Overexpression of glia maturation factor in astrocytes leads to immune activation of microglia through secretion of granulocyte–macrophage-colony stimulating factor

We infected a mixed culture of primary rat astrocytes and microglia with a replication-defective adenovirus carrying the rat glia maturation factor (GMF) cDNA. Affymetrix microarray analysis showed a big increase in the expression of several major histocompatibility complex (MHC) class II proteins a...

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Veröffentlicht in:	Biochemical and biophysical research communications 2002-06, Vol.294 (2), p.238-244
Hauptverfasser:	Zaheer, Asgar, Mathur, Satya N, Lim, Ramon
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenoviridae - genetics Adenovirus Animals Astrocytes Astrocytes - cytology Astrocytes - metabolism Astrocytes - secretion Cells, Cultured Coculture Techniques Culture Media, Conditioned - chemistry Enzyme Inhibitors - pharmacology Gene Expression Profiling Glia maturation factor Glia Maturation Factor - biosynthesis Glia Maturation Factor - genetics Granulocyte-Macrophage Colony-Stimulating Factor - analysis Granulocyte-Macrophage Colony-Stimulating Factor - biosynthesis Granulocyte-Macrophage Colony-Stimulating Factor - secretion Granulocyte–macrophage-colony stimulating factor Histocompatibility Antigens Class II - genetics Immunoblotting Interleukin-1 - genetics Interleukin-1β Major histocompatibility complex class II Microglia Microglia - immunology Microglia - metabolism Oligonucleotide Array Sequence Analysis Rats Reverse Transcriptase Polymerase Chain Reaction Transfection
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