Two-compartment model for whole-cell data analysis and transient compensation
Recording and analysis of neuronal patch-clamp data involve many assumptions about membrane properties and cell morphology. Some of these assumptions introduce large errors or oversimplifications into the results. In particular, dendritic branching with high intracellular resistance leads to difficu...
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| Veröffentlicht in: | Journal of neuroscience methods 2000-06, Vol.99 (1), p.25-35 |
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| container_title | Journal of neuroscience methods |
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| creator | Nadeau, H. Lester, H.A. |
| description | Recording and analysis of neuronal patch-clamp data involve many assumptions about membrane properties and cell morphology. Some of these assumptions introduce large errors or oversimplifications into the results. In particular, dendritic branching with high intracellular resistance leads to difficulty with capacitance calculation and transient subtraction, and may significantly distort measured currents. A two-compartment model, presented in detail here, provides a simple method of reducing many of these problems for the relatively simple case of cultured neurons studied with whole-cell patch electrodes. Some passive membrane properties may be accurately calculated, and the results may be used to correct recorded currents for resulting series resistance, intracellular resistance, and capacitive transient errors. The model may be tailored to particular cell types or experimental conditions. Programs to implement the algorithms are available from http://www.its.caltech.edu/∼nadeau/Rscomp.html. |
| doi_str_mv | 10.1016/S0165-0270(00)00210-7 |
| format | Article |
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Some of these assumptions introduce large errors or oversimplifications into the results. In particular, dendritic branching with high intracellular resistance leads to difficulty with capacitance calculation and transient subtraction, and may significantly distort measured currents. A two-compartment model, presented in detail here, provides a simple method of reducing many of these problems for the relatively simple case of cultured neurons studied with whole-cell patch electrodes. Some passive membrane properties may be accurately calculated, and the results may be used to correct recorded currents for resulting series resistance, intracellular resistance, and capacitive transient errors. The model may be tailored to particular cell types or experimental conditions. 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Some of these assumptions introduce large errors or oversimplifications into the results. In particular, dendritic branching with high intracellular resistance leads to difficulty with capacitance calculation and transient subtraction, and may significantly distort measured currents. A two-compartment model, presented in detail here, provides a simple method of reducing many of these problems for the relatively simple case of cultured neurons studied with whole-cell patch electrodes. Some passive membrane properties may be accurately calculated, and the results may be used to correct recorded currents for resulting series resistance, intracellular resistance, and capacitive transient errors. The model may be tailored to particular cell types or experimental conditions. Programs to implement the algorithms are available from http://www.its.caltech.edu/∼nadeau/Rscomp.html.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cable</subject><subject>Capacitance</subject><subject>Cell Compartmentation - physiology</subject><subject>Cell Size - physiology</subject><subject>Cells, Cultured</subject><subject>Compartmental model</subject><subject>Compensation</subject><subject>Dendrites - physiology</subject><subject>Dendrites - ultrastructure</subject><subject>Female</subject><subject>Fetus</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects. Models. Methods</subject><subject>Membrane Potentials - physiology</subject><subject>Models, Neurological</subject><subject>Patch-Clamp Techniques</subject><subject>Pregnancy</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Series resistance</subject><subject>Vertebrates: nervous system and sense organs</subject><subject>Voltage-clamp</subject><subject>Whole-cell recording</subject><issn>0165-0270</issn><issn>1872-678X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1LxDAURYMoOn78BKULEV1UX5JOk65EBr9AcaGCu5CmrxhpmzHpKPPvTe2g7oRHXhbnJpdDyD6FUwo0P3uMxzQFJuAY4ASAUUjFGplQKViaC_myTiY_yBbZDuENALIC8k2yRaHgec6LCbl_-nSpce1c-77Frk9aV2GT1M4nn6-uwdRg0ySV7nWiO90sgw3xUiW9112wQ2AIYxd0b123SzZq3QTcW-0d8nx1-TS7Se8erm9nF3ep4QX0acaxhiyvWVGKKseCyaykHEzJMmQlF6XEvKQ0oyCllplgUDMqRFVPkXHGke-Qo_HduXfvCwy9am0YmuoO3SIoQYWkTEIEpyNovAvBY63m3rbaLxUFNXhU3x7VIEnBMNGjEjF3sPpgUbZY_UmN4iJwuAJ0MLqpow5jwy-XccEEjdj5iGG08WHRq2CiNYOV9Wh6VTn7T5MvufiOYg</recordid><startdate>20000630</startdate><enddate>20000630</enddate><creator>Nadeau, H.</creator><creator>Lester, H.A.</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20000630</creationdate><title>Two-compartment model for whole-cell data analysis and transient compensation</title><author>Nadeau, H. ; Lester, H.A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c390t-43ef046f29b7d6e9284b130cb24e2b37b8e6b1141088a84720f2177df5e2323e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cable</topic><topic>Capacitance</topic><topic>Cell Compartmentation - physiology</topic><topic>Cell Size - physiology</topic><topic>Cells, Cultured</topic><topic>Compartmental model</topic><topic>Compensation</topic><topic>Dendrites - physiology</topic><topic>Dendrites - ultrastructure</topic><topic>Female</topic><topic>Fetus</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects. Models. Methods</topic><topic>Membrane Potentials - physiology</topic><topic>Models, Neurological</topic><topic>Patch-Clamp Techniques</topic><topic>Pregnancy</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Series resistance</topic><topic>Vertebrates: nervous system and sense organs</topic><topic>Voltage-clamp</topic><topic>Whole-cell recording</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nadeau, H.</creatorcontrib><creatorcontrib>Lester, H.A.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of neuroscience methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nadeau, H.</au><au>Lester, H.A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Two-compartment model for whole-cell data analysis and transient compensation</atitle><jtitle>Journal of neuroscience methods</jtitle><addtitle>J Neurosci Methods</addtitle><date>2000-06-30</date><risdate>2000</risdate><volume>99</volume><issue>1</issue><spage>25</spage><epage>35</epage><pages>25-35</pages><issn>0165-0270</issn><eissn>1872-678X</eissn><coden>JNMEDT</coden><abstract>Recording and analysis of neuronal patch-clamp data involve many assumptions about membrane properties and cell morphology. Some of these assumptions introduce large errors or oversimplifications into the results. In particular, dendritic branching with high intracellular resistance leads to difficulty with capacitance calculation and transient subtraction, and may significantly distort measured currents. A two-compartment model, presented in detail here, provides a simple method of reducing many of these problems for the relatively simple case of cultured neurons studied with whole-cell patch electrodes. Some passive membrane properties may be accurately calculated, and the results may be used to correct recorded currents for resulting series resistance, intracellular resistance, and capacitive transient errors. The model may be tailored to particular cell types or experimental conditions. 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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals Biological and medical sciences Cable Capacitance Cell Compartmentation - physiology Cell Size - physiology Cells, Cultured Compartmental model Compensation Dendrites - physiology Dendrites - ultrastructure Female Fetus Fundamental and applied biological sciences. Psychology General aspects. Models. Methods Membrane Potentials - physiology Models, Neurological Patch-Clamp Techniques Pregnancy Rats Rats, Wistar Series resistance Vertebrates: nervous system and sense organs Voltage-clamp Whole-cell recording |
| title | Two-compartment model for whole-cell data analysis and transient compensation |
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