Presence, infectivity, and stability of enteric viruses in seawater: relationship to marine water quality in the Florida Keys
Concerns about the presence of enteric viruses in the surface waters of the Florida Keys prompted analyses of virus stability and persistence in these waters. In an in vitro study we evaluated the survival of poliovirus and stability of viral RNA in filtered natural seawater (FSW), unfiltered natura...
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| Veröffentlicht in: | Marine pollution bulletin 2004-04, Vol.48 (7), p.698-704 |
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| creator | Wetz, Jennifer Jarrell Lipp, Erin K Griffin, Dale W Lukasik, Jerzy Wait, Douglas Sobsey, Mark D Scott, Troy M Rose, Joan B |
| description | Concerns about the presence of enteric viruses in the surface waters of the Florida Keys prompted analyses of virus stability and persistence in these waters. In an in vitro study we evaluated the survival of poliovirus and stability of viral RNA in filtered natural seawater (FSW), unfiltered natural seawater (USW), artificial seawater (ASW) and DI water. This study compared cell culture infectivity with direct reverse transcription–polymerase chain reaction analysis. Attenuated poliovirus was seeded in the above water types and incubated in the dark at 22 and 30 °C for 60 days. At 22 °C, enhanced poliovirus survival and enhanced detection of viral RNA was observed in the seeded DI water control, artificial seawater and FSW samples. Detection of viruses in unfiltered seawater decreased rapidly at both temperatures by both methods of detection, suggesting that in the natural environment detection of enteroviral RNA may indicate a recent contamination event. In addition, in situ sampling in the Florida Keys during the late winter of 2000 revealed the presence of infectious enteroviruses at two sites and no sites exceeded recommended levels of microbial water quality indicators (enterococci or fecal coliform bacteria). |
| doi_str_mv | 10.1016/j.marpolbul.2003.09.008 |
| format | Article |
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In an in vitro study we evaluated the survival of poliovirus and stability of viral RNA in filtered natural seawater (FSW), unfiltered natural seawater (USW), artificial seawater (ASW) and DI water. This study compared cell culture infectivity with direct reverse transcription–polymerase chain reaction analysis. Attenuated poliovirus was seeded in the above water types and incubated in the dark at 22 and 30 °C for 60 days. At 22 °C, enhanced poliovirus survival and enhanced detection of viral RNA was observed in the seeded DI water control, artificial seawater and FSW samples. Detection of viruses in unfiltered seawater decreased rapidly at both temperatures by both methods of detection, suggesting that in the natural environment detection of enteroviral RNA may indicate a recent contamination event. In addition, in situ sampling in the Florida Keys during the late winter of 2000 revealed the presence of infectious enteroviruses at two sites and no sites exceeded recommended levels of microbial water quality indicators (enterococci or fecal coliform bacteria).</description><identifier>ISSN: 0025-326X</identifier><identifier>EISSN: 1879-3363</identifier><identifier>DOI: 10.1016/j.marpolbul.2003.09.008</identifier><identifier>PMID: 15041426</identifier><identifier>CODEN: MPNBAZ</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Air. Soil. Water. Waste. Feeding ; Animal, plant and microbial ecology ; Applied sciences ; Bacteria ; Biological and medical sciences ; Earth sciences ; Earth, ocean, space ; Engineering and environment geology. Geothermics ; Enterobacteriaceae - physiology ; Enterovirus ; Enteroviruses ; Environment. Living conditions ; Exact sciences and technology ; Fecal indicators ; Florida ; Florida Keys ; Fundamental and applied biological sciences. Psychology ; Marine ; Medical sciences ; Microbial ecology ; Natural water pollution ; Poliovirus ; Poliovirus - pathogenicity ; Poliovirus - physiology ; Pollution ; Pollution, environment geology ; Public health. Hygiene ; Public health. Hygiene-occupational medicine ; Reverse Transcriptase Polymerase Chain Reaction ; RNA ; RNA, Viral - genetics ; Seawater - microbiology ; Seawater - virology ; Seawaters, estuaries ; Sewage ; Temperature ; Various environments (extraatmospheric space, air, water) ; Viral Plaque Assay ; Water treatment and pollution</subject><ispartof>Marine pollution bulletin, 2004-04, Vol.48 (7), p.698-704</ispartof><rights>2003 Elsevier Ltd</rights><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c554t-8e963728f4ec976b817c3b6edd30f984a7616c01b535f97bd35757e9453876d13</citedby><cites>FETCH-LOGICAL-c554t-8e963728f4ec976b817c3b6edd30f984a7616c01b535f97bd35757e9453876d13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.marpolbul.2003.09.008$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15621319$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15041426$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wetz, Jennifer Jarrell</creatorcontrib><creatorcontrib>Lipp, Erin K</creatorcontrib><creatorcontrib>Griffin, Dale W</creatorcontrib><creatorcontrib>Lukasik, Jerzy</creatorcontrib><creatorcontrib>Wait, Douglas</creatorcontrib><creatorcontrib>Sobsey, Mark D</creatorcontrib><creatorcontrib>Scott, Troy M</creatorcontrib><creatorcontrib>Rose, Joan B</creatorcontrib><title>Presence, infectivity, and stability of enteric viruses in seawater: relationship to marine water quality in the Florida Keys</title><title>Marine pollution bulletin</title><addtitle>Mar Pollut Bull</addtitle><description>Concerns about the presence of enteric viruses in the surface waters of the Florida Keys prompted analyses of virus stability and persistence in these waters. In an in vitro study we evaluated the survival of poliovirus and stability of viral RNA in filtered natural seawater (FSW), unfiltered natural seawater (USW), artificial seawater (ASW) and DI water. This study compared cell culture infectivity with direct reverse transcription–polymerase chain reaction analysis. Attenuated poliovirus was seeded in the above water types and incubated in the dark at 22 and 30 °C for 60 days. At 22 °C, enhanced poliovirus survival and enhanced detection of viral RNA was observed in the seeded DI water control, artificial seawater and FSW samples. Detection of viruses in unfiltered seawater decreased rapidly at both temperatures by both methods of detection, suggesting that in the natural environment detection of enteroviral RNA may indicate a recent contamination event. In addition, in situ sampling in the Florida Keys during the late winter of 2000 revealed the presence of infectious enteroviruses at two sites and no sites exceeded recommended levels of microbial water quality indicators (enterococci or fecal coliform bacteria).</description><subject>Air. Soil. Water. Waste. Feeding</subject><subject>Animal, plant and microbial ecology</subject><subject>Applied sciences</subject><subject>Bacteria</subject><subject>Biological and medical sciences</subject><subject>Earth sciences</subject><subject>Earth, ocean, space</subject><subject>Engineering and environment geology. Geothermics</subject><subject>Enterobacteriaceae - physiology</subject><subject>Enterovirus</subject><subject>Enteroviruses</subject><subject>Environment. Living conditions</subject><subject>Exact sciences and technology</subject><subject>Fecal indicators</subject><subject>Florida</subject><subject>Florida Keys</subject><subject>Fundamental and applied biological sciences. 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Hygiene</topic><topic>Public health. Hygiene-occupational medicine</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA</topic><topic>RNA, Viral - genetics</topic><topic>Seawater - microbiology</topic><topic>Seawater - virology</topic><topic>Seawaters, estuaries</topic><topic>Sewage</topic><topic>Temperature</topic><topic>Various environments (extraatmospheric space, air, water)</topic><topic>Viral Plaque Assay</topic><topic>Water treatment and pollution</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wetz, Jennifer Jarrell</creatorcontrib><creatorcontrib>Lipp, Erin K</creatorcontrib><creatorcontrib>Griffin, Dale W</creatorcontrib><creatorcontrib>Lukasik, Jerzy</creatorcontrib><creatorcontrib>Wait, Douglas</creatorcontrib><creatorcontrib>Sobsey, Mark D</creatorcontrib><creatorcontrib>Scott, Troy M</creatorcontrib><creatorcontrib>Rose, Joan B</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Environment Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Environment Abstracts</collection><collection>Aqualine</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Oceanic Abstracts</collection><collection>Pollution Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Water Resources Abstracts</collection><collection>Technology Research Database</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Marine pollution bulletin</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wetz, Jennifer Jarrell</au><au>Lipp, Erin K</au><au>Griffin, Dale W</au><au>Lukasik, Jerzy</au><au>Wait, Douglas</au><au>Sobsey, Mark D</au><au>Scott, Troy M</au><au>Rose, Joan B</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Presence, infectivity, and stability of enteric viruses in seawater: relationship to marine water quality in the Florida Keys</atitle><jtitle>Marine pollution bulletin</jtitle><addtitle>Mar Pollut Bull</addtitle><date>2004-04-01</date><risdate>2004</risdate><volume>48</volume><issue>7</issue><spage>698</spage><epage>704</epage><pages>698-704</pages><issn>0025-326X</issn><eissn>1879-3363</eissn><coden>MPNBAZ</coden><abstract>Concerns about the presence of enteric viruses in the surface waters of the Florida Keys prompted analyses of virus stability and persistence in these waters. In an in vitro study we evaluated the survival of poliovirus and stability of viral RNA in filtered natural seawater (FSW), unfiltered natural seawater (USW), artificial seawater (ASW) and DI water. This study compared cell culture infectivity with direct reverse transcription–polymerase chain reaction analysis. Attenuated poliovirus was seeded in the above water types and incubated in the dark at 22 and 30 °C for 60 days. At 22 °C, enhanced poliovirus survival and enhanced detection of viral RNA was observed in the seeded DI water control, artificial seawater and FSW samples. Detection of viruses in unfiltered seawater decreased rapidly at both temperatures by both methods of detection, suggesting that in the natural environment detection of enteroviral RNA may indicate a recent contamination event. In addition, in situ sampling in the Florida Keys during the late winter of 2000 revealed the presence of infectious enteroviruses at two sites and no sites exceeded recommended levels of microbial water quality indicators (enterococci or fecal coliform bacteria).</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>15041426</pmid><doi>10.1016/j.marpolbul.2003.09.008</doi><tpages>7</tpages></addata></record> |
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| ispartof | Marine pollution bulletin, 2004-04, Vol.48 (7), p.698-704 |
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| subjects | Air. Soil. Water. Waste. Feeding Animal, plant and microbial ecology Applied sciences Bacteria Biological and medical sciences Earth sciences Earth, ocean, space Engineering and environment geology. Geothermics Enterobacteriaceae - physiology Enterovirus Enteroviruses Environment. Living conditions Exact sciences and technology Fecal indicators Florida Florida Keys Fundamental and applied biological sciences. Psychology Marine Medical sciences Microbial ecology Natural water pollution Poliovirus Poliovirus - pathogenicity Poliovirus - physiology Pollution Pollution, environment geology Public health. Hygiene Public health. Hygiene-occupational medicine Reverse Transcriptase Polymerase Chain Reaction RNA RNA, Viral - genetics Seawater - microbiology Seawater - virology Seawaters, estuaries Sewage Temperature Various environments (extraatmospheric space, air, water) Viral Plaque Assay Water treatment and pollution |
| title | Presence, infectivity, and stability of enteric viruses in seawater: relationship to marine water quality in the Florida Keys |
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