The Identification of Endothelial Cell Autoantigens
The identity of many endothelial cell autoantigens remains unclear. This study has used human monoclonal anti-endothelial cell autoantibodies isolated from patients with SLE to identify endothelial autoantigens. Thirteen antibodies reactive with endothelial cell membrane preparations were isolated a...
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| Veröffentlicht in: | Journal of autoimmunity 2000-08, Vol.15 (1), p.41-49 |
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| container_end_page | 49 |
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| container_title | Journal of autoimmunity |
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| creator | Yazici, Z.A Behrendt, M Cooper, D Goodfield, M Partridge, L Lindsey, N.J |
| description | The identity of many endothelial cell autoantigens remains unclear. This study has used human monoclonal anti-endothelial cell autoantibodies isolated from patients with SLE to identify endothelial autoantigens. Thirteen antibodies reactive with endothelial cell membrane preparations were isolated and cloned, one of which has previously been demonstrated to be pro-inflammatory. Western blotting demonstrates that these antibodies recognize a variety of proteins in endothelial cell membrane preparations. Further characterization of five antibodies by cDNA library screening, immunofluorescence and Western blotting proves that two of these antibodies recognized the cytoskeletal proteins tubulin and vimentin. A further antibody identified a clone derived from human collagenase, an identification supported by Western blotting. The multiple clones selected by other antibodies are not compatible with the molecular weight of the antigen recognized in Western blotting studies. This study has clearly identified two endothelial cell autoantigens present in membrane preparations and provides strong evidence as to the identity of a third. |
| doi_str_mv | 10.1006/jaut.2000.0391 |
| format | Article |
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This study has used human monoclonal anti-endothelial cell autoantibodies isolated from patients with SLE to identify endothelial autoantigens. Thirteen antibodies reactive with endothelial cell membrane preparations were isolated and cloned, one of which has previously been demonstrated to be pro-inflammatory. Western blotting demonstrates that these antibodies recognize a variety of proteins in endothelial cell membrane preparations. Further characterization of five antibodies by cDNA library screening, immunofluorescence and Western blotting proves that two of these antibodies recognized the cytoskeletal proteins tubulin and vimentin. A further antibody identified a clone derived from human collagenase, an identification supported by Western blotting. The multiple clones selected by other antibodies are not compatible with the molecular weight of the antigen recognized in Western blotting studies. 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This study has used human monoclonal anti-endothelial cell autoantibodies isolated from patients with SLE to identify endothelial autoantigens. Thirteen antibodies reactive with endothelial cell membrane preparations were isolated and cloned, one of which has previously been demonstrated to be pro-inflammatory. Western blotting demonstrates that these antibodies recognize a variety of proteins in endothelial cell membrane preparations. Further characterization of five antibodies by cDNA library screening, immunofluorescence and Western blotting proves that two of these antibodies recognized the cytoskeletal proteins tubulin and vimentin. A further antibody identified a clone derived from human collagenase, an identification supported by Western blotting. The multiple clones selected by other antibodies are not compatible with the molecular weight of the antigen recognized in Western blotting studies. This study has clearly identified two endothelial cell autoantigens present in membrane preparations and provides strong evidence as to the identity of a third.</description><subject>Antibodies, Monoclonal - metabolism</subject><subject>Antigen-Antibody Reactions</subject><subject>autoantibody, endothelium, cDNA libraries</subject><subject>Autoantigens - analysis</subject><subject>Autoantigens - immunology</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Cell Line</subject><subject>DNA, Complementary - analysis</subject><subject>Endothelium, Vascular - chemistry</subject><subject>Endothelium, Vascular - cytology</subject><subject>Endothelium, Vascular - immunology</subject><subject>Fluorescent Antibody Technique, Indirect</subject><subject>Gene Library</subject><subject>General aspects</subject><subject>Humans</subject><subject>Immunopathology</subject><subject>K562 Cells</subject><subject>Medical sciences</subject><subject>Molecular Weight</subject><issn>0896-8411</issn><issn>1095-9157</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0D1PwzAQgGELgWgprIwoA2JLucuX47GqClSqxFJmy3Eu1FUalzhB4t8TK5VgQUxeHp_uXsZuEeYIkD3uVd_NIwCYQyzwjE0RRBoKTPk5m0IusjBPECfsyrk9AGKappdsMqA4g4hPWbzdUbAuqelMZbTqjG0CWwWrprTdjmqj6mBJdR0s-s6qAb1T467ZRaVqRzend8benlbb5Uu4eX1eLxebUCci7UIqOcU8wSxOhBZpISINmkSsUOlMJZRjlBS51hwSSquKoqLEMhIQFbyIK57HM_Ywzj229qMn18mDcXrYRjVkeyc5cn8E_gu9yxE8nI9Qt9a5lip5bM1BtV8SQfqe0veUvqf0PYcPd6fJfXGg8hcfAw7g_gSU06quWtVo435cMtyT-zn5yGjo9WmolU4bajSVpiXdydKav1b4Bqpcj8s</recordid><startdate>20000801</startdate><enddate>20000801</enddate><creator>Yazici, Z.A</creator><creator>Behrendt, M</creator><creator>Cooper, D</creator><creator>Goodfield, M</creator><creator>Partridge, L</creator><creator>Lindsey, N.J</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20000801</creationdate><title>The Identification of Endothelial Cell Autoantigens</title><author>Yazici, Z.A ; Behrendt, M ; Cooper, D ; Goodfield, M ; Partridge, L ; Lindsey, N.J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c495t-ed7e37416349c95b92c0ce93a1ac6a4e8124b8cc704e5ffe2bd1d2902b7b3f783</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Antibodies, Monoclonal - metabolism</topic><topic>Antigen-Antibody Reactions</topic><topic>autoantibody, endothelium, cDNA libraries</topic><topic>Autoantigens - analysis</topic><topic>Autoantigens - immunology</topic><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>Cell Line</topic><topic>DNA, Complementary - analysis</topic><topic>Endothelium, Vascular - chemistry</topic><topic>Endothelium, Vascular - cytology</topic><topic>Endothelium, Vascular - immunology</topic><topic>Fluorescent Antibody Technique, Indirect</topic><topic>Gene Library</topic><topic>General aspects</topic><topic>Humans</topic><topic>Immunopathology</topic><topic>K562 Cells</topic><topic>Medical sciences</topic><topic>Molecular Weight</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yazici, Z.A</creatorcontrib><creatorcontrib>Behrendt, M</creatorcontrib><creatorcontrib>Cooper, D</creatorcontrib><creatorcontrib>Goodfield, M</creatorcontrib><creatorcontrib>Partridge, L</creatorcontrib><creatorcontrib>Lindsey, N.J</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of autoimmunity</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yazici, Z.A</au><au>Behrendt, M</au><au>Cooper, D</au><au>Goodfield, M</au><au>Partridge, L</au><au>Lindsey, N.J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Identification of Endothelial Cell Autoantigens</atitle><jtitle>Journal of autoimmunity</jtitle><addtitle>J Autoimmun</addtitle><date>2000-08-01</date><risdate>2000</risdate><volume>15</volume><issue>1</issue><spage>41</spage><epage>49</epage><pages>41-49</pages><issn>0896-8411</issn><eissn>1095-9157</eissn><abstract>The identity of many endothelial cell autoantigens remains unclear. This study has used human monoclonal anti-endothelial cell autoantibodies isolated from patients with SLE to identify endothelial autoantigens. Thirteen antibodies reactive with endothelial cell membrane preparations were isolated and cloned, one of which has previously been demonstrated to be pro-inflammatory. Western blotting demonstrates that these antibodies recognize a variety of proteins in endothelial cell membrane preparations. Further characterization of five antibodies by cDNA library screening, immunofluorescence and Western blotting proves that two of these antibodies recognized the cytoskeletal proteins tubulin and vimentin. A further antibody identified a clone derived from human collagenase, an identification supported by Western blotting. The multiple clones selected by other antibodies are not compatible with the molecular weight of the antigen recognized in Western blotting studies. This study has clearly identified two endothelial cell autoantigens present in membrane preparations and provides strong evidence as to the identity of a third.</abstract><cop>London</cop><pub>Elsevier Ltd</pub><pmid>10936027</pmid><doi>10.1006/jaut.2000.0391</doi><tpages>9</tpages></addata></record> |
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| subjects | Antibodies, Monoclonal - metabolism Antigen-Antibody Reactions autoantibody, endothelium, cDNA libraries Autoantigens - analysis Autoantigens - immunology Biological and medical sciences Blotting, Western Cell Line DNA, Complementary - analysis Endothelium, Vascular - chemistry Endothelium, Vascular - cytology Endothelium, Vascular - immunology Fluorescent Antibody Technique, Indirect Gene Library General aspects Humans Immunopathology K562 Cells Medical sciences Molecular Weight |
| title | The Identification of Endothelial Cell Autoantigens |
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