Subcellular localization of epidermal growth factor in human submandibular gland
Epidermal growth factor in human submandibular gland was localized at the subcellular level by means of an immunogold staining method. Labelling was observed in serous acini and ducts. In the acini, gold particles were found within secretory granules, indicating that the growth factor is released in...
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| Veröffentlicht in: | Journal of molecular histology 2000-05, Vol.32 (5), p.291-294 |
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| creator | Cossu, M Perra, M T Piludu, M Lantini, M S |
| description | Epidermal growth factor in human submandibular gland was localized at the subcellular level by means of an immunogold staining method. Labelling was observed in serous acini and ducts. In the acini, gold particles were found within secretory granules, indicating that the growth factor is released into the saliva through granule exocytosis. In the ductal system, the most intense reactivity was revealed in the principal cells of striated ducts. In these cells, an abundant population of small cytoplasmic vesicles was specifically stained. Immunoreactive vesicles were found both apically and basally, suggesting that ductal cells can release their products not only into the saliva but also into the interstitium. |
| doi_str_mv | 10.1023/A:1004036929006 |
| format | Article |
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Labelling was observed in serous acini and ducts. In the acini, gold particles were found within secretory granules, indicating that the growth factor is released into the saliva through granule exocytosis. In the ductal system, the most intense reactivity was revealed in the principal cells of striated ducts. In these cells, an abundant population of small cytoplasmic vesicles was specifically stained. Immunoreactive vesicles were found both apically and basally, suggesting that ductal cells can release their products not only into the saliva but also into the interstitium.</description><identifier>ISSN: 0018-2214</identifier><identifier>ISSN: 1567-2379</identifier><identifier>EISSN: 1567-2387</identifier><identifier>DOI: 10.1023/A:1004036929006</identifier><identifier>PMID: 10939516</identifier><language>eng</language><publisher>Netherlands: Springer Nature B.V</publisher><subject>Adolescent ; Adult ; Cells ; Epidermal Growth Factor - analysis ; Female ; Humans ; Male ; Middle Aged ; Proteins ; Staining and Labeling - methods ; Subcellular Fractions - chemistry ; Submandibular Gland - chemistry ; Submandibular Gland - pathology</subject><ispartof>Journal of molecular histology, 2000-05, Vol.32 (5), p.291-294</ispartof><rights>Kluwer Academic Publishers 2000</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c281t-92e8e8d6d135b60cd141eeb03726adc7f230c83cc039f902b4c35b3f609ce14e3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10939516$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cossu, M</creatorcontrib><creatorcontrib>Perra, M T</creatorcontrib><creatorcontrib>Piludu, M</creatorcontrib><creatorcontrib>Lantini, M S</creatorcontrib><title>Subcellular localization of epidermal growth factor in human submandibular gland</title><title>Journal of molecular histology</title><addtitle>Histochem J</addtitle><description>Epidermal growth factor in human submandibular gland was localized at the subcellular level by means of an immunogold staining method. Labelling was observed in serous acini and ducts. In the acini, gold particles were found within secretory granules, indicating that the growth factor is released into the saliva through granule exocytosis. In the ductal system, the most intense reactivity was revealed in the principal cells of striated ducts. In these cells, an abundant population of small cytoplasmic vesicles was specifically stained. Immunoreactive vesicles were found both apically and basally, suggesting that ductal cells can release their products not only into the saliva but also into the interstitium.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Cells</subject><subject>Epidermal Growth Factor - analysis</subject><subject>Female</subject><subject>Humans</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Proteins</subject><subject>Staining and Labeling - methods</subject><subject>Subcellular Fractions - chemistry</subject><subject>Submandibular Gland - chemistry</subject><subject>Submandibular Gland - pathology</subject><issn>0018-2214</issn><issn>1567-2379</issn><issn>1567-2387</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpdkM1LxDAQxYMo7rp69iYBwVt1krRJ400Wv2BBQT2XNE13s6TNmjaI_vVmdb14enP4vZl5D6FTApcEKLu6uSYAOTAuqQTge2hKCi4yykqxj6YApMwoJfkEHQ3DGgCkEPwQTQhIJgvCp-j5JdbaOBedCth5rZz9UqP1PfYtNhvbmNAph5fBf4wr3Co9-oBtj1exUz0eYp2ksfWPfenSfIwOWuUGc7LTGXq7u32dP2SLp_vH-c0i07QkYyapKU3Z8IawouagG5ITY2pggnLVaNFSBrpkWgOTrQRa5zqBrOUgtSG5YTN08bt3E_x7NMNYdXbYJlG98XGoBBGFzEEk8PwfuPYx9Om3KlW4PUiBJ-psR6VIpqk2wXYqfFZ_TbFvlaZqdg</recordid><startdate>20000501</startdate><enddate>20000501</enddate><creator>Cossu, M</creator><creator>Perra, M T</creator><creator>Piludu, M</creator><creator>Lantini, M S</creator><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>3V.</scope><scope>7QP</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20000501</creationdate><title>Subcellular localization of epidermal growth factor in human submandibular gland</title><author>Cossu, M ; 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Labelling was observed in serous acini and ducts. In the acini, gold particles were found within secretory granules, indicating that the growth factor is released into the saliva through granule exocytosis. In the ductal system, the most intense reactivity was revealed in the principal cells of striated ducts. In these cells, an abundant population of small cytoplasmic vesicles was specifically stained. Immunoreactive vesicles were found both apically and basally, suggesting that ductal cells can release their products not only into the saliva but also into the interstitium.</abstract><cop>Netherlands</cop><pub>Springer Nature B.V</pub><pmid>10939516</pmid><doi>10.1023/A:1004036929006</doi><tpages>4</tpages></addata></record> |
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| subjects | Adolescent Adult Cells Epidermal Growth Factor - analysis Female Humans Male Middle Aged Proteins Staining and Labeling - methods Subcellular Fractions - chemistry Submandibular Gland - chemistry Submandibular Gland - pathology |
| title | Subcellular localization of epidermal growth factor in human submandibular gland |
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