Genomic structure of the human NLK (nemo-like kinase) gene and analysis of its promoter region
Recent search revealed that nemo-like kinase (NLK) was identified as a negative regulator of the wingless type signal cascade in Xenopus and in Caenorhabditis elegans. NLK phosphorylates T-cell factor (TCF)/lymphoid enhancer-binding factor (LEF) and interferes with binding of the β-catenin-TCF/LEF c...
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| Veröffentlicht in: | Gene 2002-02, Vol.285 (1), p.175-182 |
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| creator | Harada, Haruhito Yoshida, Shoko Nobe, Yukiko Ezura, Yoichi Atake, Tomoko Koguchi, Tomoko Emi, Mitsuru |
| description | Recent search revealed that nemo-like kinase (NLK) was identified as a negative regulator of the wingless type signal cascade in
Xenopus and in
Caenorhabditis elegans. NLK phosphorylates T-cell factor (TCF)/lymphoid enhancer-binding factor (LEF) and interferes with binding of the β-catenin-TCF/LEF complex to its TCF target site. After we constructed bacterial artificial chromosome clone contig covering more than 45-kb
NLK chromosomal gene, genomic cloning revealed that the human
NLK gene consists of 11 exons interrupted by ten introns; its translation-initiation site is within exon 1, and the termination codon and polyadenylation signal lie in exon 11. The 289 amino acids of its kinase domain extend from the 3′-portion of exon 1 to the 5′-portion of exon 9, and show a high degree of similarity in amino acid sequence to kinase domains of extracellular-signal regulated kinase 5 (mitogen activated protein kinase 7) and cyclin-dependent kinases, although the positions of introns among those genes are not conserved. Reverse transcription polymerase chain reactions analysis in various tissues showed that NLK is expressed ubiquitously. Analysis of its promoter region by luciferase reporter assays in transfected HeLa and NIH3T3 cells revealed that an upstream region from −487 to +33 bp of the
NLK gene contains significant promoter activity. This 5′-flanking region probably contains the
cis-acting element of
NLK. In addition, our mutation screening showed that
NLK was not a mutational target in breast and colorectal tumor cells. |
| doi_str_mv | 10.1016/S0378-1119(02)00412-2 |
| format | Article |
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Xenopus and in
Caenorhabditis elegans. NLK phosphorylates T-cell factor (TCF)/lymphoid enhancer-binding factor (LEF) and interferes with binding of the β-catenin-TCF/LEF complex to its TCF target site. After we constructed bacterial artificial chromosome clone contig covering more than 45-kb
NLK chromosomal gene, genomic cloning revealed that the human
NLK gene consists of 11 exons interrupted by ten introns; its translation-initiation site is within exon 1, and the termination codon and polyadenylation signal lie in exon 11. The 289 amino acids of its kinase domain extend from the 3′-portion of exon 1 to the 5′-portion of exon 9, and show a high degree of similarity in amino acid sequence to kinase domains of extracellular-signal regulated kinase 5 (mitogen activated protein kinase 7) and cyclin-dependent kinases, although the positions of introns among those genes are not conserved. Reverse transcription polymerase chain reactions analysis in various tissues showed that NLK is expressed ubiquitously. Analysis of its promoter region by luciferase reporter assays in transfected HeLa and NIH3T3 cells revealed that an upstream region from −487 to +33 bp of the
NLK gene contains significant promoter activity. This 5′-flanking region probably contains the
cis-acting element of
NLK. In addition, our mutation screening showed that
NLK was not a mutational target in breast and colorectal tumor cells.</description><identifier>ISSN: 0378-1119</identifier><identifier>EISSN: 1879-0038</identifier><identifier>DOI: 10.1016/S0378-1119(02)00412-2</identifier><identifier>PMID: 12039044</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>3T3 Cells ; 5' Flanking Region - genetics ; 5′-flanking region ; Amino Acid Sequence ; Animals ; Base Sequence ; Binding Sites - genetics ; Blotting, Northern ; DNA - chemistry ; DNA - genetics ; Exons ; Female ; Gene Expression Regulation, Enzymologic ; Genes - genetics ; Genomic structure ; HeLa Cells ; Humans ; Introns ; Kinase domain ; Luciferases - genetics ; Luciferases - metabolism ; Mice ; Mitogen-Activated Protein Kinases - genetics ; Molecular Sequence Data ; Nemo-like kinase (NLK) ; nemo-like kinase gene ; Neoplasms - enzymology ; Neoplasms - genetics ; Neoplasms - pathology ; NLK gene ; Promoter ; Promoter Regions, Genetic - genetics ; Recombinant Fusion Proteins - genetics ; Recombinant Fusion Proteins - metabolism ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Sequence Alignment ; Sequence Analysis, DNA ; Sequence Homology, Amino Acid ; Tumor Cells, Cultured ; Wingless type signaling</subject><ispartof>Gene, 2002-02, Vol.285 (1), p.175-182</ispartof><rights>2002 Elsevier Science B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c392t-a708ecd6dae8468d1a07c42bc7f785b08a07fdb29b76de93e44256e717f7d9b43</citedby><cites>FETCH-LOGICAL-c392t-a708ecd6dae8468d1a07c42bc7f785b08a07fdb29b76de93e44256e717f7d9b43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0378-1119(02)00412-2$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12039044$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Harada, Haruhito</creatorcontrib><creatorcontrib>Yoshida, Shoko</creatorcontrib><creatorcontrib>Nobe, Yukiko</creatorcontrib><creatorcontrib>Ezura, Yoichi</creatorcontrib><creatorcontrib>Atake, Tomoko</creatorcontrib><creatorcontrib>Koguchi, Tomoko</creatorcontrib><creatorcontrib>Emi, Mitsuru</creatorcontrib><title>Genomic structure of the human NLK (nemo-like kinase) gene and analysis of its promoter region</title><title>Gene</title><addtitle>Gene</addtitle><description>Recent search revealed that nemo-like kinase (NLK) was identified as a negative regulator of the wingless type signal cascade in
Xenopus and in
Caenorhabditis elegans. NLK phosphorylates T-cell factor (TCF)/lymphoid enhancer-binding factor (LEF) and interferes with binding of the β-catenin-TCF/LEF complex to its TCF target site. After we constructed bacterial artificial chromosome clone contig covering more than 45-kb
NLK chromosomal gene, genomic cloning revealed that the human
NLK gene consists of 11 exons interrupted by ten introns; its translation-initiation site is within exon 1, and the termination codon and polyadenylation signal lie in exon 11. The 289 amino acids of its kinase domain extend from the 3′-portion of exon 1 to the 5′-portion of exon 9, and show a high degree of similarity in amino acid sequence to kinase domains of extracellular-signal regulated kinase 5 (mitogen activated protein kinase 7) and cyclin-dependent kinases, although the positions of introns among those genes are not conserved. Reverse transcription polymerase chain reactions analysis in various tissues showed that NLK is expressed ubiquitously. Analysis of its promoter region by luciferase reporter assays in transfected HeLa and NIH3T3 cells revealed that an upstream region from −487 to +33 bp of the
NLK gene contains significant promoter activity. This 5′-flanking region probably contains the
cis-acting element of
NLK. In addition, our mutation screening showed that
NLK was not a mutational target in breast and colorectal tumor cells.</description><subject>3T3 Cells</subject><subject>5' Flanking Region - genetics</subject><subject>5′-flanking region</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Binding Sites - genetics</subject><subject>Blotting, Northern</subject><subject>DNA - chemistry</subject><subject>DNA - genetics</subject><subject>Exons</subject><subject>Female</subject><subject>Gene Expression Regulation, Enzymologic</subject><subject>Genes - genetics</subject><subject>Genomic structure</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>Introns</subject><subject>Kinase domain</subject><subject>Luciferases - genetics</subject><subject>Luciferases - metabolism</subject><subject>Mice</subject><subject>Mitogen-Activated Protein Kinases - genetics</subject><subject>Molecular Sequence Data</subject><subject>Nemo-like kinase (NLK)</subject><subject>nemo-like kinase gene</subject><subject>Neoplasms - enzymology</subject><subject>Neoplasms - genetics</subject><subject>Neoplasms - pathology</subject><subject>NLK gene</subject><subject>Promoter</subject><subject>Promoter Regions, Genetic - genetics</subject><subject>Recombinant Fusion Proteins - genetics</subject><subject>Recombinant Fusion Proteins - metabolism</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Sequence Alignment</subject><subject>Sequence Analysis, DNA</subject><subject>Sequence Homology, Amino Acid</subject><subject>Tumor Cells, Cultured</subject><subject>Wingless type signaling</subject><issn>0378-1119</issn><issn>1879-0038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkVFPHCEUhYnR1NX2J9jwZNyHUWCYAZ6axlRturEPra8SBu4odQYUZprsv5d1N-2jJDeE3O9wknMQOqHknBLaXvwitZAVpVSdEbYkhFNWsT20oFKoipBa7qPFP-QQHeX8h5TTNOwDOqSM1IpwvkD31xDi6C3OU5rtNCfAscfTI-DHeTQB365-4LMAY6wG_wT4yQeTYYkfIAA2wZUxwzr7vFH5KePnFMc4QcIJHnwMH9FBb4YMn3b3Mbq7-vb78qZa_bz-fvl1VdlasakygkiwrnUGJG-lo4YIy1lnRS9k0xFZ3r3rmOpE60DVwDlrWhC07J3qeH2MTrf_Fv-XGfKkR58tDIMJEOesC9lKJem7IJVMCa5UAZstaFPMOUGvn5MfTVprSvSmAf3WgN7EqwnTbw1oVnSfdwZzN4L7r9pFXoAvWwBKHn89JJ2th2DB-QR20i76dyxeAcOslTo</recordid><startdate>20020220</startdate><enddate>20020220</enddate><creator>Harada, Haruhito</creator><creator>Yoshida, Shoko</creator><creator>Nobe, Yukiko</creator><creator>Ezura, Yoichi</creator><creator>Atake, Tomoko</creator><creator>Koguchi, Tomoko</creator><creator>Emi, Mitsuru</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20020220</creationdate><title>Genomic structure of the human NLK (nemo-like kinase) gene and analysis of its promoter region</title><author>Harada, Haruhito ; Yoshida, Shoko ; Nobe, Yukiko ; Ezura, Yoichi ; Atake, Tomoko ; Koguchi, Tomoko ; Emi, Mitsuru</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c392t-a708ecd6dae8468d1a07c42bc7f785b08a07fdb29b76de93e44256e717f7d9b43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>3T3 Cells</topic><topic>5' Flanking Region - genetics</topic><topic>5′-flanking region</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Binding Sites - genetics</topic><topic>Blotting, Northern</topic><topic>DNA - chemistry</topic><topic>DNA - genetics</topic><topic>Exons</topic><topic>Female</topic><topic>Gene Expression Regulation, Enzymologic</topic><topic>Genes - genetics</topic><topic>Genomic structure</topic><topic>HeLa Cells</topic><topic>Humans</topic><topic>Introns</topic><topic>Kinase domain</topic><topic>Luciferases - genetics</topic><topic>Luciferases - metabolism</topic><topic>Mice</topic><topic>Mitogen-Activated Protein Kinases - genetics</topic><topic>Molecular Sequence Data</topic><topic>Nemo-like kinase (NLK)</topic><topic>nemo-like kinase gene</topic><topic>Neoplasms - enzymology</topic><topic>Neoplasms - genetics</topic><topic>Neoplasms - pathology</topic><topic>NLK gene</topic><topic>Promoter</topic><topic>Promoter Regions, Genetic - genetics</topic><topic>Recombinant Fusion Proteins - genetics</topic><topic>Recombinant Fusion Proteins - metabolism</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Sequence Alignment</topic><topic>Sequence Analysis, DNA</topic><topic>Sequence Homology, Amino Acid</topic><topic>Tumor Cells, Cultured</topic><topic>Wingless type signaling</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Harada, Haruhito</creatorcontrib><creatorcontrib>Yoshida, Shoko</creatorcontrib><creatorcontrib>Nobe, Yukiko</creatorcontrib><creatorcontrib>Ezura, Yoichi</creatorcontrib><creatorcontrib>Atake, Tomoko</creatorcontrib><creatorcontrib>Koguchi, Tomoko</creatorcontrib><creatorcontrib>Emi, Mitsuru</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Gene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Harada, Haruhito</au><au>Yoshida, Shoko</au><au>Nobe, Yukiko</au><au>Ezura, Yoichi</au><au>Atake, Tomoko</au><au>Koguchi, Tomoko</au><au>Emi, Mitsuru</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genomic structure of the human NLK (nemo-like kinase) gene and analysis of its promoter region</atitle><jtitle>Gene</jtitle><addtitle>Gene</addtitle><date>2002-02-20</date><risdate>2002</risdate><volume>285</volume><issue>1</issue><spage>175</spage><epage>182</epage><pages>175-182</pages><issn>0378-1119</issn><eissn>1879-0038</eissn><abstract>Recent search revealed that nemo-like kinase (NLK) was identified as a negative regulator of the wingless type signal cascade in
Xenopus and in
Caenorhabditis elegans. NLK phosphorylates T-cell factor (TCF)/lymphoid enhancer-binding factor (LEF) and interferes with binding of the β-catenin-TCF/LEF complex to its TCF target site. After we constructed bacterial artificial chromosome clone contig covering more than 45-kb
NLK chromosomal gene, genomic cloning revealed that the human
NLK gene consists of 11 exons interrupted by ten introns; its translation-initiation site is within exon 1, and the termination codon and polyadenylation signal lie in exon 11. The 289 amino acids of its kinase domain extend from the 3′-portion of exon 1 to the 5′-portion of exon 9, and show a high degree of similarity in amino acid sequence to kinase domains of extracellular-signal regulated kinase 5 (mitogen activated protein kinase 7) and cyclin-dependent kinases, although the positions of introns among those genes are not conserved. Reverse transcription polymerase chain reactions analysis in various tissues showed that NLK is expressed ubiquitously. Analysis of its promoter region by luciferase reporter assays in transfected HeLa and NIH3T3 cells revealed that an upstream region from −487 to +33 bp of the
NLK gene contains significant promoter activity. This 5′-flanking region probably contains the
cis-acting element of
NLK. In addition, our mutation screening showed that
NLK was not a mutational target in breast and colorectal tumor cells.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>12039044</pmid><doi>10.1016/S0378-1119(02)00412-2</doi><tpages>8</tpages></addata></record> |
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| ispartof | Gene, 2002-02, Vol.285 (1), p.175-182 |
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| subjects | 3T3 Cells 5' Flanking Region - genetics 5′-flanking region Amino Acid Sequence Animals Base Sequence Binding Sites - genetics Blotting, Northern DNA - chemistry DNA - genetics Exons Female Gene Expression Regulation, Enzymologic Genes - genetics Genomic structure HeLa Cells Humans Introns Kinase domain Luciferases - genetics Luciferases - metabolism Mice Mitogen-Activated Protein Kinases - genetics Molecular Sequence Data Nemo-like kinase (NLK) nemo-like kinase gene Neoplasms - enzymology Neoplasms - genetics Neoplasms - pathology NLK gene Promoter Promoter Regions, Genetic - genetics Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism RNA, Messenger - genetics RNA, Messenger - metabolism Sequence Alignment Sequence Analysis, DNA Sequence Homology, Amino Acid Tumor Cells, Cultured Wingless type signaling |
| title | Genomic structure of the human NLK (nemo-like kinase) gene and analysis of its promoter region |
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