Analysis of Expressed Sequence Tags (ESTs) in the Ciliated Protozoan Tetrahymena thermophila

To assess the utility of expressed sequence tag (EST) sequencing as a method of gene discovery in the ciliated protozoan Tetrahymena thermophila, we have sequenced either the 5′ or 3′ ends of 157 clones chosen at random from two cDNA libraries constructed from the mRNA of vegetatively growing cultur...

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Veröffentlicht in:	The Journal of eukaryotic microbiology 2002-03, Vol.49 (2), p.99-107
Hauptverfasser:	FILLINGHAM, JEFFREY S, CHILCOAT, N. DOANE, TURKEWITZ, AARON P, ORIAS, EDUARDO, REITH, MICHAEL, PEARLMAN, RONALD E
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences cDNA library ciliate comparative genomics Databases, Nucleic Acid DNA sequencing DNA, Complementary - analysis DNA, Protozoan - analysis Expressed Sequence Tags Fundamental and applied biological sciences. Psychology gene discovery Gene Expression Regulation - physiology Gene Library genetics Genetics of eukaryotes. Biological and molecular evolution Humans molecular biology Molecular Sequence Data Population genetics, reproduction patterns Protozoa Saccharomyces cerevisiae - genetics Sequence Analysis, DNA Tetrahymena thermophila - genetics
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