PARTIAL CHARACTERIZATION OF A TAPEWORM-SECRETED SIGNAL FACTOR INDUCING SUSTAINED SPIKE POTENTIALS IN THE SMOOTH MUSCLE OF THE RAT SMALL INTESTINE
The rat tapeworm Hymenolepis diminuta alters the myoelectric activity of the small intestine. To determine if secreted factors from the tapeworm are responsible for these alterations of intestinal smooth muscle activity, tapeworm-conditioned medium (TCM) obtained from in vitro culture was infused vi...
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| Veröffentlicht in: | The Journal of parasitology 2002-04, Vol.88 (2), p.227-231 |
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| container_title | The Journal of parasitology |
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| creator | Kroening, K. Dubear Zimmerman, Noah P Bass, Paul Oaks, John A |
| description | The rat tapeworm Hymenolepis diminuta alters the myoelectric activity of the small intestine. To determine if secreted factors from the tapeworm are responsible for these alterations of intestinal smooth muscle activity, tapeworm-conditioned medium (TCM) obtained from in vitro culture was infused via an indwelling cannula into the duodenum of an uninfected rat. Myoelectric recordings were analyzed for sustained spike potentials (SSP) and repetitive bursts of action potentials (RBAP), the previously characterized tapeworm modifications of the normal interdigestive myoelectric pattern. Results indicated that TCM initiated SSP, but not RBAP in the intestine of the uninfected rat. The SSP-inducing signal factor activity, present in TCM, was retained after boiling, prolonged freezing, proteinase treatment, and passage through a 10-kDa exclusion filter. The signal factor was soluble in the aqueous phase on lipid extraction. It was concluded that the SSP-inducing signal factor is a nonproteinaceous, heat-resistant, low–molecular weight, water soluble molecule. |
| doi_str_mv | 10.1645/0022-3395(2002)088[0227:PCOATS]2.0.CO;2 |
| format | Article |
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Dubear ; Zimmerman, Noah P ; Bass, Paul ; Oaks, John A</creator><creatorcontrib>Kroening, K. Dubear ; Zimmerman, Noah P ; Bass, Paul ; Oaks, John A</creatorcontrib><description>The rat tapeworm Hymenolepis diminuta alters the myoelectric activity of the small intestine. To determine if secreted factors from the tapeworm are responsible for these alterations of intestinal smooth muscle activity, tapeworm-conditioned medium (TCM) obtained from in vitro culture was infused via an indwelling cannula into the duodenum of an uninfected rat. Myoelectric recordings were analyzed for sustained spike potentials (SSP) and repetitive bursts of action potentials (RBAP), the previously characterized tapeworm modifications of the normal interdigestive myoelectric pattern. Results indicated that TCM initiated SSP, but not RBAP in the intestine of the uninfected rat. The SSP-inducing signal factor activity, present in TCM, was retained after boiling, prolonged freezing, proteinase treatment, and passage through a 10-kDa exclusion filter. The signal factor was soluble in the aqueous phase on lipid extraction. It was concluded that the SSP-inducing signal factor is a nonproteinaceous, heat-resistant, low–molecular weight, water soluble molecule.</description><identifier>ISSN: 0022-3395</identifier><identifier>EISSN: 1937-2345</identifier><identifier>DOI: 10.1645/0022-3395(2002)088[0227:PCOATS]2.0.CO;2</identifier><identifier>PMID: 12053990</identifier><identifier>CODEN: JOPAA2</identifier><language>eng</language><publisher>Lawrence, KS: American Society of Parasitologists</publisher><subject>Action Potentials - drug effects ; Action Potentials - physiology ; Animals ; BIOCHEMISTRY-PHYSIOLOGY ; Biological and medical sciences ; Culture Media, Conditioned ; Duodenum - drug effects ; Duodenum - physiology ; Electrical phases ; Electrodes ; Electromyography ; Fundamental and applied biological sciences. Psychology ; Host parasite relation; pathogenicity ; Hymenolepis - physiology ; Invertebrates ; Lipids ; Male ; Muscle Contraction - drug effects ; Muscle Contraction - physiology ; Muscle, Smooth - drug effects ; Muscle, Smooth - physiology ; Nemathelminthia. 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Dubear</creatorcontrib><creatorcontrib>Zimmerman, Noah P</creatorcontrib><creatorcontrib>Bass, Paul</creatorcontrib><creatorcontrib>Oaks, John A</creatorcontrib><title>PARTIAL CHARACTERIZATION OF A TAPEWORM-SECRETED SIGNAL FACTOR INDUCING SUSTAINED SPIKE POTENTIALS IN THE SMOOTH MUSCLE OF THE RAT SMALL INTESTINE</title><title>The Journal of parasitology</title><addtitle>J Parasitol</addtitle><description>The rat tapeworm Hymenolepis diminuta alters the myoelectric activity of the small intestine. To determine if secreted factors from the tapeworm are responsible for these alterations of intestinal smooth muscle activity, tapeworm-conditioned medium (TCM) obtained from in vitro culture was infused via an indwelling cannula into the duodenum of an uninfected rat. Myoelectric recordings were analyzed for sustained spike potentials (SSP) and repetitive bursts of action potentials (RBAP), the previously characterized tapeworm modifications of the normal interdigestive myoelectric pattern. Results indicated that TCM initiated SSP, but not RBAP in the intestine of the uninfected rat. The SSP-inducing signal factor activity, present in TCM, was retained after boiling, prolonged freezing, proteinase treatment, and passage through a 10-kDa exclusion filter. The signal factor was soluble in the aqueous phase on lipid extraction. It was concluded that the SSP-inducing signal factor is a nonproteinaceous, heat-resistant, low–molecular weight, water soluble molecule.</description><subject>Action Potentials - drug effects</subject><subject>Action Potentials - physiology</subject><subject>Animals</subject><subject>BIOCHEMISTRY-PHYSIOLOGY</subject><subject>Biological and medical sciences</subject><subject>Culture Media, Conditioned</subject><subject>Duodenum - drug effects</subject><subject>Duodenum - physiology</subject><subject>Electrical phases</subject><subject>Electrodes</subject><subject>Electromyography</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Host parasite relation; pathogenicity</subject><subject>Hymenolepis - physiology</subject><subject>Invertebrates</subject><subject>Lipids</subject><subject>Male</subject><subject>Muscle Contraction - drug effects</subject><subject>Muscle Contraction - physiology</subject><subject>Muscle, Smooth - drug effects</subject><subject>Muscle, Smooth - physiology</subject><subject>Nemathelminthia. Plathelmintha</subject><subject>Parasitology</subject><subject>Physiology</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Small intestine</subject><subject>Smooth muscle</subject><subject>Spike potentials</subject><subject>Tapeworms</subject><issn>0022-3395</issn><issn>1937-2345</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqdkc-O0zAYxCMEYrsLr4B8AASHdP0nTpzlZGXdNiKNo8QVEghZTppIXbXNEreHfQzeGEepWM4cLFvjn8efZjzvFsE5CgN6CyHGPiEx_YTd8TNk7IdTorsikVxVP_EczhP5Bb_wZigmkY9JQF96s7-vrrxrax8ghNSt194VwpCSOIYz73fBS5XyDCQrXvJEiTL9zlUqcyAXgAPFC_FNlmu_EkkplLgHVbrMHb5wrCxBmt9vkjRfgmpTKZ7mI1CkXwUopBL5aFw5BqiVANVaSrUC602VZGJ0H8WSK3fBs8xRSlTKObzxXnVmb9u3l_3G2yyESlZ-JpdpwjO_DkJ88oMtIzWCdYthaLDpXEqhMTU1LKIRRA1raMfqmlC2rQMc4c7lQiLY4LBhDLGO3HgfJ9_Hof91bu1JH3a2afd7c2z7s9URYhDFKHDgcgKbobd2aDv9OOwOZnjSCOqxHT3mrMec9diOdu3osR09taOdohOpsXN6d_nyXB_a7bPPpQ4HfLgAxjZm3w3m2OzsM0fCmAZR6Lj3E_dgT_3w7zyYwEgTzCgNR0xMWL3r-2P733P_AchVrqw</recordid><startdate>20020401</startdate><enddate>20020401</enddate><creator>Kroening, K. Dubear</creator><creator>Zimmerman, Noah P</creator><creator>Bass, Paul</creator><creator>Oaks, John A</creator><general>American Society of Parasitologists</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20020401</creationdate><title>PARTIAL CHARACTERIZATION OF A TAPEWORM-SECRETED SIGNAL FACTOR INDUCING SUSTAINED SPIKE POTENTIALS IN THE SMOOTH MUSCLE OF THE RAT SMALL INTESTINE</title><author>Kroening, K. Dubear ; Zimmerman, Noah P ; Bass, Paul ; Oaks, John A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b462t-4d83b10be206a2af6456aab5a875701c8c5f8bb358db4272f937370c26c8818f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Action Potentials - drug effects</topic><topic>Action Potentials - physiology</topic><topic>Animals</topic><topic>BIOCHEMISTRY-PHYSIOLOGY</topic><topic>Biological and medical sciences</topic><topic>Culture Media, Conditioned</topic><topic>Duodenum - drug effects</topic><topic>Duodenum - physiology</topic><topic>Electrical phases</topic><topic>Electrodes</topic><topic>Electromyography</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Host parasite relation; pathogenicity</topic><topic>Hymenolepis - physiology</topic><topic>Invertebrates</topic><topic>Lipids</topic><topic>Male</topic><topic>Muscle Contraction - drug effects</topic><topic>Muscle Contraction - physiology</topic><topic>Muscle, Smooth - drug effects</topic><topic>Muscle, Smooth - physiology</topic><topic>Nemathelminthia. Plathelmintha</topic><topic>Parasitology</topic><topic>Physiology</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Small intestine</topic><topic>Smooth muscle</topic><topic>Spike potentials</topic><topic>Tapeworms</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kroening, K. Dubear</creatorcontrib><creatorcontrib>Zimmerman, Noah P</creatorcontrib><creatorcontrib>Bass, Paul</creatorcontrib><creatorcontrib>Oaks, John A</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kroening, K. Dubear</au><au>Zimmerman, Noah P</au><au>Bass, Paul</au><au>Oaks, John A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>PARTIAL CHARACTERIZATION OF A TAPEWORM-SECRETED SIGNAL FACTOR INDUCING SUSTAINED SPIKE POTENTIALS IN THE SMOOTH MUSCLE OF THE RAT SMALL INTESTINE</atitle><jtitle>The Journal of parasitology</jtitle><addtitle>J Parasitol</addtitle><date>2002-04-01</date><risdate>2002</risdate><volume>88</volume><issue>2</issue><spage>227</spage><epage>231</epage><pages>227-231</pages><issn>0022-3395</issn><eissn>1937-2345</eissn><coden>JOPAA2</coden><abstract>The rat tapeworm Hymenolepis diminuta alters the myoelectric activity of the small intestine. To determine if secreted factors from the tapeworm are responsible for these alterations of intestinal smooth muscle activity, tapeworm-conditioned medium (TCM) obtained from in vitro culture was infused via an indwelling cannula into the duodenum of an uninfected rat. Myoelectric recordings were analyzed for sustained spike potentials (SSP) and repetitive bursts of action potentials (RBAP), the previously characterized tapeworm modifications of the normal interdigestive myoelectric pattern. Results indicated that TCM initiated SSP, but not RBAP in the intestine of the uninfected rat. The SSP-inducing signal factor activity, present in TCM, was retained after boiling, prolonged freezing, proteinase treatment, and passage through a 10-kDa exclusion filter. The signal factor was soluble in the aqueous phase on lipid extraction. It was concluded that the SSP-inducing signal factor is a nonproteinaceous, heat-resistant, low–molecular weight, water soluble molecule.</abstract><cop>Lawrence, KS</cop><pub>American Society of Parasitologists</pub><pmid>12053990</pmid><doi>10.1645/0022-3395(2002)088[0227:PCOATS]2.0.CO;2</doi><tpages>5</tpages></addata></record> |
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| subjects | Action Potentials - drug effects Action Potentials - physiology Animals BIOCHEMISTRY-PHYSIOLOGY Biological and medical sciences Culture Media, Conditioned Duodenum - drug effects Duodenum - physiology Electrical phases Electrodes Electromyography Fundamental and applied biological sciences. Psychology Host parasite relation pathogenicity Hymenolepis - physiology Invertebrates Lipids Male Muscle Contraction - drug effects Muscle Contraction - physiology Muscle, Smooth - drug effects Muscle, Smooth - physiology Nemathelminthia. Plathelmintha Parasitology Physiology Rats Rats, Sprague-Dawley Small intestine Smooth muscle Spike potentials Tapeworms |
| title | PARTIAL CHARACTERIZATION OF A TAPEWORM-SECRETED SIGNAL FACTOR INDUCING SUSTAINED SPIKE POTENTIALS IN THE SMOOTH MUSCLE OF THE RAT SMALL INTESTINE |
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