Inhibitory Effects of α-Arbutin on Melanin Synthesis in Cultured Human Melanoma Cells and a Three-Dimensional Human Skin Model
We studied the inhibitory effects of 4-hydroxyphenyl α-glucopyranoside (α-arbutin) on melanogenesis in cultured human melanoma cells, HMV-II, and in a three-dimensional cultured human skin model. α-Arbutin showed no inhibitory effect on HMV-II cell growth at a concentration below 1.0 mM. Melanin syn...
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| Veröffentlicht in: | Biological & pharmaceutical bulletin 2004, Vol.27(4), pp.510-514 |
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| creator | Sugimoto, Kazuhisa Nishimura, Takahisa Nomura, Koji Sugimoto, Kenji Kuriki, Takashi |
| description | We studied the inhibitory effects of 4-hydroxyphenyl α-glucopyranoside (α-arbutin) on melanogenesis in cultured human melanoma cells, HMV-II, and in a three-dimensional cultured human skin model. α-Arbutin showed no inhibitory effect on HMV-II cell growth at a concentration below 1.0 mM. Melanin synthesis in cells treated with α-arbutin at 0.5 mM decreased to 76% of that in non-treated cells. The cellular tyrosinase activity of HMV-II cells also significantly decreased, while the expression of its mRNA was not affected. Melanin synthesis in a human skin model was also evaluated by the macro- and microscopic observation of its pigmentation as well as by quantitative measurements of melanin. Treatment of the human skin model with 250 μg of α-arbutin did not inhibit cell viability, while melanin synthesis was reduced to 40% of that in the control. These results indicate that α-arbutin is an effective and safe ingredient for skin-lightening. |
| doi_str_mv | 10.1248/bpb.27.510 |
| format | Article |
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Melanin synthesis in cells treated with α-arbutin at 0.5 mM decreased to 76% of that in non-treated cells. The cellular tyrosinase activity of HMV-II cells also significantly decreased, while the expression of its mRNA was not affected. Melanin synthesis in a human skin model was also evaluated by the macro- and microscopic observation of its pigmentation as well as by quantitative measurements of melanin. Treatment of the human skin model with 250 μg of α-arbutin did not inhibit cell viability, while melanin synthesis was reduced to 40% of that in the control. These results indicate that α-arbutin is an effective and safe ingredient for skin-lightening.</description><identifier>ISSN: 0918-6158</identifier><identifier>EISSN: 1347-5215</identifier><identifier>DOI: 10.1248/bpb.27.510</identifier><identifier>PMID: 15056856</identifier><language>eng</language><publisher>Japan: The Pharmaceutical Society of Japan</publisher><subject>Arbutin - pharmacology ; Cell Division - drug effects ; Enzyme Inhibitors - pharmacology ; human melanoma cell ; human tyrosinase ; Humans ; inhibition ; melanin ; Melanins - antagonists & inhibitors ; Melanins - biosynthesis ; Melanoma ; Models, Biological ; Monophenol Monooxygenase - antagonists & inhibitors ; Monophenol Monooxygenase - biosynthesis ; Monophenol Monooxygenase - genetics ; Organ Culture Techniques ; Reverse Transcriptase Polymerase Chain Reaction ; Skin - drug effects ; Skin - metabolism ; three-dimensional human skin model ; Tumor Cells, Cultured ; α-arbutin</subject><ispartof>Biological and Pharmaceutical Bulletin, 2004, Vol.27(4), pp.510-514</ispartof><rights>2004 The Pharmaceutical Society of Japan</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c502t-85cd78e7871d40cb16b8179d0cf5b1e5f862f900adf6afeafc1e68e222116f593</citedby><cites>FETCH-LOGICAL-c502t-85cd78e7871d40cb16b8179d0cf5b1e5f862f900adf6afeafc1e68e222116f593</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,1882,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15056856$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sugimoto, Kazuhisa</creatorcontrib><creatorcontrib>Nishimura, Takahisa</creatorcontrib><creatorcontrib>Nomura, Koji</creatorcontrib><creatorcontrib>Sugimoto, Kenji</creatorcontrib><creatorcontrib>Kuriki, Takashi</creatorcontrib><title>Inhibitory Effects of α-Arbutin on Melanin Synthesis in Cultured Human Melanoma Cells and a Three-Dimensional Human Skin Model</title><title>Biological & pharmaceutical bulletin</title><addtitle>Biol Pharm Bull</addtitle><description>We studied the inhibitory effects of 4-hydroxyphenyl α-glucopyranoside (α-arbutin) on melanogenesis in cultured human melanoma cells, HMV-II, and in a three-dimensional cultured human skin model. α-Arbutin showed no inhibitory effect on HMV-II cell growth at a concentration below 1.0 mM. Melanin synthesis in cells treated with α-arbutin at 0.5 mM decreased to 76% of that in non-treated cells. The cellular tyrosinase activity of HMV-II cells also significantly decreased, while the expression of its mRNA was not affected. Melanin synthesis in a human skin model was also evaluated by the macro- and microscopic observation of its pigmentation as well as by quantitative measurements of melanin. Treatment of the human skin model with 250 μg of α-arbutin did not inhibit cell viability, while melanin synthesis was reduced to 40% of that in the control. These results indicate that α-arbutin is an effective and safe ingredient for skin-lightening.</description><subject>Arbutin - pharmacology</subject><subject>Cell Division - drug effects</subject><subject>Enzyme Inhibitors - pharmacology</subject><subject>human melanoma cell</subject><subject>human tyrosinase</subject><subject>Humans</subject><subject>inhibition</subject><subject>melanin</subject><subject>Melanins - antagonists & inhibitors</subject><subject>Melanins - biosynthesis</subject><subject>Melanoma</subject><subject>Models, Biological</subject><subject>Monophenol Monooxygenase - antagonists & inhibitors</subject><subject>Monophenol Monooxygenase - biosynthesis</subject><subject>Monophenol Monooxygenase - genetics</subject><subject>Organ Culture Techniques</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Skin - drug effects</subject><subject>Skin - metabolism</subject><subject>three-dimensional human skin model</subject><subject>Tumor Cells, Cultured</subject><subject>α-arbutin</subject><issn>0918-6158</issn><issn>1347-5215</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkMtO3DAUhq2Kqgy0mz5A5RWLSpnaThx7VggNVwnUBXRtOc5xxzSxB9tZzKrP1BfhmWo0ATbnovPp09GP0FdKlpQ18ke37ZZMLDklH9CC1o2oOKP8AC3IisqqpVweoqOUHgkhgrD6EzqknPBW8naB_t74jetcDnGHL6wFkxMOFj__q85iN2XncfD4Dgbty3i_83kDySVclvU05ClCj6-nUc9MGDVewzAkrH2PNX7YRIDq3I3gkwteDzN8_6cI7kIPw2f00eohwZe5H6NflxcP6-vq9ufVzfrstjKcsFxJbnohQUhB-4aYjradpGLVE2N5R4Fb2TK7IkT3ttUWtDUUWgmMMUpby1f1MTrZe7cxPE2QshpdMuVV7SFMSYliq5v6Bfy-B00MKUWwahvdqONOUaJe4lYlbsWEKnEX-NtsnboR-nd0zrcAp3vgMWX9G94AHbMzA7y6mn0pyreL2eiowNf_AYoKk7g</recordid><startdate>20040401</startdate><enddate>20040401</enddate><creator>Sugimoto, Kazuhisa</creator><creator>Nishimura, Takahisa</creator><creator>Nomura, Koji</creator><creator>Sugimoto, Kenji</creator><creator>Kuriki, Takashi</creator><general>The Pharmaceutical Society of Japan</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20040401</creationdate><title>Inhibitory Effects of α-Arbutin on Melanin Synthesis in Cultured Human Melanoma Cells and a Three-Dimensional Human Skin Model</title><author>Sugimoto, Kazuhisa ; Nishimura, Takahisa ; Nomura, Koji ; Sugimoto, Kenji ; Kuriki, Takashi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c502t-85cd78e7871d40cb16b8179d0cf5b1e5f862f900adf6afeafc1e68e222116f593</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Arbutin - pharmacology</topic><topic>Cell Division - drug effects</topic><topic>Enzyme Inhibitors - pharmacology</topic><topic>human melanoma cell</topic><topic>human tyrosinase</topic><topic>Humans</topic><topic>inhibition</topic><topic>melanin</topic><topic>Melanins - antagonists & inhibitors</topic><topic>Melanins - biosynthesis</topic><topic>Melanoma</topic><topic>Models, Biological</topic><topic>Monophenol Monooxygenase - antagonists & inhibitors</topic><topic>Monophenol Monooxygenase - biosynthesis</topic><topic>Monophenol Monooxygenase - genetics</topic><topic>Organ Culture Techniques</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Skin - drug effects</topic><topic>Skin - metabolism</topic><topic>three-dimensional human skin model</topic><topic>Tumor Cells, Cultured</topic><topic>α-arbutin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sugimoto, Kazuhisa</creatorcontrib><creatorcontrib>Nishimura, Takahisa</creatorcontrib><creatorcontrib>Nomura, Koji</creatorcontrib><creatorcontrib>Sugimoto, Kenji</creatorcontrib><creatorcontrib>Kuriki, Takashi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biological & pharmaceutical bulletin</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sugimoto, Kazuhisa</au><au>Nishimura, Takahisa</au><au>Nomura, Koji</au><au>Sugimoto, Kenji</au><au>Kuriki, Takashi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inhibitory Effects of α-Arbutin on Melanin Synthesis in Cultured Human Melanoma Cells and a Three-Dimensional Human Skin Model</atitle><jtitle>Biological & pharmaceutical bulletin</jtitle><addtitle>Biol Pharm Bull</addtitle><date>2004-04-01</date><risdate>2004</risdate><volume>27</volume><issue>4</issue><spage>510</spage><epage>514</epage><pages>510-514</pages><issn>0918-6158</issn><eissn>1347-5215</eissn><abstract>We studied the inhibitory effects of 4-hydroxyphenyl α-glucopyranoside (α-arbutin) on melanogenesis in cultured human melanoma cells, HMV-II, and in a three-dimensional cultured human skin model. α-Arbutin showed no inhibitory effect on HMV-II cell growth at a concentration below 1.0 mM. Melanin synthesis in cells treated with α-arbutin at 0.5 mM decreased to 76% of that in non-treated cells. The cellular tyrosinase activity of HMV-II cells also significantly decreased, while the expression of its mRNA was not affected. Melanin synthesis in a human skin model was also evaluated by the macro- and microscopic observation of its pigmentation as well as by quantitative measurements of melanin. Treatment of the human skin model with 250 μg of α-arbutin did not inhibit cell viability, while melanin synthesis was reduced to 40% of that in the control. These results indicate that α-arbutin is an effective and safe ingredient for skin-lightening.</abstract><cop>Japan</cop><pub>The Pharmaceutical Society of Japan</pub><pmid>15056856</pmid><doi>10.1248/bpb.27.510</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Arbutin - pharmacology Cell Division - drug effects Enzyme Inhibitors - pharmacology human melanoma cell human tyrosinase Humans inhibition melanin Melanins - antagonists & inhibitors Melanins - biosynthesis Melanoma Models, Biological Monophenol Monooxygenase - antagonists & inhibitors Monophenol Monooxygenase - biosynthesis Monophenol Monooxygenase - genetics Organ Culture Techniques Reverse Transcriptase Polymerase Chain Reaction Skin - drug effects Skin - metabolism three-dimensional human skin model Tumor Cells, Cultured α-arbutin |
| title | Inhibitory Effects of α-Arbutin on Melanin Synthesis in Cultured Human Melanoma Cells and a Three-Dimensional Human Skin Model |
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