Preparative Purification of a Recombinant Protein by Hydrophobic Interaction Chromatography: Modulation of Selectivity by the Use of Chaotropic Additives

Development and implementation of a chaotropic wash step following protein loading on a hydrophobic interaction chromatographic (HIC) column is described for the purification of a recombinant protein. Various agents that reduce protein affinity in hydrophobic interaction chromatographic systems were...

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Veröffentlicht in:	Biotechnology progress 2002, Vol.18 (3), p.556-564
Hauptverfasser:	Shukla, Abhinav A., Peterson, Joshua, Sorge, Laura, Lewis, Patsy, Thomas, Shannon, Waugh, Steve
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biotechnology Chromatography, Liquid - methods Electrophoresis, Polyacrylamide Gel Fundamental and applied biological sciences. Psychology Methods. Procedures. Technologies Others Recombinant Proteins - isolation & purification Sensitivity and Specificity Spectrophotometry, Ultraviolet Various methods and equipments
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