Towards high performance two-dimensional gel electrophoresis using ultrazoom gels
Proteomics is the analysis of protein expression in cells or tissues, e.g., to study cellular processes at the molecular level. Ultimately, a proteome analysis should encompass most if not all protein species in a biological sample, including those present in low copy numbers. We are developing two‐...
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| Veröffentlicht in: | Electrophoresis 2000-07, Vol.21 (13), p.2617-2621 |
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| container_title | Electrophoresis |
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| creator | Hoving, Sjouke Voshol, Hans van Oostrum, Jan |
| description | Proteomics is the analysis of protein expression in cells or tissues, e.g., to study cellular processes at the molecular level. Ultimately, a proteome analysis should encompass most if not all protein species in a biological sample, including those present in low copy numbers. We are developing two‐dimensional gel electrophoresis technology by applying narrow pH range ultrazoom gels to enhance resolution and to improve the detection of low abundance proteins. Ultrazoom gels in the acidic pH range allow the detection of proteins down to 300 copies per cell of a B‐lymphoma cell line. Protein separation in the alkaline pH range, however, still requires optimization, especially in conjunction with high sample loads. |
| doi_str_mv | 10.1002/1522-2683(20000701)21:13<2617::AID-ELPS2617>3.0.CO;2-C |
| format | Article |
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Ultimately, a proteome analysis should encompass most if not all protein species in a biological sample, including those present in low copy numbers. We are developing two‐dimensional gel electrophoresis technology by applying narrow pH range ultrazoom gels to enhance resolution and to improve the detection of low abundance proteins. Ultrazoom gels in the acidic pH range allow the detection of proteins down to 300 copies per cell of a B‐lymphoma cell line. Protein separation in the alkaline pH range, however, still requires optimization, especially in conjunction with high sample loads.</description><subject>Alkaline immobilized pH gradients</subject><subject>Coloring Agents</subject><subject>Electrophoresis, Gel, Two-Dimensional - methods</subject><subject>Humans</subject><subject>Hydrogen-Ion Concentration</subject><subject>Immobilized pH gradients</subject><subject>Lymphoma, B-Cell - chemistry</subject><subject>Lymphoma, B-Cell - pathology</subject><subject>Neoplasm Proteins - isolation & purification</subject><subject>Osmolar Concentration</subject><subject>Proteome</subject><subject>Proteomics</subject><subject>Rosaniline Dyes</subject><subject>Sensitivity and Specificity</subject><subject>Silver Staining</subject><subject>Staining and Labeling - methods</subject><subject>Tumor Cells, Cultured - chemistry</subject><subject>Two-dimensional gel electrophoresis</subject><subject>Ultrazoom gels</subject><issn>0173-0835</issn><issn>1522-2683</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkE1v1DAQhi0EokvhL6CcEBy8-CtxsqBKJZRSacUWtXxIHEZex94NJPFiJ1rKr8dR2hUXDvhizej1M-MHoRNK5pQQ9pKmjGGW5fw5I_FIQl8wuqD8NcuoXCxOL97is-Xl1Vid8DmZl6tXDJf30Ozw8D6aESo5JjlPj9CjEL5HjiiEeIiOKClEQXk-Qx-v3V75KiTberNNdsZb51vVaZP0e4erujVdqF2nmmRjmsQ0Rvfe7bbOm1CHZAh1t0mGpvfqt3PtmAmP0QOrmmCe3N7H6NO7s-vyPV6uzi_K0yXWPC8kLgqj6VopyyXlVsRP88zmKa-YUEwJo0mWx2XFmthKWVtVcm2MKiqrqbVaM36Mnk3cnXc_BxN6aOugTdOozrghgKRSCkJJDH6egtq7ELyxsPN1q_wNUAKjbBidwegM7mQDo0BjGfUCRNlwJxs4EChXwKCM4Ke3Gwzr1lR_YSe7MfBtCuzrxtz859h_TD30Ih1P9Dr05teBrvwPyCSXKXz5cA751_JNSq8uQfA_AOCrJg</recordid><startdate>20000701</startdate><enddate>20000701</enddate><creator>Hoving, Sjouke</creator><creator>Voshol, Hans</creator><creator>van Oostrum, Jan</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20000701</creationdate><title>Towards high performance two-dimensional gel electrophoresis using ultrazoom gels</title><author>Hoving, Sjouke ; Voshol, Hans ; van Oostrum, Jan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3897-99ec1baaf3713f410036f853d24a2a4ec0689444b0fdaffdd7beea9dfc1ffcc23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Alkaline immobilized pH gradients</topic><topic>Coloring Agents</topic><topic>Electrophoresis, Gel, Two-Dimensional - methods</topic><topic>Humans</topic><topic>Hydrogen-Ion Concentration</topic><topic>Immobilized pH gradients</topic><topic>Lymphoma, B-Cell - chemistry</topic><topic>Lymphoma, B-Cell - pathology</topic><topic>Neoplasm Proteins - isolation & purification</topic><topic>Osmolar Concentration</topic><topic>Proteome</topic><topic>Proteomics</topic><topic>Rosaniline Dyes</topic><topic>Sensitivity and Specificity</topic><topic>Silver Staining</topic><topic>Staining and Labeling - methods</topic><topic>Tumor Cells, Cultured - chemistry</topic><topic>Two-dimensional gel electrophoresis</topic><topic>Ultrazoom gels</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hoving, Sjouke</creatorcontrib><creatorcontrib>Voshol, Hans</creatorcontrib><creatorcontrib>van Oostrum, Jan</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Electrophoresis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hoving, Sjouke</au><au>Voshol, Hans</au><au>van Oostrum, Jan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Towards high performance two-dimensional gel electrophoresis using ultrazoom gels</atitle><jtitle>Electrophoresis</jtitle><addtitle>ELECTROPHORESIS</addtitle><date>2000-07-01</date><risdate>2000</risdate><volume>21</volume><issue>13</issue><spage>2617</spage><epage>2621</epage><pages>2617-2621</pages><issn>0173-0835</issn><eissn>1522-2683</eissn><abstract>Proteomics is the analysis of protein expression in cells or tissues, e.g., to study cellular processes at the molecular level. 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| subjects | Alkaline immobilized pH gradients Coloring Agents Electrophoresis, Gel, Two-Dimensional - methods Humans Hydrogen-Ion Concentration Immobilized pH gradients Lymphoma, B-Cell - chemistry Lymphoma, B-Cell - pathology Neoplasm Proteins - isolation & purification Osmolar Concentration Proteome Proteomics Rosaniline Dyes Sensitivity and Specificity Silver Staining Staining and Labeling - methods Tumor Cells, Cultured - chemistry Two-dimensional gel electrophoresis Ultrazoom gels |
| title | Towards high performance two-dimensional gel electrophoresis using ultrazoom gels |
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