Transcription of purine transporter genes is activated during the isotropic growth phase of Aspergillus nidulans conidia
Summary Aspergillus nidulans possesses three well‐characterized purine transporters encoded by the genes uapA, uapC and azgA. Expression of these genes in mycelium is induced by purines and repressed by ammonium or glutamine through the action of the pathway‐specific UaY regulator and the general GA...
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| Veröffentlicht in: | Molecular microbiology 2004-04, Vol.52 (1), p.205-216 |
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| creator | Amillis, Sotiris Cecchetto, Gianna Sophianopoulou, Vicky Koukaki, Marina Scazzocchio, Claudio Diallinas, George |
| description | Summary
Aspergillus nidulans possesses three well‐characterized purine transporters encoded by the genes uapA, uapC and azgA. Expression of these genes in mycelium is induced by purines and repressed by ammonium or glutamine through the action of the pathway‐specific UaY regulator and the general GATA factor AreA respectively. Here, we describe the regulation of expression of purine transporters during conidiospore germination and the onset of mycelium development. In resting conidiospores, mRNA steady‐state levels of purine transporter genes and purine uptake activities are undetectable or very low. Both mRNA steady‐state levels and purine transport activities increase substantially during the isotropic growth phase of conidial germination. Both processes occur in the absence of purine induction and independently of the nitrogen source present in the medium. The transcriptional activator UaY is dispensable for the germination‐induced expression of the three transporter genes. AreA, on the other hand, is essential for the expression of uapA, but not for that of azgA or uapC, during germination. Transcriptional activation of uapA, uapC and azgA during germination is also independent of the presence of a carbon source in the medium. This work establishes the presence of a novel system triggering purine transporter transcription during germination. Similar results have been found in studies on the expression of other transporters in A. nidulans, suggesting that global expression of transporters might operate as a general system for sensing solute availability. |
| doi_str_mv | 10.1046/j.1365-2958.2003.03956.x |
| format | Article |
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Aspergillus nidulans possesses three well‐characterized purine transporters encoded by the genes uapA, uapC and azgA. Expression of these genes in mycelium is induced by purines and repressed by ammonium or glutamine through the action of the pathway‐specific UaY regulator and the general GATA factor AreA respectively. Here, we describe the regulation of expression of purine transporters during conidiospore germination and the onset of mycelium development. In resting conidiospores, mRNA steady‐state levels of purine transporter genes and purine uptake activities are undetectable or very low. Both mRNA steady‐state levels and purine transport activities increase substantially during the isotropic growth phase of conidial germination. Both processes occur in the absence of purine induction and independently of the nitrogen source present in the medium. The transcriptional activator UaY is dispensable for the germination‐induced expression of the three transporter genes. AreA, on the other hand, is essential for the expression of uapA, but not for that of azgA or uapC, during germination. Transcriptional activation of uapA, uapC and azgA during germination is also independent of the presence of a carbon source in the medium. This work establishes the presence of a novel system triggering purine transporter transcription during germination. Similar results have been found in studies on the expression of other transporters in A. nidulans, suggesting that global expression of transporters might operate as a general system for sensing solute availability.</description><identifier>ISSN: 0950-382X</identifier><identifier>EISSN: 1365-2958</identifier><identifier>DOI: 10.1046/j.1365-2958.2003.03956.x</identifier><identifier>PMID: 15049821</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Ltd</publisher><subject>Aspergillus nidulans - genetics ; Aspergillus nidulans - growth & development ; Aspergillus nidulans - physiology ; Biological and medical sciences ; Blotting, Northern ; Fundamental and applied biological sciences. Psychology ; Fungal Proteins - genetics ; Fungal Proteins - metabolism ; Fungal Proteins - physiology ; Gene Expression Regulation, Fungal ; Genes, Fungal ; Hypoxanthine - metabolism ; Membrane Transport Proteins - genetics ; Membrane Transport Proteins - metabolism ; Microbiology ; Miscellaneous ; Mycelium - growth & development ; Mycology ; Nucleobase Transport Proteins - genetics ; Nucleobase Transport Proteins - metabolism ; Purines - metabolism ; Spores, Fungal - genetics ; Spores, Fungal - metabolism ; Trans-Activators - genetics ; Trans-Activators - metabolism ; Transcription Factors - genetics ; Transcription Factors - metabolism ; Transcription, Genetic ; Transcriptional Activation ; Xanthine - metabolism</subject><ispartof>Molecular microbiology, 2004-04, Vol.52 (1), p.205-216</ispartof><rights>2004 INIST-CNRS</rights><rights>Copyright Blackwell Scientific Publications Ltd. Apr 2004</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4726-bfcf67e5cc557490977aba817f510db31a25d0489d68e61ffb83c3f4cf71e9163</citedby><cites>FETCH-LOGICAL-c4726-bfcf67e5cc557490977aba817f510db31a25d0489d68e61ffb83c3f4cf71e9163</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1365-2958.2003.03956.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.1365-2958.2003.03956.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,1432,27923,27924,45573,45574,46408,46832</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15592878$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15049821$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Amillis, Sotiris</creatorcontrib><creatorcontrib>Cecchetto, Gianna</creatorcontrib><creatorcontrib>Sophianopoulou, Vicky</creatorcontrib><creatorcontrib>Koukaki, Marina</creatorcontrib><creatorcontrib>Scazzocchio, Claudio</creatorcontrib><creatorcontrib>Diallinas, George</creatorcontrib><title>Transcription of purine transporter genes is activated during the isotropic growth phase of Aspergillus nidulans conidia</title><title>Molecular microbiology</title><addtitle>Mol Microbiol</addtitle><description>Summary
Aspergillus nidulans possesses three well‐characterized purine transporters encoded by the genes uapA, uapC and azgA. Expression of these genes in mycelium is induced by purines and repressed by ammonium or glutamine through the action of the pathway‐specific UaY regulator and the general GATA factor AreA respectively. Here, we describe the regulation of expression of purine transporters during conidiospore germination and the onset of mycelium development. In resting conidiospores, mRNA steady‐state levels of purine transporter genes and purine uptake activities are undetectable or very low. Both mRNA steady‐state levels and purine transport activities increase substantially during the isotropic growth phase of conidial germination. Both processes occur in the absence of purine induction and independently of the nitrogen source present in the medium. The transcriptional activator UaY is dispensable for the germination‐induced expression of the three transporter genes. AreA, on the other hand, is essential for the expression of uapA, but not for that of azgA or uapC, during germination. Transcriptional activation of uapA, uapC and azgA during germination is also independent of the presence of a carbon source in the medium. This work establishes the presence of a novel system triggering purine transporter transcription during germination. Similar results have been found in studies on the expression of other transporters in A. nidulans, suggesting that global expression of transporters might operate as a general system for sensing solute availability.</description><subject>Aspergillus nidulans - genetics</subject><subject>Aspergillus nidulans - growth & development</subject><subject>Aspergillus nidulans - physiology</subject><subject>Biological and medical sciences</subject><subject>Blotting, Northern</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fungal Proteins - genetics</subject><subject>Fungal Proteins - metabolism</subject><subject>Fungal Proteins - physiology</subject><subject>Gene Expression Regulation, Fungal</subject><subject>Genes, Fungal</subject><subject>Hypoxanthine - metabolism</subject><subject>Membrane Transport Proteins - genetics</subject><subject>Membrane Transport Proteins - metabolism</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Mycelium - growth & development</subject><subject>Mycology</subject><subject>Nucleobase Transport Proteins - genetics</subject><subject>Nucleobase Transport Proteins - metabolism</subject><subject>Purines - metabolism</subject><subject>Spores, Fungal - genetics</subject><subject>Spores, Fungal - metabolism</subject><subject>Trans-Activators - genetics</subject><subject>Trans-Activators - metabolism</subject><subject>Transcription Factors - genetics</subject><subject>Transcription Factors - metabolism</subject><subject>Transcription, Genetic</subject><subject>Transcriptional Activation</subject><subject>Xanthine - metabolism</subject><issn>0950-382X</issn><issn>1365-2958</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1q3DAUhUVpSKZpXqGIQruzK1nW36KLEPoTSOgmhe6ELEszGjyWK8nJ5O0rZ4Y2ZNWVLlffPRz4AIAY1Ri17NO2xoTRqpFU1A1CpEZEUlbvX4HV34_XYIUkRRURza8z8CalLUKYIEZOwRmmqJWiwSuwv4t6TCb6KfswwuDgNEc_WpiX_RRithGu7WgT9Alqk_29zraH_UKtYd7Ysg85hskbuI7hIW_gtNHJLlGXabJx7YdhTnD0_TyUSGhCGb1-C06cHpK9OL7n4OfXL3dX36ubH9-ury5vKtPyhlWdM45xS42hlLcSSc51pwXmjmLUdwTrhvaoFbJnwjLsXCeIIa41jmMrMSPn4OMhd4rh92xTVjufjB1KFxvmpDjmXHDeFvD9C3Ab5jiWbgpLRjGRDSqQOEAmhpSidWqKfqfjo8JILWrUVi0G1GJALWrUkxq1L6fvjvlzt7P9v8OjiwJ8OAI6GT24IsD49IyjshFcFO7zgXvwg3387wLq9vZ6mcgf1LSsWg</recordid><startdate>200404</startdate><enddate>200404</enddate><creator>Amillis, Sotiris</creator><creator>Cecchetto, Gianna</creator><creator>Sophianopoulou, Vicky</creator><creator>Koukaki, Marina</creator><creator>Scazzocchio, Claudio</creator><creator>Diallinas, George</creator><general>Blackwell Science Ltd</general><general>Blackwell Science</general><general>Blackwell Publishing Ltd</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>200404</creationdate><title>Transcription of purine transporter genes is activated during the isotropic growth phase of Aspergillus nidulans conidia</title><author>Amillis, Sotiris ; Cecchetto, Gianna ; Sophianopoulou, Vicky ; Koukaki, Marina ; Scazzocchio, Claudio ; Diallinas, George</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4726-bfcf67e5cc557490977aba817f510db31a25d0489d68e61ffb83c3f4cf71e9163</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Aspergillus nidulans - genetics</topic><topic>Aspergillus nidulans - growth & development</topic><topic>Aspergillus nidulans - physiology</topic><topic>Biological and medical sciences</topic><topic>Blotting, Northern</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fungal Proteins - genetics</topic><topic>Fungal Proteins - metabolism</topic><topic>Fungal Proteins - physiology</topic><topic>Gene Expression Regulation, Fungal</topic><topic>Genes, Fungal</topic><topic>Hypoxanthine - metabolism</topic><topic>Membrane Transport Proteins - genetics</topic><topic>Membrane Transport Proteins - metabolism</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Mycelium - growth & development</topic><topic>Mycology</topic><topic>Nucleobase Transport Proteins - genetics</topic><topic>Nucleobase Transport Proteins - metabolism</topic><topic>Purines - metabolism</topic><topic>Spores, Fungal - genetics</topic><topic>Spores, Fungal - metabolism</topic><topic>Trans-Activators - genetics</topic><topic>Trans-Activators - metabolism</topic><topic>Transcription Factors - genetics</topic><topic>Transcription Factors - metabolism</topic><topic>Transcription, Genetic</topic><topic>Transcriptional Activation</topic><topic>Xanthine - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Amillis, Sotiris</creatorcontrib><creatorcontrib>Cecchetto, Gianna</creatorcontrib><creatorcontrib>Sophianopoulou, Vicky</creatorcontrib><creatorcontrib>Koukaki, Marina</creatorcontrib><creatorcontrib>Scazzocchio, Claudio</creatorcontrib><creatorcontrib>Diallinas, George</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Amillis, Sotiris</au><au>Cecchetto, Gianna</au><au>Sophianopoulou, Vicky</au><au>Koukaki, Marina</au><au>Scazzocchio, Claudio</au><au>Diallinas, George</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transcription of purine transporter genes is activated during the isotropic growth phase of Aspergillus nidulans conidia</atitle><jtitle>Molecular microbiology</jtitle><addtitle>Mol Microbiol</addtitle><date>2004-04</date><risdate>2004</risdate><volume>52</volume><issue>1</issue><spage>205</spage><epage>216</epage><pages>205-216</pages><issn>0950-382X</issn><eissn>1365-2958</eissn><abstract>Summary
Aspergillus nidulans possesses three well‐characterized purine transporters encoded by the genes uapA, uapC and azgA. Expression of these genes in mycelium is induced by purines and repressed by ammonium or glutamine through the action of the pathway‐specific UaY regulator and the general GATA factor AreA respectively. Here, we describe the regulation of expression of purine transporters during conidiospore germination and the onset of mycelium development. In resting conidiospores, mRNA steady‐state levels of purine transporter genes and purine uptake activities are undetectable or very low. Both mRNA steady‐state levels and purine transport activities increase substantially during the isotropic growth phase of conidial germination. Both processes occur in the absence of purine induction and independently of the nitrogen source present in the medium. The transcriptional activator UaY is dispensable for the germination‐induced expression of the three transporter genes. AreA, on the other hand, is essential for the expression of uapA, but not for that of azgA or uapC, during germination. Transcriptional activation of uapA, uapC and azgA during germination is also independent of the presence of a carbon source in the medium. This work establishes the presence of a novel system triggering purine transporter transcription during germination. Similar results have been found in studies on the expression of other transporters in A. nidulans, suggesting that global expression of transporters might operate as a general system for sensing solute availability.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>15049821</pmid><doi>10.1046/j.1365-2958.2003.03956.x</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Molecular microbiology, 2004-04, Vol.52 (1), p.205-216 |
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| language | eng |
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| source | MEDLINE; Wiley Free Content; EZB-FREE-00999 freely available EZB journals; Wiley Online Library All Journals; Free Full-Text Journals in Chemistry |
| subjects | Aspergillus nidulans - genetics Aspergillus nidulans - growth & development Aspergillus nidulans - physiology Biological and medical sciences Blotting, Northern Fundamental and applied biological sciences. Psychology Fungal Proteins - genetics Fungal Proteins - metabolism Fungal Proteins - physiology Gene Expression Regulation, Fungal Genes, Fungal Hypoxanthine - metabolism Membrane Transport Proteins - genetics Membrane Transport Proteins - metabolism Microbiology Miscellaneous Mycelium - growth & development Mycology Nucleobase Transport Proteins - genetics Nucleobase Transport Proteins - metabolism Purines - metabolism Spores, Fungal - genetics Spores, Fungal - metabolism Trans-Activators - genetics Trans-Activators - metabolism Transcription Factors - genetics Transcription Factors - metabolism Transcription, Genetic Transcriptional Activation Xanthine - metabolism |
| title | Transcription of purine transporter genes is activated during the isotropic growth phase of Aspergillus nidulans conidia |
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