Effects of Group B Streptococci on Cord and Adult Mononuclear Cell Interleukin-12 and Interferon-γ mRNA Accumulation and Protein Secretion
Group B streptococci (GBS) are a major cause of early-onset infection in neonates. Neonates, who have defects in neutrophil function that likely contribute to susceptibility to GBS infection, are deficient in the production of the phagocyte activator interferon (IFN)—γ. GBS-stimulated mRNA accumulat...
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| Veröffentlicht in: | The Journal of infectious diseases 2000-09, Vol.182 (3), p.974-977 |
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| container_title | The Journal of infectious diseases |
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| creator | Joyner, Joanna L. Augustine, Nancy H. Taylor, Kristen A. La Pine, Timothy R. Hill, Harry R. |
| description | Group B streptococci (GBS) are a major cause of early-onset infection in neonates. Neonates, who have defects in neutrophil function that likely contribute to susceptibility to GBS infection, are deficient in the production of the phagocyte activator interferon (IFN)—γ. GBS-stimulated mRNA accumulation and protein secretion of IFN-γ and interleukin (IL)—12, a major enhancer of IFN-γ production, by mixed mononuclear cells (MMCs) from umbilical cord and adult peripheral blood was examined. GBS-exposed cord blood MMCs secreted lower concentrations of both IL-12 and IFN-γ proteins than did MMCs from adults. IL-12 and IFN-γ mRNA accumulation was examined by use of comparative reverse transcriptase—polymerase chain reaction. Cord blood MMCs accumulated less mRNA for both IL-12 and IFN-γ than did adult blood MMC. The deficiency in cord blood cell production of IL-12 may have a role in inadequate IFN-γ production, which contributes to the unique susceptibility of neonates to GBS infections. |
| doi_str_mv | 10.1086/315796 |
| format | Article |
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Neonates, who have defects in neutrophil function that likely contribute to susceptibility to GBS infection, are deficient in the production of the phagocyte activator interferon (IFN)—γ. GBS-stimulated mRNA accumulation and protein secretion of IFN-γ and interleukin (IL)—12, a major enhancer of IFN-γ production, by mixed mononuclear cells (MMCs) from umbilical cord and adult peripheral blood was examined. GBS-exposed cord blood MMCs secreted lower concentrations of both IL-12 and IFN-γ proteins than did MMCs from adults. IL-12 and IFN-γ mRNA accumulation was examined by use of comparative reverse transcriptase—polymerase chain reaction. Cord blood MMCs accumulated less mRNA for both IL-12 and IFN-γ than did adult blood MMC. The deficiency in cord blood cell production of IL-12 may have a role in inadequate IFN-γ production, which contributes to the unique susceptibility of neonates to GBS infections.</description><identifier>ISSN: 0022-1899</identifier><identifier>EISSN: 1537-6613</identifier><identifier>DOI: 10.1086/315796</identifier><identifier>PMID: 10950801</identifier><identifier>CODEN: JIDIAQ</identifier><language>eng</language><publisher>Chicago, IL: The University of Chicago Press</publisher><subject>Adult ; B lymphocytes ; Bacteriology ; Biological and medical sciences ; Blood ; Concise Communications ; Cord blood ; Culture Techniques ; Cytokines ; Fetal Blood - microbiology ; Fundamental and applied biological sciences. Psychology ; g-Interferon ; Humans ; Infant, Newborn ; Infections ; Interferon-gamma - biosynthesis ; Interferon-gamma - blood ; Interferons ; Interleukin-12 - biosynthesis ; Interleukin-12 - blood ; Messenger RNA ; Microbiology ; Monocytes - microbiology ; Neutrophils ; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains ; RNA, Messenger - blood ; Streptococcus - metabolism ; Streptococcus agalactiae ; T lymphocytes ; Time Factors ; Umbilical cord</subject><ispartof>The Journal of infectious diseases, 2000-09, Vol.182 (3), p.974-977</ispartof><rights>Copyright 2000 Infectious Diseases Society of America</rights><rights>2001 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c426t-ad9531fff1f4eb1e7aba31773cd8acb2151d08e7112390530ba99e453a88e393</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/30109309$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/30109309$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,27924,27925,58017,58250</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=959460$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10950801$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Joyner, Joanna L.</creatorcontrib><creatorcontrib>Augustine, Nancy H.</creatorcontrib><creatorcontrib>Taylor, Kristen A.</creatorcontrib><creatorcontrib>La Pine, Timothy R.</creatorcontrib><creatorcontrib>Hill, Harry R.</creatorcontrib><title>Effects of Group B Streptococci on Cord and Adult Mononuclear Cell Interleukin-12 and Interferon-γ mRNA Accumulation and Protein Secretion</title><title>The Journal of infectious diseases</title><addtitle>The Journal of Infectious Diseases</addtitle><description>Group B streptococci (GBS) are a major cause of early-onset infection in neonates. Neonates, who have defects in neutrophil function that likely contribute to susceptibility to GBS infection, are deficient in the production of the phagocyte activator interferon (IFN)—γ. GBS-stimulated mRNA accumulation and protein secretion of IFN-γ and interleukin (IL)—12, a major enhancer of IFN-γ production, by mixed mononuclear cells (MMCs) from umbilical cord and adult peripheral blood was examined. GBS-exposed cord blood MMCs secreted lower concentrations of both IL-12 and IFN-γ proteins than did MMCs from adults. IL-12 and IFN-γ mRNA accumulation was examined by use of comparative reverse transcriptase—polymerase chain reaction. Cord blood MMCs accumulated less mRNA for both IL-12 and IFN-γ than did adult blood MMC. The deficiency in cord blood cell production of IL-12 may have a role in inadequate IFN-γ production, which contributes to the unique susceptibility of neonates to GBS infections.</description><subject>Adult</subject><subject>B lymphocytes</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Blood</subject><subject>Concise Communications</subject><subject>Cord blood</subject><subject>Culture Techniques</subject><subject>Cytokines</subject><subject>Fetal Blood - microbiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>g-Interferon</subject><subject>Humans</subject><subject>Infant, Newborn</subject><subject>Infections</subject><subject>Interferon-gamma - biosynthesis</subject><subject>Interferon-gamma - blood</subject><subject>Interferons</subject><subject>Interleukin-12 - biosynthesis</subject><subject>Interleukin-12 - blood</subject><subject>Messenger RNA</subject><subject>Microbiology</subject><subject>Monocytes - microbiology</subject><subject>Neutrophils</subject><subject>Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains</subject><subject>RNA, Messenger - blood</subject><subject>Streptococcus - metabolism</subject><subject>Streptococcus agalactiae</subject><subject>T lymphocytes</subject><subject>Time Factors</subject><subject>Umbilical cord</subject><issn>0022-1899</issn><issn>1537-6613</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0dFqFDEUBuAgil2rvoESELwbzZlMksnlutS2sK3iFpHehEzmBKadnaxJBvQZfBzfw2dytrMs3nkVOOfjD-ccQl4Ceweslu85CKXlI7IAwVUhJfDHZMFYWRZQa31CnqV0xxiruFRPyQkwLVjNYEF-nXmPLicaPD2PYdzRD3STI-5ycMG5joaBrkJsqR1aumzHPtOrMIRhdD3aSFfY9_RyyBh7HO-7oYDyQT6UPMYwFH9-0-2X6yVdOjdux97mborcm88xZOwGukEXcV99Tp542yd8cXhPyc3Hs5vVRbH-dH65Wq4LV5UyF7bVgoP3HnyFDaCyjeWgFHdtbV1TgoCW1agASq6Z4KyxWmMluK1r5Jqfkrdz7C6G7yOmbLZdctMgdsAwJqNAyVpw-V8I9bTGavrlCF0MKUX0Zhe7rY0_DTCzP4-ZzzPB14fEsdli-w-b7zGBNwdgk7O9j3ZwXTo6LXQl2aRezeou5RCPXc6mHM72ExZzv0sZfxz7Nt4bqbgS5uLbrdko_lWtbyvD-V9rcq9T</recordid><startdate>20000901</startdate><enddate>20000901</enddate><creator>Joyner, Joanna L.</creator><creator>Augustine, Nancy H.</creator><creator>Taylor, Kristen A.</creator><creator>La Pine, Timothy R.</creator><creator>Hill, Harry R.</creator><general>The University of Chicago Press</general><general>University of Chicago Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T5</scope><scope>C1K</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20000901</creationdate><title>Effects of Group B Streptococci on Cord and Adult Mononuclear Cell Interleukin-12 and Interferon-γ mRNA Accumulation and Protein Secretion</title><author>Joyner, Joanna L. ; Augustine, Nancy H. ; Taylor, Kristen A. ; La Pine, Timothy R. ; Hill, Harry R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c426t-ad9531fff1f4eb1e7aba31773cd8acb2151d08e7112390530ba99e453a88e393</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Adult</topic><topic>B lymphocytes</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Blood</topic><topic>Concise Communications</topic><topic>Cord blood</topic><topic>Culture Techniques</topic><topic>Cytokines</topic><topic>Fetal Blood - microbiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>g-Interferon</topic><topic>Humans</topic><topic>Infant, Newborn</topic><topic>Infections</topic><topic>Interferon-gamma - biosynthesis</topic><topic>Interferon-gamma - blood</topic><topic>Interferons</topic><topic>Interleukin-12 - biosynthesis</topic><topic>Interleukin-12 - blood</topic><topic>Messenger RNA</topic><topic>Microbiology</topic><topic>Monocytes - microbiology</topic><topic>Neutrophils</topic><topic>Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains</topic><topic>RNA, Messenger - blood</topic><topic>Streptococcus - metabolism</topic><topic>Streptococcus agalactiae</topic><topic>T lymphocytes</topic><topic>Time Factors</topic><topic>Umbilical cord</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Joyner, Joanna L.</creatorcontrib><creatorcontrib>Augustine, Nancy H.</creatorcontrib><creatorcontrib>Taylor, Kristen A.</creatorcontrib><creatorcontrib>La Pine, Timothy R.</creatorcontrib><creatorcontrib>Hill, Harry R.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of infectious diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Joyner, Joanna L.</au><au>Augustine, Nancy H.</au><au>Taylor, Kristen A.</au><au>La Pine, Timothy R.</au><au>Hill, Harry R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of Group B Streptococci on Cord and Adult Mononuclear Cell Interleukin-12 and Interferon-γ mRNA Accumulation and Protein Secretion</atitle><jtitle>The Journal of infectious diseases</jtitle><addtitle>The Journal of Infectious Diseases</addtitle><date>2000-09-01</date><risdate>2000</risdate><volume>182</volume><issue>3</issue><spage>974</spage><epage>977</epage><pages>974-977</pages><issn>0022-1899</issn><eissn>1537-6613</eissn><coden>JIDIAQ</coden><abstract>Group B streptococci (GBS) are a major cause of early-onset infection in neonates. Neonates, who have defects in neutrophil function that likely contribute to susceptibility to GBS infection, are deficient in the production of the phagocyte activator interferon (IFN)—γ. GBS-stimulated mRNA accumulation and protein secretion of IFN-γ and interleukin (IL)—12, a major enhancer of IFN-γ production, by mixed mononuclear cells (MMCs) from umbilical cord and adult peripheral blood was examined. GBS-exposed cord blood MMCs secreted lower concentrations of both IL-12 and IFN-γ proteins than did MMCs from adults. IL-12 and IFN-γ mRNA accumulation was examined by use of comparative reverse transcriptase—polymerase chain reaction. Cord blood MMCs accumulated less mRNA for both IL-12 and IFN-γ than did adult blood MMC. 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| subjects | Adult B lymphocytes Bacteriology Biological and medical sciences Blood Concise Communications Cord blood Culture Techniques Cytokines Fetal Blood - microbiology Fundamental and applied biological sciences. Psychology g-Interferon Humans Infant, Newborn Infections Interferon-gamma - biosynthesis Interferon-gamma - blood Interferons Interleukin-12 - biosynthesis Interleukin-12 - blood Messenger RNA Microbiology Monocytes - microbiology Neutrophils Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains RNA, Messenger - blood Streptococcus - metabolism Streptococcus agalactiae T lymphocytes Time Factors Umbilical cord |
| title | Effects of Group B Streptococci on Cord and Adult Mononuclear Cell Interleukin-12 and Interferon-γ mRNA Accumulation and Protein Secretion |
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