Brugia malayi: transient transfection by microinjection and particle bombardment

To develop a method for the introduction of DNA into filarial parasites, several methods that have proven successful in other organisms were evaluated for their ability to transform Brugia malayi. Luciferase activity was detectable in embryos bombarded with gold particles coated with a construct con...

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Veröffentlicht in:	Experimental parasitology 2002-02, Vol.100 (2), p.95-102
Hauptverfasser:	Higazi, Tarig B., Merriweather, Anthony, Shu, Limin, Davis, Richard, Unnasch, Thomas R.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Base Sequence Biochemistry. Physiology. Immunology. Molecular biology Biolistics Biological and medical sciences Biotechnology Brugia malayi - enzymology Brugia malayi - genetics Brugia malayi - metabolism DNA, Helminth - chemistry DNA, Helminth - genetics Female Fundamental and applied biological sciences. Psychology Gene Expression Regulation Gene transfer Genes, Reporter Genetic engineering Genetic technics Green Fluorescent Proteins Invertebrates Luciferases - genetics Luciferases - metabolism Luminescent Proteins - genetics Luminescent Proteins - metabolism Methods. Procedures. Technologies Microinjections Microspheres Molecular Sequence Data Nemathelminthia. Plathelmintha Physiology. Development Plasmids - genetics Promoter Regions, Genetic - genetics RNA, Ribosomal, 5S - chemistry RNA, Ribosomal, 5S - genetics RNA, Spliced Leader - genetics Synthetic digonucleotides and genes. Sequencing Transfection - methods
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