Deep Knot Structure for Construction of Active Site and Cofactor Binding Site of tRNA Modification Enzyme

The tRNA(Gm18) methyltransferase (TrmH) catalyzes the 2′- O methylation of guanosine 18 (Gua18) of tRNA. We solved the crystal structure of Thermus thermophilus TrmH complexed with S-adenosyl-L-methionine at 1.85 Å resolution. The catalytic domain contains a deep trefoil knot, which mutational analy...

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Veröffentlicht in:	Structure (London) 2004-04, Vol.12 (4), p.593-602
Hauptverfasser:	Nureki, Osamu, Watanabe, Kazunori, Fukai, Shuya, Ishii, Ryohei, Endo, Yaeta, Hori, Hiroyuki, Yokoyama, Shigeyuki
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Binding Sites Catalytic Domain Coenzymes - metabolism Dimerization Kinetics Molecular Sequence Data Protein Structure, Tertiary S-Adenosylmethionine - metabolism Thermus thermophilus - enzymology Thermus thermophilus - genetics Thermus thermophilus - metabolism tRNA Methyltransferases - chemistry tRNA Methyltransferases - genetics tRNA Methyltransferases - metabolism
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