aqueous–organic mobile phase. The use of a silica column not only provided adequate retention for the extremely polar compound of ribavirin, but also enhanced electrospray ionization sensitivity with the use of high percentage organic solvent in the mobile phase. The method has been validated over the concentration range of 10–10 000 ng/ml ribavirin in human plasma and serum. Bamethan was used as the internal standard. The protein precipitation extraction has been automated based on 96-well format with the use of robotic liquid handlers to improve the overall throughput of the analysis.</description><subject>Analysis</subject><subject>Antiviral Agents - blood</subject><subject>Biological and medical sciences</subject><subject>Chromatography, Liquid - methods</subject><subject>General pharmacology</subject><subject>Human plasma</subject><subject>Human serum</subject><subject>Humans</subject><subject>Liquid chromatography</subject><subject>Mass spectrometry</subject><subject>Mass Spectrometry - methods</subject><subject>Medical sciences</subject><subject>Pharmacology. Drug treatments</subject><subject>Reproducibility of Results</subject><subject>Ribavirin</subject><subject>Ribavirin - blood</subject><issn>0731-7085</issn><issn>1873-264X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1uEzEQgC0EomngEUC-gNrDEnt_vN4TQil_UhCHgsTNmtizxGi93treSHkY3hU3idoj1kiW7G_G4_kIecXZO864WN2ytuJFy2RzxcprlldV8CdkwWVbFaWofz0liwfkglzG-CczDe_q5-SCl0yUQsgF-XuDexz85HBMFEZD9zBYA8n6kfqeAh3s3WwN1bvgHST_O8C0O6xSRtFRBzHSOKFO-RZTONCrzXr17TbHNc0HO29o7wNNO6QGEwZnx4fawW5hb4MdaY7d7GCk0wDRwbGPiGF2L8izHoaIL8_7kvz89PHH-kux-f756_rDptB106ailz0HibJuxbYVnWg4dJx3XFZS6BKqrpZS1lXDjehZJxjyummEga4zWlRNUy3J21PdKfi7GWNSzkaNwwAj-jmqlktWVnWbweYE6uBjDNirKVgH4aA4U_de1NGLuh-6YqU6elE8570-PzBvHZrHrLOIDLw5AxA1DH2AUdv4yFUtq7v8hyV5f-Iwj2NvMaioLY4ajQ1ZgzLe_qeVfxhDqsY</recordid><startdate>20020620</startdate><enddate>20020620</enddate><creator>Shou, Wilson Z</creator><creator>Bu, Hai-Zhi</creator><creator>Addison, Thomas</creator><creator>Jiang, Xiangyu</creator><creator>Naidong, Weng</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20020620</creationdate><title>Development and validation of a liquid chromatography/tandem mass spectrometry (LC/MS/MS) method for the determination of ribavirin in human plasma and serum</title><author>Shou, Wilson Z ; Bu, Hai-Zhi ; Addison, Thomas ; Jiang, Xiangyu ; Naidong, Weng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c457t-f8f1a8e8476b769651a911918386c2a3948884351d6f0960e14556da99dc63553</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Analysis</topic><topic>Antiviral Agents - blood</topic><topic>Biological and medical sciences</topic><topic>Chromatography, Liquid - methods</topic><topic>General pharmacology</topic><topic>Human plasma</topic><topic>Human serum</topic><topic>Humans</topic><topic>Liquid chromatography</topic><topic>Mass spectrometry</topic><topic>Mass Spectrometry - methods</topic><topic>Medical sciences</topic><topic>Pharmacology. Drug treatments</topic><topic>Reproducibility of Results</topic><topic>Ribavirin</topic><topic>Ribavirin - blood</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shou, Wilson Z</creatorcontrib><creatorcontrib>Bu, Hai-Zhi</creatorcontrib><creatorcontrib>Addison, Thomas</creatorcontrib><creatorcontrib>Jiang, Xiangyu</creatorcontrib><creatorcontrib>Naidong, Weng</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of pharmaceutical and biomedical analysis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shou, Wilson Z</au><au>Bu, Hai-Zhi</au><au>Addison, Thomas</au><au>Jiang, Xiangyu</au><au>Naidong, Weng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development and validation of a liquid chromatography/tandem mass spectrometry (LC/MS/MS) method for the determination of ribavirin in human plasma and serum</atitle><jtitle>Journal of pharmaceutical and biomedical analysis</jtitle><addtitle>J Pharm Biomed Anal</addtitle><date>2002-06-20</date><risdate>2002</risdate><volume>29</volume><issue>1</issue><spage>83</spage><epage>94</epage><pages>83-94</pages><issn>0731-7085</issn><eissn>1873-264X</eissn><coden>JPBADA</coden><abstract>A liquid chromatography/tandem mass spectrometry (LC/MS/MS) method was developed for the analysis of antiviral agent ribavirin in human plasma and serum. The samples (0.1 ml) were extracted from the matrix using a simple protein precipitation procedure. The supernatants were evaporated to dryness, reconstituted and injected onto the LC/MS/MS system. The chromatography separation was achieved on a silica column operated with an aqueous–organic mobile phase. The use of a silica column not only provided adequate retention for the extremely polar compound of ribavirin, but also enhanced electrospray ionization sensitivity with the use of high percentage organic solvent in the mobile phase. The method has been validated over the concentration range of 10–10 000 ng/ml ribavirin in human plasma and serum. Bamethan was used as the internal standard. The protein precipitation extraction has been automated based on 96-well format with the use of robotic liquid handlers to improve the overall throughput of the analysis.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>12062668</pmid><doi>10.1016/S0731-7085(02)00003-1</doi><tpages>12</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0731-7085 |
| ispartof | Journal of pharmaceutical and biomedical analysis, 2002-06, Vol.29 (1), p.83-94 |
| issn | 0731-7085 1873-264X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_71802347 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Analysis Antiviral Agents - blood Biological and medical sciences Chromatography, Liquid - methods General pharmacology Human plasma Human serum Humans Liquid chromatography Mass spectrometry Mass Spectrometry - methods Medical sciences Pharmacology. Drug treatments Reproducibility of Results Ribavirin Ribavirin - blood |
| title | Development and validation of a liquid chromatography/tandem mass spectrometry (LC/MS/MS) method for the determination of ribavirin in human plasma and serum |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-27T06%3A19%3A26IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Development%20and%20validation%20of%20a%20liquid%20chromatography/tandem%20mass%20spectrometry%20(LC/MS/MS)%20method%20for%20the%20determination%20of%20ribavirin%20in%20human%20plasma%20and%20serum&rft.jtitle=Journal%20of%20pharmaceutical%20and%20biomedical%20analysis&rft.au=Shou,%20Wilson%20Z&rft.date=2002-06-20&rft.volume=29&rft.issue=1&rft.spage=83&rft.epage=94&rft.pages=83-94&rft.issn=0731-7085&rft.eissn=1873-264X&rft.coden=JPBADA&rft_id=info:doi/10.1016/S0731-7085(02)00003-1&rft_dat=%3Cproquest_cross%3E71802347%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=71802347&rft_id=info:pmid/12062668&rft_els_id=S0731708502000031&rfr_iscdi=true |