Recombinant Fibrinogen Studies Reveal That Thrombin Specificity Dictates Order of Fibrinopeptide Release

During cleavage of fibrinogen by thrombin, fibrinopeptide A (FpA) release precedes fibrinopeptide B (FpB) release. To examine the basis for this ordered release, we synthesized A′β fibrinogen, replacing FpB with a fibrinopeptide A-like peptide, FpA′ (G14V). Analyses of fibrinopeptide release from A′...

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Veröffentlicht in:	The Journal of biological chemistry 2000-08, Vol.275 (33), p.25239-25246
Hauptverfasser:	Mullin, Jennifer L., Gorkun, Oleg V., Binnie, Cameron G., Lord, Susan T.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals CHO Cells Chromatography, High Pressure Liquid Cloning, Molecular Cricetinae Electrophoresis, Polyacrylamide Gel Fibrinogen - metabolism Fibrinopeptide B - metabolism Humans Kinetics Microscopy, Electron, Scanning Models, Biological Peptides - metabolism Plasmids - metabolism Recombinant Proteins - metabolism Substrate Specificity Thrombin - metabolism Time Factors
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