Paraffin-wax-coated plates as matrix-assisted laser desorption/ionization sample support for high-throughput identification of proteins by peptide mass fingerprinting

We compared trysin-digested protein samples desalted by ZipTip C18 reverse-phase microcolumns with on-plate washing of peptides deposited either on paraffin-coated plates (PCP), Teflon-based AnchorChip plates, or stainless steel plates, before analysis by matrix-assisted laser desorption/ionization-...

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Veröffentlicht in:	Analytical biochemistry 2004-04, Vol.327 (2), p.222-232
Hauptverfasser:	Tannu, Nilesh S, Wu, Jian, Rao, Vamshi K, Gadgil, Himanshu S, Pabst, Michael J, Gerling, Ivan C, Raghow, Rajendra
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Humans Leukocytes - chemistry Mice Myoblasts - chemistry Paraffin - chemistry Peptide Mapping - methods Proteins - analysis Proteins - isolation & purification Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - instrumentation Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods Trypsin - chemistry Waxes - chemistry
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