Immunohistochemical Demonstration of Cl- Pump in Type A Intercalated Cells of Rat Kidney
In order to demonstrate the localization of an ethacrynic acid−sensitive Cl- pump in the rat kidney, immunohistochemical analysis was performed using an anti−Cl- pump antibody raised against rat brain Cl- pump protein with confocal laser scanning microscopy.The antibodies against Na+, K+−ATPase, aqu...
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| Veröffentlicht in: | Japanese Journal of Pharmacology 2000, Vol.83(3), pp.233-240 |
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| container_title | Japanese Journal of Pharmacology |
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| creator | Higashida, Toshiaki Zeng, Xun-Ting Hara, Mitsuyoshi Omori, Koichiro Sasaki, Sei Orikasa, Michiaki Shimizu, Fujio Inagaki, Chiyoko |
| description | In order to demonstrate the localization of an ethacrynic acid−sensitive Cl- pump in the rat kidney, immunohistochemical analysis was performed using an anti−Cl- pump antibody raised against rat brain Cl- pump protein with confocal laser scanning microscopy.The antibodies against Na+, K+−ATPase, aquaporin 2 and a type B intercalated cell marker, 43−kDa protein, were also used for comparison.Anti−Cl- pump antibody recognized a 51−kDa renal protein of the same size as that in the brain on Western blots.Cl-−pump−like immunoreactivity was observed on the basolateral membranes of 42±3% of cortical collecting duct(CCD)cells and of 38±1% of outer medullar collecting duct(OMCD)cells.Such immunoreactivity in CCD was sometimes co−localized with Na+, K+−ATPase, but in OMCD, the Cl- pump−like immunoreactivity co−existed with neither Na+, K+−ATPase, aquaporin 2 nor the type B intercalated cell marker 43−kDa protein.Thus, the Cl- pump was demonstrated to be localized on the basolateral membranes of type A intercalated cells of cortical and medullary collecting ducts in the rat kidney. |
| doi_str_mv | 10.1254/jjp.83.233 |
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metabolism ; Blotting, Western ; Carrier Proteins - metabolism ; Chlorides - metabolism ; Cl- pump ; Collecting duct ; Ethacrynic acid ; Immunohistochemistry ; Intercalated cell ; Kidney ; Kidney - cytology ; Kidney - enzymology ; Kidney - metabolism ; Male ; Rats ; Rats, Wistar ; Sodium-Potassium-Exchanging ATPase - metabolism</subject><ispartof>The Japanese Journal of Pharmacology, 2000, Vol.83(3), pp.233-240</ispartof><rights>The Japanese Pharmacological Society 2000</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c630t-740d27b35b31cecf28a8c0f5f3ccf18a10b6f78b9c544ea8a10468be99f057c63</citedby><cites>FETCH-LOGICAL-c630t-740d27b35b31cecf28a8c0f5f3ccf18a10b6f78b9c544ea8a10468be99f057c63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1876,4009,27902,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10952072$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Higashida, Toshiaki</creatorcontrib><creatorcontrib>Zeng, Xun-Ting</creatorcontrib><creatorcontrib>Hara, Mitsuyoshi</creatorcontrib><creatorcontrib>Omori, Koichiro</creatorcontrib><creatorcontrib>Sasaki, Sei</creatorcontrib><creatorcontrib>Orikasa, Michiaki</creatorcontrib><creatorcontrib>Shimizu, Fujio</creatorcontrib><creatorcontrib>Inagaki, Chiyoko</creatorcontrib><creatorcontrib>Institute of Nephrology</creatorcontrib><creatorcontrib>Tokyo Medical and Dental University</creatorcontrib><creatorcontrib>Department of Pharmacology</creatorcontrib><creatorcontrib>Second Internal Medicine</creatorcontrib><creatorcontrib>Niigata University School of Medicine</creatorcontrib><creatorcontrib>Department of Physiology</creatorcontrib><creatorcontrib>Department of Cell Biology</creatorcontrib><creatorcontrib>Kansai Medical University</creatorcontrib><title>Immunohistochemical Demonstration of Cl- Pump in Type A Intercalated Cells of Rat Kidney</title><title>Japanese Journal of Pharmacology</title><addtitle>Jpn.J.Pharmacol.</addtitle><description>In order to demonstrate the localization of an ethacrynic acid−sensitive Cl- pump in the rat kidney, immunohistochemical analysis was performed using an anti−Cl- pump antibody raised against rat brain Cl- pump protein with confocal laser scanning microscopy.The antibodies against Na+, K+−ATPase, aquaporin 2 and a type B intercalated cell marker, 43−kDa protein, were also used for comparison.Anti−Cl- pump antibody recognized a 51−kDa renal protein of the same size as that in the brain on Western blots.Cl-−pump−like immunoreactivity was observed on the basolateral membranes of 42±3% of cortical collecting duct(CCD)cells and of 38±1% of outer medullar collecting duct(OMCD)cells.Such immunoreactivity in CCD was sometimes co−localized with Na+, K+−ATPase, but in OMCD, the Cl- pump−like immunoreactivity co−existed with neither Na+, K+−ATPase, aquaporin 2 nor the type B intercalated cell marker 43−kDa protein.Thus, the Cl- pump was demonstrated to be localized on the basolateral membranes of type A intercalated cells of cortical and medullary collecting ducts in the rat kidney.</description><subject>Animals</subject><subject>Aquaporin 2</subject><subject>Aquaporin 6</subject><subject>Aquaporins - metabolism</subject><subject>Blotting, Western</subject><subject>Carrier Proteins - metabolism</subject><subject>Chlorides - metabolism</subject><subject>Cl- pump</subject><subject>Collecting duct</subject><subject>Ethacrynic acid</subject><subject>Immunohistochemistry</subject><subject>Intercalated cell</subject><subject>Kidney</subject><subject>Kidney - cytology</subject><subject>Kidney - enzymology</subject><subject>Kidney - metabolism</subject><subject>Male</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Sodium-Potassium-Exchanging ATPase - metabolism</subject><issn>0021-5198</issn><issn>1347-3506</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkE9v1DAQxS0EokvhwgdAPnFAyjKO88c5oWqhsKISCBWJm-V4x6yjxA62c9hvj6O0iMuM7Pn5zfMj5DWDPSvr6v0wzHvB9yXnT8iO8aoteA3NU7IDKFlRs05ckRcxDvkogFXPyRWDri6hLXfk13GaFufPNiavzzhZrUb6ESfvYgoqWe-oN_QwFvT7Ms3UOnp_mZHe0KNLGDKsEp7oAccxruAPlehXe3J4eUmeGTVGfPXQr8nP20_3hy_F3bfPx8PNXaEbDqloKziVbc_rnjON2pRCCQ2mNlxrw4Ri0DemFX2n66pCtV5Ujeix6wzUbda4Jm833Tn4PwvGJCcbdfajHPolypa1DcurMvhuA3XwMQY0cg52UuEiGcg1R5lzlILLnGOG3zyoLv2Ep__QLbgM3G5Anq6ZeTdah3LwS3D5u1KbZhj8PMoSACSAyA5y6yRk-Vwq4KyBrspCHzahISb1G_9tUiFZPeKjKb6V9fHjRJ9VkOj4X_3Omr4</recordid><startdate>2000</startdate><enddate>2000</enddate><creator>Higashida, Toshiaki</creator><creator>Zeng, Xun-Ting</creator><creator>Hara, Mitsuyoshi</creator><creator>Omori, Koichiro</creator><creator>Sasaki, Sei</creator><creator>Orikasa, Michiaki</creator><creator>Shimizu, Fujio</creator><creator>Inagaki, Chiyoko</creator><general>The Japanese Pharmacological Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>2000</creationdate><title>Immunohistochemical Demonstration of Cl- Pump in Type A Intercalated Cells of Rat Kidney</title><author>Higashida, Toshiaki ; Zeng, Xun-Ting ; Hara, Mitsuyoshi ; Omori, Koichiro ; Sasaki, Sei ; Orikasa, Michiaki ; Shimizu, Fujio ; Inagaki, Chiyoko</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c630t-740d27b35b31cecf28a8c0f5f3ccf18a10b6f78b9c544ea8a10468be99f057c63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Animals</topic><topic>Aquaporin 2</topic><topic>Aquaporin 6</topic><topic>Aquaporins - metabolism</topic><topic>Blotting, Western</topic><topic>Carrier Proteins - metabolism</topic><topic>Chlorides - metabolism</topic><topic>Cl- pump</topic><topic>Collecting duct</topic><topic>Ethacrynic acid</topic><topic>Immunohistochemistry</topic><topic>Intercalated cell</topic><topic>Kidney</topic><topic>Kidney - cytology</topic><topic>Kidney - enzymology</topic><topic>Kidney - metabolism</topic><topic>Male</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Sodium-Potassium-Exchanging ATPase - metabolism</topic><toplevel>online_resources</toplevel><creatorcontrib>Higashida, Toshiaki</creatorcontrib><creatorcontrib>Zeng, Xun-Ting</creatorcontrib><creatorcontrib>Hara, Mitsuyoshi</creatorcontrib><creatorcontrib>Omori, Koichiro</creatorcontrib><creatorcontrib>Sasaki, Sei</creatorcontrib><creatorcontrib>Orikasa, Michiaki</creatorcontrib><creatorcontrib>Shimizu, Fujio</creatorcontrib><creatorcontrib>Inagaki, Chiyoko</creatorcontrib><creatorcontrib>Institute of Nephrology</creatorcontrib><creatorcontrib>Tokyo Medical and Dental University</creatorcontrib><creatorcontrib>Department of Pharmacology</creatorcontrib><creatorcontrib>Second Internal Medicine</creatorcontrib><creatorcontrib>Niigata University School of Medicine</creatorcontrib><creatorcontrib>Department of Physiology</creatorcontrib><creatorcontrib>Department of Cell Biology</creatorcontrib><creatorcontrib>Kansai Medical University</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - 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| subjects | Animals Aquaporin 2 Aquaporin 6 Aquaporins - metabolism Blotting, Western Carrier Proteins - metabolism Chlorides - metabolism Cl- pump Collecting duct Ethacrynic acid Immunohistochemistry Intercalated cell Kidney Kidney - cytology Kidney - enzymology Kidney - metabolism Male Rats Rats, Wistar Sodium-Potassium-Exchanging ATPase - metabolism |
| title | Immunohistochemical Demonstration of Cl- Pump in Type A Intercalated Cells of Rat Kidney |
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