Distribution of dynamins in testis and their possible relation to spermatogenesis
Dynamin 2 and dynamin 3 are highly expressed in testis. However, their functions in the tissue remain unclear. Considering that dynamin 1, neuron-specific isoform of dynamin, plays a pivotal role in endocytosis, functions of dynamin 2 and dynamin 3 in testis must be essential. Cellular expression an...
Gespeichert in:
| Veröffentlicht in: | Biochemical and biophysical research communications 2002-06, Vol.294 (2), p.261-267 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 267 |
|---|---|
| container_issue | 2 |
| container_start_page | 261 |
| container_title | Biochemical and biophysical research communications |
| container_volume | 294 |
| creator | Kamitani, Akihiro Yamada, Hiroshi Kinuta, Masahiro Watanabe, Masami Li, Shun-Ai Matsukawa, Takashi McNiven, Mark Kumon, Hiromi Takei, Kohji |
| description | Dynamin 2 and dynamin 3 are highly expressed in testis. However, their functions in the tissue remain unclear. Considering that dynamin 1, neuron-specific isoform of dynamin, plays a pivotal role in endocytosis, functions of dynamin 2 and dynamin 3 in testis must be essential. Cellular expression and subcellular localization of dynamin 2 and dynamin 3 in testis were investigated. Dynamin 2 and dynamin 3 were highly expressed in germ cells and Sertoli cells, constituents of seminiferous tubules. By immunofluorescence it was revealed that dynamin 2 colocalizes with clathrin both at the plasmamembrane and at Golgi in a cell line of Sertoli cells. Immunoreactivity for dynamin 3, on the other hand, appeared as finer puncta, which did not colocalize with clathrin, suggesting that these two dynamins have distinct functions in Sertoli cells. In the
klotho deficient mouse testis, which demonstrates disorder in spermatogenesis, expression of dynamin 2 and dynamin 3 was drastically reduced indicating possible association of these proteins with spermatogenesis. |
| doi_str_mv | 10.1016/S0006-291X(02)00470-9 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_71782817</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0006291X02004709</els_id><sourcerecordid>71782817</sourcerecordid><originalsourceid>FETCH-LOGICAL-c361t-8d5d8388324159e9d619d048725b3bdf59229eedb4e45693bbed40a8c587d8cf3</originalsourceid><addsrcrecordid>eNqFkMtKxTAQhoMonuPlEZSsRBfVSZq2yUrkeIUDIiq4C20z1UhvJqlw3t6eC7p0NZvvn3_mI-SIwTkDll48A0AaccXeToGfAYgMIrVFpgwURJyB2CbTX2RC9rz_BGBMpGqXTBiHhGUgpuTp2vrgbDEE27W0q6hZtHljW09tSwP6YD3NW0PDB1pH-857W9RIHdb5KhE66nt0TR66d2zRW39Adqq89ni4mfvk9fbmZXYfzR_vHmZX86iMUxYiaRIjYyljLliiUJmUKQNCZjwp4sJUieJcIZpCoEhSFRcFGgG5LBOZGVlW8T45We_tXfc1jJfqxvoS6zpvsRu8zlgmuWTZCCZrsHTj-Q4r3Tvb5G6hGeilS71yqZeiNHC9cqnVmDveFAxFg-YvtZE3ApdrAMc3vy067UuLbYnGOiyDNp39p-IHWcSEzg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>71782817</pqid></control><display><type>article</type><title>Distribution of dynamins in testis and their possible relation to spermatogenesis</title><source>MEDLINE</source><source>Access via ScienceDirect (Elsevier)</source><creator>Kamitani, Akihiro ; Yamada, Hiroshi ; Kinuta, Masahiro ; Watanabe, Masami ; Li, Shun-Ai ; Matsukawa, Takashi ; McNiven, Mark ; Kumon, Hiromi ; Takei, Kohji</creator><creatorcontrib>Kamitani, Akihiro ; Yamada, Hiroshi ; Kinuta, Masahiro ; Watanabe, Masami ; Li, Shun-Ai ; Matsukawa, Takashi ; McNiven, Mark ; Kumon, Hiromi ; Takei, Kohji</creatorcontrib><description>Dynamin 2 and dynamin 3 are highly expressed in testis. However, their functions in the tissue remain unclear. Considering that dynamin 1, neuron-specific isoform of dynamin, plays a pivotal role in endocytosis, functions of dynamin 2 and dynamin 3 in testis must be essential. Cellular expression and subcellular localization of dynamin 2 and dynamin 3 in testis were investigated. Dynamin 2 and dynamin 3 were highly expressed in germ cells and Sertoli cells, constituents of seminiferous tubules. By immunofluorescence it was revealed that dynamin 2 colocalizes with clathrin both at the plasmamembrane and at Golgi in a cell line of Sertoli cells. Immunoreactivity for dynamin 3, on the other hand, appeared as finer puncta, which did not colocalize with clathrin, suggesting that these two dynamins have distinct functions in Sertoli cells. In the
klotho deficient mouse testis, which demonstrates disorder in spermatogenesis, expression of dynamin 2 and dynamin 3 was drastically reduced indicating possible association of these proteins with spermatogenesis.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/S0006-291X(02)00470-9</identifier><identifier>PMID: 12051704</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Autoantigens ; Blotting, Western ; Cell Count ; Cell Membrane - metabolism ; Cells, Cultured ; Clathrin - biosynthesis ; Dynamin ; Dynamin I ; Dynamins ; Fluorescent Antibody Technique ; Germ Cells - cytology ; Germ Cells - metabolism ; Glucuronidase ; Golgi apparatus ; Golgi Apparatus - metabolism ; GTP Phosphohydrolases - biosynthesis ; klotho mouse ; Male ; Membrane Proteins - biosynthesis ; Membrane Proteins - deficiency ; Mice ; Mice, Mutant Strains ; Nerve Tissue Proteins - biosynthesis ; Phosphoric Monoester Hydrolases - biosynthesis ; Rats ; Rats, Wistar ; Sertoli Cells - cytology ; Sertoli Cells - metabolism ; Spermatogenesis ; Spermatogenesis - physiology ; Testis ; Testis - cytology ; Testis - metabolism ; Vesicle formation</subject><ispartof>Biochemical and biophysical research communications, 2002-06, Vol.294 (2), p.261-267</ispartof><rights>2002 Elsevier Science (USA)</rights><rights>(c) 2002 Elsevier Science (USA).</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c361t-8d5d8388324159e9d619d048725b3bdf59229eedb4e45693bbed40a8c587d8cf3</citedby><cites>FETCH-LOGICAL-c361t-8d5d8388324159e9d619d048725b3bdf59229eedb4e45693bbed40a8c587d8cf3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0006-291X(02)00470-9$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12051704$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kamitani, Akihiro</creatorcontrib><creatorcontrib>Yamada, Hiroshi</creatorcontrib><creatorcontrib>Kinuta, Masahiro</creatorcontrib><creatorcontrib>Watanabe, Masami</creatorcontrib><creatorcontrib>Li, Shun-Ai</creatorcontrib><creatorcontrib>Matsukawa, Takashi</creatorcontrib><creatorcontrib>McNiven, Mark</creatorcontrib><creatorcontrib>Kumon, Hiromi</creatorcontrib><creatorcontrib>Takei, Kohji</creatorcontrib><title>Distribution of dynamins in testis and their possible relation to spermatogenesis</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Dynamin 2 and dynamin 3 are highly expressed in testis. However, their functions in the tissue remain unclear. Considering that dynamin 1, neuron-specific isoform of dynamin, plays a pivotal role in endocytosis, functions of dynamin 2 and dynamin 3 in testis must be essential. Cellular expression and subcellular localization of dynamin 2 and dynamin 3 in testis were investigated. Dynamin 2 and dynamin 3 were highly expressed in germ cells and Sertoli cells, constituents of seminiferous tubules. By immunofluorescence it was revealed that dynamin 2 colocalizes with clathrin both at the plasmamembrane and at Golgi in a cell line of Sertoli cells. Immunoreactivity for dynamin 3, on the other hand, appeared as finer puncta, which did not colocalize with clathrin, suggesting that these two dynamins have distinct functions in Sertoli cells. In the
klotho deficient mouse testis, which demonstrates disorder in spermatogenesis, expression of dynamin 2 and dynamin 3 was drastically reduced indicating possible association of these proteins with spermatogenesis.</description><subject>Animals</subject><subject>Autoantigens</subject><subject>Blotting, Western</subject><subject>Cell Count</subject><subject>Cell Membrane - metabolism</subject><subject>Cells, Cultured</subject><subject>Clathrin - biosynthesis</subject><subject>Dynamin</subject><subject>Dynamin I</subject><subject>Dynamins</subject><subject>Fluorescent Antibody Technique</subject><subject>Germ Cells - cytology</subject><subject>Germ Cells - metabolism</subject><subject>Glucuronidase</subject><subject>Golgi apparatus</subject><subject>Golgi Apparatus - metabolism</subject><subject>GTP Phosphohydrolases - biosynthesis</subject><subject>klotho mouse</subject><subject>Male</subject><subject>Membrane Proteins - biosynthesis</subject><subject>Membrane Proteins - deficiency</subject><subject>Mice</subject><subject>Mice, Mutant Strains</subject><subject>Nerve Tissue Proteins - biosynthesis</subject><subject>Phosphoric Monoester Hydrolases - biosynthesis</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Sertoli Cells - cytology</subject><subject>Sertoli Cells - metabolism</subject><subject>Spermatogenesis</subject><subject>Spermatogenesis - physiology</subject><subject>Testis</subject><subject>Testis - cytology</subject><subject>Testis - metabolism</subject><subject>Vesicle formation</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMtKxTAQhoMonuPlEZSsRBfVSZq2yUrkeIUDIiq4C20z1UhvJqlw3t6eC7p0NZvvn3_mI-SIwTkDll48A0AaccXeToGfAYgMIrVFpgwURJyB2CbTX2RC9rz_BGBMpGqXTBiHhGUgpuTp2vrgbDEE27W0q6hZtHljW09tSwP6YD3NW0PDB1pH-857W9RIHdb5KhE66nt0TR66d2zRW39Adqq89ni4mfvk9fbmZXYfzR_vHmZX86iMUxYiaRIjYyljLliiUJmUKQNCZjwp4sJUieJcIZpCoEhSFRcFGgG5LBOZGVlW8T45We_tXfc1jJfqxvoS6zpvsRu8zlgmuWTZCCZrsHTj-Q4r3Tvb5G6hGeilS71yqZeiNHC9cqnVmDveFAxFg-YvtZE3ApdrAMc3vy067UuLbYnGOiyDNp39p-IHWcSEzg</recordid><startdate>20020607</startdate><enddate>20020607</enddate><creator>Kamitani, Akihiro</creator><creator>Yamada, Hiroshi</creator><creator>Kinuta, Masahiro</creator><creator>Watanabe, Masami</creator><creator>Li, Shun-Ai</creator><creator>Matsukawa, Takashi</creator><creator>McNiven, Mark</creator><creator>Kumon, Hiromi</creator><creator>Takei, Kohji</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20020607</creationdate><title>Distribution of dynamins in testis and their possible relation to spermatogenesis</title><author>Kamitani, Akihiro ; Yamada, Hiroshi ; Kinuta, Masahiro ; Watanabe, Masami ; Li, Shun-Ai ; Matsukawa, Takashi ; McNiven, Mark ; Kumon, Hiromi ; Takei, Kohji</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c361t-8d5d8388324159e9d619d048725b3bdf59229eedb4e45693bbed40a8c587d8cf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Animals</topic><topic>Autoantigens</topic><topic>Blotting, Western</topic><topic>Cell Count</topic><topic>Cell Membrane - metabolism</topic><topic>Cells, Cultured</topic><topic>Clathrin - biosynthesis</topic><topic>Dynamin</topic><topic>Dynamin I</topic><topic>Dynamins</topic><topic>Fluorescent Antibody Technique</topic><topic>Germ Cells - cytology</topic><topic>Germ Cells - metabolism</topic><topic>Glucuronidase</topic><topic>Golgi apparatus</topic><topic>Golgi Apparatus - metabolism</topic><topic>GTP Phosphohydrolases - biosynthesis</topic><topic>klotho mouse</topic><topic>Male</topic><topic>Membrane Proteins - biosynthesis</topic><topic>Membrane Proteins - deficiency</topic><topic>Mice</topic><topic>Mice, Mutant Strains</topic><topic>Nerve Tissue Proteins - biosynthesis</topic><topic>Phosphoric Monoester Hydrolases - biosynthesis</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Sertoli Cells - cytology</topic><topic>Sertoli Cells - metabolism</topic><topic>Spermatogenesis</topic><topic>Spermatogenesis - physiology</topic><topic>Testis</topic><topic>Testis - cytology</topic><topic>Testis - metabolism</topic><topic>Vesicle formation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kamitani, Akihiro</creatorcontrib><creatorcontrib>Yamada, Hiroshi</creatorcontrib><creatorcontrib>Kinuta, Masahiro</creatorcontrib><creatorcontrib>Watanabe, Masami</creatorcontrib><creatorcontrib>Li, Shun-Ai</creatorcontrib><creatorcontrib>Matsukawa, Takashi</creatorcontrib><creatorcontrib>McNiven, Mark</creatorcontrib><creatorcontrib>Kumon, Hiromi</creatorcontrib><creatorcontrib>Takei, Kohji</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kamitani, Akihiro</au><au>Yamada, Hiroshi</au><au>Kinuta, Masahiro</au><au>Watanabe, Masami</au><au>Li, Shun-Ai</au><au>Matsukawa, Takashi</au><au>McNiven, Mark</au><au>Kumon, Hiromi</au><au>Takei, Kohji</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Distribution of dynamins in testis and their possible relation to spermatogenesis</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2002-06-07</date><risdate>2002</risdate><volume>294</volume><issue>2</issue><spage>261</spage><epage>267</epage><pages>261-267</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>Dynamin 2 and dynamin 3 are highly expressed in testis. However, their functions in the tissue remain unclear. Considering that dynamin 1, neuron-specific isoform of dynamin, plays a pivotal role in endocytosis, functions of dynamin 2 and dynamin 3 in testis must be essential. Cellular expression and subcellular localization of dynamin 2 and dynamin 3 in testis were investigated. Dynamin 2 and dynamin 3 were highly expressed in germ cells and Sertoli cells, constituents of seminiferous tubules. By immunofluorescence it was revealed that dynamin 2 colocalizes with clathrin both at the plasmamembrane and at Golgi in a cell line of Sertoli cells. Immunoreactivity for dynamin 3, on the other hand, appeared as finer puncta, which did not colocalize with clathrin, suggesting that these two dynamins have distinct functions in Sertoli cells. In the
klotho deficient mouse testis, which demonstrates disorder in spermatogenesis, expression of dynamin 2 and dynamin 3 was drastically reduced indicating possible association of these proteins with spermatogenesis.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>12051704</pmid><doi>10.1016/S0006-291X(02)00470-9</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0006-291X |
| ispartof | Biochemical and biophysical research communications, 2002-06, Vol.294 (2), p.261-267 |
| issn | 0006-291X 1090-2104 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_71782817 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Animals Autoantigens Blotting, Western Cell Count Cell Membrane - metabolism Cells, Cultured Clathrin - biosynthesis Dynamin Dynamin I Dynamins Fluorescent Antibody Technique Germ Cells - cytology Germ Cells - metabolism Glucuronidase Golgi apparatus Golgi Apparatus - metabolism GTP Phosphohydrolases - biosynthesis klotho mouse Male Membrane Proteins - biosynthesis Membrane Proteins - deficiency Mice Mice, Mutant Strains Nerve Tissue Proteins - biosynthesis Phosphoric Monoester Hydrolases - biosynthesis Rats Rats, Wistar Sertoli Cells - cytology Sertoli Cells - metabolism Spermatogenesis Spermatogenesis - physiology Testis Testis - cytology Testis - metabolism Vesicle formation |
| title | Distribution of dynamins in testis and their possible relation to spermatogenesis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-20T20%3A21%3A41IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Distribution%20of%20dynamins%20in%20testis%20and%20their%20possible%20relation%20to%20spermatogenesis&rft.jtitle=Biochemical%20and%20biophysical%20research%20communications&rft.au=Kamitani,%20Akihiro&rft.date=2002-06-07&rft.volume=294&rft.issue=2&rft.spage=261&rft.epage=267&rft.pages=261-267&rft.issn=0006-291X&rft.eissn=1090-2104&rft_id=info:doi/10.1016/S0006-291X(02)00470-9&rft_dat=%3Cproquest_cross%3E71782817%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=71782817&rft_id=info:pmid/12051704&rft_els_id=S0006291X02004709&rfr_iscdi=true |