Leukemia inhibitory factor relaxes arteries through endothelium-dependent mechanism
Leukemia inhibitory factor (LIF) is a cytokine, which inhibits angiogenesis and decreases endothelial cell proliferation and migration, suggesting that LIF may modulate vascular tone. In this study, we examined the effects of LIF on the tone of rat arteries. The isometric tension of ring preparation...
Gespeichert in:
| Veröffentlicht in: | Biochemical and biophysical research communications 2002-06, Vol.294 (2), p.359-362 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 362 |
|---|---|
| container_issue | 2 |
| container_start_page | 359 |
| container_title | Biochemical and biophysical research communications |
| container_volume | 294 |
| creator | Kimura, Keizo Tsuda, Kazushi Moriwaki, Chizu Kawabe, Tetsuya Hamada, Masanori Obana, Masahiro Baba, Akira Hano, Takuzo Nishio, Ichiro |
| description | Leukemia inhibitory factor (LIF) is a cytokine, which inhibits angiogenesis and decreases endothelial cell proliferation and migration, suggesting that LIF may modulate vascular tone. In this study, we examined the effects of LIF on the tone of rat arteries. The isometric tension of ring preparations from rat superior mesenteric arteries was continuously measured. LIF relaxed the mesenteric arteries in a dose-dependent manner, when the arterial rings were precontracted with phenylephrine. The relaxation was totally inhibited by mechanical removal of endothelium.
N
G-nitro-
l-arginine methyl ester did not affect the relaxation by LIF. Ca
2+-dependent K channel (KCa) blockers, apamin with charybdotoxin, inhibited the relaxation by LIF. Catalase, an enzyme which scavenges hydrogen peroxide, also inhibited the relaxation by LIF. Endothelium-derived hyperpolarizing factor relaxes smooth muscle cells and the effect is blocked by KCa and catalase. Our results suggest that LIF regulates vascular tone through the effect of this factor. |
| doi_str_mv | 10.1016/S0006-291X(02)00493-X |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_71795245</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0006291X0200493X</els_id><sourcerecordid>19722856</sourcerecordid><originalsourceid>FETCH-LOGICAL-c392t-195f5f3afcb929100c09efd8aa824fe9c2e4be4a3f0a005a149149b184caf1333</originalsourceid><addsrcrecordid>eNqFkEtLxDAQgIMouj5-gtKT6KE6k6a7m5OI-IIFDyrsLaTpxEb7WJNW9N8b3UWPCwMzA988-Bg7RDhDwPH5IwCMUy5xfgL8FEDILJ1vsBGChJQjiE02-kN22G4IrwCIYiy32Q5yyHHCYcQeZzS8UeN04trKFa7v_FditYk58VTrTwqJ9j15F4u-8t3wUiXUll1fUe2GJi1pEVtq-6QhU-nWhWafbVldBzpY5T32fHP9dHWXzh5u768uZ6nJJO9TlLnNbaatKWR8EsCAJFtOtZ5yYUkaTqIgoTMLGiDXKGSMAqfCaItZlu2x4-Xehe_eBwq9alwwVNe6pW4IaoITmXORrwVRTjif5uMI5kvQ-C4ET1YtvGu0_1II6ke7-tWufpwq4OpXu5rHuaPVgaFoqPyfWnmOwMUSoOjjw5FXwThqDZXOk-lV2bk1J74BKu2UCQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>19722856</pqid></control><display><type>article</type><title>Leukemia inhibitory factor relaxes arteries through endothelium-dependent mechanism</title><source>MEDLINE</source><source>Access via ScienceDirect (Elsevier)</source><creator>Kimura, Keizo ; Tsuda, Kazushi ; Moriwaki, Chizu ; Kawabe, Tetsuya ; Hamada, Masanori ; Obana, Masahiro ; Baba, Akira ; Hano, Takuzo ; Nishio, Ichiro</creator><creatorcontrib>Kimura, Keizo ; Tsuda, Kazushi ; Moriwaki, Chizu ; Kawabe, Tetsuya ; Hamada, Masanori ; Obana, Masahiro ; Baba, Akira ; Hano, Takuzo ; Nishio, Ichiro</creatorcontrib><description>Leukemia inhibitory factor (LIF) is a cytokine, which inhibits angiogenesis and decreases endothelial cell proliferation and migration, suggesting that LIF may modulate vascular tone. In this study, we examined the effects of LIF on the tone of rat arteries. The isometric tension of ring preparations from rat superior mesenteric arteries was continuously measured. LIF relaxed the mesenteric arteries in a dose-dependent manner, when the arterial rings were precontracted with phenylephrine. The relaxation was totally inhibited by mechanical removal of endothelium.
N
G-nitro-
l-arginine methyl ester did not affect the relaxation by LIF. Ca
2+-dependent K channel (KCa) blockers, apamin with charybdotoxin, inhibited the relaxation by LIF. Catalase, an enzyme which scavenges hydrogen peroxide, also inhibited the relaxation by LIF. Endothelium-derived hyperpolarizing factor relaxes smooth muscle cells and the effect is blocked by KCa and catalase. Our results suggest that LIF regulates vascular tone through the effect of this factor.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/S0006-291X(02)00493-X</identifier><identifier>PMID: 12051720</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Aorta - drug effects ; Aorta - physiology ; Apamin ; Biological Factors - antagonists & inhibitors ; Biological Factors - metabolism ; Calcium Channel Blockers - pharmacology ; Catalase ; Catalase - pharmacology ; Charybdotoxin ; Cytokine ; Dose-Response Relationship, Drug ; Endothelium, Vascular - drug effects ; Endothelium, Vascular - metabolism ; Endothelium-dependent hyperpolarizing factor ; Enzyme Inhibitors - pharmacology ; Growth Inhibitors - antagonists & inhibitors ; Growth Inhibitors - pharmacology ; Histamine H1 Antagonists - pharmacology ; Hydrogen peroxide ; Hydrogen Peroxide - antagonists & inhibitors ; Hydrogen Peroxide - metabolism ; In Vitro Techniques ; Interleukin-6 ; Leukemia Inhibitory Factor ; Lymphokines - antagonists & inhibitors ; Lymphokines - pharmacology ; Male ; Mesenteric Artery, Superior - drug effects ; Mesenteric Artery, Superior - physiology ; Muscarinic Antagonists - pharmacology ; NG-Nitroarginine Methyl Ester - pharmacology ; Phenylephrine - pharmacology ; Rats ; Rats, Wistar ; Tyrphostins - pharmacology ; Vasoconstrictor Agents - pharmacology ; Vasodilation - drug effects ; Vasodilation - physiology</subject><ispartof>Biochemical and biophysical research communications, 2002-06, Vol.294 (2), p.359-362</ispartof><rights>2002 Elsevier Science (USA)</rights><rights>(c) 2002 Elsevier Science (USA).</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c392t-195f5f3afcb929100c09efd8aa824fe9c2e4be4a3f0a005a149149b184caf1333</citedby><cites>FETCH-LOGICAL-c392t-195f5f3afcb929100c09efd8aa824fe9c2e4be4a3f0a005a149149b184caf1333</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0006-291X(02)00493-X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,27928,27929,45999</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12051720$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kimura, Keizo</creatorcontrib><creatorcontrib>Tsuda, Kazushi</creatorcontrib><creatorcontrib>Moriwaki, Chizu</creatorcontrib><creatorcontrib>Kawabe, Tetsuya</creatorcontrib><creatorcontrib>Hamada, Masanori</creatorcontrib><creatorcontrib>Obana, Masahiro</creatorcontrib><creatorcontrib>Baba, Akira</creatorcontrib><creatorcontrib>Hano, Takuzo</creatorcontrib><creatorcontrib>Nishio, Ichiro</creatorcontrib><title>Leukemia inhibitory factor relaxes arteries through endothelium-dependent mechanism</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Leukemia inhibitory factor (LIF) is a cytokine, which inhibits angiogenesis and decreases endothelial cell proliferation and migration, suggesting that LIF may modulate vascular tone. In this study, we examined the effects of LIF on the tone of rat arteries. The isometric tension of ring preparations from rat superior mesenteric arteries was continuously measured. LIF relaxed the mesenteric arteries in a dose-dependent manner, when the arterial rings were precontracted with phenylephrine. The relaxation was totally inhibited by mechanical removal of endothelium.
N
G-nitro-
l-arginine methyl ester did not affect the relaxation by LIF. Ca
2+-dependent K channel (KCa) blockers, apamin with charybdotoxin, inhibited the relaxation by LIF. Catalase, an enzyme which scavenges hydrogen peroxide, also inhibited the relaxation by LIF. Endothelium-derived hyperpolarizing factor relaxes smooth muscle cells and the effect is blocked by KCa and catalase. Our results suggest that LIF regulates vascular tone through the effect of this factor.</description><subject>Animals</subject><subject>Aorta - drug effects</subject><subject>Aorta - physiology</subject><subject>Apamin</subject><subject>Biological Factors - antagonists & inhibitors</subject><subject>Biological Factors - metabolism</subject><subject>Calcium Channel Blockers - pharmacology</subject><subject>Catalase</subject><subject>Catalase - pharmacology</subject><subject>Charybdotoxin</subject><subject>Cytokine</subject><subject>Dose-Response Relationship, Drug</subject><subject>Endothelium, Vascular - drug effects</subject><subject>Endothelium, Vascular - metabolism</subject><subject>Endothelium-dependent hyperpolarizing factor</subject><subject>Enzyme Inhibitors - pharmacology</subject><subject>Growth Inhibitors - antagonists & inhibitors</subject><subject>Growth Inhibitors - pharmacology</subject><subject>Histamine H1 Antagonists - pharmacology</subject><subject>Hydrogen peroxide</subject><subject>Hydrogen Peroxide - antagonists & inhibitors</subject><subject>Hydrogen Peroxide - metabolism</subject><subject>In Vitro Techniques</subject><subject>Interleukin-6</subject><subject>Leukemia Inhibitory Factor</subject><subject>Lymphokines - antagonists & inhibitors</subject><subject>Lymphokines - pharmacology</subject><subject>Male</subject><subject>Mesenteric Artery, Superior - drug effects</subject><subject>Mesenteric Artery, Superior - physiology</subject><subject>Muscarinic Antagonists - pharmacology</subject><subject>NG-Nitroarginine Methyl Ester - pharmacology</subject><subject>Phenylephrine - pharmacology</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Tyrphostins - pharmacology</subject><subject>Vasoconstrictor Agents - pharmacology</subject><subject>Vasodilation - drug effects</subject><subject>Vasodilation - physiology</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtLxDAQgIMouj5-gtKT6KE6k6a7m5OI-IIFDyrsLaTpxEb7WJNW9N8b3UWPCwMzA988-Bg7RDhDwPH5IwCMUy5xfgL8FEDILJ1vsBGChJQjiE02-kN22G4IrwCIYiy32Q5yyHHCYcQeZzS8UeN04trKFa7v_FditYk58VTrTwqJ9j15F4u-8t3wUiXUll1fUe2GJi1pEVtq-6QhU-nWhWafbVldBzpY5T32fHP9dHWXzh5u768uZ6nJJO9TlLnNbaatKWR8EsCAJFtOtZ5yYUkaTqIgoTMLGiDXKGSMAqfCaItZlu2x4-Xehe_eBwq9alwwVNe6pW4IaoITmXORrwVRTjif5uMI5kvQ-C4ET1YtvGu0_1II6ke7-tWufpwq4OpXu5rHuaPVgaFoqPyfWnmOwMUSoOjjw5FXwThqDZXOk-lV2bk1J74BKu2UCQ</recordid><startdate>20020607</startdate><enddate>20020607</enddate><creator>Kimura, Keizo</creator><creator>Tsuda, Kazushi</creator><creator>Moriwaki, Chizu</creator><creator>Kawabe, Tetsuya</creator><creator>Hamada, Masanori</creator><creator>Obana, Masahiro</creator><creator>Baba, Akira</creator><creator>Hano, Takuzo</creator><creator>Nishio, Ichiro</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20020607</creationdate><title>Leukemia inhibitory factor relaxes arteries through endothelium-dependent mechanism</title><author>Kimura, Keizo ; Tsuda, Kazushi ; Moriwaki, Chizu ; Kawabe, Tetsuya ; Hamada, Masanori ; Obana, Masahiro ; Baba, Akira ; Hano, Takuzo ; Nishio, Ichiro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c392t-195f5f3afcb929100c09efd8aa824fe9c2e4be4a3f0a005a149149b184caf1333</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Animals</topic><topic>Aorta - drug effects</topic><topic>Aorta - physiology</topic><topic>Apamin</topic><topic>Biological Factors - antagonists & inhibitors</topic><topic>Biological Factors - metabolism</topic><topic>Calcium Channel Blockers - pharmacology</topic><topic>Catalase</topic><topic>Catalase - pharmacology</topic><topic>Charybdotoxin</topic><topic>Cytokine</topic><topic>Dose-Response Relationship, Drug</topic><topic>Endothelium, Vascular - drug effects</topic><topic>Endothelium, Vascular - metabolism</topic><topic>Endothelium-dependent hyperpolarizing factor</topic><topic>Enzyme Inhibitors - pharmacology</topic><topic>Growth Inhibitors - antagonists & inhibitors</topic><topic>Growth Inhibitors - pharmacology</topic><topic>Histamine H1 Antagonists - pharmacology</topic><topic>Hydrogen peroxide</topic><topic>Hydrogen Peroxide - antagonists & inhibitors</topic><topic>Hydrogen Peroxide - metabolism</topic><topic>In Vitro Techniques</topic><topic>Interleukin-6</topic><topic>Leukemia Inhibitory Factor</topic><topic>Lymphokines - antagonists & inhibitors</topic><topic>Lymphokines - pharmacology</topic><topic>Male</topic><topic>Mesenteric Artery, Superior - drug effects</topic><topic>Mesenteric Artery, Superior - physiology</topic><topic>Muscarinic Antagonists - pharmacology</topic><topic>NG-Nitroarginine Methyl Ester - pharmacology</topic><topic>Phenylephrine - pharmacology</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Tyrphostins - pharmacology</topic><topic>Vasoconstrictor Agents - pharmacology</topic><topic>Vasodilation - drug effects</topic><topic>Vasodilation - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kimura, Keizo</creatorcontrib><creatorcontrib>Tsuda, Kazushi</creatorcontrib><creatorcontrib>Moriwaki, Chizu</creatorcontrib><creatorcontrib>Kawabe, Tetsuya</creatorcontrib><creatorcontrib>Hamada, Masanori</creatorcontrib><creatorcontrib>Obana, Masahiro</creatorcontrib><creatorcontrib>Baba, Akira</creatorcontrib><creatorcontrib>Hano, Takuzo</creatorcontrib><creatorcontrib>Nishio, Ichiro</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kimura, Keizo</au><au>Tsuda, Kazushi</au><au>Moriwaki, Chizu</au><au>Kawabe, Tetsuya</au><au>Hamada, Masanori</au><au>Obana, Masahiro</au><au>Baba, Akira</au><au>Hano, Takuzo</au><au>Nishio, Ichiro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Leukemia inhibitory factor relaxes arteries through endothelium-dependent mechanism</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2002-06-07</date><risdate>2002</risdate><volume>294</volume><issue>2</issue><spage>359</spage><epage>362</epage><pages>359-362</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>Leukemia inhibitory factor (LIF) is a cytokine, which inhibits angiogenesis and decreases endothelial cell proliferation and migration, suggesting that LIF may modulate vascular tone. In this study, we examined the effects of LIF on the tone of rat arteries. The isometric tension of ring preparations from rat superior mesenteric arteries was continuously measured. LIF relaxed the mesenteric arteries in a dose-dependent manner, when the arterial rings were precontracted with phenylephrine. The relaxation was totally inhibited by mechanical removal of endothelium.
N
G-nitro-
l-arginine methyl ester did not affect the relaxation by LIF. Ca
2+-dependent K channel (KCa) blockers, apamin with charybdotoxin, inhibited the relaxation by LIF. Catalase, an enzyme which scavenges hydrogen peroxide, also inhibited the relaxation by LIF. Endothelium-derived hyperpolarizing factor relaxes smooth muscle cells and the effect is blocked by KCa and catalase. Our results suggest that LIF regulates vascular tone through the effect of this factor.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>12051720</pmid><doi>10.1016/S0006-291X(02)00493-X</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0006-291X |
| ispartof | Biochemical and biophysical research communications, 2002-06, Vol.294 (2), p.359-362 |
| issn | 0006-291X 1090-2104 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_71795245 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Animals Aorta - drug effects Aorta - physiology Apamin Biological Factors - antagonists & inhibitors Biological Factors - metabolism Calcium Channel Blockers - pharmacology Catalase Catalase - pharmacology Charybdotoxin Cytokine Dose-Response Relationship, Drug Endothelium, Vascular - drug effects Endothelium, Vascular - metabolism Endothelium-dependent hyperpolarizing factor Enzyme Inhibitors - pharmacology Growth Inhibitors - antagonists & inhibitors Growth Inhibitors - pharmacology Histamine H1 Antagonists - pharmacology Hydrogen peroxide Hydrogen Peroxide - antagonists & inhibitors Hydrogen Peroxide - metabolism In Vitro Techniques Interleukin-6 Leukemia Inhibitory Factor Lymphokines - antagonists & inhibitors Lymphokines - pharmacology Male Mesenteric Artery, Superior - drug effects Mesenteric Artery, Superior - physiology Muscarinic Antagonists - pharmacology NG-Nitroarginine Methyl Ester - pharmacology Phenylephrine - pharmacology Rats Rats, Wistar Tyrphostins - pharmacology Vasoconstrictor Agents - pharmacology Vasodilation - drug effects Vasodilation - physiology |
| title | Leukemia inhibitory factor relaxes arteries through endothelium-dependent mechanism |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-17T01%3A29%3A46IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Leukemia%20inhibitory%20factor%20relaxes%20arteries%20through%20endothelium-dependent%20mechanism&rft.jtitle=Biochemical%20and%20biophysical%20research%20communications&rft.au=Kimura,%20Keizo&rft.date=2002-06-07&rft.volume=294&rft.issue=2&rft.spage=359&rft.epage=362&rft.pages=359-362&rft.issn=0006-291X&rft.eissn=1090-2104&rft_id=info:doi/10.1016/S0006-291X(02)00493-X&rft_dat=%3Cproquest_cross%3E19722856%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=19722856&rft_id=info:pmid/12051720&rft_els_id=S0006291X0200493X&rfr_iscdi=true |