Equine herpesvirus 1 (EHV-1) glycoprotein D DNA inoculation in horses with pre-existing EHV-1/EHV-4 antibody
We have shown previously that equine herpesvirus 1 (EHV-1) glycoprotein D (gD) DNA elicited protective immune responses against EHV-1 challenge in murine respiratory and abortion models of EHV-1 disease. In this study, 20 horses, all with pre-existing antibody to EHV-4 and two with pre-existing anti...
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| Veröffentlicht in: | Veterinary microbiology 2000-09, Vol.76 (2), p.117-127 |
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| creator | Ruitenberg, Kirsten M Love, Daria N Gilkerson, James R Wellington, Janet E Whalley, J.Millar |
| description | We have shown previously that equine herpesvirus 1 (EHV-1) glycoprotein D (gD) DNA elicited protective immune responses against EHV-1 challenge in murine respiratory and abortion models of EHV-1 disease. In this study, 20 horses, all with pre-existing antibody to EHV-4 and two with pre-existing antibody to EHV-1, were inoculated intramuscularly with three doses each of 50, 200 or 500
μg EHV-1 gD DNA or with 500
μg vector DNA. In 8 of 15 horses, inoculation with EHV-1 gD DNA led to elevated gD-specific antibody and nine horses exhibited increased virus neutralising (VN) antibody titres compared to those present when first inoculated. A lack of increase in gC-specific antibody during the 66 weeks of the experiment showed that the increase in gD-specific antibodies was not due to a natural infection with either EHV-1 or EHV-4. The increase in EHV-1 gD-specific antibodies was predominantly an IgGa and IgGb antibody response, similar to the isotype profile reported following natural EHV-1 infection. |
| doi_str_mv | 10.1016/S0378-1135(00)00237-6 |
| format | Article |
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μg EHV-1 gD DNA or with 500
μg vector DNA. In 8 of 15 horses, inoculation with EHV-1 gD DNA led to elevated gD-specific antibody and nine horses exhibited increased virus neutralising (VN) antibody titres compared to those present when first inoculated. A lack of increase in gC-specific antibody during the 66 weeks of the experiment showed that the increase in gD-specific antibodies was not due to a natural infection with either EHV-1 or EHV-4. The increase in EHV-1 gD-specific antibodies was predominantly an IgGa and IgGb antibody response, similar to the isotype profile reported following natural EHV-1 infection.</description><identifier>ISSN: 0378-1135</identifier><identifier>EISSN: 1873-2542</identifier><identifier>DOI: 10.1016/S0378-1135(00)00237-6</identifier><identifier>PMID: 10946142</identifier><identifier>CODEN: VMICDQ</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animals ; Antibodies, Viral - analysis ; Biological and medical sciences ; Blotting, Western - veterinary ; DNA immunisation ; Enzyme-Linked Immunosorbent Assay - veterinary ; Equine herpesvirus 1 ; Equine herpesvirus 1 (EHV-1) ; Equine herpesvirus 4 ; Fundamental and applied biological sciences. Psychology ; Glycoprotein D ; Hemagglutinins, Viral - immunology ; Herpesviridae Infections - prevention & control ; Herpesviridae Infections - veterinary ; Herpesvirus 1, Equid - immunology ; Herpesvirus 4, Equid - immunology ; Horse Diseases - prevention & control ; Horses ; Microbiology ; Neutralization Tests - veterinary ; Vaccination - veterinary ; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies ; Vaccines, DNA ; Viral Envelope Proteins - immunology ; Virology</subject><ispartof>Veterinary microbiology, 2000-09, Vol.76 (2), p.117-127</ispartof><rights>2000 Elsevier Science B.V.</rights><rights>2001 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c420t-bced5ce488cee60d944dfa78f7c86376a132f9220a559b95d28b359fc954a8013</citedby><cites>FETCH-LOGICAL-c420t-bced5ce488cee60d944dfa78f7c86376a132f9220a559b95d28b359fc954a8013</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0378-1135(00)00237-6$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,27929,27930,46000</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=918993$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10946142$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ruitenberg, Kirsten M</creatorcontrib><creatorcontrib>Love, Daria N</creatorcontrib><creatorcontrib>Gilkerson, James R</creatorcontrib><creatorcontrib>Wellington, Janet E</creatorcontrib><creatorcontrib>Whalley, J.Millar</creatorcontrib><title>Equine herpesvirus 1 (EHV-1) glycoprotein D DNA inoculation in horses with pre-existing EHV-1/EHV-4 antibody</title><title>Veterinary microbiology</title><addtitle>Vet Microbiol</addtitle><description>We have shown previously that equine herpesvirus 1 (EHV-1) glycoprotein D (gD) DNA elicited protective immune responses against EHV-1 challenge in murine respiratory and abortion models of EHV-1 disease. In this study, 20 horses, all with pre-existing antibody to EHV-4 and two with pre-existing antibody to EHV-1, were inoculated intramuscularly with three doses each of 50, 200 or 500
μg EHV-1 gD DNA or with 500
μg vector DNA. In 8 of 15 horses, inoculation with EHV-1 gD DNA led to elevated gD-specific antibody and nine horses exhibited increased virus neutralising (VN) antibody titres compared to those present when first inoculated. A lack of increase in gC-specific antibody during the 66 weeks of the experiment showed that the increase in gD-specific antibodies was not due to a natural infection with either EHV-1 or EHV-4. The increase in EHV-1 gD-specific antibodies was predominantly an IgGa and IgGb antibody response, similar to the isotype profile reported following natural EHV-1 infection.</description><subject>Animals</subject><subject>Antibodies, Viral - analysis</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western - veterinary</subject><subject>DNA immunisation</subject><subject>Enzyme-Linked Immunosorbent Assay - veterinary</subject><subject>Equine herpesvirus 1</subject><subject>Equine herpesvirus 1 (EHV-1)</subject><subject>Equine herpesvirus 4</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glycoprotein D</subject><subject>Hemagglutinins, Viral - immunology</subject><subject>Herpesviridae Infections - prevention & control</subject><subject>Herpesviridae Infections - veterinary</subject><subject>Herpesvirus 1, Equid - immunology</subject><subject>Herpesvirus 4, Equid - immunology</subject><subject>Horse Diseases - prevention & control</subject><subject>Horses</subject><subject>Microbiology</subject><subject>Neutralization Tests - veterinary</subject><subject>Vaccination - veterinary</subject><subject>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies</subject><subject>Vaccines, DNA</subject><subject>Viral Envelope Proteins - immunology</subject><subject>Virology</subject><issn>0378-1135</issn><issn>1873-2542</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1P3DAQhq2qqGxpf0IrS5UqOKSMP2LHJ4RgC5UQHAq9Wo4zYV1l48VOaPffN_sh1BuXmTk8M_PqfQn5xOAbA6ZOf4LQVcGYKI8BTgC40IV6Q2as0qLgpeRvyewFOSTvc_4NANIoeEcOGRipmOQz0s2fxtAjXWBaYX4OacyU0eP59a-CndDHbu3jKsUBQ08v6eXtOQ199GPnhhD7aaaLmDJm-icMC7pKWODfkIfQP9LthdNNldT1Q6hjs_5ADlrXZfy470fk4fv8_uK6uLm7-nFxflN4yWEoao9N6VFWlUdU0Bgpm9bpqtW-UkIrxwRvDefgytLUpmx4VYvStN6U0lXAxBH5urs7SX8aMQ92GbLHrnM9xjFbzbQxyrwOMq2ZFAATWO5An2LOCVu7SmHp0toysJs87DYPuzHbAthtHlZNe5_3D8Z6ic1_W7sAJuDLHnDZu65Nrvchv3CGVcaIiTrbUTi59hww2ewD9pNPIaEfbBPDK0L-ATKIpI8</recordid><startdate>20000925</startdate><enddate>20000925</enddate><creator>Ruitenberg, Kirsten M</creator><creator>Love, Daria N</creator><creator>Gilkerson, James R</creator><creator>Wellington, Janet E</creator><creator>Whalley, J.Millar</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20000925</creationdate><title>Equine herpesvirus 1 (EHV-1) glycoprotein D DNA inoculation in horses with pre-existing EHV-1/EHV-4 antibody</title><author>Ruitenberg, Kirsten M ; Love, Daria N ; Gilkerson, James R ; Wellington, Janet E ; Whalley, J.Millar</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c420t-bced5ce488cee60d944dfa78f7c86376a132f9220a559b95d28b359fc954a8013</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Animals</topic><topic>Antibodies, Viral - analysis</topic><topic>Biological and medical sciences</topic><topic>Blotting, Western - veterinary</topic><topic>DNA immunisation</topic><topic>Enzyme-Linked Immunosorbent Assay - veterinary</topic><topic>Equine herpesvirus 1</topic><topic>Equine herpesvirus 1 (EHV-1)</topic><topic>Equine herpesvirus 4</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glycoprotein D</topic><topic>Hemagglutinins, Viral - immunology</topic><topic>Herpesviridae Infections - prevention & control</topic><topic>Herpesviridae Infections - veterinary</topic><topic>Herpesvirus 1, Equid - immunology</topic><topic>Herpesvirus 4, Equid - immunology</topic><topic>Horse Diseases - prevention & control</topic><topic>Horses</topic><topic>Microbiology</topic><topic>Neutralization Tests - veterinary</topic><topic>Vaccination - veterinary</topic><topic>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies</topic><topic>Vaccines, DNA</topic><topic>Viral Envelope Proteins - immunology</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ruitenberg, Kirsten M</creatorcontrib><creatorcontrib>Love, Daria N</creatorcontrib><creatorcontrib>Gilkerson, James R</creatorcontrib><creatorcontrib>Wellington, Janet E</creatorcontrib><creatorcontrib>Whalley, J.Millar</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ruitenberg, Kirsten M</au><au>Love, Daria N</au><au>Gilkerson, James R</au><au>Wellington, Janet E</au><au>Whalley, J.Millar</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Equine herpesvirus 1 (EHV-1) glycoprotein D DNA inoculation in horses with pre-existing EHV-1/EHV-4 antibody</atitle><jtitle>Veterinary microbiology</jtitle><addtitle>Vet Microbiol</addtitle><date>2000-09-25</date><risdate>2000</risdate><volume>76</volume><issue>2</issue><spage>117</spage><epage>127</epage><pages>117-127</pages><issn>0378-1135</issn><eissn>1873-2542</eissn><coden>VMICDQ</coden><abstract>We have shown previously that equine herpesvirus 1 (EHV-1) glycoprotein D (gD) DNA elicited protective immune responses against EHV-1 challenge in murine respiratory and abortion models of EHV-1 disease. In this study, 20 horses, all with pre-existing antibody to EHV-4 and two with pre-existing antibody to EHV-1, were inoculated intramuscularly with three doses each of 50, 200 or 500
μg EHV-1 gD DNA or with 500
μg vector DNA. In 8 of 15 horses, inoculation with EHV-1 gD DNA led to elevated gD-specific antibody and nine horses exhibited increased virus neutralising (VN) antibody titres compared to those present when first inoculated. A lack of increase in gC-specific antibody during the 66 weeks of the experiment showed that the increase in gD-specific antibodies was not due to a natural infection with either EHV-1 or EHV-4. The increase in EHV-1 gD-specific antibodies was predominantly an IgGa and IgGb antibody response, similar to the isotype profile reported following natural EHV-1 infection.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>10946142</pmid><doi>10.1016/S0378-1135(00)00237-6</doi><tpages>11</tpages></addata></record> |
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| subjects | Animals Antibodies, Viral - analysis Biological and medical sciences Blotting, Western - veterinary DNA immunisation Enzyme-Linked Immunosorbent Assay - veterinary Equine herpesvirus 1 Equine herpesvirus 1 (EHV-1) Equine herpesvirus 4 Fundamental and applied biological sciences. Psychology Glycoprotein D Hemagglutinins, Viral - immunology Herpesviridae Infections - prevention & control Herpesviridae Infections - veterinary Herpesvirus 1, Equid - immunology Herpesvirus 4, Equid - immunology Horse Diseases - prevention & control Horses Microbiology Neutralization Tests - veterinary Vaccination - veterinary Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies Vaccines, DNA Viral Envelope Proteins - immunology Virology |
| title | Equine herpesvirus 1 (EHV-1) glycoprotein D DNA inoculation in horses with pre-existing EHV-1/EHV-4 antibody |
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