Molecular and functional observations on the donor intestinal muscularis during human small bowel transplantation
Background & Aims: Ischemia-reperfusion injury or intestinal manipulation evokes an inflammatory response within the intestinal muscularis that is associated with intestinal dysmotility. We hypothesize that human small intestinal transplantation induces an analogous response. Methods: Human inte...
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| Veröffentlicht in: | Gastroenterology (New York, N.Y. 1943) N.Y. 1943), 2002-06, Vol.122 (7), p.1886-1897 |
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| container_title | Gastroenterology (New York, N.Y. 1943) |
| container_volume | 122 |
| creator | Türler, Andreas Kalff, Jörg C. Heeckt, Peter Abu–Elmagd, Kareem M. Schraut, Wolfgang H. Bond, Geoffrey J. Moore, Beverley A. Brünagel, Gisela Bauer, Anthony J. |
| description | Background & Aims: Ischemia-reperfusion injury or intestinal manipulation evokes an inflammatory response within the intestinal muscularis that is associated with intestinal dysmotility. We hypothesize that human small intestinal transplantation induces an analogous response. Methods: Human intestinal graft specimens were obtained during transplantation and compared with specimens removed early during elective bowel resections. Inflammatory gene expression was quantified by real-time reverse-transcription polymerase chain reaction. Histochemistry and immunohistochemistry were used to characterize leukocyte infiltration and macrophage activation. In vitro circular muscle contractility and intracellular electric neuromuscular transmission in response to electric field stimulation (EFS) were measured. Results: Messenger RNA (mRNA) values were significantly elevated before reperfusion and further increased during reperfusion (4 hour reperfusion: interleukin [IL]-6, 311-fold; monocyte chemoattractant protein [MCP-1, 122-fold; IL-8, 338-fold; epithelial neutrophil-activating peptide-78 [ENA-78], 56-fold; intercellular adhesion molecule-1 [ICAM-1], 9-fold; and cyclooxygenase-2 [COX2], 37-fold) over elective specimens. Neutrophils and monocytes extravasated in increased numbers in whole mounts before and after reperfusion over the elective specimens. Activated resident macrophages were identified as a major source of inflammatory mediators. Muscle contractions and neuromuscular transmission were markedly attenuated in the grafts. Conclusions: The data suggest that manipulation during organ harvesting initiates a functionally relevant molecular and cellular inflammatory response within the graft muscularis that is potentiated during the reperfusion period. Significant mechanical and neuromuscular functional alterations occurred during the transplant process.
GASTROENTEROLOGY 2002;122:1886-1897 |
| doi_str_mv | 10.1053/gast.2002.33628 |
| format | Article |
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GASTROENTEROLOGY 2002;122:1886-1897</description><identifier>ISSN: 0016-5085</identifier><identifier>EISSN: 1528-0012</identifier><identifier>DOI: 10.1053/gast.2002.33628</identifier><identifier>PMID: 12055596</identifier><identifier>CODEN: GASTAB</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Adult ; Biological and medical sciences ; Cytokines - genetics ; Digestive system ; Enteritis - physiopathology ; Gastrointestinal Motility ; Gene Expression ; Humans ; Inflammation Mediators - physiology ; Intestine, Small - cytology ; Intestine, Small - innervation ; Intestine, Small - physiopathology ; Intestine, Small - transplantation ; Investigative techniques, diagnostic techniques (general aspects) ; Leukocytes - pathology ; Macrophage Activation - physiology ; Medical sciences ; Muscle, Smooth - physiopathology ; Neuromuscular Junction - physiopathology ; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques ; Synaptic Transmission ; Tissue Donors</subject><ispartof>Gastroenterology (New York, N.Y. 1943), 2002-06, Vol.122 (7), p.1886-1897</ispartof><rights>2002 American Gastroenterological Association</rights><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c414t-cc917394e5d1f81356290da9b0d5690feb643b60103f0c32e926e060e3b30afd3</citedby><cites>FETCH-LOGICAL-c414t-cc917394e5d1f81356290da9b0d5690feb643b60103f0c32e926e060e3b30afd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0016508502000240$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13713339$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12055596$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Türler, Andreas</creatorcontrib><creatorcontrib>Kalff, Jörg C.</creatorcontrib><creatorcontrib>Heeckt, Peter</creatorcontrib><creatorcontrib>Abu–Elmagd, Kareem M.</creatorcontrib><creatorcontrib>Schraut, Wolfgang H.</creatorcontrib><creatorcontrib>Bond, Geoffrey J.</creatorcontrib><creatorcontrib>Moore, Beverley A.</creatorcontrib><creatorcontrib>Brünagel, Gisela</creatorcontrib><creatorcontrib>Bauer, Anthony J.</creatorcontrib><title>Molecular and functional observations on the donor intestinal muscularis during human small bowel transplantation</title><title>Gastroenterology (New York, N.Y. 1943)</title><addtitle>Gastroenterology</addtitle><description>Background & Aims: Ischemia-reperfusion injury or intestinal manipulation evokes an inflammatory response within the intestinal muscularis that is associated with intestinal dysmotility. We hypothesize that human small intestinal transplantation induces an analogous response. Methods: Human intestinal graft specimens were obtained during transplantation and compared with specimens removed early during elective bowel resections. Inflammatory gene expression was quantified by real-time reverse-transcription polymerase chain reaction. Histochemistry and immunohistochemistry were used to characterize leukocyte infiltration and macrophage activation. In vitro circular muscle contractility and intracellular electric neuromuscular transmission in response to electric field stimulation (EFS) were measured. Results: Messenger RNA (mRNA) values were significantly elevated before reperfusion and further increased during reperfusion (4 hour reperfusion: interleukin [IL]-6, 311-fold; monocyte chemoattractant protein [MCP-1, 122-fold; IL-8, 338-fold; epithelial neutrophil-activating peptide-78 [ENA-78], 56-fold; intercellular adhesion molecule-1 [ICAM-1], 9-fold; and cyclooxygenase-2 [COX2], 37-fold) over elective specimens. Neutrophils and monocytes extravasated in increased numbers in whole mounts before and after reperfusion over the elective specimens. Activated resident macrophages were identified as a major source of inflammatory mediators. Muscle contractions and neuromuscular transmission were markedly attenuated in the grafts. Conclusions: The data suggest that manipulation during organ harvesting initiates a functionally relevant molecular and cellular inflammatory response within the graft muscularis that is potentiated during the reperfusion period. Significant mechanical and neuromuscular functional alterations occurred during the transplant process.
GASTROENTEROLOGY 2002;122:1886-1897</description><subject>Adult</subject><subject>Biological and medical sciences</subject><subject>Cytokines - genetics</subject><subject>Digestive system</subject><subject>Enteritis - physiopathology</subject><subject>Gastrointestinal Motility</subject><subject>Gene Expression</subject><subject>Humans</subject><subject>Inflammation Mediators - physiology</subject><subject>Intestine, Small - cytology</subject><subject>Intestine, Small - innervation</subject><subject>Intestine, Small - physiopathology</subject><subject>Intestine, Small - transplantation</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Leukocytes - pathology</subject><subject>Macrophage Activation - physiology</subject><subject>Medical sciences</subject><subject>Muscle, Smooth - physiopathology</subject><subject>Neuromuscular Junction - physiopathology</subject><subject>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</subject><subject>Synaptic Transmission</subject><subject>Tissue Donors</subject><issn>0016-5085</issn><issn>1528-0012</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kEFv1DAQhS1ERZfCmRvyhd6yHcfrJD6iCgpSUS9wthx70ho59tZ2WvHvcXZX6qmn0Ujfe3r6CPnEYMtA8Kt7ncu2BWi3nHft8IZsmGiHBoC1b8mmnq4RMIhz8j7nvwAg-cDekXPWghBCdhvy-Ct6NIvXiepg6bQEU1wM2tM4ZkxPev0yjYGWB6Q2hpioCwVzcSs0L_kQdpnaJblwTx-WWQeaZ-09HeMzelqSDnnvdSiHsg_kbNI-48fTvSB_vn_7ff2jub27-Xn99bYxO7YrjTGS9VzuUFg2DYyLrpVgtRzBik7ChGO342MHDPgEhrco2w6hA-QjBz1ZfkEuj737FB-XOljNLhv0dQjGJaue9RJ6wSt4dQRNijknnNQ-uVmnf4qBWi2r1bJaLauD5Zr4fKpexhntC3_SWoEvJ0Bno_1UDRiXXzjeM865rJw8clhFPDlMKhuHwaB1CU1RNrpXR_wHVf6bZA</recordid><startdate>20020601</startdate><enddate>20020601</enddate><creator>Türler, Andreas</creator><creator>Kalff, Jörg C.</creator><creator>Heeckt, Peter</creator><creator>Abu–Elmagd, Kareem M.</creator><creator>Schraut, Wolfgang H.</creator><creator>Bond, Geoffrey J.</creator><creator>Moore, Beverley A.</creator><creator>Brünagel, Gisela</creator><creator>Bauer, Anthony J.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20020601</creationdate><title>Molecular and functional observations on the donor intestinal muscularis during human small bowel transplantation</title><author>Türler, Andreas ; Kalff, Jörg C. ; Heeckt, Peter ; Abu–Elmagd, Kareem M. ; Schraut, Wolfgang H. ; Bond, Geoffrey J. ; Moore, Beverley A. ; Brünagel, Gisela ; Bauer, Anthony J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c414t-cc917394e5d1f81356290da9b0d5690feb643b60103f0c32e926e060e3b30afd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Adult</topic><topic>Biological and medical sciences</topic><topic>Cytokines - genetics</topic><topic>Digestive system</topic><topic>Enteritis - physiopathology</topic><topic>Gastrointestinal Motility</topic><topic>Gene Expression</topic><topic>Humans</topic><topic>Inflammation Mediators - physiology</topic><topic>Intestine, Small - cytology</topic><topic>Intestine, Small - innervation</topic><topic>Intestine, Small - physiopathology</topic><topic>Intestine, Small - transplantation</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>Leukocytes - pathology</topic><topic>Macrophage Activation - physiology</topic><topic>Medical sciences</topic><topic>Muscle, Smooth - physiopathology</topic><topic>Neuromuscular Junction - physiopathology</topic><topic>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</topic><topic>Synaptic Transmission</topic><topic>Tissue Donors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Türler, Andreas</creatorcontrib><creatorcontrib>Kalff, Jörg C.</creatorcontrib><creatorcontrib>Heeckt, Peter</creatorcontrib><creatorcontrib>Abu–Elmagd, Kareem M.</creatorcontrib><creatorcontrib>Schraut, Wolfgang H.</creatorcontrib><creatorcontrib>Bond, Geoffrey J.</creatorcontrib><creatorcontrib>Moore, Beverley A.</creatorcontrib><creatorcontrib>Brünagel, Gisela</creatorcontrib><creatorcontrib>Bauer, Anthony J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Gastroenterology (New York, N.Y. 1943)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Türler, Andreas</au><au>Kalff, Jörg C.</au><au>Heeckt, Peter</au><au>Abu–Elmagd, Kareem M.</au><au>Schraut, Wolfgang H.</au><au>Bond, Geoffrey J.</au><au>Moore, Beverley A.</au><au>Brünagel, Gisela</au><au>Bauer, Anthony J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular and functional observations on the donor intestinal muscularis during human small bowel transplantation</atitle><jtitle>Gastroenterology (New York, N.Y. 1943)</jtitle><addtitle>Gastroenterology</addtitle><date>2002-06-01</date><risdate>2002</risdate><volume>122</volume><issue>7</issue><spage>1886</spage><epage>1897</epage><pages>1886-1897</pages><issn>0016-5085</issn><eissn>1528-0012</eissn><coden>GASTAB</coden><abstract>Background & Aims: Ischemia-reperfusion injury or intestinal manipulation evokes an inflammatory response within the intestinal muscularis that is associated with intestinal dysmotility. We hypothesize that human small intestinal transplantation induces an analogous response. Methods: Human intestinal graft specimens were obtained during transplantation and compared with specimens removed early during elective bowel resections. Inflammatory gene expression was quantified by real-time reverse-transcription polymerase chain reaction. Histochemistry and immunohistochemistry were used to characterize leukocyte infiltration and macrophage activation. In vitro circular muscle contractility and intracellular electric neuromuscular transmission in response to electric field stimulation (EFS) were measured. Results: Messenger RNA (mRNA) values were significantly elevated before reperfusion and further increased during reperfusion (4 hour reperfusion: interleukin [IL]-6, 311-fold; monocyte chemoattractant protein [MCP-1, 122-fold; IL-8, 338-fold; epithelial neutrophil-activating peptide-78 [ENA-78], 56-fold; intercellular adhesion molecule-1 [ICAM-1], 9-fold; and cyclooxygenase-2 [COX2], 37-fold) over elective specimens. Neutrophils and monocytes extravasated in increased numbers in whole mounts before and after reperfusion over the elective specimens. Activated resident macrophages were identified as a major source of inflammatory mediators. Muscle contractions and neuromuscular transmission were markedly attenuated in the grafts. Conclusions: The data suggest that manipulation during organ harvesting initiates a functionally relevant molecular and cellular inflammatory response within the graft muscularis that is potentiated during the reperfusion period. Significant mechanical and neuromuscular functional alterations occurred during the transplant process.
GASTROENTEROLOGY 2002;122:1886-1897</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>12055596</pmid><doi>10.1053/gast.2002.33628</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adult Biological and medical sciences Cytokines - genetics Digestive system Enteritis - physiopathology Gastrointestinal Motility Gene Expression Humans Inflammation Mediators - physiology Intestine, Small - cytology Intestine, Small - innervation Intestine, Small - physiopathology Intestine, Small - transplantation Investigative techniques, diagnostic techniques (general aspects) Leukocytes - pathology Macrophage Activation - physiology Medical sciences Muscle, Smooth - physiopathology Neuromuscular Junction - physiopathology Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Synaptic Transmission Tissue Donors |
| title | Molecular and functional observations on the donor intestinal muscularis during human small bowel transplantation |
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