Development of a nested PCR assay to detect the pathogenic free-living amoeba Naegleria fowleri

Naegleria fowleri is the causative agent of primary amoebic meningoencephalitis, a fatal disease of the central nervous system that is acquired while swimming or diving in freshwater. A cDNA clone designated Mp2C15 obtained from N. fowleri was used as a probe to distinguish N. fowleri from other fre...

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Veröffentlicht in:	Parasitology research (1987) 2002-05, Vol.88 (5), p.443-450
Hauptverfasser:	Réveiller, Fabienne L, Cabanes, Pierre-André, Marciano-Cabral, Francine
Format:	Artikel
Sprache:	eng
Schlagworte:	Amebiasis - parasitology Animals DNA Probes DNA, Complementary DNA, Protozoan - analysis Fresh Water - parasitology Humans Molecular Sequence Data Naegleria fowleri - classification Naegleria fowleri - genetics Naegleria fowleri - isolation & purification Naegleria fowleri - pathogenicity Polymerase Chain Reaction - methods Sensitivity and Specificity Time Factors
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