Poly(l-lysine)-modified silica nanoparticles for the delivery of antisense oligonucleotides
Silica nanoparticles were prepared in a microemulsion system, using polyoxyethylene nonylphenyl ether/cyclohexane/ammonium hydroxide. The surface charge of the particle was modified with PLL [poly(l‐lysine)]. PAGE demonstrated the ability of PMS‐NP (PLL‐modified silica nanoparticles) to bind and pro...
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| Veröffentlicht in: | Biotechnology and applied biochemistry 2004-04, Vol.39 (2), p.179-187 |
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| creator | Zhu, Shi-Guo Xiang, Juan-Juan Li, Xiao-Ling Shen, Shou-Rong Lu, Hong-bin Zhou, Jie Xiong, Wei Zhang, Bi-Cheng Nie, Xin-Min Zhou, Ming Tang, Ke Li, Gui-Yuan |
| description | Silica nanoparticles were prepared in a microemulsion system, using polyoxyethylene nonylphenyl ether/cyclohexane/ammonium hydroxide. The surface charge of the particle was modified with PLL [poly(l‐lysine)]. PAGE demonstrated the ability of PMS‐NP (PLL‐modified silica nanoparticles) to bind and protect antisense ODNs (oligonucleotides). The intracellular localization of FITC‐labelled ODN was investigated by fluorescence microscopy. The results demonstrated that ODN could be delivered to cytoplasm. Flow‐cytometry analysis showed a 20‐fold enhancement of ODN delivered by PMS‐NP compared with free ODN for a serum‐free medium. Blocking efficacy of c‐myc antisense ODN, delivered by PMS‐NP, was examined in HNE1 and HeLa cell lines. Significant down‐regulation of c‐myc mRNA levels was observed in both the cell lines. However, the cellular uptake efficiency and antisense effects on target gene decreased in the presence of serum‐containing medium. The analysis of the filtration assay showed that PMS‐NP interacted with serum proteins. These results indicated that PMS‐NP was a suitable delivery vector for antisense ODN, although its delivery efficiency decreased in the presence of a serum‐containing medium. |
| doi_str_mv | 10.1042/BA20030077 |
| format | Article |
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The surface charge of the particle was modified with PLL [poly(l‐lysine)]. PAGE demonstrated the ability of PMS‐NP (PLL‐modified silica nanoparticles) to bind and protect antisense ODNs (oligonucleotides). The intracellular localization of FITC‐labelled ODN was investigated by fluorescence microscopy. The results demonstrated that ODN could be delivered to cytoplasm. Flow‐cytometry analysis showed a 20‐fold enhancement of ODN delivered by PMS‐NP compared with free ODN for a serum‐free medium. Blocking efficacy of c‐myc antisense ODN, delivered by PMS‐NP, was examined in HNE1 and HeLa cell lines. Significant down‐regulation of c‐myc mRNA levels was observed in both the cell lines. However, the cellular uptake efficiency and antisense effects on target gene decreased in the presence of serum‐containing medium. The analysis of the filtration assay showed that PMS‐NP interacted with serum proteins. These results indicated that PMS‐NP was a suitable delivery vector for antisense ODN, although its delivery efficiency decreased in the presence of a serum‐containing medium.</description><identifier>ISSN: 0885-4513</identifier><identifier>EISSN: 1470-8744</identifier><identifier>DOI: 10.1042/BA20030077</identifier><identifier>PMID: 15032738</identifier><identifier>CODEN: BABIEC</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>antisense delivery ; antisense oligonucleotides ; Biological and medical sciences ; Biotechnology ; Carcinoma - genetics ; Carcinoma - metabolism ; Carcinoma - pathology ; Cell Line, Tumor ; Coated Materials, Biocompatible - chemistry ; Drug Carriers - chemistry ; Fundamental and applied biological sciences. Psychology ; Gene Transfer Techniques ; HeLa Cells ; Humans ; Materials Testing ; Nanotubes - chemistry ; Nanotubes - ultrastructure ; Oligoribonucleotides, Antisense - administration & dosage ; Oligoribonucleotides, Antisense - chemistry ; Oligoribonucleotides, Antisense - genetics ; Oligoribonucleotides, Antisense - pharmacokinetics ; Particle Size ; poly(l-lysine) ; Polylysine - chemistry ; protein interaction ; silica nanoparticles ; Silicon Dioxide - chemistry ; Surface Properties ; Tissue Distribution ; Transfection - methods</subject><ispartof>Biotechnology and applied biochemistry, 2004-04, Vol.39 (2), p.179-187</ispartof><rights>2004 International Union of Biochemistry and Molecular Biology</rights><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4902-ac8bd4748c90b5c954b2c6776dfbffb7986f6f41dc88afb03ffab39909dfe633</citedby><cites>FETCH-LOGICAL-c4902-ac8bd4748c90b5c954b2c6776dfbffb7986f6f41dc88afb03ffab39909dfe633</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1042%2FBA20030077$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1042%2FBA20030077$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15660291$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15032738$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhu, Shi-Guo</creatorcontrib><creatorcontrib>Xiang, Juan-Juan</creatorcontrib><creatorcontrib>Li, Xiao-Ling</creatorcontrib><creatorcontrib>Shen, Shou-Rong</creatorcontrib><creatorcontrib>Lu, Hong-bin</creatorcontrib><creatorcontrib>Zhou, Jie</creatorcontrib><creatorcontrib>Xiong, Wei</creatorcontrib><creatorcontrib>Zhang, Bi-Cheng</creatorcontrib><creatorcontrib>Nie, Xin-Min</creatorcontrib><creatorcontrib>Zhou, Ming</creatorcontrib><creatorcontrib>Tang, Ke</creatorcontrib><creatorcontrib>Li, Gui-Yuan</creatorcontrib><title>Poly(l-lysine)-modified silica nanoparticles for the delivery of antisense oligonucleotides</title><title>Biotechnology and applied biochemistry</title><addtitle>Biotechnol Appl Biochem</addtitle><description>Silica nanoparticles were prepared in a microemulsion system, using polyoxyethylene nonylphenyl ether/cyclohexane/ammonium hydroxide. The surface charge of the particle was modified with PLL [poly(l‐lysine)]. PAGE demonstrated the ability of PMS‐NP (PLL‐modified silica nanoparticles) to bind and protect antisense ODNs (oligonucleotides). The intracellular localization of FITC‐labelled ODN was investigated by fluorescence microscopy. The results demonstrated that ODN could be delivered to cytoplasm. Flow‐cytometry analysis showed a 20‐fold enhancement of ODN delivered by PMS‐NP compared with free ODN for a serum‐free medium. Blocking efficacy of c‐myc antisense ODN, delivered by PMS‐NP, was examined in HNE1 and HeLa cell lines. Significant down‐regulation of c‐myc mRNA levels was observed in both the cell lines. However, the cellular uptake efficiency and antisense effects on target gene decreased in the presence of serum‐containing medium. The analysis of the filtration assay showed that PMS‐NP interacted with serum proteins. These results indicated that PMS‐NP was a suitable delivery vector for antisense ODN, although its delivery efficiency decreased in the presence of a serum‐containing medium.</description><subject>antisense delivery</subject><subject>antisense oligonucleotides</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Carcinoma - genetics</subject><subject>Carcinoma - metabolism</subject><subject>Carcinoma - pathology</subject><subject>Cell Line, Tumor</subject><subject>Coated Materials, Biocompatible - chemistry</subject><subject>Drug Carriers - chemistry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Transfer Techniques</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>Materials Testing</subject><subject>Nanotubes - chemistry</subject><subject>Nanotubes - ultrastructure</subject><subject>Oligoribonucleotides, Antisense - administration & dosage</subject><subject>Oligoribonucleotides, Antisense - chemistry</subject><subject>Oligoribonucleotides, Antisense - genetics</subject><subject>Oligoribonucleotides, Antisense - pharmacokinetics</subject><subject>Particle Size</subject><subject>poly(l-lysine)</subject><subject>Polylysine - chemistry</subject><subject>protein interaction</subject><subject>silica nanoparticles</subject><subject>Silicon Dioxide - chemistry</subject><subject>Surface Properties</subject><subject>Tissue Distribution</subject><subject>Transfection - methods</subject><issn>0885-4513</issn><issn>1470-8744</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0Mtu1DAUBmALgehQ2PAAKBsQIIUeX-LLsp2WgFQBEhUsWFiOL2DwxIOdKeTtCZoRZQWrcxbff470I_QQwwsMjJycnRIACiDELbTCTEArBWO30Qqk7FrWYXqE7tX6FQCkkOQuOsIdUCKoXKFP73Kan6Y2zTWO_lm7yS6G6F1TY4rWNKMZ89aUKdrkaxNyaaYvvnE-xWtf5iaHxoxTrH6svskpfs7jbpF5is7X--hOMKn6B4d5jK5eXlytX7WXb_vX69PL1jIFpDVWDo4JJq2CobOqYwOxXAjuwhDCIJTkgQeGnZXShAFoCGagSoFywXNKj9GT_dltyd93vk56E6v1KZnR513VAgsusRT_hVgorgDzBT7fQ1tyrcUHvS1xY8qsMejfleubyhf86HB1N2y8u6GHjhfw-ABMtSaFYkYb61-OcyAKL-5k737E5Od_vFzWM0w4WRLtPhHr5H_-SZjyTXNBRac_vun1h77vxfvzc72mvwB7w6an</recordid><startdate>200404</startdate><enddate>200404</enddate><creator>Zhu, Shi-Guo</creator><creator>Xiang, Juan-Juan</creator><creator>Li, Xiao-Ling</creator><creator>Shen, Shou-Rong</creator><creator>Lu, Hong-bin</creator><creator>Zhou, Jie</creator><creator>Xiong, Wei</creator><creator>Zhang, Bi-Cheng</creator><creator>Nie, Xin-Min</creator><creator>Zhou, Ming</creator><creator>Tang, Ke</creator><creator>Li, Gui-Yuan</creator><general>Blackwell Publishing Ltd</general><general>Portland Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>200404</creationdate><title>Poly(l-lysine)-modified silica nanoparticles for the delivery of antisense oligonucleotides</title><author>Zhu, Shi-Guo ; Xiang, Juan-Juan ; Li, Xiao-Ling ; Shen, Shou-Rong ; Lu, Hong-bin ; Zhou, Jie ; Xiong, Wei ; Zhang, Bi-Cheng ; Nie, Xin-Min ; Zhou, Ming ; Tang, Ke ; Li, Gui-Yuan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4902-ac8bd4748c90b5c954b2c6776dfbffb7986f6f41dc88afb03ffab39909dfe633</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>antisense delivery</topic><topic>antisense oligonucleotides</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Carcinoma - genetics</topic><topic>Carcinoma - metabolism</topic><topic>Carcinoma - pathology</topic><topic>Cell Line, Tumor</topic><topic>Coated Materials, Biocompatible - chemistry</topic><topic>Drug Carriers - chemistry</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Transfer Techniques</topic><topic>HeLa Cells</topic><topic>Humans</topic><topic>Materials Testing</topic><topic>Nanotubes - chemistry</topic><topic>Nanotubes - ultrastructure</topic><topic>Oligoribonucleotides, Antisense - administration & dosage</topic><topic>Oligoribonucleotides, Antisense - chemistry</topic><topic>Oligoribonucleotides, Antisense - genetics</topic><topic>Oligoribonucleotides, Antisense - pharmacokinetics</topic><topic>Particle Size</topic><topic>poly(l-lysine)</topic><topic>Polylysine - chemistry</topic><topic>protein interaction</topic><topic>silica nanoparticles</topic><topic>Silicon Dioxide - chemistry</topic><topic>Surface Properties</topic><topic>Tissue Distribution</topic><topic>Transfection - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhu, Shi-Guo</creatorcontrib><creatorcontrib>Xiang, Juan-Juan</creatorcontrib><creatorcontrib>Li, Xiao-Ling</creatorcontrib><creatorcontrib>Shen, Shou-Rong</creatorcontrib><creatorcontrib>Lu, Hong-bin</creatorcontrib><creatorcontrib>Zhou, Jie</creatorcontrib><creatorcontrib>Xiong, Wei</creatorcontrib><creatorcontrib>Zhang, Bi-Cheng</creatorcontrib><creatorcontrib>Nie, Xin-Min</creatorcontrib><creatorcontrib>Zhou, Ming</creatorcontrib><creatorcontrib>Tang, Ke</creatorcontrib><creatorcontrib>Li, Gui-Yuan</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biotechnology and applied biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhu, Shi-Guo</au><au>Xiang, Juan-Juan</au><au>Li, Xiao-Ling</au><au>Shen, Shou-Rong</au><au>Lu, Hong-bin</au><au>Zhou, Jie</au><au>Xiong, Wei</au><au>Zhang, Bi-Cheng</au><au>Nie, Xin-Min</au><au>Zhou, Ming</au><au>Tang, Ke</au><au>Li, Gui-Yuan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Poly(l-lysine)-modified silica nanoparticles for the delivery of antisense oligonucleotides</atitle><jtitle>Biotechnology and applied biochemistry</jtitle><addtitle>Biotechnol Appl Biochem</addtitle><date>2004-04</date><risdate>2004</risdate><volume>39</volume><issue>2</issue><spage>179</spage><epage>187</epage><pages>179-187</pages><issn>0885-4513</issn><eissn>1470-8744</eissn><coden>BABIEC</coden><abstract>Silica nanoparticles were prepared in a microemulsion system, using polyoxyethylene nonylphenyl ether/cyclohexane/ammonium hydroxide. The surface charge of the particle was modified with PLL [poly(l‐lysine)]. PAGE demonstrated the ability of PMS‐NP (PLL‐modified silica nanoparticles) to bind and protect antisense ODNs (oligonucleotides). The intracellular localization of FITC‐labelled ODN was investigated by fluorescence microscopy. The results demonstrated that ODN could be delivered to cytoplasm. Flow‐cytometry analysis showed a 20‐fold enhancement of ODN delivered by PMS‐NP compared with free ODN for a serum‐free medium. Blocking efficacy of c‐myc antisense ODN, delivered by PMS‐NP, was examined in HNE1 and HeLa cell lines. Significant down‐regulation of c‐myc mRNA levels was observed in both the cell lines. However, the cellular uptake efficiency and antisense effects on target gene decreased in the presence of serum‐containing medium. The analysis of the filtration assay showed that PMS‐NP interacted with serum proteins. These results indicated that PMS‐NP was a suitable delivery vector for antisense ODN, although its delivery efficiency decreased in the presence of a serum‐containing medium.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>15032738</pmid><doi>10.1042/BA20030077</doi><tpages>9</tpages></addata></record> |
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| ispartof | Biotechnology and applied biochemistry, 2004-04, Vol.39 (2), p.179-187 |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | antisense delivery antisense oligonucleotides Biological and medical sciences Biotechnology Carcinoma - genetics Carcinoma - metabolism Carcinoma - pathology Cell Line, Tumor Coated Materials, Biocompatible - chemistry Drug Carriers - chemistry Fundamental and applied biological sciences. Psychology Gene Transfer Techniques HeLa Cells Humans Materials Testing Nanotubes - chemistry Nanotubes - ultrastructure Oligoribonucleotides, Antisense - administration & dosage Oligoribonucleotides, Antisense - chemistry Oligoribonucleotides, Antisense - genetics Oligoribonucleotides, Antisense - pharmacokinetics Particle Size poly(l-lysine) Polylysine - chemistry protein interaction silica nanoparticles Silicon Dioxide - chemistry Surface Properties Tissue Distribution Transfection - methods |
| title | Poly(l-lysine)-modified silica nanoparticles for the delivery of antisense oligonucleotides |
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