Identification by differential display of a chicken tolloid-related metalloprotease specifically expressed in the caudal notochord

While the ventralizing factor Sonic hedgehog is expressed in the entire notochord (Development 121 (1995) 2537) the latter displays distinct ventralizing activities along its rostrocaudal axis. Hence, in HH stage-10 chicken embryo, the caudal notochord exhibits floor plate inducing capacities lost b...

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Veröffentlicht in:	Mechanisms of development 2000-08, Vol.96 (1), p.101-105
Hauptverfasser:	Liaubet, Laurence, Bertrand, Nicolas, Medevielle, François, Pituello, Fabienne
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals Astacin Blotting, Northern Bone Morphogenetic Proteins Chick Embryo Chicken embryo Cloning, Molecular Differential display Dorso-ventral patterning Floor plate In Situ Hybridization Metalloendopeptidases - biosynthesis Metalloendopeptidases - chemistry Metalloendopeptidases - genetics Metalloproteases Molecular Sequence Data Nerve Tissue Proteins - biosynthesis Nerve Tissue Proteins - chemistry Nerve Tissue Proteins - genetics Neural tube Notochord Notochord - metabolism Protein Biosynthesis Protein Structure, Tertiary Proteins - genetics Reverse Transcriptase Polymerase Chain Reaction Sequence Homology, Amino Acid Somite Spinal cord Time Factors Tissue Distribution Tolloid-Like Metalloproteinases Tolloid-related metalloprotease
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Zusammenfassung:	While the ventralizing factor Sonic hedgehog is expressed in the entire notochord (Development 121 (1995) 2537) the latter displays distinct ventralizing activities along its rostrocaudal axis. Hence, in HH stage-10 chicken embryo, the caudal notochord exhibits floor plate inducing capacities lost by rostral regions (Development 117 (1993) 205). Therefore, we hypothesize that the caudal notochord produces some cofactors which may contribute to its ventralizing properties. In order to identify such molecules we applied the differential display strategy and isolated a secreted Tolloid-related metalloprotease displaying a regionalized expression in the notochord.
ISSN:	0925-4773 1872-6356
DOI:	10.1016/S0925-4773(00)00382-8