Pinealectomy alters adipose tissue adaptability to fasting in rats
This study investigated the effects of pinealectomy and fasting on rat adipose tissue metabolism, as well as on profiles of the hormones directly involved in its regulation (insulin, leptin, and corticosterone). Pinealectomized (PINX) and sham-operated (CONTROL) adult male Wistar rats were killed 6...
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| Veröffentlicht in: | Metabolism, clinical and experimental clinical and experimental, 2004-04, Vol.53 (4), p.500-506 |
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| creator | Alonso-Vale, Maria Isabel Cardoso Anhê, Gabriel Forato Borges-Silva, Cristina das Neves Andreotti, Sandra Peres, Sidney Barnabé Cipolla-Neto, José Lima, Fabio Bessa |
| description | This study investigated the effects of pinealectomy and fasting on rat adipose tissue metabolism, as well as on profiles of the hormones directly involved in its regulation (insulin, leptin, and corticosterone). Pinealectomized (PINX) and sham-operated (CONTROL) adult male Wistar rats were killed 6 weeks after surgery, in either fed or fasted (12 and 36 hours) states. Blood samples (for glucose and hormone determinations) and peri-epididymal adipocytes (for in vitro insulin-stimulated glucose uptake, oxidation, and incorporation into lipids) were collected. Pineal ablation decreased insulin-stimulated glucose uptake in adipocytes of both fed and fasted animals without affecting insulin-binding capacity. Pinealectomy attenuated the reduction in the ability to oxidize glucose in both basal and insulin-stimulated states during fasting. This alteration in the ability of adipocytes to oxidize glucose appeared together with a decrease in insulin-induced glucose incorporation into lipids in PINX animals. Additionally, pinealectomized rats showed higher corticosterone levels in both fed and fasted states, and a lower leptinemia with 36 hours of fasting, in comparison to CONTROLs. In conclusion, our data reinforce the hypothesis that the pineal gland has a role in the modulation of adipocyte metabolism, and its absence alters metabolic adaptation to fasting in rats. |
| doi_str_mv | 10.1016/j.metabol.2003.11.009 |
| format | Article |
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Pinealectomized (PINX) and sham-operated (CONTROL) adult male Wistar rats were killed 6 weeks after surgery, in either fed or fasted (12 and 36 hours) states. Blood samples (for glucose and hormone determinations) and peri-epididymal adipocytes (for in vitro insulin-stimulated glucose uptake, oxidation, and incorporation into lipids) were collected. Pineal ablation decreased insulin-stimulated glucose uptake in adipocytes of both fed and fasted animals without affecting insulin-binding capacity. Pinealectomy attenuated the reduction in the ability to oxidize glucose in both basal and insulin-stimulated states during fasting. This alteration in the ability of adipocytes to oxidize glucose appeared together with a decrease in insulin-induced glucose incorporation into lipids in PINX animals. Additionally, pinealectomized rats showed higher corticosterone levels in both fed and fasted states, and a lower leptinemia with 36 hours of fasting, in comparison to CONTROLs. In conclusion, our data reinforce the hypothesis that the pineal gland has a role in the modulation of adipocyte metabolism, and its absence alters metabolic adaptation to fasting in rats.</description><identifier>ISSN: 0026-0495</identifier><identifier>EISSN: 1532-8600</identifier><identifier>DOI: 10.1016/j.metabol.2003.11.009</identifier><identifier>PMID: 15045699</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Adipocytes - cytology ; Adipocytes - metabolism ; Adipose Tissue - physiology ; Animals ; Biological and medical sciences ; Blood Glucose - metabolism ; Body Weight - physiology ; Carbon Dioxide - metabolism ; Cell Size ; Corticosterone - blood ; Deoxyglucose - metabolism ; Eating - physiology ; Fasting - blood ; Fasting - physiology ; Insulin - blood ; Insulin - pharmacology ; Leptin - blood ; Lipid Metabolism ; Male ; Medical sciences ; Metabolic diseases ; Obesity ; Pineal Gland - physiology ; Pineal Gland - surgery ; Rats ; Rats, Wistar</subject><ispartof>Metabolism, clinical and experimental, 2004-04, Vol.53 (4), p.500-506</ispartof><rights>2004 Elsevier Inc.</rights><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c457t-4c07f511b2975b1561df463f10f52adfbb75861d77f860ed1f0381a6bf7734f23</citedby><cites>FETCH-LOGICAL-c457t-4c07f511b2975b1561df463f10f52adfbb75861d77f860ed1f0381a6bf7734f23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0026049503005407$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15662789$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15045699$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Alonso-Vale, Maria Isabel Cardoso</creatorcontrib><creatorcontrib>Anhê, Gabriel Forato</creatorcontrib><creatorcontrib>Borges-Silva, Cristina das Neves</creatorcontrib><creatorcontrib>Andreotti, Sandra</creatorcontrib><creatorcontrib>Peres, Sidney Barnabé</creatorcontrib><creatorcontrib>Cipolla-Neto, José</creatorcontrib><creatorcontrib>Lima, Fabio Bessa</creatorcontrib><title>Pinealectomy alters adipose tissue adaptability to fasting in rats</title><title>Metabolism, clinical and experimental</title><addtitle>Metabolism</addtitle><description>This study investigated the effects of pinealectomy and fasting on rat adipose tissue metabolism, as well as on profiles of the hormones directly involved in its regulation (insulin, leptin, and corticosterone). Pinealectomized (PINX) and sham-operated (CONTROL) adult male Wistar rats were killed 6 weeks after surgery, in either fed or fasted (12 and 36 hours) states. Blood samples (for glucose and hormone determinations) and peri-epididymal adipocytes (for in vitro insulin-stimulated glucose uptake, oxidation, and incorporation into lipids) were collected. Pineal ablation decreased insulin-stimulated glucose uptake in adipocytes of both fed and fasted animals without affecting insulin-binding capacity. Pinealectomy attenuated the reduction in the ability to oxidize glucose in both basal and insulin-stimulated states during fasting. This alteration in the ability of adipocytes to oxidize glucose appeared together with a decrease in insulin-induced glucose incorporation into lipids in PINX animals. Additionally, pinealectomized rats showed higher corticosterone levels in both fed and fasted states, and a lower leptinemia with 36 hours of fasting, in comparison to CONTROLs. In conclusion, our data reinforce the hypothesis that the pineal gland has a role in the modulation of adipocyte metabolism, and its absence alters metabolic adaptation to fasting in rats.</description><subject>Adipocytes - cytology</subject><subject>Adipocytes - metabolism</subject><subject>Adipose Tissue - physiology</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Blood Glucose - metabolism</subject><subject>Body Weight - physiology</subject><subject>Carbon Dioxide - metabolism</subject><subject>Cell Size</subject><subject>Corticosterone - blood</subject><subject>Deoxyglucose - metabolism</subject><subject>Eating - physiology</subject><subject>Fasting - blood</subject><subject>Fasting - physiology</subject><subject>Insulin - blood</subject><subject>Insulin - pharmacology</subject><subject>Leptin - blood</subject><subject>Lipid Metabolism</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Metabolic diseases</subject><subject>Obesity</subject><subject>Pineal Gland - physiology</subject><subject>Pineal Gland - surgery</subject><subject>Rats</subject><subject>Rats, Wistar</subject><issn>0026-0495</issn><issn>1532-8600</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE2P1DAMhiPEih0WfgKoF7i12G2TtCfErviSVto9wDlKUwdl1I8hzqw0_56MphLcOFm2Hvu1HiHeIFQIqD7sq5mSHdapqgGaCrEC6J-JHcqmLjsF8FzsAGpVQtvLa_GSeQ8AWnfqhbhGCa1Ufb8Tt49hITuRS-t8KuyUKHJhx3BYmYoUmI-UW3vIUWEK6VSktfCWU1h-FWEpok38Slx5OzG93uqN-Pnl84-7b-X9w9fvd5_uS9dKncrWgfYScah7LQeUCkffqsYjeFnb0Q-Dll0eau3z-zSih6ZDqwavddP6urkR7y93D3H9fSROZg7saJrsQuuRjUatVK1kBuUFdHFljuTNIYbZxpNBMGd5Zm82eeYszyCaLC_vvd0CjsNM49-tzVYG3m2AZWcnH-3iAv_D5XzdnbmPF46yjqdA0bALtDgaQ8ymzbiG_7zyB1a9j6s</recordid><startdate>20040401</startdate><enddate>20040401</enddate><creator>Alonso-Vale, Maria Isabel Cardoso</creator><creator>Anhê, Gabriel Forato</creator><creator>Borges-Silva, Cristina das Neves</creator><creator>Andreotti, Sandra</creator><creator>Peres, Sidney Barnabé</creator><creator>Cipolla-Neto, José</creator><creator>Lima, Fabio Bessa</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20040401</creationdate><title>Pinealectomy alters adipose tissue adaptability to fasting in rats</title><author>Alonso-Vale, Maria Isabel Cardoso ; Anhê, Gabriel Forato ; Borges-Silva, Cristina das Neves ; Andreotti, Sandra ; Peres, Sidney Barnabé ; Cipolla-Neto, José ; Lima, Fabio Bessa</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c457t-4c07f511b2975b1561df463f10f52adfbb75861d77f860ed1f0381a6bf7734f23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Adipocytes - cytology</topic><topic>Adipocytes - metabolism</topic><topic>Adipose Tissue - physiology</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Blood Glucose - metabolism</topic><topic>Body Weight - physiology</topic><topic>Carbon Dioxide - metabolism</topic><topic>Cell Size</topic><topic>Corticosterone - blood</topic><topic>Deoxyglucose - metabolism</topic><topic>Eating - physiology</topic><topic>Fasting - blood</topic><topic>Fasting - physiology</topic><topic>Insulin - blood</topic><topic>Insulin - pharmacology</topic><topic>Leptin - blood</topic><topic>Lipid Metabolism</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Metabolic diseases</topic><topic>Obesity</topic><topic>Pineal Gland - physiology</topic><topic>Pineal Gland - surgery</topic><topic>Rats</topic><topic>Rats, Wistar</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Alonso-Vale, Maria Isabel Cardoso</creatorcontrib><creatorcontrib>Anhê, Gabriel Forato</creatorcontrib><creatorcontrib>Borges-Silva, Cristina das Neves</creatorcontrib><creatorcontrib>Andreotti, Sandra</creatorcontrib><creatorcontrib>Peres, Sidney Barnabé</creatorcontrib><creatorcontrib>Cipolla-Neto, José</creatorcontrib><creatorcontrib>Lima, Fabio Bessa</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Metabolism, clinical and experimental</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Alonso-Vale, Maria Isabel Cardoso</au><au>Anhê, Gabriel Forato</au><au>Borges-Silva, Cristina das Neves</au><au>Andreotti, Sandra</au><au>Peres, Sidney Barnabé</au><au>Cipolla-Neto, José</au><au>Lima, Fabio Bessa</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Pinealectomy alters adipose tissue adaptability to fasting in rats</atitle><jtitle>Metabolism, clinical and experimental</jtitle><addtitle>Metabolism</addtitle><date>2004-04-01</date><risdate>2004</risdate><volume>53</volume><issue>4</issue><spage>500</spage><epage>506</epage><pages>500-506</pages><issn>0026-0495</issn><eissn>1532-8600</eissn><abstract>This study investigated the effects of pinealectomy and fasting on rat adipose tissue metabolism, as well as on profiles of the hormones directly involved in its regulation (insulin, leptin, and corticosterone). Pinealectomized (PINX) and sham-operated (CONTROL) adult male Wistar rats were killed 6 weeks after surgery, in either fed or fasted (12 and 36 hours) states. Blood samples (for glucose and hormone determinations) and peri-epididymal adipocytes (for in vitro insulin-stimulated glucose uptake, oxidation, and incorporation into lipids) were collected. Pineal ablation decreased insulin-stimulated glucose uptake in adipocytes of both fed and fasted animals without affecting insulin-binding capacity. Pinealectomy attenuated the reduction in the ability to oxidize glucose in both basal and insulin-stimulated states during fasting. This alteration in the ability of adipocytes to oxidize glucose appeared together with a decrease in insulin-induced glucose incorporation into lipids in PINX animals. Additionally, pinealectomized rats showed higher corticosterone levels in both fed and fasted states, and a lower leptinemia with 36 hours of fasting, in comparison to CONTROLs. In conclusion, our data reinforce the hypothesis that the pineal gland has a role in the modulation of adipocyte metabolism, and its absence alters metabolic adaptation to fasting in rats.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>15045699</pmid><doi>10.1016/j.metabol.2003.11.009</doi><tpages>7</tpages></addata></record> |
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| subjects | Adipocytes - cytology Adipocytes - metabolism Adipose Tissue - physiology Animals Biological and medical sciences Blood Glucose - metabolism Body Weight - physiology Carbon Dioxide - metabolism Cell Size Corticosterone - blood Deoxyglucose - metabolism Eating - physiology Fasting - blood Fasting - physiology Insulin - blood Insulin - pharmacology Leptin - blood Lipid Metabolism Male Medical sciences Metabolic diseases Obesity Pineal Gland - physiology Pineal Gland - surgery Rats Rats, Wistar |
| title | Pinealectomy alters adipose tissue adaptability to fasting in rats |
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