Optical flow-cell multichannel immunosensor for the detection of biological warfare agents
An automated optical flow cell multichannel immunosensor for the detection and identification of toxins, viruses and bacterial particles is presented. A solid phase ELISA, based on a peroxidase label for signal generation and on fused silica capillaries as a support for immobilized antibodies, has b...
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| Veröffentlicht in: | Biosensors & bioelectronics 2000, Vol.14 (10), p.779-784 |
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| container_title | Biosensors & bioelectronics |
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| creator | Koch, Sabine Wolf, Hans Danapel, Christine Feller, Klaus A |
| description | An automated optical flow cell multichannel immunosensor for the detection and identification of toxins, viruses and bacterial particles is presented. A solid phase ELISA, based on a peroxidase label for signal generation and on fused silica capillaries as a support for immobilized antibodies, has been employed for analyte detection and identification. The sensing and signal transducing component of the sensor consists of a light-emitting diode and a photodetector. The device is fitted with three channels allowing the simultaneous detection of three agents. An integrated flow injection analysis system ensures automation of the assay cycles. Data on the detection of the bacterial toxin staphylococcal enterotoxin B (SEB), the bacteriophage M13 as a viral agent, and
Escherichia coli as a bacterial agent are presented. |
| doi_str_mv | 10.1016/S0956-5663(99)00051-2 |
| format | Article |
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Escherichia coli as a bacterial agent are presented.</description><subject>Bacteriophage M13 - isolation & purification</subject><subject>Bacterium</subject><subject>Biological Warfare</subject><subject>Biosensing Techniques</subject><subject>ELISA</subject><subject>Enterotoxins - analysis</subject><subject>Escherichia coli - isolation & purification</subject><subject>Fused silica capillary</subject><subject>Immunoassay</subject><subject>Optical immunosensor</subject><subject>Sensitivity and Specificity</subject><subject>Toxin</subject><subject>Virus</subject><issn>0956-5663</issn><issn>1873-4235</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1LxDAQhoMoun78BKUn0UM1H02zOYksfoHgQb14Cdl0opE2WZNW8d-b3Yp428MwMDwzw_sgdEjwGcGkPn_Ektclr2t2IuUpxpiTkm6gCZkKVlaU8U00-UN20G5K7xkSROJttEOwrHjF6QS9PCx6Z3Rb2DZ8lQbatuiGNo_etPfQFq7rBh8S-BRiYXP1b1A00IPpXfBFsMXchTa8rm586Wh1hEK_gu_TPtqyuk1w8Nv30PP11dPstrx_uLmbXd6Xhknal7ayWmI7bwxjVWVYJanlvG6wECKnqonGsm4q0ARTkIzOgVIsgIlGCsmFZXvoeLy7iOFjgNSrzqVlEu0hDEkJIjjHtVgLUpFVMTrNIB9BE0NKEaxaRNfp-K0IVkv7amVfLdUqKdXKvqJ57-j3wTDvoPm3NerOwMUIQPbx6SCqZBx4A42L2ahqglvz4gesGZSI</recordid><startdate>2000</startdate><enddate>2000</enddate><creator>Koch, Sabine</creator><creator>Wolf, Hans</creator><creator>Danapel, Christine</creator><creator>Feller, Klaus A</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U5</scope><scope>8FD</scope><scope>L7M</scope><scope>7X8</scope></search><sort><creationdate>2000</creationdate><title>Optical flow-cell multichannel immunosensor for the detection of biological warfare agents</title><author>Koch, Sabine ; Wolf, Hans ; Danapel, Christine ; Feller, Klaus A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c392t-f4fa90fbdc3344c3492f556d077700561a096d4ea102e932be2207e37d97957f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Bacteriophage M13 - isolation & purification</topic><topic>Bacterium</topic><topic>Biological Warfare</topic><topic>Biosensing Techniques</topic><topic>ELISA</topic><topic>Enterotoxins - analysis</topic><topic>Escherichia coli - isolation & purification</topic><topic>Fused silica capillary</topic><topic>Immunoassay</topic><topic>Optical immunosensor</topic><topic>Sensitivity and Specificity</topic><topic>Toxin</topic><topic>Virus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Koch, Sabine</creatorcontrib><creatorcontrib>Wolf, Hans</creatorcontrib><creatorcontrib>Danapel, Christine</creatorcontrib><creatorcontrib>Feller, Klaus A</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Biosensors & bioelectronics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Koch, Sabine</au><au>Wolf, Hans</au><au>Danapel, Christine</au><au>Feller, Klaus A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Optical flow-cell multichannel immunosensor for the detection of biological warfare agents</atitle><jtitle>Biosensors & bioelectronics</jtitle><addtitle>Biosens Bioelectron</addtitle><date>2000</date><risdate>2000</risdate><volume>14</volume><issue>10</issue><spage>779</spage><epage>784</epage><pages>779-784</pages><issn>0956-5663</issn><eissn>1873-4235</eissn><abstract>An automated optical flow cell multichannel immunosensor for the detection and identification of toxins, viruses and bacterial particles is presented. A solid phase ELISA, based on a peroxidase label for signal generation and on fused silica capillaries as a support for immobilized antibodies, has been employed for analyte detection and identification. The sensing and signal transducing component of the sensor consists of a light-emitting diode and a photodetector. The device is fitted with three channels allowing the simultaneous detection of three agents. An integrated flow injection analysis system ensures automation of the assay cycles. Data on the detection of the bacterial toxin staphylococcal enterotoxin B (SEB), the bacteriophage M13 as a viral agent, and
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| ispartof | Biosensors & bioelectronics, 2000, Vol.14 (10), p.779-784 |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Bacteriophage M13 - isolation & purification Bacterium Biological Warfare Biosensing Techniques ELISA Enterotoxins - analysis Escherichia coli - isolation & purification Fused silica capillary Immunoassay Optical immunosensor Sensitivity and Specificity Toxin Virus |
| title | Optical flow-cell multichannel immunosensor for the detection of biological warfare agents |
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